Manuel Sánchez; Centro De Investigación Básica, NPDD–Merck ResearchLaboratories, Merck Sharp & Dohme De España S.A., Madrid, Spain, María Francisca Vicente; Centro De Investigación Básica, NPDD–Merck Research Laboratories, Merck Sharp & Dohme De España S.A., Madrid, Spain, Emilia Cercenado; Servicio De Microbiología, Hospital General Universitario “Gregorio Marañón”, Madrid, Spain, Miguel A. De Pedro; Departamento De Biología Molecular, CBM-UAM, Madrid, Spain, Pilar Gómez; Centro De Investigación Básica, NPDD–Merck ResearchLaboratories, Merck Sharp & Dohme De España S.A., Madrid, Spain; And Departamento De Biología Molecular, CBM-UAM, Madrid, Spain, Renata Moreno; Departamento De Biología Molecular, CBM-UAM, Madrid, Spain, ...
A total of 12 non-epidemiologically related clinical isolates of Streptococcus mitis that showed different levels of resistance to penicillin were studied. Membrane-protein profiles and...
Blas, Emilio, Cava, Felipe, López-Viñas, Eduardo, Mendieta, Jesús, Berenguer, José
Supplementary material available
Zafra, Olga, Cava, Felipe, Blasco, Francis, Magalon, Axel, Berenguer, José
The nar operon, coding for the respiratory nitrate reductase of Thermus thermophilus (NRT), encodes a di-heme b-type (NarJ) and a di-heme c-type (NarC) cytochrome. The role of both cytochromes and...
Moreno, Renata, Hidalgo, Aurelio, Cava, Felipe, Fernández-Lafuente, Roberto, Manuel Guisán, José, Berenguer, José
The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive...
Perales, Celia, Cava, Felipe, Meijer, Wilfried J.J., Berenguer, José
Bacterial single-stranded DNA-binding proteins (SSBs) are required for DNA replication and repair. We have over-expressed and purified the native form and two His-tagged fusions of the SSB from...
Temperature-dependent hypermutational phenotype in recA mutants of Thermus thermophilus HB27 (2003)
Castán, Pablo, Casares, Lorena, Barbé, Jordi, Berenguer, José
The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (delta-recA) derivatives. Transcription of recA in this extreme thermophile was...
Perales, Celia, Cava, Felipe, Meijer, Wilfried J. J., Berenguer, José
Bacterial single‐stranded DNA‐binding proteins (SSBs) are required for DNA replication and repair. We have over‐expressed and purified the native form and two His‐tagged fusions...
Vazquez-Tello, Alejandro, Castán, Pablo, Moreno, Renata, Smith, James M., Berenguer, José, Cedergren, Robert
The catalytic hammerhead structure has been found in association with repetitive DNA from several animals, including salamanders, crickets and schistosomes, and functions to process in cis the long...
Castán, Pablo, De Pedro, Miguel A., Risco, Cristina, Vallés, Cristina, Fernández, Luis A., Schwarz, Heinz, ...
The role of the 5′ untranslated region (5′UTR) of the S-layer gene from Thermus thermophilus was analyzed through the isolation of Δ5′UTR mutants. In these mutants the half-life of splA mRNA...
Vazquez-Tello, Alejandro, Castán, Pablo, Moreno, Renata, Smith, James M., Berenguer, José, Cedergren, Robert
The catalytic hammerhead structure has been found in association with repetitive DNA from several animals, including salamanders, crickets and schistosomes, and functions to process in cis the long...
Ramírez-Arcos, Sandra, Fernández-Herrero, Luis A., Marín, Irma, Berenguer, José
Despite the fact that the extreme thermophilic bacteria belonging to the genus Thermus are classified as strict aerobes, we have shown that Thermus thermophilus HB8 (ATCC 27634) can grow...
Ramírez, Sandra, Moreno, Renata, Zafra, Olga, Castán, Pablo, Vallés, Cristina, Berenguer, José
Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other...
Temperature-Dependent Hypermutational Phenotype in recA Mutants of Thermus thermophilus HB27
Castán, Pablo, Casares, Lorena, Barbé, Jordi, Berenguer, José
The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (ΔrecA) derivatives. Transcription of recA in this extreme thermophile was induced...
Berenguer, José, De Pedro, Miguel A., Vázquez, David
Cell lysis can be induced in Escherichia coli by the combined use of the β-lactam antibiotics nocardicin A and mecillinam. Apparently, the induction of cell lysis occurs by a sequential mechanism in...
Perales, Celia, Cava, Felipe, Meijer, Wilfried J. J., Berenguer, José
Bacterial single-stranded DNA-binding proteins (SSBs) are required for DNA replication and repair. We have over-expressed and purified the native form and two His-tagged fusions of the SSB from...
Hidalgo, Aurelio, Betancor, Lorena, Moreno, Renata, Zafra, Olga, Cava, Felipe, Fernández-Lafuente, Roberto, ...
Thermostable Mn-dependent catalases are promising enzymes in biotechnological applications as H2O2-detoxifying systems. We cloned the genes encoding Mn-dependent catalases from Thermus thermophilus...
Moreno, Renata, Hidalgo, Aurelio, Cava, Felipe, Fernández-Lafuente, Roberto, Guisán, José Manuel, Berenguer, José
The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive...
High-Level Overproduction of His-Tagged Tth DNA Polymerase in Thermus thermophilus
Moreno, Renata, Haro, Amparo, Castellanos, Angel, Berenguer, José
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was...
Castán, Pablo, De Pedro, Miguel A., Risco, Cristina, Vallés, Cristina, Fernández, Luis A., Schwarz, Heinz, ...
The role of the 5′ untranslated region (5′UTR) of the S-layer gene from Thermus thermophilus was analyzed through the isolation of Δ5′UTR mutants. In these mutants the half-life of splA mRNA...
Vazquez-Tello, Alejandro, Castán, Pablo, Moreno, Renata, Smith, James M., Berenguer, José, Cedergren, Robert
The catalytic hammerhead structure has been found in association with repetitive DNA from several animals, including salamanders, crickets and schistosomes, and functions to process in cis the long...
Ramírez-Arcos, Sandra, Fernández-Herrero, Luis A., Marín, Irma, Berenguer, José
Despite the fact that the extreme thermophilic bacteria belonging to the genus Thermus are classified as strict aerobes, we have shown that Thermus thermophilus HB8 (ATCC 27634) can grow...
Ramírez, Sandra, Moreno, Renata, Zafra, Olga, Castán, Pablo, Vallés, Cristina, Berenguer, José
Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other...
Temperature-Dependent Hypermutational Phenotype in recA Mutants of Thermus thermophilus HB27
Castán, Pablo, Casares, Lorena, Barbé, Jordi, Berenguer, José
The recA gene from Thermus thermophilus HB27 was cloned and engineered to obtain insertion (recA::kat) and deletion (ΔrecA) derivatives. Transcription of recA in this extreme thermophile was induced...
Berenguer, José, De Pedro, Miguel A., Vázquez, David
Cell lysis can be induced in Escherichia coli by the combined use of the β-lactam antibiotics nocardicin A and mecillinam. Apparently, the induction of cell lysis occurs by a sequential mechanism in...
Perales, Celia, Cava, Felipe, Meijer, Wilfried J. J., Berenguer, José
Bacterial single-stranded DNA-binding proteins (SSBs) are required for DNA replication and repair. We have over-expressed and purified the native form and two His-tagged fusions of the SSB from...
Hidalgo, Aurelio, Betancor, Lorena, Moreno, Renata, Zafra, Olga, Cava, Felipe, Fernández-Lafuente, Roberto, ...
Thermostable Mn-dependent catalases are promising enzymes in biotechnological applications as H2O2-detoxifying systems. We cloned the genes encoding Mn-dependent catalases from Thermus thermophilus...
Moreno, Renata, Hidalgo, Aurelio, Cava, Felipe, Fernández-Lafuente, Roberto, Guisán, José Manuel, Berenguer, José
The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive...
High-Level Overproduction of His-Tagged Tth DNA Polymerase in Thermus thermophilus
Moreno, Renata, Haro, Amparo, Castellanos, Angel, Berenguer, José
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was...
Blas-Galindo, Emilio, Cava, Felipe, López-Viñas, Eduardo, Mendieta, Jesús, Berenguer, José
A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal...