Belfort-Neto,Rubens, Nussenblatt,Veronique, Rizzo,Luiz, Muccioli,Cristina, Silveira,Claudio, Nussenblatt,Robert, ...
Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in...
Artemisinin-Resistant Mutants of Toxoplasma gondii Have Altered Calcium Homeostasis � (2007)
Kisaburo Nagamune, L. David Sibley
Artemisinin is a plant sesquiterpene lactone that has become an important drug for combating malaria, especially in regions where resistance to other drugs is widespread. While the mechanism of...
Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii (2006)
Khan, Asis, Böhme, Ulrike, Kelly, Krystyna A., Adlem, Ellen, Brooks, Karen, Simmonds, Mark, ...
Toxoplasma gondii is a globally distributed protozoan parasite that can infect virtually all warm-blooded animals and humans. Despite the existence of a sexual phase in the life cycle, T. gondii has...
Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii (2006)
Khan, Asis, Böhme, Ulrike, Kelly, Krystyna A., Adlem, Ellen, Brooks, Karen, Simmonds, Mark, ...
Toxoplasma gondii is a globally distributed protozoan parasite that can infect virtually all warm-blooded animals and humans. Despite the existence of a sexual phase in the life cycle, T. gondii has...
Nagamune, Kisaburo, Sibley, L. David
The phylum Apicomplexa comprises a large group of early branching eukaryotes that includes a number of human and animal parasites. Calcium controls a number of vital processes in apicomplexans...
Gordon, Jennifer L, Sibley, L David
Abstract Background The phylum Apicomplexa is an early-branching eukaryotic lineage that contains a number of important human and animal pathogens. Their complex life cycles and unique cytoskeletal...
Khan, Asis, Taylor, Sonya, Su, Chunlei, Mackey, Aaron J., Boyle, Jon, Cole, Robert, ...
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and...
ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites (2004)
Li Li, Jonathan Crabtree, Steve Fischer, Deborah Pinney, Christian J. Stoeckert, L. David Sibley, ...
ApiEST-DB
ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites (2004)
Li, Li, Crabtree, Jonathan, Fischer, Steve, Pinney, Deborah, Stoeckert, Christian J., Sibley, L. David, ...
ApiEST‐DB (http://www.cbil.upenn.edu/paradbs‐servlet/) provides integrated access to publicly available EST data from protozoan parasites in the phylum Apicomplexa. The database currently...
Dinitroanilines Bind �-Tubulin to Disrupt (2003)
Naomi S. Morrissette, Arpita Mitra, David Sept, L. David Sibley
Protozoan parasites are remarkably sensitive to dinitroanilines such as oryzalin, which disrupt plant but not animal microtubules. To explore the basis of dinitroaniline action, we isolated 49...
Li, Li, Brunk, Brian P., Kissinger, Jessica C., Pape, Deana, Tang, Keliang, Cole, Robert H., ...
Large-scale EST sequencing projects for several important parasites within the phylum Apicomplexa were undertaken for the purpose of gene discovery. Included were several parasites of medical...
Ajioka, James W., Boothroyd, John C., Brunk, Brian P., Hehl, Adrian, Hillier, Ledeana, Manger, Ian D., ...
Thesis (M.A.)--Northern Michigan University, 1982.
Lecordier, Laurence, Mercier, Corinne, Sibley, L. David, Cesbron-Delauw, Marie-France
The intracellular parasite Toxoplasma gondii resides within a specialized compartment, the parasitophorous vacuole (PV), that resists fusion with host cell endocytic and lysosomal compartments. The...
Håkansson, Sebastian, Morisaki, Hiroshi, Heuser, John, Sibley, L. David
Toxoplasma gondii is a member of the phylum Apicomplexa, a diverse group of intracellular parasites that share a unique form of gliding motility. Gliding is substrate dependent and occurs without...
Sahoo, Nivedita, Beatty, Wandy, Heuser, John, Sept, David, Sibley, L. David
Toxoplasma is a protozoan parasite in the phylum Apicomplexa, which contains a number of medically important parasites that rely on a highly unusual form of motility termed gliding to actively...
Toxoplasma gondii Uses Sulfated Proteoglycans for Substrate and Host Cell Attachment
Carruthers, Vern B., Håkansson, Sebastian, Giddings, Olivia K., Sibley, L. David
Toxoplasma gondii is an obligate intracellular parasite that actively invades a wide variety of vertebrate cells, although the basis of this pervasive cell recognition is not understood. We...
Manger, Ian D., Hehl, Adrian, Parmley, Steve, Sibley, L. David, Marra, Marco, Hillier, Ladeana, ...
Toxoplasma gondii is a protozoan parasite responsible for widespread infections in humans and animals. Two major asexual forms are produced during the life cycle of this parasite: the rapidly...
Targeted Disruption of the GRA2 Locus in Toxoplasma gondii Decreases Acute Virulence in Mice
Mercier, Corinne, Howe, Daniel K., Mordue, Dana, Lingnau, Maren, Sibley, L. David
Following invasion into the host cell, the protozoan Toxoplasma gondii secretes a variety of proteins that modify the parasitophorous vacuole. Within the vacuole, the 28-kDa dense granule protein...
Howe, Daniel K., Crawford, Amy C., Lindsay, David, Sibley, L. David
Neospora caninum is an apicomplexan parasite that is closely related to Toxoplasma gondii and has been found to be associated with neurological disorders in dogs and congenital infections and...
Biogenesis of Nanotubular Network in Toxoplasma Parasitophorous Vacuole Induced by Parasite Proteins
Mercier, Corinne, Dubremetz, Jean-François, Rauscher, Béatrice, Lecordier, Laurence, Sibley, L. David, Cesbron-Delauw, Marie-France
The intracellular parasite Toxoplasma gondii develops within a nonfusogenic vacuole containing a network of elongated nanotubules that form connections with the vacuolar membrane. Parasite secretory...
Howe, Daniel K., Tang, Keliang, Conrad, Patricia A., Sverlow, Karen, Dubey, J. P., Sibley, L. David
Neosporosis is an economically important disease of dairy cattle caused by the protozoan Neospora caninum. Diagnostic tests for neosporosis are complicated by the potential for cross-reaction of...
Cytoskeleton of Apicomplexan Parasites
Morrissette, Naomi S., Sibley, L. David
The Apicomplexa are a phylum of diverse obligate intracellular parasites including Plasmodium spp., the cause of malaria; Toxoplasma gondii and Cryptosporidium parvum, opportunistic pathogens of...
Identification of quantitative trait loci controlling acute virulence in Toxoplasma gondii
Su, Chunlei, Howe, Daniel K., Dubey, J. P., Ajioka, James W., Sibley, L. David
Strains of Toxoplasma gondii can be grouped into three predominant clonal lineages with members of the type I group being uniformly lethal in mice. To elucidate the basis of this extreme virulence, a...
Asai, Takashi, Takeuchi, Tsutomu, Diffenderfer, Jeff, Sibley, L. David
Approximately 150,000 small-molecule compounds were tested by a robotic screening assay for their ability to inhibit nucleoside triphosphate hydrolase (NTPase), a novel enzyme of the tachyzoite form...
Toxoplasma evacuoles: a two-step process of secretion and fusion forms the parasitophorous vacuole
Håkansson, Sebastian, Charron, Audra J., Sibley, L.David
Rapid discharge of secretory organelles called rhoptries is tightly coupled with host cell entry by the protozoan parasite Toxoplasma gondii. Rhoptry contents were deposited in clusters of vesicles...
Huynh, My-Hang, Rabenau, Karen E., Harper, Jill M., Beatty, Wandy L., Sibley, L.David, Carruthers, Vern B.
Vertebrate cells are highly susceptible to infection by obligate intracellular parasites such as Toxoplasma gondii, yet the mechanism by which these microbes breach the confines of their target cell...
ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites
Li, Li, Crabtree, Jonathan, Fischer, Steve, Pinney, Deborah, Stoeckert, Christian J., Sibley, L. David, ...
ApiEST-DB (http://www.cbil.upenn.edu/paradbs-servlet/) provides integrated access to publicly available EST data from protozoan parasites in the phylum Apicomplexa. The database currently...
Dinitroanilines Bind α-Tubulin to Disrupt Microtubules
Morrissette, Naomi S., Mitra, Arpita, Sept, David, Sibley, L. David
Protozoan parasites are remarkably sensitive to dinitroanilines such as oryzalin, which disrupt plant but not animal microtubules. To explore the basis of dinitroaniline action, we isolated 49...
Li, Li, Brunk, Brian P., Kissinger, Jessica C., Pape, Deana, Tang, Keliang, Cole, Robert H., ...
Large-scale EST sequencing projects for several important parasites within the phylum Apicomplexa were undertaken for the purpose of gene discovery. Included were several parasites of medical...
Brossier, Fabien, Jewett, Travis J., Sibley, L. David, Urban, Sinisa
Apicomplexan parasites cause serious human and animal diseases, the treatment of which requires identification of new therapeutic targets. Host-cell invasion culminates in the essential cleavage of...
Khan, Asis, Taylor, Sonya, Su, Chunlei, Mackey, Aaron J., Boyle, Jon, Cole, Robert, ...
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and...
Gliding Motility Leads to Active Cellular Invasion by Cryptosporidium parvum Sporozoites
Wetzel, Dawn M., Schmidt, Joann, Kuhlenschmidt, Mark S., Dubey, J. P., Sibley, L. David
We examined gliding motility and cell invasion by an early-branching apicomplexan, Cryptosporidium parvum, which causes diarrheal disease in humans and animals. Real-time video microscopy...
Khan, A., Su, C., German, M., Storch, G. A., Clifford, D. B., Sibley, L. David
Toxoplasma gondii is an important food- and waterborne opportunistic pathogen that causes severe disease in immunocompromised patients. T. gondii has an unusual clonal population structure consisting...
Nowakowska, Dorota, Colón, Iris, Remington, Jack S., Grigg, Michael, Golab, Elzbieta, Wilczynski, J., ...
Toxoplasma gondii has a clonal population genetic structure with three (I, II, and III) lineages that predominate in North America and Europe. Type II strains cause most cases of symptomatic human...
Lecordier, Laurence, Mercier, Corinne, Sibley, L. David, Cesbron-Delauw, Marie-France
The intracellular parasite Toxoplasma gondii resides within a specialized compartment, the parasitophorous vacuole (PV), that resists fusion with host cell endocytic and lysosomal compartments. The...
Håkansson, Sebastian, Morisaki, Hiroshi, Heuser, John, Sibley, L. David
Toxoplasma gondii is a member of the phylum Apicomplexa, a diverse group of intracellular parasites that share a unique form of gliding motility. Gliding is substrate dependent and occurs without...
Toxoplasma gondii Uses Sulfated Proteoglycans for Substrate and Host Cell Attachment
Carruthers, Vern B., Håkansson, Sebastian, Giddings, Olivia K., Sibley, L. David
Toxoplasma gondii is an obligate intracellular parasite that actively invades a wide variety of vertebrate cells, although the basis of this pervasive cell recognition is not understood. We...
Manger, Ian D., Hehl, Adrian, Parmley, Steve, Sibley, L. David, Marra, Marco, Hillier, Ladeana, ...
Toxoplasma gondii is a protozoan parasite responsible for widespread infections in humans and animals. Two major asexual forms are produced during the life cycle of this parasite: the rapidly...
Targeted Disruption of the GRA2 Locus in Toxoplasma gondii Decreases Acute Virulence in Mice
Mercier, Corinne, Howe, Daniel K., Mordue, Dana, Lingnau, Maren, Sibley, L. David
Following invasion into the host cell, the protozoan Toxoplasma gondii secretes a variety of proteins that modify the parasitophorous vacuole. Within the vacuole, the 28-kDa dense granule protein...
Howe, Daniel K., Crawford, Amy C., Lindsay, David, Sibley, L. David
Neospora caninum is an apicomplexan parasite that is closely related to Toxoplasma gondii and has been found to be associated with neurological disorders in dogs and congenital infections and...
Biogenesis of Nanotubular Network in Toxoplasma Parasitophorous Vacuole Induced by Parasite Proteins
Mercier, Corinne, Dubremetz, Jean-François, Rauscher, Béatrice, Lecordier, Laurence, Sibley, L. David, Cesbron-Delauw, Marie-France
The intracellular parasite Toxoplasma gondii develops within a nonfusogenic vacuole containing a network of elongated nanotubules that form connections with the vacuolar membrane. Parasite secretory...
Howe, Daniel K., Tang, Keliang, Conrad, Patricia A., Sverlow, Karen, Dubey, J. P., Sibley, L. David
Neosporosis is an economically important disease of dairy cattle caused by the protozoan Neospora caninum. Diagnostic tests for neosporosis are complicated by the potential for cross-reaction of...
Cytoskeleton of Apicomplexan Parasites
Morrissette, Naomi S., Sibley, L. David
The Apicomplexa are a phylum of diverse obligate intracellular parasites including Plasmodium spp., the cause of malaria; Toxoplasma gondii and Cryptosporidium parvum, opportunistic pathogens of...
Identification of quantitative trait loci controlling acute virulence in Toxoplasma gondii
Su, Chunlei, Howe, Daniel K., Dubey, J. P., Ajioka, James W., Sibley, L. David
Strains of Toxoplasma gondii can be grouped into three predominant clonal lineages with members of the type I group being uniformly lethal in mice. To elucidate the basis of this extreme virulence, a...
Asai, Takashi, Takeuchi, Tsutomu, Diffenderfer, Jeff, Sibley, L. David
Approximately 150,000 small-molecule compounds were tested by a robotic screening assay for their ability to inhibit nucleoside triphosphate hydrolase (NTPase), a novel enzyme of the tachyzoite form...
Toxoplasma evacuoles: a two-step process of secretion and fusion forms the parasitophorous vacuole
Håkansson, Sebastian, Charron, Audra J., Sibley, L.David
Rapid discharge of secretory organelles called rhoptries is tightly coupled with host cell entry by the protozoan parasite Toxoplasma gondii. Rhoptry contents were deposited in clusters of vesicles...
Huynh, My-Hang, Rabenau, Karen E., Harper, Jill M., Beatty, Wandy L., Sibley, L.David, Carruthers, Vern B.
Vertebrate cells are highly susceptible to infection by obligate intracellular parasites such as Toxoplasma gondii, yet the mechanism by which these microbes breach the confines of their target cell...
ApiEST-DB: analyzing clustered EST data of the apicomplexan parasites
Li, Li, Crabtree, Jonathan, Fischer, Steve, Pinney, Deborah, Stoeckert, Christian J., Sibley, L. David, ...
ApiEST-DB (http://www.cbil.upenn.edu/paradbs-servlet/) provides integrated access to publicly available EST data from protozoan parasites in the phylum Apicomplexa. The database currently...
Dinitroanilines Bind α-Tubulin to Disrupt Microtubules
Morrissette, Naomi S., Mitra, Arpita, Sept, David, Sibley, L. David
Protozoan parasites are remarkably sensitive to dinitroanilines such as oryzalin, which disrupt plant but not animal microtubules. To explore the basis of dinitroaniline action, we isolated 49...
Li, Li, Brunk, Brian P., Kissinger, Jessica C., Pape, Deana, Tang, Keliang, Cole, Robert H., ...
Large-scale EST sequencing projects for several important parasites within the phylum Apicomplexa were undertaken for the purpose of gene discovery. Included were several parasites of medical...
Brossier, Fabien, Jewett, Travis J., Sibley, L. David, Urban, Sinisa
Apicomplexan parasites cause serious human and animal diseases, the treatment of which requires identification of new therapeutic targets. Host-cell invasion culminates in the essential cleavage of...
Khan, Asis, Taylor, Sonya, Su, Chunlei, Mackey, Aaron J., Boyle, Jon, Cole, Robert, ...
Toxoplasma gondii is a highly successful protozoan parasite in the phylum Apicomplexa, which contains numerous animal and human pathogens. T.gondii is amenable to cellular, biochemical, molecular and...
Gliding Motility Leads to Active Cellular Invasion by Cryptosporidium parvum Sporozoites
Wetzel, Dawn M., Schmidt, Joann, Kuhlenschmidt, Mark S., Dubey, J. P., Sibley, L. David
We examined gliding motility and cell invasion by an early-branching apicomplexan, Cryptosporidium parvum, which causes diarrheal disease in humans and animals. Real-time video microscopy...
Khan, A., Su, C., German, M., Storch, G. A., Clifford, D. B., Sibley, L. David
Toxoplasma gondii is an important food- and waterborne opportunistic pathogen that causes severe disease in immunocompromised patients. T. gondii has an unusual clonal population structure consisting...
Sahoo, Nivedita, Beatty, Wandy, Heuser, John, Sept, David, Sibley, L. David
Toxoplasma is a protozoan parasite in the phylum Apicomplexa, which contains a number of medically important parasites that rely on a highly unusual form of motility termed gliding to actively...
Nowakowska, Dorota, Colón, Iris, Remington, Jack S., Grigg, Michael, Golab, Elzbieta, Wilczynski, J., ...
Toxoplasma gondii has a clonal population genetic structure with three (I, II, and III) lineages that predominate in North America and Europe. Type II strains cause most cases of symptomatic human...
Boyle, Jon P., Rajasekar, Badri, Saeij, Jeroen P. J., Ajioka, James W., Berriman, Matthew, Paulsen, Ian, ...
Toxoplasma gondii, an obligate intracellular protozoan of the phylum Apicomplexa, is estimated to infect over a billion people worldwide as well as a great many other mammalian and avian hosts....
Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii
Khan, Asis, Böhme, Ulrike, Kelly, Krystyna A., Adlem, Ellen, Brooks, Karen, Simmonds, Mark, ...
Toxoplasma gondii is a globally distributed protozoan parasite that can infect virtually all warm-blooded animals and humans. Despite the existence of a sexual phase in the life cycle, T. gondii has...
Genetic approaches to studying virulence and pathogenesis in Toxoplasma gondii.
Sibley, L David, Mordue, Dana G, Su, Chunlei, Robben, Paul M, Howe, Dan K
Toxoplasma gondii is a common protozoan parasite that causes disease in immunocompromised humans. Equipped with a wide array of experimental tools, T. gondii has rapidly developed as a model parasite...
Fux, Blima, Nawas, Julie, Khan, Asis, Gill, Darcy B., Su, Chunlei, Sibley, L. David
Toxoplasma gondii undergoes differentiation from rapidly growing tachyzoites to slowly growing bradyzoites during its life cycle in the intermediate host, and conversion can be induced in vitro by...
Artemisinin-Resistant Mutants of Toxoplasma gondii Have Altered Calcium Homeostasis▿
Nagamune, Kisaburo, Moreno, Silvia N. J., Sibley, L. David
Artemisinin is a plant sesquiterpene lactone that has become an important drug for combating malaria, especially in regions where resistance to other drugs is widespread. While the mechanism of...
Nagamune, Kisaburo, Beatty, Wandy L., Sibley, L. David
Intracellular calcium controls several crucial cellular events in apicomplexan parasites, including protein secretion, motility, and invasion into and egress from host cells. The plant compound...
Recruitment of Gr-1+ monocytes is essential for control of acute toxoplasmosis
Robben, Paul M., LaRegina, Marie, Kuziel, William A., Sibley, L. David
Circulating murine monocytes comprise two largely exclusive subpopulations that are responsible for seeding normal tissues (Gr-1−/CCR2−/CX3CR1high) or responding to sites of inflammation...
Transepithelial Migration of Toxoplasma gondii Is Linked to Parasite Motility and Virulence
Barragan, Antonio, Sibley, L. David
After oral ingestion, Toxoplasma gondii crosses the intestinal epithelium, disseminates into the deep tissues, and traverses biological barriers such as the placenta and the blood-brain barrier to...
Mordue, Dana G., Desai, Naishadh, Dustin, Michael, Sibley, L. David
The protozoan parasite Toxoplasma gondii actively penetrates its host cell by squeezing through a moving junction that forms between the host cell plasma membrane and the parasite. During invasion,...
Brossier, Fabien, Starnes, G. Lucas, Beatty, Wandy L., Sibley, L. David
Rhomboids are serine proteases that cleave their substrates within the transmembrane domain. Toxoplasma gondii contains six rhomboids that are expressed in different life cycle stages and localized...
A Novel Actin-Related Protein Is Associated with Daughter Cell Formation in Toxoplasma gondii▿ †
Gordon, Jennifer L., Beatty, Wandy L., Sibley, L. David
Cell division in Toxoplasma gondii occurs by an unusual budding mechanism termed endodyogeny, during which twin daughters are formed within the body of the mother cell. Cytokinesis begins with the...
Khan, Asis, Taylor, Sonya, Ajioka, James W., Rosenthal, Benjamin M., Sibley, L. David
Pathogenicity differences among laboratory isolates of the dominant clonal North American and European lineages of Toxoplasma gondii are largely controlled by polymorphisms and expression differences...
Plants, endosymbionts and parasites: Abscisic acid and calcium signaling
Nagamune, Kisaburo, Xiong, Liming, Chini, Eduardo, Sibley, L David
It was recently discovered that the protozoan parasite, Toxoplasma gondii produces and uses the plant hormone, abscisic acid (ABA), for communication. Following intracellular replication, ABA...