Cuadros-Inostroza, Álvaro, Caldana, Camila, Redestig, Henning, Kusano, Miyako, Lisec, Jan, Peña-Cortés, Hugo, ...
Abstract Background Metabolite profiling, the simultaneous quantification of multiple metabolites in an experiment, is becoming increasingly popular, particularly with the rise of systems-level...
Gas chromatography mass spectrometry–based metabolite profiling in plants (2006)
Jan Lisec, Nicolas Schauer, Joachim Kopka, Lothar Willmitzer, Alisdair R Fernie
Junker, Bjoern H., Wuttke, Rene, Nunes-Nesi, Adriano, Steinhauser, Dirk, Schauer, Nicolas, Büssis, Dirk, ...
Modification of tuber carbohydrate metabolism by the tuber-specific expression of a yeast invertase targeted to the cytosol or apoplast has previously been demonstrated to have diverse effects on...
Junker, Bjoern H., Wuttke, Rene, Nunes-Nesi, Adriano, Steinhauser, Dirk, Schauer, Nicolas, Büssis, Dirk, ...
Modification of tuber carbohydrate metabolism by the tuber-specific expression of a yeast invertase targeted to the cytosol or apoplast has previously been demonstrated to have diverse effects on...
GMD@CSB.DB: the Golm Metabolome Database (2005)
Joachim Kopka, Nicolas Schauer, Stephan Krueger, Claudia Birkemeyer, Björn Usadel, Eveline Bergmüller, ...
Summary: Metabolomics, in particular gas chromatography–mass spectrometry (GC–MS) based metabolite profiling of biological extracts, is rapidly becoming one of the cornerstones of functional...
Nikiforova, Victoria J., Daub, Carsten O., Hesse, Holger, Willmitzer, Lothar, Hoefgen, Rainer
The systematic accumulation of gene expression data, although revolutionary, is insufficient in itself for an understanding of system-level physiology. In the post-genomic era, the next cognitive...
Nikiforova, Victoria J., Daub, Carsten O., Hesse, Holger, Willmitzer, Lothar, Hoefgen, Rainer
The systematic accumulation of gene expression data, although revolutionary, is insufficient in itself for an understanding of system-level physiology. In the post-genomic era, the next cognitive...
Lytovchenko, Anna, Schauer, Nicolas, Willmitzer, Lothar, Fernie, Alisdair R.
Constitutive antisense inhibition of the cytosolic isoform of phosphoglucomutase in the potato (Solanum tuberosum L.) results in restriction of photosynthesis, growth inhibition and modified tuber...
Davies, Howard V., Shepherd, Louise V. T., Burrell, Michael M., Carrari, Fernando, Urbanczyk-Wochniak, Ewa, Leisse, Andrea, ...
Potato plants (Solanum tuberosum L. cvs. Desiree and Record) transformed with sense and antisense constructs of a cDNA encoding the potato fructokinase StFK1 exhibited altered transcription of this...
Junker, Bjoern H., Wuttke, Rene, Nunes-Nesi, Adriano, Steinhauser, Dirk, Schauer, Nicolas, Büssis, Dirk, ...
Modification of tuber carbohydrate metabolism by the tuber-specific expression of a yeast invertase targeted to the cytosol or apoplast has previously been demonstrated to have diverse effects on...
Davies, Howard V., Shepherd, Louise V. T., Burrell, Michael M., Carrari, Fernando, Urbanczyk-Wochniak, Ewa, Leisse, Andrea, ...
Potato plants (Solanum tuberosum L. cvs Desiree and Record) transformed with sense and antisense constructs of a cDNA encoding the potato fructokinase StFK1 exhibited altered transcription of this...
Lytovchenko, Anna, Schauer, Nicolas, Willmitzer, Lothar, Fernie, Alisdair R.
Constitutive antisense inhibition of the cytosolic isoform of phosphoglucomutase in the potato (Solanum tuberosum L.) results in restriction of photosynthesis, growth inhibition and modified tuber...
GMD@CSB.DB: the Golm Metabolome Database (2005)
Kopka, Joachim, Schauer, Nicolas, Krueger, Stephan, Birkemeyer, Claudia, Usadel, Björn, Bergmüller, Eveline, ...
Summary: Metabolomics, in particular gas chromatography–mass spectrometry (GC–MS) based metabolite profiling of biological extracts, is rapidly becoming one of the cornerstones of functional...
Nikiforova, Victoria J., Daub, Carsten O., Hesse, Holger, Willmitzer, Lothar, Hoefgen, Rainer
The systematic accumulation of gene expression data, although revolutionary, is insufficient in itself for an understanding of system-level physiology. In the post-genomic era, the next cognitive...
Lytovchenko, Anna, Schauer, Nicolas, Willmitzer, Lothar, Fernie, Alisdair R.
Constitutive antisense inhibition of the cytosolic isoform of phosphoglucomutase in the potato (Solanum tuberosum L.) results in restriction of photosynthesis, growth inhibition and modified tuber...
Davies, Howard V., Shepherd, Louise V. T., Burrell, Michael M., Carrari, Fernando, Urbanczyk-Wochniak, Ewa, Leisse, Andrea, ...
Potato plants (Solanum tuberosum L. cvs. Desiree and Record) transformed with sense and antisense constructs of a cDNA encoding the potato fructokinase StFK1 exhibited altered transcription of this...
Junker, Bjoern H., Wuttke, Rene, Nunes-Nesi, Adriano, Steinhauser, Dirk, Schauer, Nicolas, Büssis, Dirk, ...
Modification of tuber carbohydrate metabolism by the tuber-specific expression of a yeast invertase targeted to the cytosol or apoplast has previously been demonstrated to have diverse effects on...
GMD@CSB.DB: the Golm metabolome database (2004)
Kopka, Joachim, Schauer, Nicolas, Krueger, Stephan, Birkemeyer, Claudia, Usadel, Björn, Bergmüller, Eveline, ...
Summary: Metabolomics, in particular gas chromatography - mass spectrometry (GC-MS) based metabolite profiling of biological extracts, is rapidly becoming one of the cornerstones of functional...
Nikiforova, Victoria J., Gakière, Bertrand, Kempa, Stefan, Adamik, Monika, Willmitzer, Lothar, Hesse, Holger, ...
A genomics analysis on sulphur metabolism has been conducted at the level of transcriptomics and metabolomics. The analysis of these data after applying bioinformatic tools is to reveal novel...
Nikiforova, Victoria J., Gakière, Bertrand, Kempa, Stefan, Adamik, Monika, Willmitzer, Lothar, Hesse, Holger, ...
A genomics analysis on sulphur metabolism has been conducted at the level of transcriptomics and metabolomics. The analysis of these data after applying bioinformatic tools is to reveal novel...
Fisahn, Joachim, Herde, Oliver, Willmitzer, Lothar, Peña-Cortés, Hugo
Plants respond to various abiotic stimuli by activation and propagation of fast electrical signals, action potentials. To resolve the temporal increase in cytosolic Ca2+ during the action...
GMD@CSB.DB: the Golm metabolome database (2004)
Kopka, Joachim, Schauer, Nicolas, Krueger, Stephan, Birkemeyer, Claudia, Usadel, Björn, Bergmüller, Eveline, ...
Summary: Metabolomics, in particular gas chromatography - mass spectrometry (GC-MS) based metabolite profiling of biological extracts, is rapidly becoming one of the cornerstones of functional...
Nikiforova, Victoria J., Gakière, Bertrand, Kempa, Stefan, Adamik, Monika, Willmitzer, Lothar, Hesse, Holger, ...
A genomics analysis on sulphur metabolism has been conducted at the level of transcriptomics and metabolomics. The analysis of these data after applying bioinformatic tools is to reveal novel...
Parallel analysis of transcript and metabolic profiles: a new approach in systems biology (2003)
Ewa Urbanczyk-Wochniak, Alexander Luedemann, Joachim Kopka, Joachim Selbig, Ute Roessner-Tunali, Lothar Willmitzer, ...
Urbanczyk-Wochniak, Ewa, Leisse, Andrea, Roessner-Tunali, Ute, Lytovchenko, Anna, Reismeier, Jörg, Willmitzer, Lothar, ...
Here we investigate the role of hexoses in the metabolism of the developing potato (Solanum tuberosum) tuber by the expression of a bacterial xylose isomerase which catalyzes the interconversion of...
A yeast-based model system for cloning secreted and membrane proteins (2002)
SURPILI,MARCELO J., MÜLLER-RÖBER,BERND, WILLMITZER,LOTHAR
The targeting of proteins to cell organelles and membranes, or of proteins destined to secretion, is coordinated by signal sequences located at the 5´-end of their respective genes. A signal...
Regierer,Babette, Fernie,Alisdair R., Springer,Franziska, Perez-Melis,Alicia, Leisse,Andrea, Koehl,Karin, ...
Starch represents the most important carbohydrate used for food and feed purposes. With the aim of increasing starch content, we decided to modulate the adenylate pool by changing the activity of the...
Regierer, Babette, Fernie, Alisdair R., Springer, Franziska, Perez-Melis, Alicia, Leisse, Andrea, Koehl, Karin, ...
Starch represents the most important carbohydrate used for food and feed purposes. With the aim of increasing starch content, we decided to modulate the adenylate pool by changing the activity of the...
Acceleration of potato tuber sprouting by the expression of a bacterial pyrophosphatase (2001)
Eva M. Farré, Antje Bachmann, Lothar Willmitzer, Richard N. Trethewey
Metabolite profiling for plant functional genomics (2000)
Oliver Fiehn, Joachim Kopka, Peter Dörmann, Thomas Altmann, Richard N. Trethewey, Lothar Willmitzer
Hajirezaei, Mohammad-Reza, Takahata, Yasuhiro, Trethewey, Richard N., Willmitzer, Lothar, Sonnewald, Uwe
During tuberization in Solanum tuberosum var. Desirée maximal catalytic activities of invertase(s) and sucrose synthase are inversely correlated. During the early stages, invertase...
Increased potato tuber size resulting from apoplastic expression of a yeast invertase (1997)
Uwe Sonnewald, Mohammad-Reza Hajirezaei, Jens Kossmann, Arnd Heyer, Richard N. Trethewey, Lothar Willmitzer
Structure and Expression of Mitochondrial Citrate Synthases from Higher Plants (1996)
La Cognata, Ursula, Landschütze, Volker, Willmitzer, Lothar, Müller-Röber, Bernd
Mitochondrial citrate synthase (EC 4.1.3.7) represents the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA....
Characterization of waldmeister, a novel developmental mutant in Arabidopsis thaliana (1996)
Felix, Gisela, Altmann, Thomas, Uwer, Ursula, Jessop, Alison, Willmitzer, Lothar, Morris, Peter-Christian
A novel Arabidopsis thaliana (L.) Heynh. developmental mutant, waldmeister (wam), is described. This mutant was found in the progeny arising from an Ac-Ds tagging experiment, but does not appear to...
Leidreiter, Kirsten, Heineke, Dieter, Heldt, Hans W., Müller-Röber, Bernd, Sonnewald, Uwe, Willmitzer, Lothar
In an attempt to study the importance of starch synthesis in leaves with respect to sink-source interactions, we investigated daily turnover of carbohydrates in leaves of transgenic potato plants...
Translatability of a plant-mRNA strongly influences its accumulation in transgenic plants (1990)
Vancanneyt, Guy, Rosahl, Sabine, Willmitzer, Lothar
Current knowledge of parameters affecting RNA stability is very restricted in plants. Here we investigated factors which might contribute to the stability of a particular plant messenger RNA. To this...
Stockhaus, Jorg, Eckes, Peter, Blau, Astrid, Schell, Jeff, Willmitzer, Lothar
ST-LS1, a single copy gene from potato displaying a leaf/stem specific gene expression, was tagged by an exon modification and introduced into both potato and tobacco cells using Agrobacteri-um...
Primary structure of a proteinase inhibitor II gene from potato (Solanum tuberosum) (1986)
Keil, Michael, Sanchez-Serrano, Jose, Schell, Jeff, Willmitzer, Lothar
The isolation and characterization of a genomic clone encoding proteinase inhibitor II of potato (Solanum tuberoaum is described. The structure of this gene was determined by sequencing a genomic...
Henri De Greve, Jan Leemans, Jean-Pierre Hernalsteens, Lin Thia-Toong, Marc De Beuckeleer, Lothar Willmitzer, ...
Braunschweig, Techn. Univ., Naturwiss. Fak., Diss., 1977.
Willmitzer, Lothar, Bode, Jürgen, Wagner, Karl G.
cipher on a molecular level the role of protamine phos-phorylation in spermiogenesis, clupeine Z species containing one, two or three serine phosphates were prepared utilizing a recently developed...
Willmitzer, Lothar, Bode, Jürgen, Wagner, Karl G.
The influence of protamine phosphorylation upon the conformation of nucleoprotamine complexes was studied at different ionic strengths using circular dichroism. The sharp onset of CD spectral changes...
Application of fluorescamine to the study of protein-DNA interactions (1975)
Bode, Jürgen, Willmitzer, Lothar
The reactivity of α-amino groups of basic proteins towards fluorescamine is essentially abolished if salt linkages with DNA phosphate groups are formed. This observation prompted the elaboration of...
Hellwege, Elke M., Czapla, Sylvia, Jahnke, Anuschka, Willmitzer, Lothar, Heyer, Arnd G.
The ability to synthesize high molecular weight inulin was transferred to potato plants via constitutive expression of the 1-SST (sucrose:sucrose 1-fructosyltransferase) and the 1-FFT (fructan:...
Herde, Oliver, Peña Cortés, Hugo, Wasternack, Claus, Willmitzer, Lothar, Fisahn, Joachim
Experiments were performed on three abscisic acid (ABA)-deficient tomato (Lycopersicon esculentum Mill.) mutants, notabilis, flacca, and sitiens, to investigate the role of ABA and jasmonic acid (JA)...
Farré, Eva M., Geigenberger, Peter, Willmitzer, Lothar, Trethewey, Richard N.
The early stages of tuber development are characterized by cell division, high metabolic activity, and the predominance of invertase as the sucrose (Suc) cleaving activity. However, during the...
Veramendi, Jon, Roessner, Ute, Renz, Andreas, Willmitzer, Lothar, Trethewey, Richard N.
Potato (Solanum tuberosum L.) plants transformed with sense and antisense constructs of a cDNA encoding the potato hexokinase 1 (StHK1) exhibited altered enzyme activities and expression of StHK1...
Rehm, Jochen, Willmitzer, Lothar, Heyer, Arnd G.
Sucrose-inducible secretory sucrose:sucrose 1-fructosyltransferase (1-SST) from Aspergillus foetidus has been purified and subjected to N-terminal amino acid sequence determination. The enzyme is...
Farré, Eva M., Tiessen, Axel, Roessner, Ute, Geigenberger, Peter, Trethewey, Richard N., Willmitzer, Lothar
The compartmentation of metabolism in heterotrophic plant tissues is poorly understood due to the lack of data on metabolite distributions and fluxes between subcellular organelles. The main reason...
Roessner, Ute, Willmitzer, Lothar, Fernie, Alisdair R.
We conducted a comprehensive metabolic phenotyping of potato (Solanum tuberosum L. cv Desiree) tuber tissue that had been modified either by transgenesis or exposure to different environmental...
Zeh, Michaela, Casazza, Anna Paola, Kreft, Oliver, Roessner, Ute, Bieberich, Katrin, Willmitzer, Lothar, ...
Methionine (Met) and threonine (Thr) are members of the aspartate family of amino acids. In plants, their biosynthetic pathways diverge at the level of O-phosphohomo-serine (Ser). The enzymes...
The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato1
Bucher, Marcel, Brunner, Silvia, Zimmermann, Philip, Zardi, Gerardo I., Amrhein, Nikolaus, Willmitzer, Lothar, ...
Bohmert, Karen, Balbo, Ilse, Steinbüchel, Alexander, Tischendorf, Gilbert, Willmitzer, Lothar
Polyhydroxybutyrate (PHB) is a member of a class of thermoelastic polymers called polyhydroxyalkanoates that serve many bacteria as intracellular storage molecules for carbon and energy. Transgenic...
Kochevenko, Andrej, Willmitzer, Lothar
Single amino acid substitutions at either of two crucial positions in acetolactate synthase (ALS) result in a chlorsulfuron-insensitive form of this enzyme and, as a consequence, a...
Production of Human Papillomavirus Type 16 Virus-Like Particles in Transgenic Plants
Biemelt, Sophia, Sonnewald, Uwe, Galmbacher, Petra, Willmitzer, Lothar, Müller, Martin
Cervical cancer is linked to infection with human papillomaviruses (HPV) and is the third most common cancer among women worldwide. There is a strong demand for the development of an HPV preventive...
De Novo Amino Acid Biosynthesis in Potato Tubers Is Regulated by Sucrose Levels1[w]
Roessner-Tunali, Ute, Urbanczyk-Wochniak, Ewa, Czechowski, Tomasz, Kolbe, Anna, Willmitzer, Lothar, Fernie, Alisdair R.
Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to...
Pēna-Cortés, Hugo, Sánchez-Serrano, José J., Mertens, Rüdiger, Willmitzer, Lothar, Prat, Salomé
Plants respond to wounding or pathogen attack by a variety of biochemical reactions, involving in some instances gene activation in tissues far apart from the actual site of wounding or pathogen...
Stockhaus, Jörg, Eckes, Peter, Rocha-Sosa, Mario, Schell, Jeff, Willmitzer, Lothar
ST-LS1 is a light-inducible, single-copy gene from potato that is expressed only in photosynthetic tissues. Various sequences derived from the 5′-upstream region of this gene were fused to the...
Both developmental and metabolic signals activate the promoter of a class I patatin gene
Rocha-Sosa, Mario, Sonnewald, Uwe, Frommer, Wolf, Stratmann, Marina, Schell, Jeff, Willmitzer, Lothar
Patatin is one of the major soluble proteins in potato tubers and is encoded by a multigene family. Based on structural considerations two classes of patatin genes are distinguished. The...
Keil, Michael, Sánchez-Serrano, Jose J., Willmitzer, Lothar
A chimeric gene consisting of 1.3 kb of the 5' regulatory region of a member of the potato proteinase inhibitor II gene family, the coding region of the bacterial β-glucuronidase (GUS) gene and 260...
The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts
Willmitzer, Lothar, Simons, Gisela, Schell, Jeff
Seven polyadenylated transcripts of significantly different relative abundance were detected in octopine crown-gall tissue after gel electrophoretic separation and subsequent transfer to...
Wound-induced expression of a potato proteinase inhibitor II gene in transgenic tobacco plants
Sanchez-Serrano, Jose J., Keil, Michael, O'Connor, Aileen, Schell, Jeff, Willmitzer, Lothar
A potato proteinase inhibitor II gene was transferred into tobacco plants using Agrobacterium/Ti-plasmid-mediated gene transfer techniques. Whereas no or little expression of the proteinase inhibitor...
Heineke, Dieter, Sonnewald, Uwe, Büssis, Dirk, Günter, Gundula, Leidreiter, Kirsten, Wilke, Ingo, ...
In potato plants (Solanum tuberosum), a chimeric yeast-derived invertase gene fused to a 35S cauliflower mosaic virus promoter has been expressed. The protein was targeted to the cell wall by using...
Parallel analysis of transcript and metabolic profiles: a new approach in systems biology
Urbanczyk-Wochniak, Ewa, Luedemann, Alexander, Kopka, Joachim, Selbig, Joachim, Roessner-Tunali, Ute, Willmitzer, Lothar, ...
The past few years in the medical and biological sciences have been characterized by the advent of systems biology. However, despite the well-known connectivity between the molecules described by...
Hellwege, Elke M., Czapla, Sylvia, Jahnke, Anuschka, Willmitzer, Lothar, Heyer, Arnd G.
The ability to synthesize high molecular weight inulin was transferred to potato plants via constitutive expression of the 1-SST (sucrose:sucrose 1-fructosyltransferase) and the 1-FFT (fructan:...
Herde, Oliver, Peña Cortés, Hugo, Wasternack, Claus, Willmitzer, Lothar, Fisahn, Joachim
Experiments were performed on three abscisic acid (ABA)-deficient tomato (Lycopersicon esculentum Mill.) mutants, notabilis, flacca, and sitiens, to investigate the role of ABA and jasmonic acid (JA)...
Farré, Eva M., Geigenberger, Peter, Willmitzer, Lothar, Trethewey, Richard N.
The early stages of tuber development are characterized by cell division, high metabolic activity, and the predominance of invertase as the sucrose (Suc) cleaving activity. However, during the...
Veramendi, Jon, Roessner, Ute, Renz, Andreas, Willmitzer, Lothar, Trethewey, Richard N.
Potato (Solanum tuberosum L.) plants transformed with sense and antisense constructs of a cDNA encoding the potato hexokinase 1 (StHK1) exhibited altered enzyme activities and expression of StHK1...
Rehm, Jochen, Willmitzer, Lothar, Heyer, Arnd G.
Sucrose-inducible secretory sucrose:sucrose 1-fructosyltransferase (1-SST) from Aspergillus foetidus has been purified and subjected to N-terminal amino acid sequence determination. The enzyme is...
Farré, Eva M., Tiessen, Axel, Roessner, Ute, Geigenberger, Peter, Trethewey, Richard N., Willmitzer, Lothar
The compartmentation of metabolism in heterotrophic plant tissues is poorly understood due to the lack of data on metabolite distributions and fluxes between subcellular organelles. The main reason...
Roessner, Ute, Willmitzer, Lothar, Fernie, Alisdair R.
We conducted a comprehensive metabolic phenotyping of potato (Solanum tuberosum L. cv Desiree) tuber tissue that had been modified either by transgenesis or exposure to different environmental...
Zeh, Michaela, Casazza, Anna Paola, Kreft, Oliver, Roessner, Ute, Bieberich, Katrin, Willmitzer, Lothar, ...
Methionine (Met) and threonine (Thr) are members of the aspartate family of amino acids. In plants, their biosynthetic pathways diverge at the level of O-phosphohomo-serine (Ser). The enzymes...
The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato1
Bucher, Marcel, Brunner, Silvia, Zimmermann, Philip, Zardi, Gerardo I., Amrhein, Nikolaus, Willmitzer, Lothar, ...
Bohmert, Karen, Balbo, Ilse, Steinbüchel, Alexander, Tischendorf, Gilbert, Willmitzer, Lothar
Polyhydroxybutyrate (PHB) is a member of a class of thermoelastic polymers called polyhydroxyalkanoates that serve many bacteria as intracellular storage molecules for carbon and energy. Transgenic...
Kochevenko, Andrej, Willmitzer, Lothar
Single amino acid substitutions at either of two crucial positions in acetolactate synthase (ALS) result in a chlorsulfuron-insensitive form of this enzyme and, as a consequence, a...
Production of Human Papillomavirus Type 16 Virus-Like Particles in Transgenic Plants
Biemelt, Sophia, Sonnewald, Uwe, Galmbacher, Petra, Willmitzer, Lothar, Müller, Martin
Cervical cancer is linked to infection with human papillomaviruses (HPV) and is the third most common cancer among women worldwide. There is a strong demand for the development of an HPV preventive...
De Novo Amino Acid Biosynthesis in Potato Tubers Is Regulated by Sucrose Levels1[w]
Roessner-Tunali, Ute, Urbanczyk-Wochniak, Ewa, Czechowski, Tomasz, Kolbe, Anna, Willmitzer, Lothar, Fernie, Alisdair R.
Plant growth and development are strongly dependent on sink-source interactions. In the majority of plants, sucrose (Suc) is the dominant form in which photo-assimilate is transported from source to...
Pēna-Cortés, Hugo, Sánchez-Serrano, José J., Mertens, Rüdiger, Willmitzer, Lothar, Prat, Salomé
Plants respond to wounding or pathogen attack by a variety of biochemical reactions, involving in some instances gene activation in tissues far apart from the actual site of wounding or pathogen...
Stockhaus, Jörg, Eckes, Peter, Rocha-Sosa, Mario, Schell, Jeff, Willmitzer, Lothar
ST-LS1 is a light-inducible, single-copy gene from potato that is expressed only in photosynthetic tissues. Various sequences derived from the 5′-upstream region of this gene were fused to the...
Both developmental and metabolic signals activate the promoter of a class I patatin gene
Rocha-Sosa, Mario, Sonnewald, Uwe, Frommer, Wolf, Stratmann, Marina, Schell, Jeff, Willmitzer, Lothar
Patatin is one of the major soluble proteins in potato tubers and is encoded by a multigene family. Based on structural considerations two classes of patatin genes are distinguished. The...
Keil, Michael, Sánchez-Serrano, Jose J., Willmitzer, Lothar
A chimeric gene consisting of 1.3 kb of the 5' regulatory region of a member of the potato proteinase inhibitor II gene family, the coding region of the bacterial β-glucuronidase (GUS) gene and 260...
The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts
Willmitzer, Lothar, Simons, Gisela, Schell, Jeff
Seven polyadenylated transcripts of significantly different relative abundance were detected in octopine crown-gall tissue after gel electrophoretic separation and subsequent transfer to...
Wound-induced expression of a potato proteinase inhibitor II gene in transgenic tobacco plants
Sanchez-Serrano, Jose J., Keil, Michael, O'Connor, Aileen, Schell, Jeff, Willmitzer, Lothar
A potato proteinase inhibitor II gene was transferred into tobacco plants using Agrobacterium/Ti-plasmid-mediated gene transfer techniques. Whereas no or little expression of the proteinase inhibitor...
Heineke, Dieter, Sonnewald, Uwe, Büssis, Dirk, Günter, Gundula, Leidreiter, Kirsten, Wilke, Ingo, ...
In potato plants (Solanum tuberosum), a chimeric yeast-derived invertase gene fused to a 35S cauliflower mosaic virus promoter has been expressed. The protein was targeted to the cell wall by using...
Parallel analysis of transcript and metabolic profiles: a new approach in systems biology
Urbanczyk-Wochniak, Ewa, Luedemann, Alexander, Kopka, Joachim, Selbig, Joachim, Roessner-Tunali, Ute, Willmitzer, Lothar, ...
The past few years in the medical and biological sciences have been characterized by the advent of systems biology. However, despite the well-known connectivity between the molecules described by...
The metabolic signature related to high plant growth rate in Arabidopsis thaliana
Meyer, Rhonda C., Steinfath, Matthias, Lisec, Jan, Becher, Martina, Witucka-Wall, Hanna, Törjék, Ottó, ...
The decline of available fossil fuel reserves has triggered world-wide efforts to develop alternative energy sources based on plant biomass. Detailed knowledge of the relations of metabolism and...
Lisec, Jan, Meyer, Rhonda C, Steinfath, Matthias, Redestig, Henning, Becher, Martina, Witucka-Wall, Hanna, ...
Plant growth and development are tightly linked to primary metabolism and are subject to natural variation. In order to obtain an insight into the genetic factors controlling biomass and primary...
Improved Heterosis Prediction by Combining Information on DNA- and Metabolic Markers
Gärtner, Tanja, Steinfath, Matthias, Andorf, Sandra, Lisec, Jan, Meyer, Rhonda C., Altmann, Thomas, ...
Zanor, María Inés, Osorio, Sonia, Nunes-Nesi, Adriano, Carrari, Fernando, Lohse, Marc, Usadel, Björn, ...
It has been previously demonstrated, utilizing intraspecific introgression lines, that Lycopersicum Invertase5 (LIN5), which encodes a cell wall invertase, controls total soluble solids content in...
Farre, Eva M., Fernie, Alisdair R., Willmitzer, Lothar
The expression of a heterologous invertase in potato tubers (Solanum tuberosum) in either the cytosol or apoplast leads to a decrease in total sucrose content and to an increase in glucose. Depending...
Szymanski, Jedrzej, Jozefczuk, Szymon, Nikoloski, Zoran, Selbig, Joachim, Nikiforova, Victoria, Catchpole, Gareth, ...
Afzal, Ahmed J., Natarajan, Aparna, Saini, Navinder, Iqbal, M. Javed, Geisler, Matt, El Shemy, Hany A., ...
Heterodera glycines, the soybean cyst nematode (SCN), causes the most damaging chronic disease of soybean (Glycine max). Host resistance requires the resistance allele at rhg1. Resistance destroys...
Prediction of hybrid biomass in Arabidopsis thaliana by selected parental SNP and metabolic markers
Steinfath, Matthias, Gärtner, Tanja, Lisec, Jan, Meyer, Rhonda C., Altmann, Thomas, Willmitzer, Lothar, ...
A recombinant inbred line (RIL) population, derived from two Arabidopsis thaliana accessions, and the corresponding testcrosses with these two original accessions were used for the development and...
QTL analysis of early stage heterosis for biomass in Arabidopsis
Meyer, Rhonda Christiane, Kusterer, Barbara, Lisec, Jan, Steinfath, Matthias, Becher, Martina, Scharr, Hanno, ...
The main objective of this study was to identify genomic regions involved in biomass heterosis using QTL, generation means, and mode-of-inheritance classification analyses. In a modified North...