Tightening the knot in phytochrome by single molecule atomic force microscopy (2008)
Bornschloegl, T., Anstrom, D. M., Mey, E., Dzubiella, J., Rief, M., Forest, K. T.
A growing number of proteins have been shown to adopt knotted folds. Yet the biological roles and biophysical properties of these knots remain poorly understood. We have used protein engineering and...
Force spectroscopy of single biomolecules (2002)
Many processes in the body are effected and regulated by highly, specialized protein molecules: These molecules certainly deserve name "biochemical nonomachines". Recent progress in single-molecule...
Rief, M, Gautel, M, Schemmel, A, Gaub, H E
The domains of the giant muscle protein titin (connectin) provide interaction sites for other sarcomeric proteins and fulfill mechanical functions. In this paper we compare the unfolding forces of...
Mechanical stability of single DNA molecules.
Clausen-Schaumann, H, Rief, M, Tolksdorf, C, Gaub, H E
Using a modified atomic force microscope (AFM), individual double-stranded (ds) DNA molecules attached to an AFM tip and a gold surface were overstretched, and the mechanical stability of the DNA...
Influence of Substrate Binding on the Mechanical Stability of Mouse Dihydrofolate Reductase
Junker, J. P., Hell, K., Schlierf, M., Neupert, W., Rief, M.
We investigated the effect of substrate binding on the mechanical stability of mouse dihydrofolate reductase using single-molecule force spectroscopy by atomic force microscopy. We find that under...
Rief, M, Gautel, M, Schemmel, A, Gaub, H E
The domains of the giant muscle protein titin (connectin) provide interaction sites for other sarcomeric proteins and fulfill mechanical functions. In this paper we compare the unfolding forces of...
Mechanical stability of single DNA molecules.
Clausen-Schaumann, H, Rief, M, Tolksdorf, C, Gaub, H E
Using a modified atomic force microscope (AFM), individual double-stranded (ds) DNA molecules attached to an AFM tip and a gold surface were overstretched, and the mechanical stability of the DNA...
Influence of Substrate Binding on the Mechanical Stability of Mouse Dihydrofolate Reductase
Junker, J. P., Hell, K., Schlierf, M., Neupert, W., Rief, M.
We investigated the effect of substrate binding on the mechanical stability of mouse dihydrofolate reductase using single-molecule force spectroscopy by atomic force microscopy. We find that under...