Jan Wilschut

Effect of a second, booster, influenza vaccination on antibody responses in quiescent systemic lupus erythematosus: an open, prospective, controlled study (2009)

Holvast, Albert, Van Assen, Sander, De Haan, Aalzen, Huckriede, Anke, Benne, Cornelis A., Westra, Johanna, ...

Objective. In SLE, a decreased antibody response on influenza vaccination has been reported. In this study, we assessed whether a booster vaccination could improve antibody responses, as determined...

Profylactische vaccins ter voorkoming van HPV-gerelateerde kanker (2008)

Nijman, Hans, Reesink-Peters, Nathalie, Postma, Maarten, Daemen, Toos, Wilschut, Jan, Zee, Ate Van Der

Slow fusion of liposomes composed of membrane-spanning lipids (1997)

Elferink, Marieke G.L., Breemen, Jan Van, Konings, Wil N., Driessen, Arnold J.M., Wilschut, Jan

The fusion characteristics of large unilamellar liposomes composed of bipolar tetraether lipids extracted from the thermophilic archaeon Sulfolobus acidocaldarius, was investigated. These lipids span...

Vectors based on Semliki Forest virus for rapid and efficient gene transfer into non-endothelial cardiovascular cells: comparison to adenovirus (1997)

Roks, Anton J.M, Pinto, Yigal M, Paul, Martin, Pries, Frens, Stula, Martin, Eschenhagen, Thomas, ...

Objective: Replication-deficient, recombinant adenovirus is used as a carrier for gene transfer, but it is unspecific and the onset of transgene expression is relatively late. Here, we evaluated the...

A synthetic peptide corresponding to the C-terminal 25 residues of phage MS2 coded lysis protein dissipates the protonmotive force in Escherichia coli membrane vesicles by generating hydrophilic pores (1988)

Goessens, Wil H.F., Driessen, Arnold J.M., Wilschut, Jan, Duin, Jan Van

The RNA phage MS2 encodes a protein, 75 amino acids long, that is necessary and sufficient for lysis of the host cell. DNA deletion analysis has shown that the lytic activity is confined to the...

Low pH-induced Fusion of Liposomes with Membrane Vesicles Derived from Bacillus subtilis (1985)

Driessen, Arnold J.M., Hoekstra, Dick, Scherphof, Gerrit, Kalicharan, Ruby D., Wilschut, Jan

We have investigated the pH-dependent interaction between large unilamellar phospholipid vesicles (liposomes) and membrane vesicles derived from Bacillus subtilis, utilizing a fluorescent assay based...

Role of Hemagglutinin Surface Density in the Initial Stages of Influenza Virus Fusion: Lack of Evidence for Cooperativity

Günther-Ausborn, Susanne, Schoen, Pieter, Bartoldus, Ingrid, Wilschut, Jan, Stegmann, Toon

Membrane fusion mediated by influenza virus hemagglutinin (HA) is believed to proceed via the cooperative action of multiple HA trimers. To determine the minimal number of HA trimers required to...

Low-pH-Dependent Fusion of Sindbis Virus with Receptor-Free Cholesterol- and Sphingolipid-Containing Liposomes

Smit, Jolanda M., Bittman, Robert, Wilschut, Jan

There is controversy as to whether the cell entry mechanism of Sindbis virus (SIN) involves direct fusion of the viral envelope with the plasma membrane at neutral pH or uptake by receptor-mediated...

PE2 Cleavage Mutants of Sindbis Virus: Correlation between Viral Infectivity and pH-Dependent Membrane Fusion Activation of the Spike Heterodimer

Smit, Jolanda M., Klimstra, William B., Ryman, Kate D., Bittman, Robert, Johnston, Robert E., Wilschut, Jan

The spike glycoprotein E2 of Sindbis virus (SIN) is synthesized in the infected cell as a PE2 precursor protein, which matures through cleavage by a cellular furin-like protease. Previous work has...

A Single Mutation in the E2 Glycoprotein Important for Neurovirulence Influences Binding of Sindbis Virus to Neuroblastoma Cells

Lee, Peiyu, Knight, Ronald, Smit, Jolanda M., Wilschut, Jan, Griffin, Diane E.

The amino acid at position 55 of the E2 glycoprotein (E255) of Sindbis virus (SV) is a critical determinant of SV neurovirulence in mice. Recombinant virus strain TE (E255 = histidine) differs only...

Adaptation of Alphaviruses to Heparan Sulfate: Interaction of Sindbis and Semliki Forest Viruses with Liposomes Containing Lipid-Conjugated Heparin

Smit, Jolanda M., Waarts, Barry-Lee, Kimata, Koji, Klimstra, William B., Bittman, Robert, Wilschut, Jan

Passage of Sindbis virus (SIN) in BHK-21 cells has been shown to select for virus mutants with high affinity for the glycosaminoglycan heparan sulfate (HS). Three loci in the viral spike protein E2...

Reversible Acid-Induced Inactivation of the Membrane Fusion Protein of Semliki Forest Virus

Waarts, Barry-Lee, Smit, Jolanda M., Aneke, Onwuchekwa J. C., McInerney, Gerald M., Liljeström, Peter, Bittman, Robert, ...

Previously, it has been shown that the exposure of Semliki Forest virus (SFV) to a mildly acidic environment induces a rapid and complete loss of the ability of the virus to bind and fuse to target...

Role of Hemagglutinin Surface Density in the Initial Stages of Influenza Virus Fusion: Lack of Evidence for Cooperativity

Günther-Ausborn, Susanne, Schoen, Pieter, Bartoldus, Ingrid, Wilschut, Jan, Stegmann, Toon

Membrane fusion mediated by influenza virus hemagglutinin (HA) is believed to proceed via the cooperative action of multiple HA trimers. To determine the minimal number of HA trimers required to...

Low-pH-Dependent Fusion of Sindbis Virus with Receptor-Free Cholesterol- and Sphingolipid-Containing Liposomes

Smit, Jolanda M., Bittman, Robert, Wilschut, Jan

There is controversy as to whether the cell entry mechanism of Sindbis virus (SIN) involves direct fusion of the viral envelope with the plasma membrane at neutral pH or uptake by receptor-mediated...

PE2 Cleavage Mutants of Sindbis Virus: Correlation between Viral Infectivity and pH-Dependent Membrane Fusion Activation of the Spike Heterodimer

Smit, Jolanda M., Klimstra, William B., Ryman, Kate D., Bittman, Robert, Johnston, Robert E., Wilschut, Jan

The spike glycoprotein E2 of Sindbis virus (SIN) is synthesized in the infected cell as a PE2 precursor protein, which matures through cleavage by a cellular furin-like protease. Previous work has...

A Single Mutation in the E2 Glycoprotein Important for Neurovirulence Influences Binding of Sindbis Virus to Neuroblastoma Cells

Lee, Peiyu, Knight, Ronald, Smit, Jolanda M., Wilschut, Jan, Griffin, Diane E.

The amino acid at position 55 of the E2 glycoprotein (E255) of Sindbis virus (SV) is a critical determinant of SV neurovirulence in mice. Recombinant virus strain TE (E255 = histidine) differs only...

Adaptation of Alphaviruses to Heparan Sulfate: Interaction of Sindbis and Semliki Forest Viruses with Liposomes Containing Lipid-Conjugated Heparin

Smit, Jolanda M., Waarts, Barry-Lee, Kimata, Koji, Klimstra, William B., Bittman, Robert, Wilschut, Jan

Passage of Sindbis virus (SIN) in BHK-21 cells has been shown to select for virus mutants with high affinity for the glycosaminoglycan heparan sulfate (HS). Three loci in the viral spike protein E2...

Reversible Acid-Induced Inactivation of the Membrane Fusion Protein of Semliki Forest Virus

Waarts, Barry-Lee, Smit, Jolanda M., Aneke, Onwuchekwa J. C., McInerney, Gerald M., Liljeström, Peter, Bittman, Robert, ...

Previously, it has been shown that the exposure of Semliki Forest virus (SFV) to a mildly acidic environment induces a rapid and complete loss of the ability of the virus to bind and fuse to target...

Cellular gene transfer mediated by influenza virosomes with encapsulated plasmid DNA

De Jonge, Jørgen, Leenhouts, Johanna M., Holtrop, Marijke, Schoen, Pieter, Scherrer, Peter, Cullis, Pieter R., ...

Reconstituted influenza virosomes (virus membrane envelopes) have been used previously to deliver pDNA (plasmid DNA) bound to their external surface to a variety of target cells. Although high...

Characterization of the Early Events in Dengue Virus Cell Entry by Biochemical Assays and Single-Virus Tracking▿ †

Van Der Schaar, Hilde M., Rust, Michael J., Waarts, Barry-Lee, Kuhn, Richard J., Wilschut, Jan, ...

In this study, we investigated the cell entry characteristics of dengue virus (DENV) type 2 strain S1 on mosquito, BHK-15, and BS-C-1 cells. The concentration of virus particles measured by...

Superior Immunogenicity of Inactivated Whole Virus H5N1 Influenza Vaccine is Primarily Controlled by Toll-like Receptor Signalling

Geeraedts, Felix, Goutagny, Nadege, Hornung, Veit, Severa, Martina, De Haan, Aalzen, Pool, Judith, ...

In the case of an influenza pandemic, the current global influenza vaccine production capacity will be unable to meet the demand for billions of vaccine doses. The ongoing threat of an H5N1 pandemic...

Dissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells

Van Der Schaar, Hilde M., Rust, Michael J., Chen, Chen, Wilschut, Jan, Zhuang, Xiaowei, ...

Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used...

A Therapeutic Antibody against West Nile Virus Neutralizes Infection by Blocking Fusion within Endosomes

Thompson, Bruce S., Moesker, Bastiaan, Smit, Jolanda M., Wilschut, Jan, Diamond, Michael S., Fremont, Daved H.

Defining the precise cellular mechanisms of neutralization by potently inhibitory antibodies is important for understanding how the immune system successfully limits viral infections. We recently...