M. M. Klerks

Quantification of contamination of lettuce by GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium (2007)

Franz, E., Visser, A.A., Klerks, M.M., Termorshuizen, A.J.

The primary objective of this study was to determine the possibility of internalization of GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium (S. Typhimurium) strains...

Quantification of infection of lettuce by GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium (2007)

Franz, E., Klerks, M.M., Termorshuizen, A.J.

The primary objective of this study was to determine the possibility of internalization of GFP-expressing Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium (S. Typhimurium) strains...

Prevalence of shiga toxin-producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E. coli O157:H7 in manure from organic and low-input conventional dairy farms (2007)

Franz, E., Klerks, M.M., Termorshuizen, A.J.

Quantitative detection of Salmonella enterica and the specific interaction with Lactuca sativa (2007)

Klerks, M.M.

http://library.wur.nl/wda/abstracts/ab4219.html

Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency (2007)

Klerks, M.M., Franz, E., Zijlstra, C.

The availability of knowledge of the route of infection and critical plant and microbe factors influencing the colonization efficiency of plants by human pathogenic bacteria is essential for the...

Physiological and molecular response of Lactuca sativa to colonization by Salmonella enterica serovar Dublin (2007)

Klerks, M.M., Franz, E., Zijlstra, C.

This paper describes the physiological and molecular interactions between the human-pathogenic organism Salmonella enterica serovar Dublin and the commercially available mini Roman lettuce cv....

Comparison of Methods of Extracting Salmonella enterica Serovar Enteritidis DNA from Environmental Substrates and Quantification of Organisms by Using a General Internal Procedural Control (2006)

Klerks, M.M., Zijlstra, C., Donnikov, M.

This paper compares five commercially available DNA extraction methods with respect to DNA extraction efficiency of Salmonella enterica serovar Enteritidis from soil, manure, and compost and uses an...

Pathogenen in de primaire productieketen van bladgroenten (2006)

Franz, E., Klerks, M.M., Termorshuizen, A.J., Bokhorst, J.

Ecology of E. coli O157:H7 in the primary production chain of lettuce and modeling risks (2006)

Franz, E., Termorshuizen, A.J., Klerks, M.M., Semenov, A.V., ...

From single to multiplex detection of plant pathogens: pUMA, a new concept of multiplex detection using microarrays (2005)

Bonants, P.J.M., Schoen, C.D., Szemes, M., Klerks, M.M., ...

Novel molecular and biochemical techniques for quality control and monitoring in the agrofood production chain (2005)

Schoen, C.D., Szemes, M., Bonants, P.J.M., Klerks, M.M., ...

Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157:H7, and introduction of a general internal amplification control (2004)

Klerks, M.M., Zijlstra, C.

The objectives of this study were to compare different real-time PCR-based methods for detection of either Salmonella spp. or E. coli O157:H7 with respect to sensitivity, precision and accuracy. In...

Real-time NASBA for sensitive detection of Strawberry vein banding virus (2004)

Vasková, D., Spak, J., Klerks, M.M., Schoen, C.D., Thompson, J.R., Jelkmann, W.

An assay for the detection of Strawberry vein banding virus (SVBV) in Fragaria spp. based on nucleic acid sequence based amplification (NASBA) and real-time detection using molecular beacons...

Detection and tentative grouping of Strawberry crinkle virus isolates (2004)

Klerks, M.M., Lindner, J.L., Vasková, D., Spak, J., Thompson, J.R., Jelkmann, W., ...

A partial sequence of the putative polymerase (L) protein of Strawberry crinkle virus (SCV), genus Cytorhabdovirus, is described. The virus protein was found to be distantly related to the L protein...

From single to multiplex detection of plant pathogens by micro-arrays (2004)

Bonants, P.J.M., Schoen, C.D., Szemes, M., Klerks, M.M., ...

Multiplex RT-PCR detection of four aphid-borne strawberry viruses in Fragaria spp. in combination with a plant mRNA specific internal control (2003)

Thompson, J.R., Wetzel, S., Klerks, M.M., Vasková, D., Schoen, C.D., Spak, J., ...

The principal aphid-borne viruses infecting Strawberry (Fragaria spp.) Strawberry crinkle virus (SCV), Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV) and Strawberry vein...

Use of Amplidet RNA to control the microbiological quality of agro-food products (2002)

Klerks, M.M., Leone, G.

The use of molecular beacons combined with NASBA for the sensitive detection of Sugarcane yellow leaf virus (2002)

Gonsalves, M.C., Klerks, M.M., Verbeek, M., Vega, J.

Sugarcane yellow leaf virus (ScYLV) is widely distributed in Brazil and other sugarcane producing countries causing significant yield losses. Due to the high incidence of the aphid vector, the virus...

Development of a multiplex AmpliDet RNA assay for simultaneous detection and typing of potato virus Y isolates (2002)

Szemes, M., Klerks, M.M., Schoen, C.D.

A multiplex AmpliDet RNA assay was developed for the specific detection of potato virus Y (PVY), and for the differentiation of the PVYN, PVYO/C strains and the tuber necrotic isolates (PVYNTN). The...

Development of a multiplex AmpliDet RNA assay for simultaneous detection and typing of potato virus Y isolates (2002)

Szemes, M., Klerks, M.M., Schoen, C.D.

A multiplex AmpliDet RNA assay was developed for the specific detection of potato virus Y (PVY), and for the differentiation of the PVY(N), PVY(O/C) strains and the tuber necrotic isolates...

Development of a multiplex AmpliDet RNA for the simultaneous detection of Potato leafroll virus and Potato virus Y in potato tubers (2001)

Klerks, M.M., Leone, G.O.M., Verbeek, M., Schoen, C.D.

A novel isothermal multiplex AmpliDet RNA system is described for the simultaneous amplification and detection of Potato leafroll virus (PLRV) and Potato virus Y (PVY) in seed potatoes. The risk of...

Rapid/sensitive detection of apple stem pitting virus in apple trees through RNA amplification and probing with fluorescent molecular beacons (2001)

Klerks, M.M., Leone, G., Lindner, J.L., Schoen, C.D.

Detection of nematode-transmitted nepoviruses by the novel, one-tube AmpliDet RNA assay (2001)

Klerks, M.M., Schoen, C.D.

Comparison of Methods of Extracting Salmonella enterica Serovar Enteritidis DNA from Environmental Substrates and Quantification of Organisms by Using a General Internal Procedural Control

Klerks, M. M., Zijlstra, C., Donnikov, M.

This paper compares five commercially available DNA extraction methods with respect to DNA extraction efficiency of Salmonella enterica serovar Enteritidis from soil, manure, and compost and uses an...

Comparison of Methods of Extracting Salmonella enterica Serovar Enteritidis DNA from Environmental Substrates and Quantification of Organisms by Using a General Internal Procedural Control

Klerks, M. M., Zijlstra, C., Donnikov, M.

This paper compares five commercially available DNA extraction methods with respect to DNA extraction efficiency of Salmonella enterica serovar Enteritidis from soil, manure, and compost and uses an...

Physiological and Molecular Responses of Lactuca sativa to Colonization by Salmonella enterica Serovar Dublin▿

Klerks, M. M., Franz, E., Zijlstra, C.

This paper describes the physiological and molecular interactions between the human-pathogenic organism Salmonella enterica serovar Dublin and the commercially available mini Roman lettuce cv....