Marc-André Sirard

Real-time monitoring of aRNA production during T7 amplification to prevent the loss of sample representation during microarray hybridization sample preparation (2009)

Gilbert, Isabelle, Scantland, Sara, Dufort, Isabelle, Gordynska, Olga, Labbe, Aurélie, Sirard, Marc-André, ...

Gene expression analysis performed through comparative abundance of transcripts is facing a new challenge with the increasing need to compare samples of known cell number, such as early embryos or...

Identification of Novel and Known Oocyte-Specific Genes Using Complementary DNA Subtraction and Microarray Analysis in Three Different Species 1 (2008)

Maud Vallée, Catherine Gravel, Marie-france Palin, Hélène Reghenas, Paul Stothard, David S. Wishart, ...

The main objective of the present study was to identify novel oocyte-specific genes in three different species: bovine, mouse, and Xenopus laevis. To achieve this goal, two powerful technologies were...

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis (2007)

McGraw, Serge, Morin, Guillaume, Vigneault, Christian, Leclerc, Pierre, Sirard, Marc-André

Abstract Background Various histone acetylases (HATs) play a critical role in the regulation of gene expression, but the precise functions of many of those HATs are still unknown. Here we provide...

Alterations in transcript abundance of bovine oocytes recovered at growth and dominance phases of the first follicular wave (2007)

Ghanem, Nasser, Hölker, Michael, Rings, Franca, Jennen, Danyel, Tholen, Ernst, Sirard, Marc-André, ...

Abstract Background Oocyte developmental competence is highly affected by the phase of ovarian follicular wave. Previous studies have shown that oocytes from subordinate follicles recovered at growth...

Cross-species hybridizations on a multi-species cDNA microarray to identify evolutionarily conserved genes expressed in oocytes (2006)

Vallée, Maud, Robert, Claude, Méthot, Steve, Palin, Marie-France, Sirard, Marc-André

Abstract Background Comparative genomic analysis using cDNA microarray is a new approach and a useful tool to identify important genetic sequences or genes that are conserved throughout evolution....

Identification of Novel and Known Oocyte-Specific Genes Using Complementary DNA Subtraction and Microarray Analysis in Three Different Species1 (2005)

Maud Vallée, Catherine Gravel, Marie-France Palin, Hélène Reghenas, Paul Stothard, David S. Wishart, ...

The main objective of the present study was to identify novel oocyte-specific genes in three different species: bovine, mouse, and Xenopus laevis. To achieve this goal, two powerful technologies were...

Expression of Cyclin B1 Messenger RNA Isoforms and Initiation of Cytoplasmic Polyadenylation in the Bovine Oocyte1 (2005)

Karine Tremblay, Christian Vigneault, Serge McGraw, Marc-André Sirard

Oocytes can synthesize and store maternal mRNA in an inactive translational state until the start of in vitro maturation. Cytoplasmic polyadenylation, driven by 3′-untranslated region (UTR)...

Identification of Porcine Oocyte Proteins That Are Associated with Somatic Cell Nuclei after Co-Incubation1 (2004)

Susan Novak, François Paradis, Christian Savard, Karine Tremblay, Marc-André Sirard

Relatively little is known with respect to the oocyte proteins that are involved in nuclear reprogramming of somatic cells in mammals. The aim of the present study was to use a cell-free incubation...

Transcription Factor Expression Patterns in Bovine In Vitro-Derived Embryos Priorto Maternal-Zygotic Transition1 (2004)

Christian Vigneault, Serge McGraw, Lyne Massicotte, Marc-André Sirard

Maternal-zygotic transition (MZT) is a complex phenomenon characterized by the initiation of transcription in the embryo and the replacement of maternal mRNA with embryonic mRNA. In order for this to...

Quantification of Histone Acetyltransferase and Histone Deacetylase Transcripts During Early Bovine Embryo Development1 (2003)

Serge McGraw, Claude Robert, Lyne Massicotte, Marc-André Sirard

Mammalian oocytes are very unique cells with an unlimited developmental potential. These totipotent cells are able to remove existing gene-expression patterns and to impose new ones. However, genome...

Quantification of Cyclin B1 and p34cdc2 in Bovine Cumulus-Oocyte Complexes and Expression Mapping of Genes Involved in the Cell Cycle by Complementary DNA Macroarrays1 (2002)

Claude Robert, Isabelle Hue, Serge McGraw, Dominic Gagné, Marc-André Sirard

Although high amounts of cyclin B1 mRNA are present in bovine oocytes arrested at the germinal vesicle (GV) stage, the protein is not detectable. Furthermore, there is a depletion of the stored...

Quantification of Housekeeping Transcript Levels During the Development of Bovine Preimplantation Embryos1 (2002)

Claude Robert, Serge McGraw, Lyne Massicotte, Marco Pravetoni, Fulvio Gandolfi, Marc-André Sirard

In mammals, the study of gene expression in the preimplantation embryo has been difficult because the standard procedures used to quantify mRNA generally require large amounts of starting material....

Cross-species hybridizations on a multi-species cDNA microarray to identify evolutionarily conserved genes expressed in oocytes

Vallée, Maud, Robert, Claude, Méthot, Steve, Palin, Marie-France, Sirard, Marc-André

Cross-species hybridizations on a multi-species cDNA microarray to identify evolutionarily conserved genes expressed in oocytes

Vallée, Maud, Robert, Claude, Méthot, Steve, Palin, Marie-France, Sirard, Marc-André

Alterations in transcript abundance of bovine oocytes recovered at growth and dominance phases of the first follicular wave

Ghanem, Nasser, Hölker, Michael, Rings, Franca, Jennen, Danyel, Tholen, Ernst, Sirard, Marc-André, ...

Investigation of MYST4 histone acetyltransferase and its involvement in mammalian gametogenesis

McGraw, Serge, Morin, Guillaume, Vigneault, Christian, Leclerc, Pierre, Sirard, Marc-André

Evaluation of the DG29 test for early detection of pregnancy in cattle

Paré, Julie, Audet-Grenier, Marie-Hélène, Rouillier, Paul, Sirard, Marc-André

The objective of this study was to evaluate a commercial blood test (DG29) for pregnancy in cattle. Compared with ultrasound, the sensitivity of the assay for detecting pregnancy around day 30...

Real-time monitoring of aRNA production during T7 amplification to prevent the loss of sample representation during microarray hybridization sample preparation

Gilbert, Isabelle, Scantland, Sara, Dufort, Isabelle, Gordynska, Olga, Labbe, Aurélie, Sirard, Marc-André, ...

Gene expression analysis performed through comparative abundance of transcripts is facing a new challenge with the increasing need to compare samples of known cell number, such as early embryos or...