K. Watanabe

Details der Publikationsliste

Zeitraum

1953 - 9999

Anzahl

516

Co-Autoren

Study on Steerability of Articulated Tracked Vehicles, (9999)

Watanabe,K., Kitano,M.

In previous paper, we have presented available equations of motion for analyzing the plane motion of the couple(non-articulated) tracked vehicles with tow-pins. In this paper, a mathematical analog...

Fabrication of Periodically Reversed Domain Structure for Second-Harmonic-Generation in LiNbO3 by Applying Voltage, (9999)

Yamada, M., Nada, N., Watanabe, K.

Recently there has been much interest in quasi-phase-matched(QPM)second harmonic generation (SHG) to obtain coherent blue light in LiNbO3 waveguide. Several techniques for the domain reversal of...

DYNAMIC EFFECTS AND INFORMATION QUANTIFIERS OF STATISTICAL MEMORY OF MEG’S SIGNALS AT PHOTOSENSITIVE EPILEPSY (2009)

R. M. Yulmetyev, E. V. Khusaenova, D. G. Yulmetyeva, P. Hänggi, S. Shimojo, K. Watanabe, ...

(Communicated by Ying-Cheng Lai) Abstract. The time series analysis of magnetoencephalographic (MEG) signals is very important both for basic brain research and for medical diagnosis and treatment....

Digital Photogrammetry for the Documentation of Structural Damage in Earthen Archaeological Sites: the Case of Ajina Tepa, Tajikistan (2009)

Fujii, Y., Fodde, Enrico, Watanabe, K., Murakami, K.

Ajina Tepa is one of the most important archaeological sites in central Asia and it was fully excavated in the 1960s using modern documentation techniques. The UNESCO/Japan Trust Fund project...

$^{63/65}$Cu- and $^{35/37}$Cl-NMR Studies of Triplet Localization in the Quantum Spin System NH$_4$CuCl$_3$ (2009)

Inoue, H., Tani, S., Hosoya, S., Inokuchi, K., Fujiwara, T., Saito, T., ...

$^{63/65}$Cu- and $^{35/37}$Cl-NMR experiments were performed to investigate triplet localization in the $S=1/2$ dimer compound NH$_4$CuCl$_3$, which shows magnetization plateaus at one-quarter and...

Dynamic effects and information quantifiers of statistical memory of MEG's signals at photosensitive epilepsy (2009)

Yulmetyev, R. M., Khusaenova, E. V., Yulmetyeva, D. G., Hänggi, P., Shimojo, S., Watanabe, K., ...

The time series analysis of magnetoencephalographic (MEG) signals is very important both for basic brain research and for medical diagnosis and treatment. Here we discuss the crucial role of...

Preservation of Earthen Sites in Remote Areas: the Buddhist Monastery of Ajina Tepa, Tajikistan (2009)

Fodde, Enrico, Watanabe, K., Fujii, Y.

The Buddhist monastery of Ajina Tepa is one of the most signifi cant in Central Asia as it was fully excavated using up-to-date archaeological methods and was extensively documented. The site is a...

Technical Note Formal blind intercomparison of OH measurements: results from the international campaign HOxComp (2009)

E. Schlosser, T. Brauers, H. Fuchs, R. Häseler, A. Hofzumahaus, ...

Hydroxyl radicals (OH) are the major oxidizing species in the troposphere. Because of their central importance, absolute measurements of their concentrations are needed to validate chemical...

Technical Note: Formal blind intercomparison of OH measurements: results from the international campaign HOxComp (2009)

E. Schlosser, T. Brauers, H. Fuchs, R. Häseler, A. Hofzumahaus, ...

Hydroxyl radicals (OH) are the major oxidizing species in the troposphere. Because of their central importance, absolute measurements of their concentrations are needed to validate chemical...

Statistical memory of MEG signals at photosensitive epilepsy (2008)

Yulmetyev, R. M., Khusaenova, E. V., Yulmetyeva, D. G., Hänggi, P., Shimojo, S., Watanabe, K., ...

Here we discuss the remarkable role of the statistical memory effects in the human brain functioning at photosensitive epilepsy (PSE). We have analyzed three independent statistical memory...

A cheap liquid additive from acetone for strong in-field Jc enhancement in MgB2 conductors (2008)

Wang, Dongliang, Ma, Yanwei, Gao, Zhaoshun, Zhang, Xianping, Wang, Lei, Awaji, S., ...

We report on significant flux pinning enhancement in MgB2/Fe tapes that has been easily obtained by a simple and cheap route using acetone as both an efficient ball-milling medium and liquid additive...

Photon correlation in GaAs self-assembled quantum dots (2008)

Kuroda, T., Abbarchi, M., Mano, T., Watanabe, K., Yamagiwa, M., Kuroda, K., ...

We report on photon coincidence measurement in a single GaAs self-assembled quantum dot (QD) using a pulsed excitation light source. At low excitation, when a neutral exciton line was present in the...

Mechanism of c-axis orientation of L1(0) FePt in nanostructured FePt/B2O3 thin films (2008)

Ichitsubo, T, Tojo, S, Uchihara, T, Matsubara, E, Fujita, A, Takahashi, K, ...

In oder to obtain a high-density magnetic recording medium using an L10 ferromagnetic ordered alloy, it is imperative to make its c-axis perpendicular to the thin film. In this work, we have...

Hollow carbon spheres as an efficient dopant for enhancing critical current density of MgB2 based tapes (2008)

Gao, Zhaoshun, Ma, Yanwei, Zhang, Xianping, Wang, Dongliang, Wang, Junhong, Watanabe, K., ...

A significant enhancement of Jc and Hirr in MgB2 tapes has been achieved by the in situ powder-in-tube method utilizing hollow carbon spheres (HCS) as dopants. At 4.2 K, the transport Jc for the 850C...

Evaporation Measurement for the Preservation of the Buddhist Monastery of Ajina Tepa, Tajikistan (2008)

Watanabe, K., Maung, M., Fujii, Y., Fodde, Enrico

One of the decay mechanisms of historical earthen walls of Ajina Tepa (Tajikistan) is represented by salt crystallization. Halite, calcite and gypsum are found in abundance on the historical walls....

A combination of octanoate and oleate promotes in vitro differentiation of porcine intramuscular adipocytes (2008)

Sanosaka, M., Minashima, T., Suzuki, K., Watanabe, K., Ohwada, Shyuichi, Hagino, A., ...

To understand the relationship between intramuscular adipogenesis in the pig and the supply fatty acids, we established a clonal porcine intramuscular preadipocyte (PIP) line from the marbling muscle...

A Chaotic Memory Search Model Based on Associative Dynamics Using Features in Stored Patterns (2008)

M. Obayashi, K. Watanabe, M. Obayashi

A new chaotic memory search model based on associative dynamics using features in stored patterns is proposed. In the present paper, two kinds of features are considered; external and internal ones.

Conservation and Documentation of the Buddhist Monastery of Ajina Tepa, Tajikistan: Heritage of the Silk Roads (2008)

Fodde, Enrico, Watanabe, K., Fujii, Y.

The paper describes the documentation and conservation activities carried out in the site of Ajina Tepa, by making an inspection of: damage assessment, field laboratory analysis for the selection of...

Anterior pituitary progenitor cells express costimulatory molecule 4Ig-B7-H3 (2008)

Nagai, Yasuhiro, Aso, H., Ogasawara, Hideki, Tanaka, S., Taketa, Y., Watanabe, K., ...

Stem/Progenitor cells in the postnatal pituitary gland are embedded in a marginal cell layer around Rathke’s pouch. However, the nature and behavior of anterior pituitary progenitor cells remain...

Strong influence of precursor powder on the critical current density of Fe-sheathed MgB2 tapes (2007)

Wang, Dongliang, Ma, Yanwei, Yu, Zhengguang, Gao, Zhaoshun, Zhang, Xianping, Watanabe, K., ...

The effect of the quality of starting powders on the microstructure and superconducting properties of in-situ processed Fe-sheathed MgB2 tapes has been investigated. Three different types of...

Relaxation and phase space singularities in time series of human magnetoencephalograms as indicator of photosensitive epilepsy (2007)

Yulmetyev, R. M., Hänggi, P., Yulmetyeva, D.G., Shimojo, S., Khusaenova, E. V., Watanabe, K., ...

To analyze the crucial role of fluctuation and relaxation effects for the function of the human brain we studied some statistical quantifiers that support the information characteristics of...

NMR Study on the Vortex Slush Phase in Organic Superconductor \kappa-(BEDT-TTF)2Cu(NCS)2 (2007)

Urano, M., Tonishi, J., Inoue, H., Saito, T., Fujiwara, T., Chiku, H., ...

The vortex state in a single crystal of the layered organic superconductor \kappa-(BEDT-TTF2Cu(NCS)2, where BEDT-TTF (or ET) is bis(ethylenedithio)tetrathiafulvalene, was studied by 1H-NMR. Under a...

Conversion efficiency of the high-temperature combustion technique for dissolved organic carbon and total dissolved nitrogen analysis (2007)

Watanabe, K., Badr, E-S., Pan, X., Achterberg, E.P.

The measurements of dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) in seawater are key in global change and coastal eutrophication studies. Nowadays, the high-temperature...

Topology Optimization Based on Immune Algorithm and Multigrid Method (2007)

Watanabe, K., Campelo, F., Igarashi, H.

This paper describes topology optimization of electromagnetic systems using the multigrid method. The topology optimization employs uniform rectangular finite elements, in which the multigrid method...

Topology Optimization Based on Immune Algorithm and Multigrid Method (2007)

Watanabe, K., Campelo, F., Igarashi, H.

This paper describes topology optimization of electromagnetic systems using the multigrid method. The topology optimization employs uniform rectangular finite elements, in which the multigrid method...

Amplification of Quantum Meson Modes in the Late Time of the Chiral Phase Transition (2007)

Watanabe, K., Tsue, Y.

We investigate the time evolution of the quantum meson modes in the late time of chiral phase transition. In particular, it is shown that there exists a possible solution to the equation of motion...

Strongly enhanced critical current density in Fe-sheathed MgB2 tapes by stearic acid and stearate doping (2007)

Gao, Zhaoshun, Ma, Yanwei, Zhang, Xianping, Wang, Dongliang, Yu, Zhengguang, Watanabe, K., ...

Fe-sheathed MgB2 tapes with cheap stearic acid, Mg stearate and Zn stearate doping were prepared through the in situ powder-in-tube method. It is found that Jc, Hirr, and Hc2 were significantly...

Nine acetylenic alcohols isolated from the Okinawan marine sponge of the genus Petrosia (Strongylophora). (2007)

Watanabe, K., Mori, G., Iguchi, K., Suzuki, M.

Nine new acetylenic alcohols 1–9 were isolated from a marine sponge belonging to the genus Petrosia (Strongylophora). The structures were elucidated mainly based on the analysis of one-and...

Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice (2007)

Miyazawa, K., Kanaya, T., Tanaka, S., Takakura, I., Watanabe, K., Ohwada, S., ...

The gastrointestinal tract is thought to be the main site of entry for the pathological isoform of the prion protein (PrPSc). Prion diseases are believed to result from a conformational change of the...

c ○ World Scientific Publishing Company STATISTICAL MEMORY OF MEG SIGNALS AT PHOTOSENSITIVE EPILEPSY (2007)

R. M. Yulmetyev, E. V. Khusaenova, P. H Änggi, S. Shimojo, K. Watanabe, J. Bhattacharya

Here we discuss the remarkable role of the statistical memory effects in the human brain functioning at photosensitive epilepsy (PSE). We have analyzed three independent statistical memory...

Final-state read-out of exciton qubits by observing resonantly excited photoluminescence in quantum dots (2006)

Kuroda, K., Kuroda, T., Watanabe, K., Mano, T., Sakoda, K., Kido, G., ...

We report on a new approach to detect excitonic qubits in semiconductor quantum dots by observing spontaneous emissions from the relevant qubit level. The ground state of excitons is resonantly...

Relaxation and phase space singularities in time series of human magnetoencephalograms as indicator of photosensitive epilepsy (2006)

Yulmetyev, R. M., Hänggi, P., Yulmetyeva, D. G., Shimojo, S., Khusaenova, E. V., Watanabe, K., ...

To analyze the crucial role of fluctuation and relaxation effects for the function of the human brain we studied some statistical quantifiers that support the information characteristics of...

Improved critical current densities in MgB2 tapes with ZrB2 doping (2006)

Zhang, Xianping, Gao, Zhaoshun, Wang, Dongliang, Yu, Zhengguang, Ma, Yanwei, Awaji, S., ...

MgB2/Fe tapes with 2.5-15 at.% ZrB2 additions were prepared through the in situ powder-in-tube method. Compared to the pure tape, a significant improvement in the in-field critical current density Jc...

Improved properties of epitaxial YNixMn1-xO3 films by annealing under high magnetic fields (2006)

Ma, Yanwei, Xu, Aixia, Li, Xiaohang, Guilloux-Viry, M., Pena, O., Awaji, S., ...

The effect of annealing under a magnetic field on the microstructure and properties of YNixMn1-xO3 (x=0.33 and 0.5) films has been investigated. It is found that the ferromagnetic transition...

Cu-NMR study on the disordered quantum spin magnet with the Bose-glass ground state (2006)

Fujiwara, T., Inoue, H., Oosawa, A., Tsunoda, R., Goto, T., Suzuki, T., ...

Cu-NMR study has been performed on the disordered spin-gap system Tl1-xKxCuCl3 In the high-field H > HC=\Delta/\mu_B, where \Delta is the spin-gap, the hyperfine field becomes extremely inhomogeneous...

Design consideration for high-energy-resolution neutron spectrometer based on associated particle detection using proton recoil telescope and time-of-flight technique for ITER (2006)

Naoi, N., Asai, K., Iguchi, T., Watanabe, K., Kawarabayashi, J., Nishitani, T.

For ion temperature measurement in the ITER high power operation phase, we propose a promising high-energy-resolution neutron spectrometer based on the associated particle detection using a proton...

Novel concept of time-of-flight neutron spectrometer for measurement of the D/T burning ratio in the ITER (2006)

Asai, K., Naoi, N., Iguchi, T., Watanabe, K., Kawarabayashi, J., Nishitani, T.

A time-of-flight (TOF) neutron spectrometer is a candidate for the measurement of the D/T burning ratio in the International Thermonuclear Experimental Reactor (ITER). In ITER high-power experiments,...

Large irreversibility field in nanoscale C-doped MgB2/Fe tape conductors (2006)

Ma, Yanwei, Zhang, Xianping, Awaji, S., Gao, Zhaoshun, Wang, Dongliang, Yu, Zhengguang, ...

We investigated the effect of nanoscale-C doping on the critical current density Jc and irreversibility field Birr of Fe-sheathed MgB2 tapes prepared by the in-situ powder-in-tube method. The tapes...

Effect of Sodium Valproate on Neuromagnetic Responses to Chromatic Flicker: Implication for Photosensitivity (2006)

K Watanabe, R Sayres, S Shimojo, T Imada, K Nihei

The effect of oral administration of sodium valproate in normal subjects was evaluated using whole-scalp magnetoencephalography, with results compared to the effect of sodium valproate in...

Effect of Sodium Valproate on Neuromagnetic Responses to Chromatic Flicker: Implication for Photosensitivity (2006)

K Watanabe, R Sayres, S Shimojo, T Imada, K Nihei

The effect of oral administration of sodium valproate in normal subjects was evaluated using whole-scalp magnetoencephalography, with results compared to the effect of sodium valproate in...

Effect of Sodium Valproate on Neuromagnetic Responses to Chromatic Flicker: Implication for Photosensitivity (2006)

K Watanabe, R Sayres, S Shimojo, T Imada, K Nihei

The effect of oral administration of sodium valproate in normal subjects was evaluated using whole-scalp magnetoencephalography, with results compared to the effect of sodium valproate in...

Effect of Sodium Valproate on Neuromagnetic Responses to Chromatic Flicker: Implication for Photosensitivity (2006)

K Watanabe, R Sayres, S Shimojo, T Imada, K Nihei

The effect of oral administration of sodium valproate in normal subjects was evaluated using whole-scalp magnetoencephalography, with results compared to the effect of sodium valproate in...

Identification of bovine dendritic cell phenotype from bovine peripheral blood (2006)

Miyazawa, K., Aso, H., Honda, M., Kido, T., Minashima, T., Kanaya, T., ...

Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no...

Discovery of Li2(Pd,Pt)3B superconductors (2006)

Badica, P., Togano, K., Takeya, H., Hirata, K., Awaji, S., Watanabe, K.

Critical temperature Tc of the Li2(Pd1-xPtx)3B was reported to be 7-8K for x=0 and 2.2-2.8K for x=1. In this article we present our preliminary results on behavior of magnetization-temperature curves...

A Comprehensive Search for Gamma-Ray Lines in the First Year of Data from the INTEGRAL Spectrometer (2006)

Teegarden, B. J., Watanabe, K.

We have carried out an extensive search for gamma-ray lines in the first year of public data from the Spectrometer (SPI) on the INTEGRAL mission. INTEGRAL has spent a large fraction of its observing...

Enhancement of JC-B Properties in MoSi2 doped MgB2 tapes (2006)

Zhang, Xianping, Ma, Yanwei, Gao, Zhaoshun, Yu, Zhengguang, Nishijima, G., Watanabe, K.

MoSi2 doped MgB2 tapes with different doping levels were prepared through the in-situ powder-in-tube method using Fe as the sheath material. Effect of MoSi2 doping on the MgB2/Fe tapes was...

Enhanced critical current density of MgB2 superconductor synthesized in high magnetic fields (2006)

Ma, Yanwei, Xu, Aixia, Zhang, Xianping, Li, Xiaohang, Awaji, S., Watanabe, K.

The effect of high magnetic fields on the current carrying properties of both MgB2 bulks and Fe-sheathed tapes was investigated following different thermal sequences. It is found that application of...

Significantly enhanced critical current densities in MgB2 tapes made by a scaleable, nano-carbon addition route (2006)

Ma, Yanwei, Zhang, Xianping, Nishijima, G., Watanabe, K., Bai, Xuedong

Nanocarbon-doped Fe-sheathed MgB2 tapes with different doping levels were prepared by the in situ powder-in-tube method. Compared to the undoped tapes, Jc for all the C-doped samples was enhanced by...

The L2 acquisition of case absorption effects in Japanese and English passives by English and Japanese speakers (2006)

Enomoto, Kayoko, Sakamoto, M., Watanabe, K.

This paper presents findings on the second acquisition (L2) of passives involving case absorption effects by English-speaking learners of Japanese and Japanese-speaking learners of English. Chomsky...

The L2 acquisition of case absorption effects in Japanese and English passives by English and Japanese speakers (2006)

Enomoto, Kayoko, Sakamoto, M., Watanabe, K.

This paper presents findings on the second acquisition (L2) of passives involving case absorption effects by English-speaking learners of Japanese and Japanese-speaking learners of English. Chomsky...

Apoptotic process of porcine intestinal M cells (2005)

Miyazawa, K., Aso, H., Kanaya, T., Kido, T., Minashima, T., Watanabe, K., ...

Membranous (M) cells of the follicle-associated epithelium (FAE) are believed to sample antigens from the gut lumen. However, the origin, differentiation mechanism, and cell death of M cells are...

Solar Neutron Events of October-November 2003 (2005)

Watanabe, K., Gros, M., Stoker, P. H., Kudela, K., Lopate, C., Valdes-Galicia, J. F., ...

During the period when the Sun was intensely active on October-November 2003, two remarkable solar neutron events were observed by the ground-based neutron monitors. On October 28, 2003, in...

Effect of nano-C doping on the in-situ processed MgB2 tapes (2005)

Zhang, Xianping, Ma*, Yanwei, Xu, Aixia, Jiao, Yulei, Xiao, Ling, Awaji, S., ...

The effect of nano-C doping on the microstructure and superconducting properties of Fe-sheathed MgB2 tapes prepared through the in-situ powder-in-tube method was studied. Heat treatment was performed...

Wobble modification deficiency in mutant tRNAs in patients with mitochondrial diseases (2005)

Yasukawa, T., Kirino, Y., Ishii, N., Holt, I.J., Jacobs, H.T., Makifuchi, T., ...

Point mutations in mitochondrial (mt) tRNA genes are associated with a variety of human mitochondrial diseases. We have shown previously that mt tRNALeu(UUR) with a MELAS A3243G mutation and mt...

Comparison of geometric and algebraic multigrid methods in edge-based finite-element analysis (2005)

Watanabe, K., Igarashi, H., Honma, T., 渡邊, 浩太

This paper discusses the comparison between the geometric multigrid (GMG) method and the algebraic multigrid (AMG) method in edge-based finite-element (FE) analysis. The GMG method requires the...

Comparison of geometric and algebraic multigrid methods in edge-based finite-element analysis (2005)

Watanabe, K., Igarashi, H., Honma, T.

This paper discusses the comparison between the geometric multigrid (GMG) method and the algebraic multigrid (AMG) method

Comparison of geometric and algebraic multigrid methods in edge-based finite-element analysis (2005)

Watanabe, K., Igarashi, H., Honma, T.

This paper discusses the comparison between the geometric multigrid (GMG) method and the algebraic multigrid (AMG) method

The optical absorption and photoconductivity spectra of hexagonal boron nitride single crystals (2005)

REMES, Zdenek, Watanabe, K., Taniguchi, T.

Recently, a strong cathodoluminescence at 215 nm (photon energy 5.765 eV) has been reported on pure HPHT hexagonal boron nitride (hBN) single crystals (K. Watanabe et al., Nature Mater. 3, 404...

The optical absorption and photoconductivity spectra of hexagonal boron nitride single crystals (2005)

REMES, Zdenek, Watanabe, K., Taniguchi, T.

Recently, a strong cathodoluminescence at 215 nm (photon energy 5.765 eV) has been reported on pure HPHT hexagonal boron nitride (hBN) single crystals (K. Watanabe et al., Nature Mater. 3, 404...

The optical absorption and photoconductivity spectra of hexagonal boron nitride single crystals (2005)

REMES, Zdenek, NESLADEK, Milos, HAENEN, Ken, Watanabe, K., Taniguchi, T.

Recently, a strong cathodoluminescence at 215 nm (photon energy 5.765 eV) has been reported on pure HPHT hexagonal boron nitride (hBN) single crystals (K. Watanabe et al., Nature Mater. 3, 404...

Influence of microstructure of tungsten on solid state reaction rate with amorphous carbon film (2005)

Hatano, Y, Takamori, M, Nogita, K, Matsuda, K, Ikeno, S, Watanabe, K

Plate-type specimens were cut from three different types of tungsten sheets fabricated under distinct rolling and heat treatment conditions in the directions parallel or perpendicular to the rolling...

Influence of microstructure of tungsten on solid state reaction rate with amorphous carbon film (2005)

Hatano, Y, Takamori, M, Nogita, K, Matsuda, K, Ikeno, S, Watanabe, K

Plate-type specimens were cut from three different types of tungsten sheets fabricated under distinct rolling and heat treatment conditions in the directions parallel or perpendicular to the rolling...

Halothane enhances dopamine metabolism at presynaptic sites in a calcium-independent manner in rat striatum (2005)

Adachi, Y. U., Satomoto, M., Higuchi, H., Watanabe, K., Yamada, S., Kazama, T.

Background. We have previously reported that halothane anaesthesia increases the extracellular concentration of dopamine (DA) metabolites in the rat striatum with no change in DA. Although the...

Halothane enhances dopamine metabolism at presynaptic sites in a calcium-independent manner in rat striatum (2005)

Adachi, Y. U., Satomoto, M., Higuchi, H., Watanabe, K., Yamada, S., Kazama, T.

Background. We have previously reported that halothane anaesthesia increases the extracellular concentration of dopamine (DA) metabolites in the rat striatum with no change in DA. Although the...

Tritium distribution in JET Mark IIA type divertor tiles analysed by BIXS. (2005)

Torikai, Y., Matsuyama, M., Bekris, N., Glugla, M., Coad, P., Naegele, W., ...

16th Internat.Conf.on Plasma Surface Interactions in Controlled Fusion Devices (PSI 2004), Portland, Maine, May 24-28, 2004

Amplification of Quantum Meson Modes in the Late Time of Chiral Phase Transition (2004)

Watanabe, K., Tsue, Y., Nishiyama, S.

It is shown that there exists a possibility of amplification of amplitudes of quantum pion modes with low momenta in the late time of chiral phase transition by using the Gaussian wave functional...

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Inagaki, S., Yokoyama, M., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Inagaki, S., Yokoyama, M., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

INTEGRAL/SPI Limits on Electron-Positron Annihilation Radiation from the Galactic Plane (2004)

Teegarden, B. J., Watanabe, K., Jean, P., Knodlseder, J., Lonjou, V., Roques, J. P., ...

The center of our Galaxy is a known strong source of electron-positron 511-keV annihilation radiation. Thus far, however, there have been no reliable detections of annihilation radiation outside of...

Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis (2004)

Sato,N.S., Suzuki,T., Ueda,T., Watanabe,K., Hirata,R.D.C., Hirata,M.H.

Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and...

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Idei, H., Inagaki, S., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Idei, H., Inagaki, S., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Inagaki, S., Yokoyama, M., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Idei, H., Inagaki, S., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Idei, H., Inagaki, S., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

Extension and its characteristics of ECRH plasma in the LHD (2004)

Kubo, Shin, Shimozuma, T., Yoshimura, Y., Notake, T., Inagaki, S., Yokoyama, M., ...

One of the main objectives of the LHD is to extend the plasma confinement database for helical systems and to demonstrate such extended plasma confinement properties to be sustained in steady state....

NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes (2004)

Cui, XY, Hu, QD, Tekaya, M, Shimoda, Y, Ang, BT, Nie, DY, ...

Neurons and glia in the vertebrate central nervous system arise in temporally distinct, albeit overlapping, phases. Neurons are generated first followed by astrocytes and oligodendrocytes from common...

NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes (2004)

Cui, X. Y., Hu, Q. D., Tekaya, M., Shimoda, Y., Ang, B. T., Nie, D. Y., ...

Neurons and glia in the vertebrate central nervous system arise in temporally distinct, albeit overlapping, phases. Neurons are generated first followed by astrocytes and oligodendrocytes from common...

A phase II study of cisplatin and docetaxel administered as three consecutive weekly infusions for advanced non-small-cell lung cancer in elderly patients (2004)

Ohe, Y., Niho, S., Kakinuma, R., Kubota, K., Ohmatsu, H., Goto, K., ...

Background: To evaluate the efficacy and safety of treatments for advanced non-small-cell lung cancer in elderly patients aged 75 years or older, we conducted a phase II study of cisplatin and...

The profile of gene expression during bovine adipogenesis: Cloning and expression of type XII collagen isoforms (2004)

Aso, H., Tahara, K., Yamasaki, T., Rose, Michael T., Kido, T., Minashima, T., ...

The X International Symposium on Ruminant Physiology, Copenhagen, Denmark, 2004 (Session 4: Nutritional Regulation of Gene Expression).

Competition between hidden order and antiferromagnetism in URu_2Si_2 under uniaxial stress studied by neutron scattering (2003)

Yokoyama, M., Amitsuka, H., Tenya, K., Watanabe, K., Kawarazaki, S., Yoshizawa, H., ...

We have performed elastic neutron scattering experiments under uniaxial stress sigma applied along the tetragonal [100], [110] and [001] directions for the heavy electron compound URu2Si2. We found...

Constraining the Cosmic Star Formation Rate with the MeV Background (2003)

Watanabe, K., Hartmann, D. H., Leising, M. D.

The Cosmic Gamma-ray Background (CGB) in the MeV regime has been measured with COMPTEL and SMM. The origin of the CGB in this energy regime is believed to be dominated by gamma-rays from Type Ia...

The Data Acquisition System Based on PMC Bus (2003)

Igarashi, Y., Fujii, H., Higuchi, T., Ikeno, M., Inoue, E., Itoh, R., ...

High energy physics experiments in KEK/Japan rush into over KHz trigger stage. Thus, we need a successor of the data acquisition(DAQ) system that replaces the CAMAC or FASTBUS systems. To meet these...

Development of a PCI Based Data Acquisition Platform for High Intensity Accelerator Experiments (2003)

Higuchi, T., Fujii, H., Ikeno, M., Igarashi, Y., Inoue, E., Itoh, R., ...

Data logging at an upgraded KEKB accelerator or the J-PARC facility, currently under commission, requires a high density data acquisition platform with integrated data reduction CPUs. To follow...

Applied voltage dependence of nano-oxidation of ferromagnetic thin films using atomic force microscope (2003)

Takemura, Y., Kidaka, S., Watanabe, K., Nasu, Y., Yamada, T., Shirakashi, J., ...

Nanodots of Ni, CoFe, and Cr oxide were fabricated by the nano-oxidation technique using atomic force microscope. The dot size was controlled from 40 to 200 nm by changing the pulse voltage applied...

Convergence of multigrid method for edge-based finite-element method (2003)

Watanabe, K., Igarashi, H., Honma, T., 渡邊, 浩太

This paper discusses robustness of the multigrid (MG) method against distortion of finite elements. The convergence of MG method becomes considerably worse as the finite elements become flat. It is...

Convergence of multigrid method for edge-based finite-element method (2003)

Watanabe, K., Igarashi, H., Honma, T.

This paper discusses robustness of the multigrid (MG) method against distortion of finite elements. The convergence of MG method becomes considerably worse as the finite elements become flat. It is...

Convergence of multigrid method for edge-based finite-element method (2003)

Watanabe, K., Igarashi, H., Honma, T.

This paper discusses robustness of the multigrid (MG) method against distortion of finite elements. The convergence of MG method becomes considerably worse as the finite elements become flat. It is...

Solar Neutron Event in Association with a Large Solar Flare on November 24, 2000 (2003)

Watanabe, K., Muraki, Y., Matsubara, Y., Murakami, K., Sako, T., Tsuchiya, H., ...

Solar neutrons have been detected using the neutron monitor located at Mt. Chacaltaya, Bolivia, in association with a large solar flare on November 24, 2000. This is the first detection of solar...

F3/contactin acts as a functional ligand for notch during oligodendrocyte maturation (2003)

Hu, QD, Ang, BT, Karsak, M, Hu, WP, Cui, XY, Duka, T, ...

Axon-derived molecules are temporally and spatially required as positive or negative signals to coordinate oligodendrocyte differentiation. Increasing evidence suggests that, in addition to the...

F3/contactin acts as a functional ligand for notch during oligodendrocyte maturation (2003)

Hu, Q. D., Ang, B. T., Karsak, M., Hu, W. P., Cui, X. Y., Duka, T., ...

Axon-derived molecules are temporally and spatially required as positive or negative signals to coordinate oligodendrocyte differentiation. Increasing evidence suggests that, in addition to the...

Non-destructive tritium measurements of Mk IIA divertor tile by BIXS. (2003)

Matsuyama, M., Bekris, N., Glugla, M., Noda, N., Philipps, V., Watanabe, K.

Proc. of the 15th Internat.Conf.on Plasma Surface Interactions in Controlled Fusion Devices (PSI-15), Gifu, J, May 27-31, 2002

Picosecond Nonlinear Relaxation of Photoinjected Carriers in a Single GaAs/AlGaAs Quantum Dot (2002)

Kuroda, T., Sanguinetti, S., Gurioli, M., Watanabe, K., Minami, F., Koguchi, N.

Photoemission from a single self-organized GaAs/AlGaAs quantum dot (QD) is temporally resolved with picosecond time resolution. The emission spectra consisting of the multiexciton structures are...

Nonequilibrium Antiferromagnetic State in the Heavy Electron Compound URu_2Si_2 (2002)

Yokoyama, M., Nozaki, J., Amitsuka, H., Watanabe, K., Kawarazaki, S., Yoshizawa, H., ...

We have investigated the nature of the antiferromagnetic (AF) phase induced by uniaxial stress sigma in URu2Si2, by performing elastic neutron scattering measurements up to 0.4 GPa. We have found...

Gamma-Ray Bursts and Cosmic Radiation Backgrounds (2002)

Hartmann, D. H., Kneiske, T. M., Mannheim, K., Watanabe, K.

GRBs contribute to the evolving cosmic radiation field. We discuss the contribution of GRBs to the high-energy background, and the effect of pair creation off low-energy photons on their observable...

Radiation Distribution Sensing With Normal Optical Fiber (2001)

Naka, R., Watanabe, K., Kawarabayashi, J., Uritani, A., Iguchi, T., Hayashi, N., ...

The purpose of this study is to develop a radiation distribution monitor using a normal plastic optical fiber. The monitor has a long operating length and can obtain continuous radiation...

Effects of Uniaxial Stress on Antiferromagnetic Moment in the Heavy Electron Compound URu_2Si_2 (2001)

Yokoyama, M., Amitsuka, H., Watanabe, K., Kawarazaki, S., Yoshizawa, H., Mydosh, J. A.

We have performed the elastic neutron scattering experiments under uniaxial stress \sigma along the tetragonal [100], [110] and [001] directions for URu2Si2. For \sigma // [100] and [110], the...

Contributions of GRBs and Cen A-like Radio Galaxies to the Cosmic Gamma-ray Background (2001)

Watanabe, K., Hartmann, D. H.

The contribution to the cosmic diffuse gamma-ray background (CGB) from Gamma Ray Bursts (GRBs) is studied in the 40 keV - 2 MeV regime. We use High Energy Resolution (HER) data from the Burst And...

MHD characteristics in the high beta regime of the Large Helical Device (2001)

Sakakibara, S., Yamada, H., Watanabe, K., Narushima, Y., Toi, K., Ohdachi, S., ...

The highest volume averaged beta values beta (t) of 2.2% at B-t = 0.75 T (gas puff) and 2.4% at D-t = 1.3 T (pellet) in helical devices have been achieved in LHD. The beta (t) dependence of MHD...

Halothane potentiates the effect of methamphetamine and nomifensine on extracellular dopamine levels in rat striatum: a microdialysis study (2001)

Adachi, Y. U., Watanabe, K., Satoh, T., Vizi, E. S.

Brain microdialysis was used to study the in vivo release and metabolism of dopamine (DA) in the rat striatum during halothane anaesthesia. Concentrations were measured in microdialysates collected...

Antimicrobial resistance and serotype distribution of Streptococcus pneumoniae strains causing childhood infections in Bangladesh, 1993 to 1997 (1999)

Saha, Samir K., Rikitomi, N., Ruhulamin, M., Masaki, H., Hanif, M., Islam, Maksuda, ...

Three hundred sixty-two Streptococcus pneumoniae strains were isolated from children under 5 years of age at Dhaka Shishu (Children) Hospital from 1993 to 1997. The strains were isolated from blood...

Studying the High-Energy Gamma-Ray Sky with Glast (1999)

Kamae, T., Ohsugi, T., Thompson, D. J., Watanabe, K.

Building on the success of the Energetic Gamma Ray Experiment Telescope (EGRET) on the Compton Gamma Ray Observatory, the Gamma-ray Large Area Space Telescope (GLAST) will make a major step in the...

GPC4, the gene for human K-glypican, flanks GPC3 on xq26: deletion of the GPC3-GPC4 gene cluster in one family with Simpson-Golabi-Behmel syndrome (1998)

Watanabe, K, Yamaguchi, Y

The glypicans constitute a growing family of cell surface heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. Recently, deletions and...

ABSORPTION COEFFICIENTS OF 02 IN THE VACUUM ULTRAVIOLET (1998)

MATSUNAGA,F.M., WATANABE,K.

With a resolution of 0.2 A the absorption coefficients of O2 wereobtained for about 600 wavelengths in the region 840-1100 A and also at 584.3 A. This method resolved band structures which were not...

Permanent Magnetic Properties in Fe-Pt-Nb System Alloys. (1998)

Watanabe, K.

Permanent magnet properties and temperature dependences of Fe-Pt-Nb ternary alloys were studied using a recording flux meter, a vibrating sample magnetometer and an X-ray diffractometer; permanent...

Nb3Sn Multifilamentary Wires With CuNb Reinforcing Stabilizer. (1998)

Watanabe, K., Hoshi, A., Awaji, S., Katagiri, K., Noto, K.

Newly developed was a bronze processed multifilamentary nb3sn superconducting wire, cunb/nb3sn, with cunb reinforcing stabilizer, which exhibited a residual resistance ratio of 20 and a...

Research and Development of Ceramic Turbine Wheels. (1998)

Watanabe, K., Masuda, M., Ozawa, T., Matsui, M., Matsuhiro, K.

The binding strength of specimens of various sizes, some of which were cut from a turbine wheel, was compared with predictions using Weibull statistical theory. Thhe stress distribution of a ceramic...

The Diffuse Gamma-Ray Background from Supernovae (1998)

Watanabe, K., Hartmann, D. H., Leising, M. D.

The Cosmic Gamma-ray Background (CGB) in the MeV region is believed to be due to photons from radioactivity produced in SNe throughout the history of galaxies in the universe. In particular,...

Constraints on Association of Single-pulse Gamma-ray Bursts and Supernovae (1998)

Norris, J. P., Bonnell, J. T., Watanabe, K.

We explore the hypothesis, similar to one recently suggested by Bloom and colleagues, that some nearby supernovae are associated with smooth, single-pulse gamma-ray bursts, possibly having no...

Studies on the Pathogenicity of Anaerobes, Especially Prevotella bivia, in a Rat Pyometra Model (1998)

H. Mikamo, K. Kawazoe, K. Izumi, K. Watanabe, K. Ueno, T. Tamaya

Objective: Prevotella bivia is one of the anaerobic bacteria that resides in the flora of the female genital tract. We studied the pathogenicity of P. bivia in a rat pyometra model.Methods: The...

Dystrophin negative skeletal and myocardial muscle cells in a carrier of Duchenne's muscular dystrophy (1993)

WATANABE, K., IZUMI, T., NATSUI, M., MATSUBARA, N., MIYAKITA, Y., KOYAMA, S., ...

A 47-year-old woman whose elder son had typical Duchenne's muscular dystrophy (DMD) was diagnosed as the manifesting carrier of the disease. She had developed congestive heart failure but had no...

A Nationwide Survey of Congenital Hydrocephalus in Japan -- Estimated Prevalence and Incidence (1991)

Tamakoshi, A., Sasaki, R., Hamajima, N., Aoki, K., Suzuki, S., Yanagawa, H., ...

A nationwide survey of congenital hydrocephalus, including secondary hydrocephalus occurring within one year after birth, was carried out in 1988 using two questionnaires. The first questionnaire,...

Magnetocardiographic studies of ventricular repolarization in old inferior myocardial infarction (1989)

NOMURA, M., NAKAYA, Y., FUJINO, K., ISHIHARA, S., KATAYAMA, M., TAKEUCHI, A., ...

Isomagnetic maps of 50 normal subjects (control group) and 23 patients with old inferior myocardial infarction (IMI group) were recorded in order to analyse T wave abnormalities in inferior...

METAL-INSULATOR TRANSITION IN PYRITE TYPE NiS2-xSex SYSTEM (1988)

Miyadai, T., Tazuke, Y., Kinouchi, S., Nishioka, T., Sudo, S., Miyako, Y., ...

Specific heat and Raman scattering were measured on sintered samples with x around 0.5, which show a sharp metal-to-insulator transition (MIT) with increasing temperature. Specific heat below MIT can...

Study on fundamental defects and their effect on GaAs device properties (1988)

Miyazawa, S., Watanabe, K., Osaka, J., Ikuta, K.

Our understanding of the causal relationship between material parameters and GaAs FET threshold voltage is briefly presented from the viewpoint of point defect reactions. Primarily, dislocations in...

THEORY OF FIELD ADSORPTION (1988)

Kreuzer, H., Watanabe, K.

We discuss the explicit construction of diabatic states which form the basis to study the kinetics of field desorption, ionization and eventually field-induced surface chemistry. We indicate the...

METAL-INSULATOR TRANSITION IN PYRITE TYPE NiS2-xSex SYSTEM (1988)

Miyadai, T., Tazuke, Y., Kinouchi, S., Nishioka, T., Sudo, S., Miyako, Y., ...

Specific heat and Raman scattering were measured on sintered samples with x around 0.5, which show a sharp metal-to-insulator transition (MIT) with increasing temperature. Specific heat below MIT can...

Study on fundamental defects and their effect on GaAs device properties (1988)

Miyazawa, S., Watanabe, K., Osaka, J., Ikuta, K.

Our understanding of the causal relationship between material parameters and GaAs FET threshold voltage is briefly presented from the viewpoint of point defect reactions. Primarily, dislocations in...

THEORY OF FIELD ADSORPTION (1988)

Kreuzer, H., Watanabe, K.

We discuss the explicit construction of diabatic states which form the basis to study the kinetics of field desorption, ionization and eventually field-induced surface chemistry. We indicate the...

METAL-INSULATOR TRANSITION IN PYRITE TYPE NiS2-xSex SYSTEM (1988)

Miyadai, T., Tazuke, Y., Kinouchi, S., Nishioka, T., Sudo, S., Miyako, Y., ...

Specific heat and Raman scattering were measured on sintered samples with x around 0.5, which show a sharp metal-to-insulator transition (MIT) with increasing temperature. Specific heat below MIT can...

Study on fundamental defects and their effect on GaAs device properties (1988)

Miyazawa, S., Watanabe, K., Osaka, J., Ikuta, K.

Our understanding of the causal relationship between material parameters and GaAs FET threshold voltage is briefly presented from the viewpoint of point defect reactions. Primarily, dislocations in...

THEORY OF FIELD ADSORPTION (1988)

Kreuzer, H., Watanabe, K.

We discuss the explicit construction of diabatic states which form the basis to study the kinetics of field desorption, ionization and eventually field-induced surface chemistry. We indicate the...

Electret Dosemeters for Tritium Beta Rays (1986)

Miki, T., Ikeya, M., Matsuyama, M., Watanabe, K.

The decrease of the electret charge by exposure to gaseous tritium was studied in order to develop an electret personnel dosemeter for tritium. The electret charge decreases in proportion to the...

A 50 mm BORE, 13 TESLA SUPERCONDUCTING MAGNET EMPLOYING A PREREACTED MULTIFILAMENTARY Nb3Sn CONDUCTOR (1984)

Noto, K., Watanabe, K., Muto, Y., Ikeda, M., Tanaka, Y., Inoue, I., ...

Following the construction of two hybrid magnets, we intend to build up a third hybrid magnet which will produce about 30 T. A prereacted multifilamentary Nb3Sn conductor will be employed for the 12...

HYBRID MAGNET PROJECT AT TOHOKU UNIVERSITY (1984)

Nakagawa, Y., Noto, K., Hoshi, A., Miura, S., Watanabe, K., Muto, Y.

The construction of three hybrid magnets has been conceived in this project. The smallest one now in operation is composed of a non-cryostable NbTi coil weighing 270 kg and a Bitter coil with a power...

A 50 mm BORE, 13 TESLA SUPERCONDUCTING MAGNET EMPLOYING A PREREACTED MULTIFILAMENTARY Nb3Sn CONDUCTOR (1984)

Noto, K., Watanabe, K., Muto, Y., Ikeda, M., Tanaka, Y., Inoue, I., ...

Following the construction of two hybrid magnets, we intend to build up a third hybrid magnet which will produce about 30 T. A prereacted multifilamentary Nb3Sn conductor will be employed for the 12...

HYBRID MAGNET PROJECT AT TOHOKU UNIVERSITY (1984)

Nakagawa, Y., Noto, K., Hoshi, A., Miura, S., Watanabe, K., Muto, Y.

The construction of three hybrid magnets has been conceived in this project. The smallest one now in operation is composed of a non-cryostable NbTi coil weighing 270 kg and a Bitter coil with a power...

A 50 mm BORE, 13 TESLA SUPERCONDUCTING MAGNET EMPLOYING A PREREACTED MULTIFILAMENTARY Nb3Sn CONDUCTOR (1984)

Noto, K., Watanabe, K., Muto, Y., Ikeda, M., Tanaka, Y., Inoue, I., ...

Following the construction of two hybrid magnets, we intend to build up a third hybrid magnet which will produce about 30 T. A prereacted multifilamentary Nb3Sn conductor will be employed for the 12...

HYBRID MAGNET PROJECT AT TOHOKU UNIVERSITY (1984)

Nakagawa, Y., Noto, K., Hoshi, A., Miura, S., Watanabe, K., Muto, Y.

The construction of three hybrid magnets has been conceived in this project. The smallest one now in operation is composed of a non-cryostable NbTi coil weighing 270 kg and a Bitter coil with a power...

BEHAVIOUR OF WELDED PART OF Ti-Ni SHAPE MEMORY ALLOY (1982)

Nishikawa, M., Tanaka, H., Kohda, M., Nagaura, T., Watanabe, K.

Thin wires of Ti-Ni shape memory alloy are welded without fusion or cast zone by the resistance butt welding. The weld current is fed to the butt welded parts with little offset during the time...

BEHAVIOUR OF WELDED PART OF Ti-Ni SHAPE MEMORY ALLOY (1982)

Nishikawa, M., Tanaka, H., Kohda, M., Nagaura, T., Watanabe, K.

Thin wires of Ti-Ni shape memory alloy are welded without fusion or cast zone by the resistance butt welding. The weld current is fed to the butt welded parts with little offset during the time...

BEHAVIOUR OF WELDED PART OF Ti-Ni SHAPE MEMORY ALLOY (1982)

Nishikawa, M., Tanaka, H., Kohda, M., Nagaura, T., Watanabe, K.

Thin wires of Ti-Ni shape memory alloy are welded without fusion or cast zone by the resistance butt welding. The weld current is fed to the butt welded parts with little offset during the time...

AN H2 (D2)/F2 CHEMICAL LASER INITIATED WITH A NOVEL TEFLON SURFACE SPARK UV FLASH (1980)

Watanabe, K., Sato, Y., Lee, C., Obara, M., Fujioka, T.

The performance characteristics and the optimization parameters of a newly developed HF (DF) laser initiated by a novel surface spark UV flash using Teflon are presented. We have obtained an output...

AN H2 (D2)/F2 CHEMICAL LASER INITIATED WITH A NOVEL TEFLON SURFACE SPARK UV FLASH (1980)

Watanabe, K., Sato, Y., Lee, C., Obara, M., Fujioka, T.

The performance characteristics and the optimization parameters of a newly developed HF (DF) laser initiated by a novel surface spark UV flash using Teflon are presented. We have obtained an output...

AN H2 (D2)/F2 CHEMICAL LASER INITIATED WITH A NOVEL TEFLON SURFACE SPARK UV FLASH (1980)

Watanabe, K., Sato, Y., Lee, C., Obara, M., Fujioka, T.

The performance characteristics and the optimization parameters of a newly developed HF (DF) laser initiated by a novel surface spark UV flash using Teflon are presented. We have obtained an output...

Proton magnetic resonance spectra of tRNAfMet from Thermus thermophilus (1977)

Kyogoku, Y., Lnubushi, T., Morishima, I., Watanabe, K., Oshima, T., Nishimura, S.

220 MHz proton magnetic resonance spectra of tRNAs in bulk and tRHAfMet from Thermus therwophilus have been measured and compared with those of tRNAs from E. coli. Temperature dependences and...

The Role of C2H4 in anaerobic induction of cocklebur seed germination (1976)

Esashi, Y., Okazaki, M., Watanabe, K.

Non-dormant small cocklebur seeds (Xanthium pennsylvanicum Wallr.) are potentiated to germinate, if they are subjected to anaerobic condition for certain time periods after being sufficiently...

PHOTOIONIZATION OF SEVERAL MOLECULES (1954)

WATANABE, K.

Author Institution: Geophysics Research Directorate, Air Force Cambridge Research Center

Protective immune responses induced by secretion of a chimeric soluble protein from a recombinant Mycobacterium bovis bacillus Calmette-Guérin vector candidate vaccine for human immunodeficiency virus type 1 in small animals.

Honda, M, Matsuo, K, Nakasone, T, Okamoto, Y, Yoshizaki, H, Kitamura, K, ...

A recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) vector-based vaccine that secretes the V3 principal neutralizing epitope of human immunodeficiency virus (HIV) could induce immune...

One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts.

Melchers, G, Mohri, Y, Watanabe, K, Wakabayashi, S, Harada, K

Mesophyll protoplasts of Lycopersicon esculentum were treated with iodoacetamide to inactivate mitochondria, and protoplasts of Solanum acaule and Solanum tuberosum were irradiated with gamma- or...

Definition of a physiologic aging autoantigen by using synthetic peptides of membrane protein band 3: localization of the active antigenic sites.

Kay, M M, Marchalonis, J J, Hughes, J, Watanabe, K, Schluter, S F

Senescent cell antigen (SCA), an aging antigen, is a protein that appears on old cells and marks them for removal by the immune system in mammals. It is derived from band 3, a ubiquitous membrane...

Antimicrobial Resistance and Serotype Distribution of Streptococcus pneumoniae Strains Causing Childhood Infections in Bangladesh, 1993 to 1997

Saha, Samir K., Rikitomi, N., Ruhulamin, M., Masaki, H., Hanif, M., Islam, Maksuda, ...

Three hundred sixty-two Streptococcus pneumoniae strains were isolated from children under 5 years of age at Dhaka Shishu (Children) Hospital from 1993 to 1997. The strains were isolated from blood...

Preparation of biologically active Ascaris suum mitochondrial tRNAMet with a TV-replacement loop by ligation of chemically synthesized RNA fragments.

Ohtsuki, T, Kawai, G, Watanabe, Y, Kita, K, Nishikawa, K, Watanabe, K

Ascaris suum mitochondrial tRNA Met lacking the entire T stem was prepared by enzymatic ligation of two chemically synthesized RNA fragments. The synthetic tRNA could be charged with methionine by...

An RNA fragment consisting of the P7 and P9.0 stems and the 3'-terminal guanosine of the Tetrahymena group I intron.

Watanabe, S, Kawai, G, Muto, Y, Watanabe, K, Inoue, T, Yokoyama, S

On the basis of the nucleotide sequence of Tetrahymena group I intron, we constructed a 31 residue RNA that has the P7 stem and the 3'-terminal guanosine residue (3'-G) with a putative stem-loop...

RNA editing in the acceptor stem of squid mitochondrial tRNA(Tyr).

Tomita, K, Ueda, T, Watanabe, K

In squid (Loligo bleekeri) mitochondria, the two 3'-terminal nucleotides (G72-G73) of the tRNA(Tyr) gene overlap with the two 5'-terminal nucleotides (G1-G2) of the downstream tRNA(Cys) gene. To...

Expression of bovine mitochondrial tRNASer GCU derivatives in Escherichia coli.

Hayashi, I, Kawai, G, Watanabe, K

By replacing a stretch of five A-U base pairs in the acceptor stem with G-C pairs, mitochondrial tRNA-SerGCU lacking a D arm could be expressed in Escherichia coli cells in considerable amounts. The...

The presence of pseudouridine in the anticodon alters the genetic code: a possible mechanism for assignment of the AAA lysine codon as asparagine in echinoderm mitochondria.

Tomita, K, Ueda, T, Watanabe, K

It has been inferred from DNA sequence analyses that in echinoderm mitochondria not only the usual asparagine codons AAU and AAC, but also the usual lysine codon AAA, are translated as asparagine by...

An extra tRNAGly(U*CU) found in ascidian mitochondria responsible for decoding non-universal codons AGA/AGG as glycine.

Kondow, A, Suzuki, T, Yokobori, S, Ueda, T, Watanabe, K

Amino acid assignments of metazoan mitochondrial codons AGA/AGG are known to vary among animal species; arginine in Cnidaria, serine in invertebrates and stop in vertebrates. We recently found that...

Codon reading patterns in Drosophila melanogaster mitochondria based on their tRNA sequences: a unique wobble rule in animal mitochondria.

Tomita, K, Ueda, T, Ishiwa, S, Crain, P F, McCloskey, J A, Watanabe, K

Mitochondrial (mt) tRNA(Trp), tRNA(Ile), tRNA(Met), tRNA(Ser)GCU, tRNA(Asn)and tRNA(Lys)were purified from Drosophila melanogaster (fruit fly) and their nucleotide sequences were determined....

NP-06: a novel anti-human immunodeficiency virus polypeptide produced by a Streptomyces species.

Chokekijchai, S, Kojima, E, Anderson, S, Nomizu, M, Tanaka, M, Machida, M, ...

From an extract of a Streptomyces culture, we identified and purified a novel compound, NP-06, which is active against human immunodeficiency virus (HIV) in vitro. Analyses indicate that NP-06 is a...

In vitro activities of 10 antimicrobial agents against bacterial vaginosis-associated anaerobic isolates from pregnant Japanese and Thai women.

Puapermpoonsiri, S, Watanabe, K, Kato, N, Ueno, K

The in vitro activities of 10 antimicrobial agents against 159 bacterial vaginosis-associated anaerobic isolates from pregnant Japanese and Thai women were determined. Clindamycin, imipenem,...

Analysis of the critical sites for protein thermostabilization by proline substitution in oligo-1,6-glucosidase from Bacillus coagulans ATCC 7050 and the evolutionary consideration of proline residues.

Watanabe, K, Kitamura, K, Suzuki, Y

To identify the critical sites for protein thermostabilization by proline substitution, the gene for oligo-1,6- glucosidase from a thermophilic Bacillus coagulans strain, ATCC 7050, was cloned as a...

Identification of a functionally important population in phenol-digesting activated sludge with antisera raised against isolated bacterial strains.

Watanabe, K, Hino, S

Antisera were raised against nine strains which had been isolated from phenol-acclimated oil refinery activated sludge. Although several antisera reacted significantly with the activated sludge...

A monoclonal antibody reactive with a common epitope of Moraxella (Branhamella) catarrhalis lipopolysaccharides.

Oishi, K, Tanaka, H, Sonoda, F, Borann, S, Ahmed, K, Utsunomiya, Y, ...

A hybrid cell line producing a monoclonal antibody (MAb) against Moraxella (Branhamella) catarrhalis lipopolysaccharide (LPS) was established. The specificity of the MAb 1B12 to purified rough LPSs...

Macrolide accumulation by Bacteroides fragilis ATCC 25285.

Muto, Y, Bandoh, K, Watanabe, K, Katoh, N, Ueno, K

The accumulation of macrolide antibiotics in Bacteroides fragilis ATCC 25285 was increased in the order erythromycin, josamycin, and rokitamycin, depending on hydrophobicity. The half-times of efflux...

Effects of Clostridium difficile toxin A and toxin B on phospholipase D activation in human promyelocytic leukemic HL60 cells.

Ohguchi, K, Banno, Y, Nakashima, S, Kato, N, Watanabe, K, Lyerly, D M, ...

The possible involvement of Rho family GTP-binding proteins in the regulation of phospholipase D (PLD) activity has recently been demonstrated. In the present study, to further examine the role of...

Emergence of fluoroquinolone resistance in Bacteroides fragilis accompanied by resistance to beta-lactam antibiotics.

Kato, N, Miyauchi, M, Muto, Y, Watanabe, K, Ueno, K

The in vitro susceptibilities of a group of 93 isolates of Bacteroides fragilis collected from 1983 to 1984 to six fluoroquinolones were compared with those of a group of 93 isolates collected from...

Physiological and biochemical analysis of the effects of alkaline phosphatase overproduction in Escherichia coli.

Kadokura, H, Watanabe, K, Tsuneizumi, K, Yoda, K, Yamasaki, M

Overexpression of the Escherichia coli phoA gene, coding for alkaline phosphatase (PhoA), on multicopy plasmids caused a severe defect in the precursor processing (secretion) of PhoA, beta-lactamase,...

Susceptibilities of anaerobic bacteria to N-formimidoyl thienamycin (MK0787) and to other antibiotics.

Kesado, T, Watanabe, K, Asahi, Y, Isono, M, Ueno, K

The susceptibilities of 462 clinical anaerobic bacterial isolates to N-formimidoyl thienamycin and 16 other currently available and investigational antibiotics were determined by the agar dilution...

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis.

Kesado, T, Watanabe, K, Asahi, Y, Isono, M, Ueno, K

The in vitro antibacterial activities of the newly developed 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against 67 clinical isolates of Bacteroides fragilis and...

Construction and characterization of a fimA mutant of Porphyromonas gingivalis.

Hamada, N, Watanabe, K, Sasakawa, C, Yoshikawa, M, Yoshimura, F, Umemoto, T

Although fimbriae of Porphyromonas gingivalis have been implicated as playing a major role in adherence to gingival tissue surfaces, no conclusive genetic evidence has yet been obtained. The fimA...

Therapeutic effects of a human antiflagella monoclonal antibody in a neutropenic murine model of Pseudomonas aeruginosa pneumonia.

Oishi, K, Sonoda, F, Iwagaki, A, Ponglertnapagorn, P, Watanabe, K, Nagatake, T, ...

Human immunoglobulin G1 (IgG1) monoclonal antibodies (MAbs) reactive with type-specific Pseudomonas aeruginosa lipopolysaccharide (LPS) and flagella were compared for their protective activities...

In vitro activity of FK037, a new parenteral cephalosporin, against anaerobic bacteria.

Kato, N, Kato, H, Tanaka, Y, Bando, K, Watanabe, K, Ueno, K

The activity of FK037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. A fastidious...

Mechanism for inhibition of influenza virus RNA polymerase activity by matrix protein.

Watanabe, K, Handa, H, Mizumoto, K, Nagata, K

Influenza virus M1 protein has been shown to inhibit the transcription catalyzed by viral ribonucleoprotein complexes isolated from virions. Here, this inhibition mechanism was studied with the...

Use of Loop-Mediated Isothermal Amplification of the IS900 Sequence for Rapid Detection of Cultured Mycobacterium avium subsp. paratuberculosis

Enosawa, M., Kageyama, S., Sawai, K., Watanabe, K., Notomi, T., Onoe, S., ...

We evaluated the usefulness of loop-mediated isothermal amplification (LAMP) in detecting specific gene sequences of Mycobacterium avium subsp. paratuberculosis (MAP). A total of 102 primer sets for...

Identification of dimethyl disulfide-forming bacteria isolated from activated sludge.

Tomita, B, Inoue, H, Chaya, K, Nakamura, A, Hamamura, N, Ueno, K, ...

Twenty-four strains with high dimethyl disulfide (DMDS)-forming ability were isolated from activated sludge and identified to the genus level. These bacteria were classified into four groups (A, B,...

Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.

Watanabe, K, Hayashida, M, Nakashima, Y, Hayashi, S

Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM...

Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius.

Watanabe, K, Iha, H, Ohashi, A, Suzuki, Y

The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a...

Molecular cloning of a gene for indole-3-acetamide hydrolase from Bradyrhizobium japonicum.

Sekine, M, Watanabe, K, Syono, K

A pLAFR1 cosmid genomic library of wild-type Bradyrhizobium japonicum J1063 was constructed. A cosmid clone designated pBjJ4, containing a 26-kilobase (kb) DNA insert, was identified as being able to...

Serotypes of Streptococcus pneumoniae causing invasive childhood infections in Bangladesh, 1992 to 1995.

Saha, S K, Rikitomi, N, Biswas, D, Watanabe, K, Ruhulamin, M, Ahmed, K, ...

One hundred sixty-five invasive Streptococcus pneumoniae strains were isolated from children under five at Dhaka Shishu (Children's) Hospital during the period 1992 to 1995. Ninety-four strains were...

Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains.

Murata, K, Fukuda, Y, Shimosaka, M, Watanabe, K, Saikusa, T, Kimura, A

The gene responsible for the methylglyoxal resistance of Saccharomyces cerevisiae was cloned, and its phenotypic characteristics were investigated. S. cerevisiae cells with the gene could accumulate...

Biochemical properties and purification of metallo-beta-lactamase from Bacteroides fragilis.

Bandoh, K, Muto, Y, Watanabe, K, Katoh, N, Ueno, K

The beta-lactamase from Bacteroides fragilis GAI-30144 hydrolyzed imipenem, oxyiminocephalosporins, cephamycins, and penicillins. Enzyme activity was inhibited by EDTA. Zinc completely reversed...

Level of neutrophil chemotactic factor CINC/gro, a member of the interleukin-8 family, associated with lipopolysaccharide-induced inflammation in rats.

Iida, M, Watanabe, K, Tsurufuji, M, Takaishi, K, Iizuka, Y, Tsurufuji, S

Rat cytokine-induced neutrophil chemoattractant (CINC), which is a counterpart of human gro and belongs to the interleukin-8 family, has been quantified by a new sandwich enzyme-linked immunosorbent...

Antigenic role of stress-induced catalase of Salmonella typhimurium in cell-mediated immunity.

Kagaya, K, Miyakawa, Y, Watanabe, K, Fukazawa, Y

The ability of the H2O2-induced catalase of Salmonella typhimurium to induce cell-mediated immunity against S. typhimurium infection in mice was examined. When exponentially growing cells of S....

Mechanism for candidacidal activity in macrophages activated by recombinant gamma interferon.

Watanabe, K, Kagaya, K, Yamada, T, Fukazawa, Y

Candidacidal activity in macrophages activated by recombinant gamma interferon was examined kinetically in relation to acidification of phagolysosomes. In resident peritoneal macrophages (PMPs) of...

Purification and properties of a 75-kilodalton major protein, an immunodominant surface antigen, from the oral anaerobe Bacteroides gingivalis.

Yoshimura, F, Watanabe, K, Takasawa, T, Kawanami, M, Kato, H

A 75-kilodalton major protein (75K protein) was purified to homogeneity from the cell lysate fraction and the envelope of Bacteroides gingivalis 381. The 75K protein was originally present in the...

Acute joint inflammation in mice after systemic injection of the cell wall, its peptidoglycan, and chemically defined peptidoglycan subunits from various bacteria.

Koga, T, Kakimoto, K, Hirofuji, T, Kotani, S, Ohkuni, H, Watanabe, K, ...

The systemic injection of an aqueous suspension of cell wall or its peptidoglycan (PG)-rich sonicate derived from group A streptococcus and Lactobacillus casei induced acute joint lesions in BALB/c,...

Typing of Clostridium difficile by western immunoblotting with 10 different antisera.

Kato, H, Cavallaro, J J, Kato, N, Bartley, S L, Killgore, G E, Watanabe, K, ...

Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were...

Application of typing by pulsed-field gel electrophoresis to the study of Clostridium difficile in a neonatal intensive care unit.

Kato, H, Kato, N, Watanabe, K, Ueno, K, Ushijima, H, Hashira, S, ...

Pulsed-field gel electrophoresis (PFGE) analysis of restriction pattern polymorphism was applied to type Clostridium difficile isolated from neonates hospitalized in a neonatal intensive care unit,...

Isolation of Clostridium pseudotetanicum from a patient with gas gangrene.

Katoh, N, Watanabe, K, Miki, Y, Muro, T, Ueno, K

Clostridium pseudotetanicum was isolated along with Serratia marcescens, Proteus mirabilis, and Staphylococcus epidermidis from a patient suffering from gas gangrene who had been injured in the right...

Structural similarity of bovine lung prostaglandin F synthase to lens epsilon-crystallin of the European common frog.

Watanabe, K, Fujii, Y, Nakayama, K, Ohkubo, H, Kuramitsu, S, Kagamiyama, H, ...

Cloned cDNA sequences specific for prostaglandin F (PGF) synthase have been isolated from a cDNA library of bovine lung mRNA sequences. Nucleotide-sequence analyses of cloned cDNA inserts have...

Low shear stress can initiate von Willebrand factor-dependent platelet aggregation in patients with type IIB and platelet-type von Willebrand disease.

Murata, M, Fukuyama, M, Satoh, K, Fujimura, Y, Yoshioka, A, Takahashi, H, ...

Platelets exposed to shear stress aggregate in the absence of exogenously added agonists, utilizing distinct platelet membrane receptors and ligands depending upon the level of shear stress applied....

The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress.

Ikeda, Y, Handa, M, Kawano, K, Kamata, T, Murata, M, Araki, Y, ...

Exposure of platelets to shear stress leads to aggregation in the absence of exogenous agonists. We have now found that different adhesive proteins and platelet membrane glycoproteins are involved in...

Human salivary gustin is a potent activator of calmodulin-dependent brain phosphodiesterase.

Law, J S, Nelson, N, Watanabe, K, Henkin, R I

Human salivary gustin stimulated activity of brain calmodulin-dependent cyclic nucleotide phosphodiesterase (cAMP PDEase; 3',5'-cyclic-nucleotide phosphodiesterase, EC 3.1.4.17) in a dose-dependent...

Unusual anticodon loop structure found in E.coli lysine tRNA.

Watanabe, K, Hayashi, N, Oyama, A, Nishikawa, K, Ueda, T, Miura, K

Although both tRNA(Lys) and tRNA(Glu) of E. coli possess similar anticodon loop sequences, with the same hypermodified nucleoside 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the first position of...

Characterization of serine and leucine tRNAs in an asporogenic yeast Candida cylindracea and evolutionary implications of genes for tRNA(Ser)CAG responsible for translation of a non-universal genetic code.

Suzuki, T, Ueda, T, Yokogawa, T, Nishikawa, K, Watanabe, K

Five serine and three leucine isoaceptor tRNAs were purified from the asporogenic yeast Candida cylindracea, in which codon CUG is translated as serine instead of leucine, and their primary...

Most compact hairpin-turn structure exerted by a short DNA fragment, d(GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat.

Hirao, I, Kawai, G, Yoshizawa, S, Nishimura, Y, Ishido, Y, Watanabe, K, ...

The three-dimensional structure of a short DNA fragment, d(GCGAAGC) exhibiting an extraordinarily stable hairpin structure was determined by nuclear magnetic resonance spectroscopy. Two possible...

Non-universal decoding of the leucine codon CUG in several Candida species.

Ohama, T, Suzuki, T, Mori, M, Osawa, S, Ueda, T, Watanabe, K, ...

It has been reported that CUG, a universal leucine codon, is read as serine in an asporogenic yeast, Candida cylindracea. The distribution of this non-universal genetic code in various yeast species...

The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.

Watanabe, K, Yamano, Y, Murata, K, Kimura, A

The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted...

Nucleotide sequence of the gene responsible for D-xylose uptake in Escherichia coli.

Kurose, N, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature....

Capacity of recombinant gamma interferon to activate macrophages for Salmonella-killing activity.

Kagaya, K, Watanabe, K, Fukazawa, Y

The ability of recombinant gamma interferon (rIFN-gamma) to activate macrophages for Salmonella-killing activity was kinetically examined in relation to phagosome-lysosome fusion and H2O2 generation....

Extraordinary stable structure of short single-stranded DNA fragments containing a specific base sequence: d(GCGAAAGC).

Hirao, I, Nishimura, Y, Naraoka, T, Watanabe, K, Arata, Y, Miura, K

Short single-stranded DNA fragments carrying a GCGAAAGC sequence were found to move unexpectedly faster than other fragments of the same length in electrophoresis on a polyacrylamide gel containing a...

Purification and characterization of two serine isoacceptor tRNAs from bovine mitochondria by using a hybridization assay method.

Yokogawa, T, Kumazawa, Y, Miura, K, Watanabe, K

For large scale preparation of mitochondrial tRNAs, a new hybridization assay method using synthetic oligodeoxyribonucleotide probes (16-17mer) complementary to individual tRNA sequences was...

Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.

Ohta, Y, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 1,243 bp in length coding for N-acetylneuraminate lyase (N-acetylneuraminate pyruvate lyase; NPL) of Escherichia coli has been determined. Nucleotide...

Graphic display of nucleic acid structure by a microcomputer.

Watanabe, K, Yasukawa, K, Iso, K

A microcomputer program for the display of a three dimensional structure of a nucleic acid and a protein has been developed. This program can generate a wire model and a ball and stick model on a...

Stereospecific conversion of prostaglandin D2 to (5Z,13E)-(15S)-9 alpha-11 beta,15-trihydroxyprosta-5,13-dien-1-oic acid (9 alpha,11 beta-prostaglandin F2) and of prostaglandin H2 to prostaglandin F2 alpha by bovine lung prostaglandin F synthase.

Watanabe, K, Iguchi, Y, Iguchi, S, Arai, Y, Hayaishi, O, Roberts, L J

A prostaglandin F (PGF) synthase was recently purified from bovine lung that catalyzed the reduction of both PGH2 and PGD2 but at different active sites on the enzyme. In view of the recent finding...

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.

Davanloo, P, Sprinzl, M, Watanabe, K, Albani, M, Kersten, H

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from...

A novel cloverleaf structure found in mammalian mitochondrial tRNA(Ser) (UCN).

Yokogawa, T, Watanabe, Y, Kumazawa, Y, Ueda, T, Hirao, I, Miura, K, ...

Bovine mitochondrial tRNA(Ser) (UCN) has been thought to have two U-U mismatches at the top of the acceptor stem, as inferred from its gene sequence. However, this unusual structure has not been...

Higher-order structure of bovine mitochondrial tRNA(SerUGA): chemical modification and computer modeling.

Watanabe, Y, Kawai, G, Yokogawa, T, Hayashi, N, Kumazawa, Y, Ueda, T, ...

On the basis of enzymatic probing and phylogenetic comparison, we have previously proposed that mammalian mitochondrial tRNA(sSer) (anticodon UGA) possess a slightly altered cloverleaf structure in...

Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro.

Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Shimizu, M

The discrimination mechanism between tRNA(Ser) and tRNA(Tyr) was studied using various in vitro transcripts of E. coli tRNATyr variants. The insertion of only two nucleotides into the variable stem...

Phosphorothioate-containing RNAs show mRNA activity in the prokaryotic translation systems in vitro.

Ueda, T, Tohda, H, Chikazumi, N, Eckstein, F, Watanabe, K

Phosphorothioate-containing RNAs were generated by transcription of coliphage T7 DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) and T7 RNA polymerase. RNAs in which a...

Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCGAAAGC) and their effect on DNA sequencing.

Hirao, I, Nishimura, Y, Tagawa, Y, Watanabe, K, Miura, K

A small DNA fragment having a characteristic sequence d(GCGAAAGC) has been shown to form an extraordinarily stable mini-hairpin structure and to have an unusually rapid mobility in polyacrylamide gel...

Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.

Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Miura, K, Shimizu, M

All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate the role of this unique structure in aminoacylation, we constructed in...

Proton magnetic resonance spectra of tRNA-Met-f from Thermus thermophilus.

Kyogoku, Y, Inubushi, T, Morishima, I, Watanabe, K, Oshima, T, Nishimura, S

220 MHz proton magnetic resonance spectra of tRNAs in bulk and tRNA-Met-f from Thermus thermophilus have been measured and compared with those of tRNAs from E. coli. Temperature dependences and...

Introduction of antigenic determining 2,4-dinitrophenyl residues into 4-thiouridine, N3-(3-L-amino-3-carboxypropyl) uridine and tRNA-Phe from E. coli.

Seela, F, Hansske, F, Watanabe, K, Cramer, F

The introduction of antigenic determining 2,4-dinitrophenyl residues into the rare ribonucleosides 4-thiouridine (1a), and N3-(3-L-amino-3-carboxypropyl) uridine (2) as well as into tRNA-Phe from E....

CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.

Watanabe, K, Oshima, T, Nishimura, S

5-Methyl-2-thiouridine (S) in tRNA-Met-f from an extreme thermophile is located in the TpsiC region, replacing T, and has a positive CD band centered at 310 nm. Upon heating, the profiles of the...

Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27.

Kumagai, I, Watanabe, K, Oshima, T

The contents of 2'-O-methylguanosine and 1-methyladenosine in unfractionated tRNA obtained from Thermus thermophilus HB27 were found to increase significantly when the bacterium was grown at a higher...

Induction of Allogeneic Unresponsiveness in Adult Dogs: ROLE OF NON-DLA HISTOCOMPATIBILITY VARIABLES IN CONDITIONING THE OUTCOME OF BONE MARROW, KIDNEY, AND SKIN TRANSPLANTATION IN RADIATION CHIMERAS

Rapaport, F. T., Bachvaroff, R. J., Watanabe, K., Hirasawa, H., Mollen, N., Ferrebee, J. W., ...

Exposure to supralethal total body irradiation and transplantation of bone marrow from a DLA- and pedigree-identical donor have regularly produced successful engraftment and the establishment of...

Recent evidence for evolution of the genetic code.

Osawa, S, Jukes, T H, Watanabe, K, Muto, A

The genetic code, formerly thought to be frozen, is now known to be in a state of evolution. This was first shown in 1979 by Barrell et al. (G. Barrell, A. T. Bankier, and J. Drouin, Nature [London]...

Bacteriophage Active Against the Lactic Acid Beverage-Producing Bacterium Lactobacillus casei

Watanabe, K., Takesue, S., Jin-Nai, K., Yoshikawa, T.

A virulent bacteriophage which causes a decrease in acid production during fermentation of a lactic acid beverage named Yakult with Lactobacillus casei was isolated from the abnormal fermentation...

Reticulocyte maturity as an indicator for estimating qualitative abnormality of erythropoiesis.

Watanabe, K, Kawai, Y, Takeuchi, K, Shimizu, N, Iri, H, Ikeda, Y, ...

AIMS--To determine the maturity of reticulocytes in patients with anaemia as a result of various haematological disorders including those with qualitative abnormalities such as ineffective...

Transient abnormal myelopoiesis accompanied by hepatic fibrosis in two infants with Down syndrome.

Yagihashi, N, Watanabe, K, Yagihashi, S

Two necropsy cases of Down syndrome are reported. These showed transient abnormal myelopoiesis accompanying characteristic hepatic sinusoidal fibrosis. Numerous megakaryocytes were found in the liver...

Higher-order structure of bovine mitochondrial tRNA(Phe) lacking the 'conserved' GG and T psi CG sequences as inferred by enzymatic and chemical probing.

Wakita, K, Watanabe, Y, Yokogawa, T, Kumazawa, Y, Nakamura, S, Ueda, T, ...

Bovine mitochondrial (mt) phenylalanine tRNA (tRNA(Phe)), which lacks the 'conserved' GG and T psi YCG sequences, was efficiently purified by the selective hybridization method using a solid phase...

Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis.

Yoshizawa, S, Ueda, T, Ishido, Y, Miura, K, Watanabe, K, Hirao, I

The nuclease resistance of a short, thermostable mini-hairpin, d(GCGAAGC), and other related hairpins was examined. Hairpins possessing a purine-rich (GAA) or (GAAA) loop appeared to be more...

The high content of natural suppressor serine tRNA in dystrophic mouse muscle.

Hitaka, T, Mizutani, T, Watanabe, K, Totsuka, T

In order to gain an insight into the pathogenesis of mouse muscular dystrophy, we investigated the natural suppressor serine tRNA. The natural suppressor seryl-tRNA was distinguished from the other...

The 'polysemous' codon--a codon with multiple amino acid assignment caused by dual specificity of tRNA identity.

Suzuki, T, Ueda, T, Watanabe, K

In some Candida species, the universal CUG leucine codon is translated as serine. However, in most cases, the serine tRNAs responsible for this non-universal decoding (tRNA(Ser)CAG) accept in vitro...

Structural and functional effect of Trp-62-->Gly and Asp-101-->Gly substitutions on substrate-binding modes of mutant hen egg-white lysozymes.

Maenaka, K, Matsushima, M, Kawai, G, Kidera, A, Watanabe, K, Kuroki, R, ...

In order to clarify the structural role of subsite B of hen egg-white lysozyme in hydrolytic activity towards a carbohydrate substrate, we analysed the structures of Trp-62-->Gly and Asp-101-->Gly...

Reducing self-injury and corresponding self-restraint through the strategic use of protective clothing.

Silverman, K, Watanabe, K, Marshall, A M, Baer, D M

We examined the use of protective clothing to reduce a retarded male's face-punching and leg-kicking and two corresponding forms of self-restraint--arm and leg self-restraint. The resident was...

Possible involvement of Escherichia coli 23S ribosomal RNA in peptide bond formation.

Nitta, I, Ueda, T, Watanabe, K

Experimental results are presented suggesting that 23S rRNA is directly involved in the peptide bond formation usually performed on the ribosome. Although several reports have indicated that the...

Concurrent molecular recognition of the amino acid and tRNA by a ribozyme.

Saito, H, Watanabe, K, Suga, H

We have recently reported an in vitro-evolved precursor tRNA (pre-tRNA) that is able to catalyze aminoacylation on its own 3'-hydroxyl group. This catalytic pre-tRNA is susceptible to RNase P RNA,...

The T-loop region of animal mitochondrial tRNA(Ser)(AGY) is a main recognition site for homologous seryl-tRNA synthetase.

Ueda, T, Yotsumoto, Y, Ikeda, K, Watanabe, K

Recognition sites of bovine mitochondrial serine tRNA specific for condons AGY [tRNA(Ser) (AGY)] by the cognate mitochondrial seryl-tRNA synthetase were studied using a range of tRNA(Ser)(AGY)...

Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization.

Kumazawa, Y, Yokogawa, T, Tsurui, H, Miura, K, Watanabe, K

In the course of developing a method to purify a single tRNA species efficiently, we have examined hybridization efficiencies between some tRNAs and short oligodeoxyribonucleotide probes both by the...

Ontogeny of the circadian rhythm of plasma corticosterone in blind infantile rats.

Hiroshige, T, Honma, K, Watanabe, K

1. Post-natal manifestation of plasma corticosterone rhythm was followed by individual pups until the end of 12 weeks of life. Results obtained were analysed by both visual inspection and a least...

Possible zeitgebers for external entrainment of the circadian rhythm of plasma corticosterone in blind infantile rats.

Hiroshige, T, Honma, K, Watanabe, K

1. The phase angle of circadian rhythm of plasma corticosterone in individual blind pups was followed in order to find out an effective zeitgeber(s) during the post-natal development. 2. The retinal...

Prenatal onset and maternal modifications of the circadian rhythm of plasma corticosterone in blind infantile rats.

Hiroshige, T, Honma, K, Watanabe, K

1. In order to clarify the manner in which maternal nursing modifies the circadian rhythm of plasma corticosterone in blind pups, specified schedules of maternal nursing were imposed on blind pups...

Nucleotide Sequence and Gene Organization of the Starfish Asterina Pectinifera Mitochondrial Genome

Asakawa, S., Himeno, H., Miura, K. I., Watanabe, K.

The 16,260-bp mitochondrial DNA (mtDNA) from the starfish Asterina pectinifera has been sequenced. The genes for 13 proteins, two rRNAs and 22 tRNAs are organized in an extremely economical fashion,...

Evolution of Pulmonate Gastropod Mitochondrial Genomes: Comparisons of Gene Organizations of Euhadra, Cepaea and Albinaria and Implications of Unusual Trna Secondary Structures

Yamazaki, N., Ueshima, R., Terrett, J. A., Yokobori, S. I., Kaifu, M., Segawa, R., ...

Complete gene organizations of the mitochondrial genomes of three pulmonate gastropods, Euhadra herklotsi, Cepaea nemoralis and Albinaria coerulea, permit comparisons of their gene organizations....

Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata).

Yokobori, S I, Ueda, T, Feldmaier-Fuchs, G, Pääbo, S, Ueshima, R, Kondow, A, ...

The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to...

Spontaneous loss of heterozygosity in diploid Saccharomyces cerevisiae cells.

Hiraoka, M, Watanabe, K, Umezu, K, Maki, H

To obtain a broad perspective of the events leading to spontaneous loss of heterozygosity (LOH), we have characterized the genetic alterations that functionally inactivated the URA3 marker...

Histogenesis and distribution of red and white muscle fibres of urodelan larvae.

Watanabe, K, Sasaki, F, Takahama, H, Iseki, H

A histochemical and electron microscopical study has been made of the myotomal musculature of an urodelan (Hynobius tokyoensis) tadpole tail and trunk. In older larvae (stage 64) two main types of...

Protective immune responses induced by secretion of a chimeric soluble protein from a recombinant Mycobacterium bovis bacillus Calmette-Guérin vector candidate vaccine for human immunodeficiency virus type 1 in small animals.

Honda, M, Matsuo, K, Nakasone, T, Okamoto, Y, Yoshizaki, H, Kitamura, K, ...

A recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) vector-based vaccine that secretes the V3 principal neutralizing epitope of human immunodeficiency virus (HIV) could induce immune...

One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts.

Melchers, G, Mohri, Y, Watanabe, K, Wakabayashi, S, Harada, K

Mesophyll protoplasts of Lycopersicon esculentum were treated with iodoacetamide to inactivate mitochondria, and protoplasts of Solanum acaule and Solanum tuberosum were irradiated with gamma- or...

Definition of a physiologic aging autoantigen by using synthetic peptides of membrane protein band 3: localization of the active antigenic sites.

Kay, M M, Marchalonis, J J, Hughes, J, Watanabe, K, Schluter, S F

Senescent cell antigen (SCA), an aging antigen, is a protein that appears on old cells and marks them for removal by the immune system in mammals. It is derived from band 3, a ubiquitous membrane...

Antimicrobial Resistance and Serotype Distribution of Streptococcus pneumoniae Strains Causing Childhood Infections in Bangladesh, 1993 to 1997

Saha, Samir K., Rikitomi, N., Ruhulamin, M., Masaki, H., Hanif, M., Islam, Maksuda, ...

Three hundred sixty-two Streptococcus pneumoniae strains were isolated from children under 5 years of age at Dhaka Shishu (Children) Hospital from 1993 to 1997. The strains were isolated from blood...

Preparation of biologically active Ascaris suum mitochondrial tRNAMet with a TV-replacement loop by ligation of chemically synthesized RNA fragments.

Ohtsuki, T, Kawai, G, Watanabe, Y, Kita, K, Nishikawa, K, Watanabe, K

Ascaris suum mitochondrial tRNA Met lacking the entire T stem was prepared by enzymatic ligation of two chemically synthesized RNA fragments. The synthetic tRNA could be charged with methionine by...

An RNA fragment consisting of the P7 and P9.0 stems and the 3'-terminal guanosine of the Tetrahymena group I intron.

Watanabe, S, Kawai, G, Muto, Y, Watanabe, K, Inoue, T, Yokoyama, S

On the basis of the nucleotide sequence of Tetrahymena group I intron, we constructed a 31 residue RNA that has the P7 stem and the 3'-terminal guanosine residue (3'-G) with a putative stem-loop...

RNA editing in the acceptor stem of squid mitochondrial tRNA(Tyr).

Tomita, K, Ueda, T, Watanabe, K

In squid (Loligo bleekeri) mitochondria, the two 3'-terminal nucleotides (G72-G73) of the tRNA(Tyr) gene overlap with the two 5'-terminal nucleotides (G1-G2) of the downstream tRNA(Cys) gene. To...

Expression of bovine mitochondrial tRNASer GCU derivatives in Escherichia coli.

Hayashi, I, Kawai, G, Watanabe, K

By replacing a stretch of five A-U base pairs in the acceptor stem with G-C pairs, mitochondrial tRNA-SerGCU lacking a D arm could be expressed in Escherichia coli cells in considerable amounts. The...

The presence of pseudouridine in the anticodon alters the genetic code: a possible mechanism for assignment of the AAA lysine codon as asparagine in echinoderm mitochondria.

Tomita, K, Ueda, T, Watanabe, K

It has been inferred from DNA sequence analyses that in echinoderm mitochondria not only the usual asparagine codons AAU and AAC, but also the usual lysine codon AAA, are translated as asparagine by...

An extra tRNAGly(U*CU) found in ascidian mitochondria responsible for decoding non-universal codons AGA/AGG as glycine.

Kondow, A, Suzuki, T, Yokobori, S, Ueda, T, Watanabe, K

Amino acid assignments of metazoan mitochondrial codons AGA/AGG are known to vary among animal species; arginine in Cnidaria, serine in invertebrates and stop in vertebrates. We recently found that...

Codon reading patterns in Drosophila melanogaster mitochondria based on their tRNA sequences: a unique wobble rule in animal mitochondria.

Tomita, K, Ueda, T, Ishiwa, S, Crain, P F, McCloskey, J A, Watanabe, K

Mitochondrial (mt) tRNA(Trp), tRNA(Ile), tRNA(Met), tRNA(Ser)GCU, tRNA(Asn)and tRNA(Lys)were purified from Drosophila melanogaster (fruit fly) and their nucleotide sequences were determined....

NP-06: a novel anti-human immunodeficiency virus polypeptide produced by a Streptomyces species.

Chokekijchai, S, Kojima, E, Anderson, S, Nomizu, M, Tanaka, M, Machida, M, ...

From an extract of a Streptomyces culture, we identified and purified a novel compound, NP-06, which is active against human immunodeficiency virus (HIV) in vitro. Analyses indicate that NP-06 is a...

In vitro activities of 10 antimicrobial agents against bacterial vaginosis-associated anaerobic isolates from pregnant Japanese and Thai women.

Puapermpoonsiri, S, Watanabe, K, Kato, N, Ueno, K

The in vitro activities of 10 antimicrobial agents against 159 bacterial vaginosis-associated anaerobic isolates from pregnant Japanese and Thai women were determined. Clindamycin, imipenem,...

Analysis of the critical sites for protein thermostabilization by proline substitution in oligo-1,6-glucosidase from Bacillus coagulans ATCC 7050 and the evolutionary consideration of proline residues.

Watanabe, K, Kitamura, K, Suzuki, Y

To identify the critical sites for protein thermostabilization by proline substitution, the gene for oligo-1,6- glucosidase from a thermophilic Bacillus coagulans strain, ATCC 7050, was cloned as a...

Identification of a functionally important population in phenol-digesting activated sludge with antisera raised against isolated bacterial strains.

Watanabe, K, Hino, S

Antisera were raised against nine strains which had been isolated from phenol-acclimated oil refinery activated sludge. Although several antisera reacted significantly with the activated sludge...

A monoclonal antibody reactive with a common epitope of Moraxella (Branhamella) catarrhalis lipopolysaccharides.

Oishi, K, Tanaka, H, Sonoda, F, Borann, S, Ahmed, K, Utsunomiya, Y, ...

A hybrid cell line producing a monoclonal antibody (MAb) against Moraxella (Branhamella) catarrhalis lipopolysaccharide (LPS) was established. The specificity of the MAb 1B12 to purified rough LPSs...

Macrolide accumulation by Bacteroides fragilis ATCC 25285.

Muto, Y, Bandoh, K, Watanabe, K, Katoh, N, Ueno, K

The accumulation of macrolide antibiotics in Bacteroides fragilis ATCC 25285 was increased in the order erythromycin, josamycin, and rokitamycin, depending on hydrophobicity. The half-times of efflux...

Effects of Clostridium difficile toxin A and toxin B on phospholipase D activation in human promyelocytic leukemic HL60 cells.

Ohguchi, K, Banno, Y, Nakashima, S, Kato, N, Watanabe, K, Lyerly, D M, ...

The possible involvement of Rho family GTP-binding proteins in the regulation of phospholipase D (PLD) activity has recently been demonstrated. In the present study, to further examine the role of...

Emergence of fluoroquinolone resistance in Bacteroides fragilis accompanied by resistance to beta-lactam antibiotics.

Kato, N, Miyauchi, M, Muto, Y, Watanabe, K, Ueno, K

The in vitro susceptibilities of a group of 93 isolates of Bacteroides fragilis collected from 1983 to 1984 to six fluoroquinolones were compared with those of a group of 93 isolates collected from...

Physiological and biochemical analysis of the effects of alkaline phosphatase overproduction in Escherichia coli.

Kadokura, H, Watanabe, K, Tsuneizumi, K, Yoda, K, Yamasaki, M

Overexpression of the Escherichia coli phoA gene, coding for alkaline phosphatase (PhoA), on multicopy plasmids caused a severe defect in the precursor processing (secretion) of PhoA, beta-lactamase,...

Susceptibilities of anaerobic bacteria to N-formimidoyl thienamycin (MK0787) and to other antibiotics.

Kesado, T, Watanabe, K, Asahi, Y, Isono, M, Ueno, K

The susceptibilities of 462 clinical anaerobic bacterial isolates to N-formimidoyl thienamycin and 16 other currently available and investigational antibiotics were determined by the agar dilution...

Comparative antibacterial activities of 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against Bacteroides fragilis.

Kesado, T, Watanabe, K, Asahi, Y, Isono, M, Ueno, K

The in vitro antibacterial activities of the newly developed 7 alpha-methoxy cephalosporins and 7 beta-methoxyiminoacetamido cephalosporins against 67 clinical isolates of Bacteroides fragilis and...

Construction and characterization of a fimA mutant of Porphyromonas gingivalis.

Hamada, N, Watanabe, K, Sasakawa, C, Yoshikawa, M, Yoshimura, F, Umemoto, T

Although fimbriae of Porphyromonas gingivalis have been implicated as playing a major role in adherence to gingival tissue surfaces, no conclusive genetic evidence has yet been obtained. The fimA...

Therapeutic effects of a human antiflagella monoclonal antibody in a neutropenic murine model of Pseudomonas aeruginosa pneumonia.

Oishi, K, Sonoda, F, Iwagaki, A, Ponglertnapagorn, P, Watanabe, K, Nagatake, T, ...

Human immunoglobulin G1 (IgG1) monoclonal antibodies (MAbs) reactive with type-specific Pseudomonas aeruginosa lipopolysaccharide (LPS) and flagella were compared for their protective activities...

In vitro activity of FK037, a new parenteral cephalosporin, against anaerobic bacteria.

Kato, N, Kato, H, Tanaka, Y, Bando, K, Watanabe, K, Ueno, K

The activity of FK037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. A fastidious...

Mechanism for inhibition of influenza virus RNA polymerase activity by matrix protein.

Watanabe, K, Handa, H, Mizumoto, K, Nagata, K

Influenza virus M1 protein has been shown to inhibit the transcription catalyzed by viral ribonucleoprotein complexes isolated from virions. Here, this inhibition mechanism was studied with the...

Use of Loop-Mediated Isothermal Amplification of the IS900 Sequence for Rapid Detection of Cultured Mycobacterium avium subsp. paratuberculosis

Enosawa, M., Kageyama, S., Sawai, K., Watanabe, K., Notomi, T., Onoe, S., ...

We evaluated the usefulness of loop-mediated isothermal amplification (LAMP) in detecting specific gene sequences of Mycobacterium avium subsp. paratuberculosis (MAP). A total of 102 primer sets for...

Identification of dimethyl disulfide-forming bacteria isolated from activated sludge.

Tomita, B, Inoue, H, Chaya, K, Nakamura, A, Hamamura, N, Ueno, K, ...

Twenty-four strains with high dimethyl disulfide (DMDS)-forming ability were isolated from activated sludge and identified to the genus level. These bacteria were classified into four groups (A, B,...

Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.

Watanabe, K, Hayashida, M, Nakashima, Y, Hayashi, S

Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM...

Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius.

Watanabe, K, Iha, H, Ohashi, A, Suzuki, Y

The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a...

Molecular cloning of a gene for indole-3-acetamide hydrolase from Bradyrhizobium japonicum.

Sekine, M, Watanabe, K, Syono, K

A pLAFR1 cosmid genomic library of wild-type Bradyrhizobium japonicum J1063 was constructed. A cosmid clone designated pBjJ4, containing a 26-kilobase (kb) DNA insert, was identified as being able to...

Serotypes of Streptococcus pneumoniae causing invasive childhood infections in Bangladesh, 1992 to 1995.

Saha, S K, Rikitomi, N, Biswas, D, Watanabe, K, Ruhulamin, M, Ahmed, K, ...

One hundred sixty-five invasive Streptococcus pneumoniae strains were isolated from children under five at Dhaka Shishu (Children's) Hospital during the period 1992 to 1995. Ninety-four strains were...

Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains.

Murata, K, Fukuda, Y, Shimosaka, M, Watanabe, K, Saikusa, T, Kimura, A

The gene responsible for the methylglyoxal resistance of Saccharomyces cerevisiae was cloned, and its phenotypic characteristics were investigated. S. cerevisiae cells with the gene could accumulate...

Biochemical properties and purification of metallo-beta-lactamase from Bacteroides fragilis.

Bandoh, K, Muto, Y, Watanabe, K, Katoh, N, Ueno, K

The beta-lactamase from Bacteroides fragilis GAI-30144 hydrolyzed imipenem, oxyiminocephalosporins, cephamycins, and penicillins. Enzyme activity was inhibited by EDTA. Zinc completely reversed...

Level of neutrophil chemotactic factor CINC/gro, a member of the interleukin-8 family, associated with lipopolysaccharide-induced inflammation in rats.

Iida, M, Watanabe, K, Tsurufuji, M, Takaishi, K, Iizuka, Y, Tsurufuji, S

Rat cytokine-induced neutrophil chemoattractant (CINC), which is a counterpart of human gro and belongs to the interleukin-8 family, has been quantified by a new sandwich enzyme-linked immunosorbent...

Antigenic role of stress-induced catalase of Salmonella typhimurium in cell-mediated immunity.

Kagaya, K, Miyakawa, Y, Watanabe, K, Fukazawa, Y

The ability of the H2O2-induced catalase of Salmonella typhimurium to induce cell-mediated immunity against S. typhimurium infection in mice was examined. When exponentially growing cells of S....

Mechanism for candidacidal activity in macrophages activated by recombinant gamma interferon.

Watanabe, K, Kagaya, K, Yamada, T, Fukazawa, Y

Candidacidal activity in macrophages activated by recombinant gamma interferon was examined kinetically in relation to acidification of phagolysosomes. In resident peritoneal macrophages (PMPs) of...

Purification and properties of a 75-kilodalton major protein, an immunodominant surface antigen, from the oral anaerobe Bacteroides gingivalis.

Yoshimura, F, Watanabe, K, Takasawa, T, Kawanami, M, Kato, H

A 75-kilodalton major protein (75K protein) was purified to homogeneity from the cell lysate fraction and the envelope of Bacteroides gingivalis 381. The 75K protein was originally present in the...

Acute joint inflammation in mice after systemic injection of the cell wall, its peptidoglycan, and chemically defined peptidoglycan subunits from various bacteria.

Koga, T, Kakimoto, K, Hirofuji, T, Kotani, S, Ohkuni, H, Watanabe, K, ...

The systemic injection of an aqueous suspension of cell wall or its peptidoglycan (PG)-rich sonicate derived from group A streptococcus and Lactobacillus casei induced acute joint lesions in BALB/c,...

Typing of Clostridium difficile by western immunoblotting with 10 different antisera.

Kato, H, Cavallaro, J J, Kato, N, Bartley, S L, Killgore, G E, Watanabe, K, ...

Western blotting (immunoblotting) with antisera against each of 10 reference serogroups was evaluated as a means of typing Clostridium difficile. A total of 164 clinical isolates of C. difficile were...

Application of typing by pulsed-field gel electrophoresis to the study of Clostridium difficile in a neonatal intensive care unit.

Kato, H, Kato, N, Watanabe, K, Ueno, K, Ushijima, H, Hashira, S, ...

Pulsed-field gel electrophoresis (PFGE) analysis of restriction pattern polymorphism was applied to type Clostridium difficile isolated from neonates hospitalized in a neonatal intensive care unit,...

Isolation of Clostridium pseudotetanicum from a patient with gas gangrene.

Katoh, N, Watanabe, K, Miki, Y, Muro, T, Ueno, K

Clostridium pseudotetanicum was isolated along with Serratia marcescens, Proteus mirabilis, and Staphylococcus epidermidis from a patient suffering from gas gangrene who had been injured in the right...

Structural similarity of bovine lung prostaglandin F synthase to lens epsilon-crystallin of the European common frog.

Watanabe, K, Fujii, Y, Nakayama, K, Ohkubo, H, Kuramitsu, S, Kagamiyama, H, ...

Cloned cDNA sequences specific for prostaglandin F (PGF) synthase have been isolated from a cDNA library of bovine lung mRNA sequences. Nucleotide-sequence analyses of cloned cDNA inserts have...

Low shear stress can initiate von Willebrand factor-dependent platelet aggregation in patients with type IIB and platelet-type von Willebrand disease.

Murata, M, Fukuyama, M, Satoh, K, Fujimura, Y, Yoshioka, A, Takahashi, H, ...

Platelets exposed to shear stress aggregate in the absence of exogenously added agonists, utilizing distinct platelet membrane receptors and ligands depending upon the level of shear stress applied....

The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress.

Ikeda, Y, Handa, M, Kawano, K, Kamata, T, Murata, M, Araki, Y, ...

Exposure of platelets to shear stress leads to aggregation in the absence of exogenous agonists. We have now found that different adhesive proteins and platelet membrane glycoproteins are involved in...

Human salivary gustin is a potent activator of calmodulin-dependent brain phosphodiesterase.

Law, J S, Nelson, N, Watanabe, K, Henkin, R I

Human salivary gustin stimulated activity of brain calmodulin-dependent cyclic nucleotide phosphodiesterase (cAMP PDEase; 3',5'-cyclic-nucleotide phosphodiesterase, EC 3.1.4.17) in a dose-dependent...

Unusual anticodon loop structure found in E.coli lysine tRNA.

Watanabe, K, Hayashi, N, Oyama, A, Nishikawa, K, Ueda, T, Miura, K

Although both tRNA(Lys) and tRNA(Glu) of E. coli possess similar anticodon loop sequences, with the same hypermodified nucleoside 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the first position of...

Characterization of serine and leucine tRNAs in an asporogenic yeast Candida cylindracea and evolutionary implications of genes for tRNA(Ser)CAG responsible for translation of a non-universal genetic code.

Suzuki, T, Ueda, T, Yokogawa, T, Nishikawa, K, Watanabe, K

Five serine and three leucine isoaceptor tRNAs were purified from the asporogenic yeast Candida cylindracea, in which codon CUG is translated as serine instead of leucine, and their primary...

Most compact hairpin-turn structure exerted by a short DNA fragment, d(GCGAAGC) in solution: an extraordinarily stable structure resistant to nucleases and heat.

Hirao, I, Kawai, G, Yoshizawa, S, Nishimura, Y, Ishido, Y, Watanabe, K, ...

The three-dimensional structure of a short DNA fragment, d(GCGAAGC) exhibiting an extraordinarily stable hairpin structure was determined by nuclear magnetic resonance spectroscopy. Two possible...

Non-universal decoding of the leucine codon CUG in several Candida species.

Ohama, T, Suzuki, T, Mori, M, Osawa, S, Ueda, T, Watanabe, K, ...

It has been reported that CUG, a universal leucine codon, is read as serine in an asporogenic yeast, Candida cylindracea. The distribution of this non-universal genetic code in various yeast species...

The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli.

Watanabe, K, Yamano, Y, Murata, K, Kimura, A

The nucleotide sequence of the gsh I gene for gamma-glutamylcysteine synthetase(GSH I) of Escherichia coli B has been determined. The gsh I structural gene contains 1557 bases in length and predicted...

Nucleotide sequence of the gene responsible for D-xylose uptake in Escherichia coli.

Kurose, N, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 363 bp in length, has been determined. It can complement the mutation of Escherichia coli having a decreased activity of D-xylose uptake at low temperature....

The T-loop region of animal mitochondrial tRNA(Ser)(AGY) is a main recognition site for homologous seryl-tRNA synthetase.

Ueda, T, Yotsumoto, Y, Ikeda, K, Watanabe, K

Recognition sites of bovine mitochondrial serine tRNA specific for condons AGY [tRNA(Ser) (AGY)] by the cognate mitochondrial seryl-tRNA synthetase were studied using a range of tRNA(Ser)(AGY)...

Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization.

Kumazawa, Y, Yokogawa, T, Tsurui, H, Miura, K, Watanabe, K

In the course of developing a method to purify a single tRNA species efficiently, we have examined hybridization efficiencies between some tRNAs and short oligodeoxyribonucleotide probes both by the...

Capacity of recombinant gamma interferon to activate macrophages for Salmonella-killing activity.

Kagaya, K, Watanabe, K, Fukazawa, Y

The ability of recombinant gamma interferon (rIFN-gamma) to activate macrophages for Salmonella-killing activity was kinetically examined in relation to phagosome-lysosome fusion and H2O2 generation....

Extraordinary stable structure of short single-stranded DNA fragments containing a specific base sequence: d(GCGAAAGC).

Hirao, I, Nishimura, Y, Naraoka, T, Watanabe, K, Arata, Y, Miura, K

Short single-stranded DNA fragments carrying a GCGAAAGC sequence were found to move unexpectedly faster than other fragments of the same length in electrophoresis on a polyacrylamide gel containing a...

Purification and characterization of two serine isoacceptor tRNAs from bovine mitochondria by using a hybridization assay method.

Yokogawa, T, Kumazawa, Y, Miura, K, Watanabe, K

For large scale preparation of mitochondrial tRNAs, a new hybridization assay method using synthetic oligodeoxyribonucleotide probes (16-17mer) complementary to individual tRNA sequences was...

Complete nucleotide sequence of the E. coli N-acetylneuraminate lyase.

Ohta, Y, Watanabe, K, Kimura, A

The nucleotide sequence of the cloned DNA, 1,243 bp in length coding for N-acetylneuraminate lyase (N-acetylneuraminate pyruvate lyase; NPL) of Escherichia coli has been determined. Nucleotide...

Graphic display of nucleic acid structure by a microcomputer.

Watanabe, K, Yasukawa, K, Iso, K

A microcomputer program for the display of a three dimensional structure of a nucleic acid and a protein has been developed. This program can generate a wire model and a ball and stick model on a...

Stereospecific conversion of prostaglandin D2 to (5Z,13E)-(15S)-9 alpha-11 beta,15-trihydroxyprosta-5,13-dien-1-oic acid (9 alpha,11 beta-prostaglandin F2) and of prostaglandin H2 to prostaglandin F2 alpha by bovine lung prostaglandin F synthase.

Watanabe, K, Iguchi, Y, Iguchi, S, Arai, Y, Hayaishi, O, Roberts, L J

A prostaglandin F (PGF) synthase was recently purified from bovine lung that catalyzed the reduction of both PGH2 and PGD2 but at different active sites on the enzyme. In view of the recent finding...

Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance.

Davanloo, P, Sprinzl, M, Watanabe, K, Albani, M, Kersten, H

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from...

A novel cloverleaf structure found in mammalian mitochondrial tRNA(Ser) (UCN).

Yokogawa, T, Watanabe, Y, Kumazawa, Y, Ueda, T, Hirao, I, Miura, K, ...

Bovine mitochondrial tRNA(Ser) (UCN) has been thought to have two U-U mismatches at the top of the acceptor stem, as inferred from its gene sequence. However, this unusual structure has not been...

Higher-order structure of bovine mitochondrial tRNA(SerUGA): chemical modification and computer modeling.

Watanabe, Y, Kawai, G, Yokogawa, T, Hayashi, N, Kumazawa, Y, Ueda, T, ...

On the basis of enzymatic probing and phylogenetic comparison, we have previously proposed that mammalian mitochondrial tRNA(sSer) (anticodon UGA) possess a slightly altered cloverleaf structure in...

Conversion of aminoacylation specificity from tRNA(Tyr) to tRNA(Ser) in vitro.

Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Shimizu, M

The discrimination mechanism between tRNA(Ser) and tRNA(Tyr) was studied using various in vitro transcripts of E. coli tRNATyr variants. The insertion of only two nucleotides into the variable stem...

Phosphorothioate-containing RNAs show mRNA activity in the prokaryotic translation systems in vitro.

Ueda, T, Tohda, H, Chikazumi, N, Eckstein, F, Watanabe, K

Phosphorothioate-containing RNAs were generated by transcription of coliphage T7 DNA using the Sp diastereomers of ribonucleoside 5'-O-(1-thiotriphosphates) and T7 RNA polymerase. RNAs in which a...

Extraordinarily stable mini-hairpins: electrophoretical and thermal properties of the various sequence variants of d(GCGAAAGC) and their effect on DNA sequencing.

Hirao, I, Nishimura, Y, Tagawa, Y, Watanabe, K, Miura, K

A small DNA fragment having a characteristic sequence d(GCGAAAGC) has been shown to form an extraordinarily stable mini-hairpin structure and to have an unusually rapid mobility in polyacrylamide gel...

Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation.

Himeno, H, Hasegawa, T, Ueda, T, Watanabe, K, Miura, K, Shimizu, M

All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate the role of this unique structure in aminoacylation, we constructed in...

Proton magnetic resonance spectra of tRNA-Met-f from Thermus thermophilus.

Kyogoku, Y, Inubushi, T, Morishima, I, Watanabe, K, Oshima, T, Nishimura, S

220 MHz proton magnetic resonance spectra of tRNAs in bulk and tRNA-Met-f from Thermus thermophilus have been measured and compared with those of tRNAs from E. coli. Temperature dependences and...

Introduction of antigenic determining 2,4-dinitrophenyl residues into 4-thiouridine, N3-(3-L-amino-3-carboxypropyl) uridine and tRNA-Phe from E. coli.

Seela, F, Hansske, F, Watanabe, K, Cramer, F

The introduction of antigenic determining 2,4-dinitrophenyl residues into the rare ribonucleosides 4-thiouridine (1a), and N3-(3-L-amino-3-carboxypropyl) uridine (2) as well as into tRNA-Phe from E....

CD spectra of 5-methyl-2-thiouridine in tRNA-Met-f from an extreme thermophile.

Watanabe, K, Oshima, T, Nishimura, S

5-Methyl-2-thiouridine (S) in tRNA-Met-f from an extreme thermophile is located in the TpsiC region, replacing T, and has a positive CD band centered at 310 nm. Upon heating, the profiles of the...

Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27.

Kumagai, I, Watanabe, K, Oshima, T

The contents of 2'-O-methylguanosine and 1-methyladenosine in unfractionated tRNA obtained from Thermus thermophilus HB27 were found to increase significantly when the bacterium was grown at a higher...

Induction of Allogeneic Unresponsiveness in Adult Dogs: ROLE OF NON-DLA HISTOCOMPATIBILITY VARIABLES IN CONDITIONING THE OUTCOME OF BONE MARROW, KIDNEY, AND SKIN TRANSPLANTATION IN RADIATION CHIMERAS

Rapaport, F. T., Bachvaroff, R. J., Watanabe, K., Hirasawa, H., Mollen, N., Ferrebee, J. W., ...

Exposure to supralethal total body irradiation and transplantation of bone marrow from a DLA- and pedigree-identical donor have regularly produced successful engraftment and the establishment of...

Recent evidence for evolution of the genetic code.

Osawa, S, Jukes, T H, Watanabe, K, Muto, A

The genetic code, formerly thought to be frozen, is now known to be in a state of evolution. This was first shown in 1979 by Barrell et al. (G. Barrell, A. T. Bankier, and J. Drouin, Nature [London]...

Bacteriophage Active Against the Lactic Acid Beverage-Producing Bacterium Lactobacillus casei

Watanabe, K., Takesue, S., Jin-Nai, K., Yoshikawa, T.

A virulent bacteriophage which causes a decrease in acid production during fermentation of a lactic acid beverage named Yakult with Lactobacillus casei was isolated from the abnormal fermentation...

Reticulocyte maturity as an indicator for estimating qualitative abnormality of erythropoiesis.

Watanabe, K, Kawai, Y, Takeuchi, K, Shimizu, N, Iri, H, Ikeda, Y, ...

AIMS--To determine the maturity of reticulocytes in patients with anaemia as a result of various haematological disorders including those with qualitative abnormalities such as ineffective...

Transient abnormal myelopoiesis accompanied by hepatic fibrosis in two infants with Down syndrome.

Yagihashi, N, Watanabe, K, Yagihashi, S

Two necropsy cases of Down syndrome are reported. These showed transient abnormal myelopoiesis accompanying characteristic hepatic sinusoidal fibrosis. Numerous megakaryocytes were found in the liver...

Higher-order structure of bovine mitochondrial tRNA(Phe) lacking the 'conserved' GG and T psi CG sequences as inferred by enzymatic and chemical probing.

Wakita, K, Watanabe, Y, Yokogawa, T, Kumazawa, Y, Nakamura, S, Ueda, T, ...

Bovine mitochondrial (mt) phenylalanine tRNA (tRNA(Phe)), which lacks the 'conserved' GG and T psi YCG sequences, was efficiently purified by the selective hybridization method using a solid phase...

Nuclease resistance of an extraordinarily thermostable mini-hairpin DNA fragment, d(GCGAAGC) and its application to in vitro protein synthesis.

Yoshizawa, S, Ueda, T, Ishido, Y, Miura, K, Watanabe, K, Hirao, I

The nuclease resistance of a short, thermostable mini-hairpin, d(GCGAAGC), and other related hairpins was examined. Hairpins possessing a purine-rich (GAA) or (GAAA) loop appeared to be more...

The high content of natural suppressor serine tRNA in dystrophic mouse muscle.

Hitaka, T, Mizutani, T, Watanabe, K, Totsuka, T

In order to gain an insight into the pathogenesis of mouse muscular dystrophy, we investigated the natural suppressor serine tRNA. The natural suppressor seryl-tRNA was distinguished from the other...

The 'polysemous' codon--a codon with multiple amino acid assignment caused by dual specificity of tRNA identity.

Suzuki, T, Ueda, T, Watanabe, K

In some Candida species, the universal CUG leucine codon is translated as serine. However, in most cases, the serine tRNAs responsible for this non-universal decoding (tRNA(Ser)CAG) accept in vitro...

Nucleotide Sequence and Gene Organization of the Starfish Asterina Pectinifera Mitochondrial Genome

Asakawa, S., Himeno, H., Miura, K. I., Watanabe, K.

The 16,260-bp mitochondrial DNA (mtDNA) from the starfish Asterina pectinifera has been sequenced. The genes for 13 proteins, two rRNAs and 22 tRNAs are organized in an extremely economical fashion,...

Evolution of Pulmonate Gastropod Mitochondrial Genomes: Comparisons of Gene Organizations of Euhadra, Cepaea and Albinaria and Implications of Unusual Trna Secondary Structures

Yamazaki, N., Ueshima, R., Terrett, J. A., Yokobori, S. I., Kaifu, M., Segawa, R., ...

Complete gene organizations of the mitochondrial genomes of three pulmonate gastropods, Euhadra herklotsi, Cepaea nemoralis and Albinaria coerulea, permit comparisons of their gene organizations....

Structural and functional effect of Trp-62-->Gly and Asp-101-->Gly substitutions on substrate-binding modes of mutant hen egg-white lysozymes.

Maenaka, K, Matsushima, M, Kawai, G, Kidera, A, Watanabe, K, Kuroki, R, ...

In order to clarify the structural role of subsite B of hen egg-white lysozyme in hydrolytic activity towards a carbohydrate substrate, we analysed the structures of Trp-62-->Gly and Asp-101-->Gly...

Histogenesis and distribution of red and white muscle fibres of urodelan larvae.

Watanabe, K, Sasaki, F, Takahama, H, Iseki, H

A histochemical and electron microscopical study has been made of the myotomal musculature of an urodelan (Hynobius tokyoensis) tadpole tail and trunk. In older larvae (stage 64) two main types of...

Ontogeny of the circadian rhythm of plasma corticosterone in blind infantile rats.

Hiroshige, T, Honma, K, Watanabe, K

1. Post-natal manifestation of plasma corticosterone rhythm was followed by individual pups until the end of 12 weeks of life. Results obtained were analysed by both visual inspection and a least...

Possible zeitgebers for external entrainment of the circadian rhythm of plasma corticosterone in blind infantile rats.

Hiroshige, T, Honma, K, Watanabe, K

1. The phase angle of circadian rhythm of plasma corticosterone in individual blind pups was followed in order to find out an effective zeitgeber(s) during the post-natal development. 2. The retinal...

Prenatal onset and maternal modifications of the circadian rhythm of plasma corticosterone in blind infantile rats.

Hiroshige, T, Honma, K, Watanabe, K

1. In order to clarify the manner in which maternal nursing modifies the circadian rhythm of plasma corticosterone in blind pups, specified schedules of maternal nursing were imposed on blind pups...

Reducing self-injury and corresponding self-restraint through the strategic use of protective clothing.

Silverman, K, Watanabe, K, Marshall, A M, Baer, D M

We examined the use of protective clothing to reduce a retarded male's face-punching and leg-kicking and two corresponding forms of self-restraint--arm and leg self-restraint. The resident was...

Possible involvement of Escherichia coli 23S ribosomal RNA in peptide bond formation.

Nitta, I, Ueda, T, Watanabe, K

Experimental results are presented suggesting that 23S rRNA is directly involved in the peptide bond formation usually performed on the ribosome. Although several reports have indicated that the...

Concurrent molecular recognition of the amino acid and tRNA by a ribozyme.

Saito, H, Watanabe, K, Suga, H

We have recently reported an in vitro-evolved precursor tRNA (pre-tRNA) that is able to catalyze aminoacylation on its own 3'-hydroxyl group. This catalytic pre-tRNA is susceptible to RNase P RNA,...

Complete DNA sequence of the mitochondrial genome of the ascidian Halocynthia roretzi (Chordata, Urochordata).

Yokobori, S I, Ueda, T, Feldmaier-Fuchs, G, Pääbo, S, Ueshima, R, Kondow, A, ...

The complete nucleotide sequence of the 14,771-bp-long mitochondrial (mt) DNA of a urochordate (Chordata)-the ascidian Halocynthia roretzi-was determined. All the Halocynthia mt-genes were found to...

Spontaneous loss of heterozygosity in diploid Saccharomyces cerevisiae cells.

Hiraoka, M, Watanabe, K, Umezu, K, Maki, H

To obtain a broad perspective of the events leading to spontaneous loss of heterozygosity (LOH), we have characterized the genetic alterations that functionally inactivated the URA3 marker...

Studies on the pathogenicity of anaerobes, especially Prevotella bivia, in a rat pyometra model.

Mikamo, H, Kawazoe, K, Izumi, K, Watanabe, K, Ueno, K, Tamaya, T

OBJECTIVE: Prevotella bivia is one of the anaerobic bacteria that resides in the flora of the female genital tract. We studied the pathogenicity of P. bivia in a rat pyometra model. METHODS: The...

Analysis of autoantibodies to glycolipids on thymocytes in New Zealand black mice.

Ishii, N, Watanabe, K

Carbohydrate specificities of thymocyte cytotoxic autoantibodies in New Zealand Black (NZB) mice were examined using liposome immune lysis assay (LILA) system. NZB mice progressively produced...

In vivo binding of circulating immune complexes by C3b receptors (CR1) of transfused erythrocytes.

Inada, Y, Kamiyama, M, Kanemitsu, T, Ikegami, H, Watanabe, K, Clark, W S, ...

The effects of packed erythrocyte transfusion with high CR1 activity on circulating immune complex concentrations were studied in 14 transfusion experiments involving 12 patients with immune complex...

Complement activating properties of monoreactive and polyreactive IgM rheumatoid factors.

Sato, Y, Sato, R, Watanabe, H, Kogure, A, Watanabe, K, Nishimaki, T, ...

OBJECTIVES--To estimate the complement activating properties of monoclonal, monoreactive, and polyreactive IgM rheumatoid factors derived from Epstein-Barr virus transformed B cells isolated from...

Aromatase in human endometrial carcinoma and hyperplasia. Immunohistochemical, in situ hybridization, and biochemical studies.

Watanabe, K., Sasano, H., Harada, N., Ozaki, M., Niikura, H., Sato, S., ...

The expression of P450 aromatase and other steroidogenic enzymes were evaluated in 42 endometrioid endometrial carcinomas, 23 endometrial hyperplasias, and 7 normal endometrial specimens. These...

Reduced myelinated fiber size correlates with loss of axonal neurofilaments in peripheral nerve of chronically streptozotocin diabetic rats.

Yagihashi, S., Kamijo, M., Watanabe, K.

Peripheral sensory nerve abnormalities were investigated in long-term streptozotocin diabetic rats using quantitative analysis. To determine whether the characteristic structural changes occur with a...

Liver dysfunction among workers handling 5-nitro-o-toluidine

Shimizu, H, Kumada, T, Nakano, S, Kiriyama, S, Sone, Y, Honda, T, ...

Background: 5-Nitro-o-toluidine is an aromatic nitro amino compound. While other aromatic compounds are known to damage the human liver and are registered as toxic substances, toxicity information...

Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.

Osamura, R. Y., Watanabe, K., Nakai, Y., Imura, H.

The comparative immunohistochemical localization of adrenocorticotropic hormone (ACTH) and beta-endorphin-like immunoreactivity (referred to as beta-endorphin) was studied in 16 human anterior...

The persistence and phenotypic stability of transplanted rat liver neoplastic nodules.

Williams, G. M., Ohmori, T., Watanabe, K.

Fragments of normal rat liver and nodular hepatocellular lesions produced by feeding rats 0.02% N-2-fluorenylacetamide for 20 weeks were transplanted into the inguinal mammary fat pad of syngeneic...

IL-8 as an important chemoattractant for neutrophils in ulcerative colitis and Crohn's disease.

Mitsuyama, K, Toyonaga, A, Sasaki, E, Watanabe, K, Tateishi, H, Nishiyama, T, ...

IL-8 is generating increasing interest as a powerful neutrophil chemoattractant and activator. To elucidate the mechanisms of neutrophil infiltration in inflammatory bowel disease, we examined 33...

IgA plasma cells in biliary mucosa: a likely source of locally synthesized IgA in human hepatic bile.

Nagura, H, Tsutsumi, Y, Hasegawa, H, Watanabe, K, Nakane, P K, Brown, W R

IgA synthesized in hepatobiliary tissues accounts for about one-half of the IgA present in human hepatic bile, but the location of the IgA synthesizing cells has been in doubt because few plasma...

CTLA-4 blockade inhibits induction of Helicobacter pylori-associated gastritis in mice

WATANABE, K, MURAKAMI, K, SATO, R, OKIMOTO, T, MAEDA, K, NASU, M, ...

The balance between Th1 and Th2 response determines the outcome of Helicobacter pylori infection. Interferon (IFN)-γ plays an inductive role in gastric inflammation, whereas interleukin (IL)-4...

Enhanced expression and production of monocyte chemoattractant protein-1 in myocarditis

Fuse, K, Kodama, M, Hanawa, H, Okura, Y, Ito, M, Shiono, T, ...

Monocyte chemoattractant protein-1 (MCP-1) is a member of the C-C chemokine family that has been shown to play a major role in the migration of monocytes and T cells to an inflammatory focus. To...

γδ T cells assist αβ T cells in the adoptive transfer of contact hypersensitivity to para-phenylenediamine

Yokozeki, H, Watanabe, K, Igawa, K, Miyazaki, Y, Katayama, I, Nishioka, K

Para-phenylenediamine (PPD) is known to be a common sensitizer of allergic contact dermatitis and contact urticaria. To clarify the mechanism of contact hypersensitivity (CHS) to PPD, we established...

Downregulation of prostaglandin E receptor subtype EP3 during colon cancer development

Shoji, Y, Takahashi, M, Kitamura, T, Watanabe, K, Kawamori, T, Maruyama, T, ...

Background and aims: Involvement of prostaglandin E2 (PGE2) receptors EP1, EP2, and EP4 in the formation of aberrant crypt foci (ACF) and/or intestinal polyps has been suggested. In contrast, EP3...

Pharmacological activity of the C-terminal and N-terminal domains of secretory leukoprotease inhibitor in vitro.

Masuda, K., Kamimura, T., Watanabe, K., Suga, T., Kanesaki, M., Takeuchi, A., ...

1. In order to characterize the physiological functions of the domain structure of secretory leukoprotease inhibitor (SLPI), the biological capacities of half-length SLPIs, (Ser1-Pro54)SLPI and...

Infiltration of neutrophils by intrapleural injection of tumour necrosis factor, interleukin-1, and interleukin-8 in rats, and its modification by actinomycin D.

Utsunomiya, I., Ito, M., Watanabe, K., Tsurufuji, S., Matsushima, K., Oh, S.

1. To assess in vivo chemotactic activity of tumour necrosis factor (TNF), interleukin-1 (IL-1), IL-8, and cytokine-induced neutrophil chemoattractant (CINC), we injected these cytokines into the...

Differential effects of Na+, K(+)-ATPase inhibition by ouabain on acid secretory responses to histamine and bethanechol in the mouse isolated stomach.

Horie, S., Yano, S., Watanabe, K.

1. The effect of Na+,K(+)-ATPase inhibition by ouabain on gastric acid secretion was studied in the mouse isolated whole stomach preparation. 2. Ouabain caused a transient enhancement of...

Effects of glutamic acid analogues on identifiable giant neurones, sensitive to beta-hydroxy-L-glutamic acid, of an African giant snail (Achatina fulica Férussac).

Nakajima, T., Nomoto, K., Ohfune, Y., Shiratori, Y., Takemoto, T., Takeuchi, H., ...

The effects of the seven glutamic acid analogues, alpha-kainic acid, alpha-allo-kainic acid, domoic acid, erythro-L-tricholomic acid, DL-ibotenic acid, L-quisqualic acid and...

Influence of dopamine receptor agonists on gastric acid secretion induced by intraventricular administration of thyrotropin-releasing hormone in the perfused stomach of anaesthetized rats.

Maeda-Hagiwara, M., Watanabe, K.

1 The influence of dopamine receptor agonists on gastric acid secretion stimulated by thyrotropin-releasing hormone (TRH) was studied in the perfused stomach of anaesthetized rats. 2 Intraventricular...

Enhancement by intracerebroventricular thyrotropin-releasing hormone of indomethacin-induced gastric lesions in the rat.

Maeda-Hagiwara, M., Watanabe, H., Watanabe, K.

Effects of the intracerebroventricular thyrotropin-releasing hormone (TRH) on gastric mucosa were studied in rats. TRH (3 and 10 micrograms rat-1 i.c.v.) produced slight gastric lesions and also...

Natural killer (NK) lymphocytosis induced by Epstein–Barr virus (EBV) reactivation in monoclonal gammopathy of undertermined significance (MGUS)

ISHIYAMA, T, KOIKE, M, WATANABE, K, WATANABE, H, FUKUCHI, K, TSURUOKA, N

We investigated the relationship between NK lymphocytosis and EBV infection in MGUS. We found that two out of 10 patients showed an increase of NK cells compared with normal controls. In addition,...

Heat shock-enhanced T cell apoptosis with heat shock protein 70 on T cell surface in multicentric Castleman's disease

ISHIYAMA, T, KOIKE, M, AKIMOTO, Y, FUKUCHI, K, WATANABE, K, YOSHIDA, M, ...

We report here that T cells from patients with multicentric Castleman's disease (MCD) are sensitive to hyperthermia. T cells from two of three patients with MCD revealed DNA ladder formation and...

The conduction velocity of slower and the fastest fibres in infancy and childhood.

Hakamada, S, Kumagai, T, Watanabe, K, Koike, Y, Hara, K, Miyazaki, S

Motor nerve conduction velocity of the ulnar nerve was measured in 54 infants of various ages. Conduction velocity of slower fibres was measured using Hopf's technique, and maximal conduction...

De novo tandem duplication 9p (p12----p24) with normal GALT activity in red cells.

Motegi, T, Watanabe, K, Nakamura, N, Hasegawa, T, Yanagawa, Y

A 3 month old boy with a tandem duplication 9p (p12----p24) is reported. Both clinical and dermatoglyphic features were consistent with those of the trisomy 9p syndrome. However, the red cell...

Deletion (13)(q13q14.3) with retinoblastoma: confirmation and extension of a recognisable pattern of clinical features in retinoblastoma patients with 13q deletion.

Motegi, T, Ikeda, K, Watanabe, K, Yanagawa, Y, Minoda, K

A girl with retinoblastoma and a del(13)(q13q14.3) is presented. This case helps to confirm and extend our previous observations regarding a recognisable facial pattern in retinoblastoma patients...

Novel amyloid precursor protein gene missense mutation (D678N) in probable familial Alzheimer's disease

Wakutani, Y, Watanabe, K, Adachi, Y, Wada-Isoe, K, Urakami, K, Ninomiya, H, ...

Subject: The proband was a women of 72. Symptoms of dementia that fulfilled the criteria for probable Alzheimer's disease appeared at about 60 years of age, and slowly worsened over more than 10...

Bone marrow suppression induced by high dose valproic acid.

Kishi, T, Fujita, N, Kawaguchi, H, Ishimae, M, Watanabe, K, Tanaka, T

A girl with intractable epilepsy who received high dose valproic acid showed bone marrow suppression, which improved after the end of valproic acid treatment and the administration of supportive...

Benign partial epilepsy in infancy.

Okumura, A, Hayakawa, F, Kuno, K, Watanabe, K

The aim was to examine the occurrence of benign partial epilepsy in infancy (BPEI). BPEI was defined as epilepsies with complex partial seizures (CPS) or secondary generalised seizures (SGS), or...

Background electroencephalographic (EEG) activities of very preterm infants born at less than 27 weeks gestation: a study on the degree of continuity

Hayakawa, M, Okumura, A, Hayakawa, F, Watanabe, K, Ohshiro, M, Kato, Y, ...

AIMS—To clarify the features of the background electroencephalographic (EEG) activities in clinically well preterm infants born at less than 27 weeks gestation and to outline their chronological...

HISTOCOMPATIBILITY STUDIES IN A CLOSELY BRED COLONY OF DOGS : IV. TOLERANCE TO BONE MARROW, KIDNEY, AND SKIN ALLOGRAFTS IN DL-A-IDENTICAL RADIATION CHIMERAS

Rapaport, F. T., Watanabe, K., Cannon, F. D., Mollen, N., Blumenstock, D., Ferrebee, J. W.

17 Cooperstown beagles of known DL-A genotypes were exposed to supralethal total body irradiation and received a bone marrow allograft from a DL-A-identical donor; 11 littermate and 6 nonlittermate...

Tax Policy and Consumer Spending: Evidence from Japanese Fiscal Experiments

K. Watanabe, T. Watanabe, T. Watanabe

This paper studies the extent to which the impact on consumer spending differs between temporary and permanent, as well as anticipated and unanticipated tax changes. To discriminate between them, we...

Augmented expression of secondary lymphoid tissue chemokine and EBI1 ligand chemokine in Crohn’s disease

Kawashima, D, Oshitani, N, Jinno, Y, Watanabe, K, Nakamura, S, Higuchi, K, ...

Background: A dominant T helper type 1 (Th1) immune response is thought to be involved in Crohn’s disease (CD). SLC/CCL21 and ELC/CCL19, chemokines that regulate T cell homing and promote...

Distant metastasis facilitated by BCG: spread of tumour cells injected in the BCG-primed site.

Ishibashi, T., Yamada, H., Harada, S., Harada, Y., Miyazaki, N., Takamoto, M., ...

Tumour metastasis in BCG-pretreated mice was studied using a methylcholanthrene-induced fibrosarcoma in C3H/He mice. When tumour cells were injected into the BCG-primed site, distant metastasis...

Randomised phase III trial of carboplatin plus etoposide vs split doses of cisplatin plus etoposide in elderly or poor-risk patients with extensive disease small-cell lung cancer: JCOG 9702

Okamoto, H, Watanabe, K, Kunikane, H, Yokoyama, A, Kudoh, S, Asakawa, T, ...

We compared the efficacy and the safety of a carboplatin plus etoposide regimen (CE) vs split doses of cisplatin plus etoposide (SPE) in elderly or poor-risk patients with extensive disease...

Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene

Kato, H, Zhou, Y, Asanoma, K, Kondo, H, Yoshikawa, Y, Watanabe, K, ...

To obtain functional evidence for DCC as a tumour suppressor associated with endometrial cancer, the human DCC cDNA encoding a complete open reading frame (ORF) was transfected into highly...