Gaur, Snigdha, Heckman, Joyce E., Burke, John M.
The hairpin ribozyme acts as a reversible, site-specific endoribonuclease that ligates much more rapidly than it cleaves cognate substrate. While the reaction pathway for ligation is the reversal of...
Kreader, Carol A., Heckman, Joyce E.
We have isolated and characterized a Neurospora crassa gene homologous to the yeast CYH2 gene encoding L29, a cycloheximide sensitivity-conferring protein of the cytoplasmic ribosome. The cloned...
Structural basis for heterogeneous kinetics: Reengineering the hairpin ribozyme
Esteban, José A., Walter, Nils G., Kotzorek, Gerd, Heckman, Joyce E., Burke, John M.
The RNA cleavage reaction catalyzed by the hairpin ribozyme shows biphasic kinetics, and chase experiments show that the slow phase of the reaction results from reversible substrate binding to an...
Functional involvement of G8 in the hairpin ribozyme cleavage mechanism
Pinard, Robert, Hampel, Ken J., Heckman, Joyce E., Lambert, Dominic, Chan, Philip A., Major, Francois, ...
The catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are integral to the polyribonucleotide chain. We describe experiments that place G8, a critical...
Heckman, Joyce E., Sarnoff, Joshua, Alzner-DeWeerd, Birgit, Yin, Samuel, RajBhandary, Uttam L.
We report the sequences of Neurospora crassa mitochondrial alanine, leucine1, leucine2, threonine, tryptophan, and valine tRNAs. On the basis of the anticodon sequences of these tRNAs and of a...
Structural basis for heterogeneous kinetics: Reengineering the hairpin ribozyme
Esteban, José A., Walter, Nils G., Kotzorek, Gerd, Heckman, Joyce E., Burke, John M.
The RNA cleavage reaction catalyzed by the hairpin ribozyme shows biphasic kinetics, and chase experiments show that the slow phase of the reaction results from reversible substrate binding to an...
Functional involvement of G8 in the hairpin ribozyme cleavage mechanism
Pinard, Robert, Hampel, Ken J., Heckman, Joyce E., Lambert, Dominic, Chan, Philip A., Major, Francois, ...
The catalytic determinants for the cleavage and ligation reactions mediated by the hairpin ribozyme are integral to the polyribonucleotide chain. We describe experiments that place G8, a critical...
Heckman, Joyce E., Sarnoff, Joshua, Alzner-DeWeerd, Birgit, Yin, Samuel, RajBhandary, Uttam L.
We report the sequences of Neurospora crassa mitochondrial alanine, leucine1, leucine2, threonine, tryptophan, and valine tRNAs. On the basis of the anticodon sequences of these tRNAs and of a...
Gaur, Snigdha, Heckman, Joyce E., Burke, John M.
The hairpin ribozyme acts as a reversible, site-specific endoribonuclease that ligates much more rapidly than it cleaves cognate substrate. While the reaction pathway for ligation is the reversal of...