Efficient site-directed mutagenesis by simultaneous use of two primers (1983)
Norris, Kjeld, Norris, Fanny, Christiansen, Lars, Fiil, Niels
A rapid and efficient procedure for site specific mutagenesis is described. A double primed synthesis with a 17-mer mismatch primer and a “universal” 15-mer M13 sequencing primer was used to...