cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.
Verboomen, H, Wuytack, F, Eggermont, J A, De Jaegere, S, Missiaen, L, Raeymaekers, L, ...
Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino...
Alkalinization stimulates the purified plasma-membrane Ca2+ pump by increasing its Ca2+ affinity.
Missiaen, L, Droogmans, G, De Smedt, H, Wuytack, F, Raeymaekers, L, Casteels, R
The finding that negatively charged phospholipids activate the plasma-membrane (Ca2+ + Mg2+)-ATPase and that polycations counteract this stimulation suggest that negative charges in the environment...
Missiaen, L, Raeymaekers, L, Wuytack, F, Vrolix, M, De Smedt, H, Casteels, R
The aim of the present work was to investigate the stimulation of the plasma-membrane Ca2+-transporting ATPase by negatively charged phospholipids. The Ca2+-transporting ATPase was purified from pig...
Missiaen, L, Raeymaekers, L, Droogmans, G, Wuytack, F, Casteels, R
Negatively charged phospholipids strongly stimulate the purified plasma membrane Ca2+ pump of erythrocytes [Enyedi, Flura, Sarkadi, Gardos & Carafoli (1987) J. Biol. Chem. 262, 6425-6430] and of...
Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.
Wuytack, F, Eggermont, J A, Raeymaekers, L, Plessers, L, Casteels, R
We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2...
Vrolix, M, Raeymaekers, L, Wuytack, F, Hofmann, F, Casteels, R
The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase...
Wuytack, F, Kanmura, Y, Eggermont, J A, Raeymaekers, L, Verbist, J, Hartweg, D, ...
Smooth muscle expresses in its endoplasmic reticulum an isoform of the Ca2+-transport ATPase that is very similar to or identical with that of the cardiac-muscle/slow-twitch skeletal-muscle form....
Volume-activated chloride currents are not correlated with P-glycoprotein expression.
De Greef, C, Sehrer, J, Viana, F, Van Acker, K, Eggermont, J, Mertens, L, ...
It has been proposed that P-glycoprotein, the product of the human MDR1 gene, may function not only as a drug transporter but, depending on the conditions, as a volume-activated Cl- channel...
Missiaen, L, Wuytack, F, Raeymaekers, L, De Smedt, H, Casteels, R
We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin....
Evidence for the presence in smooth muscle of two types of Ca2+-transport ATPase.
Wuytack, F, Raeymaekers, L, Verbist, J, De Smedt, H, Casteels, R
Membrane fractions prepared from smooth muscle of the pig stomach (antral part) contain two Ca2+-dependent phosphoprotein intermediates belonging to different Ca2+-transport ATPases. These...
Verbist, J, Wuytack, F, Raeymaekers, L, Van Leuven, F, Cassiman, J J, Casteels, R
A monoclonal antibody (2B3) directed against the calmodulin-binding (Ca2+ + Mg2+)-dependent ATPase from pig stomach smooth muscle was prepared. This antibody reacts with a 130,000-Mr protein that...
Raeymaekers, L, Hofmann, F, Casteels, R
Phospholamban of isolated sarcoplasmic reticulum of cardiac and smooth muscle is phosphorylated by cyclic GMP-dependent protein kinase (G-kinase). Concomitantly, the affinity of the Ca2+ pump for...
Verbist, J, Wuytack, F, Raeymaekers, L, Casteels, R
Antibodies directed against the purified calmodulin-binding (Ca2+ + Mg2+)-ATPase [(Ca2+ + Mg2+)-dependent ATPase] from pig erythrocytes and from smooth muscle of pig stomach (antral part) were raised...
Raeymaekers, L, Wuytack, F, Eggermont, J, De Schutter, G, Casteels, R
1. A plasma-membrane fraction was isolated from the smooth muscle of the pig stomach by using differential and sucrose-density-gradient centrifugations. When the centrifugation was carried out after...
Missiaen, L, De Smedt, H, Parys, J B, Raeymaekers, L, Droogmans, G, ...
We have investigated the detailed kinetics of the passive Ca2+ leak from non-mitochondrial Ca2+ stores in permeabilized A7r5 cells. The decrease in the content of stored Ca2+ in the presence of 2...
Dode, L, Wuytack, F, Kools, P F, Baba-Aissa, F, Raeymaekers, L, Briké, F, ...
cDNA and genomic clones encoding human sarco/endoplasmic reticulum Ca(2+)-ATPase 3 (SERCA3) were isolated. The composite nucleotide sequence of the 4.6 kb cDNA, as well as the partial structure of 25...
Missiaen, L, Declerck, I, Droogmans, G, Plessers, L, De Smedt, H, Raeymaekers, L, ...
1. The properties of intracellular Ca2+ stores of intact- and of saponin-skinned A7r5 (an established cell line from embryonic rat aorta) smooth muscle cells were studied by measuring 45Ca2+ and...
1. The role of an intracellular Ca store in excitation-contraction coupling was studied by recording isometric tension development of thin strips (100-150 micron in diameter) of taenia coli incubated...
cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.
Verboomen, H, Wuytack, F, Eggermont, J A, De Jaegere, S, Missiaen, L, Raeymaekers, L, ...
Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino...
Alkalinization stimulates the purified plasma-membrane Ca2+ pump by increasing its Ca2+ affinity.
Missiaen, L, Droogmans, G, De Smedt, H, Wuytack, F, Raeymaekers, L, Casteels, R
The finding that negatively charged phospholipids activate the plasma-membrane (Ca2+ + Mg2+)-ATPase and that polycations counteract this stimulation suggest that negative charges in the environment...
Missiaen, L, Raeymaekers, L, Wuytack, F, Vrolix, M, De Smedt, H, Casteels, R
The aim of the present work was to investigate the stimulation of the plasma-membrane Ca2+-transporting ATPase by negatively charged phospholipids. The Ca2+-transporting ATPase was purified from pig...
Missiaen, L, Raeymaekers, L, Droogmans, G, Wuytack, F, Casteels, R
Negatively charged phospholipids strongly stimulate the purified plasma membrane Ca2+ pump of erythrocytes [Enyedi, Flura, Sarkadi, Gardos & Carafoli (1987) J. Biol. Chem. 262, 6425-6430] and of...
Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.
Wuytack, F, Eggermont, J A, Raeymaekers, L, Plessers, L, Casteels, R
We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2...
Vrolix, M, Raeymaekers, L, Wuytack, F, Hofmann, F, Casteels, R
The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase...
Wuytack, F, Kanmura, Y, Eggermont, J A, Raeymaekers, L, Verbist, J, Hartweg, D, ...
Smooth muscle expresses in its endoplasmic reticulum an isoform of the Ca2+-transport ATPase that is very similar to or identical with that of the cardiac-muscle/slow-twitch skeletal-muscle form....
Volume-activated chloride currents are not correlated with P-glycoprotein expression.
De Greef, C, Sehrer, J, Viana, F, Van Acker, K, Eggermont, J, Mertens, L, ...
It has been proposed that P-glycoprotein, the product of the human MDR1 gene, may function not only as a drug transporter but, depending on the conditions, as a volume-activated Cl- channel...
Missiaen, L, Wuytack, F, Raeymaekers, L, De Smedt, H, Casteels, R
We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin....
Evidence for the presence in smooth muscle of two types of Ca2+-transport ATPase.
Wuytack, F, Raeymaekers, L, Verbist, J, De Smedt, H, Casteels, R
Membrane fractions prepared from smooth muscle of the pig stomach (antral part) contain two Ca2+-dependent phosphoprotein intermediates belonging to different Ca2+-transport ATPases. These...
Verbist, J, Wuytack, F, Raeymaekers, L, Van Leuven, F, Cassiman, J J, Casteels, R
A monoclonal antibody (2B3) directed against the calmodulin-binding (Ca2+ + Mg2+)-dependent ATPase from pig stomach smooth muscle was prepared. This antibody reacts with a 130,000-Mr protein that...
Raeymaekers, L, Hofmann, F, Casteels, R
Phospholamban of isolated sarcoplasmic reticulum of cardiac and smooth muscle is phosphorylated by cyclic GMP-dependent protein kinase (G-kinase). Concomitantly, the affinity of the Ca2+ pump for...
Verbist, J, Wuytack, F, Raeymaekers, L, Casteels, R
Antibodies directed against the purified calmodulin-binding (Ca2+ + Mg2+)-ATPase [(Ca2+ + Mg2+)-dependent ATPase] from pig erythrocytes and from smooth muscle of pig stomach (antral part) were raised...
Raeymaekers, L, Wuytack, F, Eggermont, J, De Schutter, G, Casteels, R
1. A plasma-membrane fraction was isolated from the smooth muscle of the pig stomach by using differential and sucrose-density-gradient centrifugations. When the centrifugation was carried out after...
Missiaen, L, Declerck, I, Droogmans, G, Plessers, L, De Smedt, H, Raeymaekers, L, ...
1. The properties of intracellular Ca2+ stores of intact- and of saponin-skinned A7r5 (an established cell line from embryonic rat aorta) smooth muscle cells were studied by measuring 45Ca2+ and...
Missiaen, L, De Smedt, H, Parys, J B, Raeymaekers, L, Droogmans, G, ...
We have investigated the detailed kinetics of the passive Ca2+ leak from non-mitochondrial Ca2+ stores in permeabilized A7r5 cells. The decrease in the content of stored Ca2+ in the presence of 2...
Dode, L, Wuytack, F, Kools, P F, Baba-Aissa, F, Raeymaekers, L, Briké, F, ...
cDNA and genomic clones encoding human sarco/endoplasmic reticulum Ca(2+)-ATPase 3 (SERCA3) were isolated. The composite nucleotide sequence of the 4.6 kb cDNA, as well as the partial structure of 25...
1. The role of an intracellular Ca store in excitation-contraction coupling was studied by recording isometric tension development of thin strips (100-150 micron in diameter) of taenia coli incubated...
Macianskiene, R, Bito, V, Raeymaekers, L, Brandts, B, Sipido, K R, Mubagwa, K
We have studied the acute cardiac electrophysiological effects of KB130015 (KB), a drug structurally related to amiodarone. Membrane currents and action potentials were measured at room temperature...