Li, Chaoyang, Marrelli, Mauro T., Yan, Guiyun, Jacobs-Lorena, Marcelo
Three transgenic Anopheles stephensi lines were established that strongly inhibit transmission of the mouse malaria parasite Plasmodium berghei. Fitness of the transgenic mosquitoes was assessed...
Shigeto Yoshida, Yohei Shimada, Daisuke Kondoh, Yoshiaki Kouzuma, Anil K. Ghosh, Marcelo Jacobs-Lorena, ...
The midgut environment of anopheline mosquitoes plays an important role in the development of the malaria parasite. Using genetic manipulation of anopheline mosquitoes to change the environment in...
Fitness of anopheline mosquitoes expressing transgenes that inhibit Plasmodium development. (2004)
Moreira, Luciano A, Wang, Jing, Collins, Frank H, Jacobs-Lorena, Marcelo
One potential strategy for the control of malaria and other vector-borne diseases is the introduction into wild vector populations of genetic constructs that reduce vectorial capacity. An important...
Transgenic mosquitoes for malaria control: progresses and challenges (2003)
Moreira,Luciano A., Jacobs-Lorena,Marcelo
Malaria kills millions of people every year and the current strategies to control the disease, such as insecticides and drugs have not been completely efficient. Because of that, novel means to fight...
Interrupting malaria transmission by genetic manipulation of anopheline mosquitoes (2003)
Malaria ranks among the deadliest infectious diseases that kills more than one million persons everyyear. The mosquito is an obligatory vector for malaria transmission. In the mosquito,...
Ghosh, Anil K., Ribolla, Paulo E. M., Jacobs-Lorena, Marcelo
Despite vast efforts and expenditures in the past few decades, malaria continues to kill millions of persons every year, and new approaches for disease control are urgently needed. To complete its...
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes. (2000)
Moreira, Luciano A., Edwards, Marten J., Adhami, Faisal, Jasinskiene, Nijole, James, Anthony A., Jacobs-Lorena, Marcelo
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on...
Shen, Zhicheng, Dimopoulos, George, Kafatos, Fotis C., Jacobs-Lorena, Marcelo
An invertebrate intestinal mucin gene, AgMuc1, was isolated from the malaria vector mosquito Anopheles gambiae. The predicted 122-residue protein consists of a central core of seven repeating TTTTVAP...
Qian, Su, Zhang, Jing-Yu, Kay, Mark A., Jacobs-Lorena, Marcelo
The expression of ribosomal protein (r-protein) genes is uniquely regulated at the translational level during early development of Drosophila. Here we report results of a detailed analysis of the...
Characterization of extraribosomal globin messenger RNA and human histone messenger RNA. (1972)
Vita.
A cell surface mucin specifically expressed in the midgut of the malaria mosquito Anopheles gambiae
Shen, Zhicheng, Dimopoulos, George, Kafatos, Fotis C., Jacobs-Lorena, Marcelo
An invertebrate intestinal mucin gene, AgMuc1, was isolated from the malaria vector mosquito Anopheles gambiae. The predicted 122-residue protein consists of a central core of seven repeating TTTTVAP...
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes
Moreira, Luciano A., Edwards, Marten J., Adhami, Faisal, Jasinskiene, Nijole, James, Anthony A., Jacobs-Lorena, Marcelo
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on...
Ghosh, Anil K., Ribolla, Paulo E. M., Jacobs-Lorena, Marcelo
Despite vast efforts and expenditures in the past few decades, malaria continues to kill millions of persons every year, and new approaches for disease control are urgently needed. To complete its...
Translational regulation of a specific gene during oogenesis and embryogenesis of Drosophila
Fruscoloni, Paolo, Al-Atia, Ghada R., Jacobs-Lorena, Marcelo
Polysomal and postpolysomal mRNAs were prepared from Drosophila egg chambers or embryos of different developmental stages. Cell-free translation of these mRNAs followed by two-dimensional gel...
An immune-responsive serpin, SRPN6, mediates mosquito defense against malaria parasites
Abraham, Eappen G., Pinto, Sofia B., Ghosh, Anil, Vanlandingham, Dana L., Budd, Aidan, Higgs, Stephen, ...
We have functionally analyzed the orthologous SRPN6 genes from Anopheles stephensi and Anopheles gambiae using phylogenetic, molecular, reverse genetic, and cell biological tools. The results...
Complexity of RNA in Eggs of DROSOPHILA MELANOGASTER and MUSCA DOMESTICA
Hough-Evans, Barbara R., Jacobs-Lorena, Marcelo, Cummings, Michael R., Britten, Roy J., Davidson, Eric H.
Comparative measurements are presented of the sequence complexity of the RNA stored in the eggs of two dipteran flies, Musca domestica and Drosophila melanogaster. The genome of Musca is about five...
Fitness of anopheline mosquitoes expressing transgenes that inhibit Plasmodium development.
Moreira, Luciano A, Wang, Jing, Collins, Frank H, Jacobs-Lorena, Marcelo
One potential strategy for the control of malaria and other vector-borne diseases is the introduction into wild vector populations of genetic constructs that reduce vectorial capacity. An important...
A cell surface mucin specifically expressed in the midgut of the malaria mosquito Anopheles gambiae
Shen, Zhicheng, Dimopoulos, George, Kafatos, Fotis C., Jacobs-Lorena, Marcelo
An invertebrate intestinal mucin gene, AgMuc1, was isolated from the malaria vector mosquito Anopheles gambiae. The predicted 122-residue protein consists of a central core of seven repeating TTTTVAP...
Robust gut-specific gene expression in transgenic Aedes aegypti mosquitoes
Moreira, Luciano A., Edwards, Marten J., Adhami, Faisal, Jasinskiene, Nijole, James, Anthony A., Jacobs-Lorena, Marcelo
Genetic modification of the vectorial capacity of mosquito vectors of human disease requires promoters capable of driving gene expression with appropriate tissue and stage specificity. We report on...
Ghosh, Anil K., Ribolla, Paulo E. M., Jacobs-Lorena, Marcelo
Despite vast efforts and expenditures in the past few decades, malaria continues to kill millions of persons every year, and new approaches for disease control are urgently needed. To complete its...
Translational regulation of a specific gene during oogenesis and embryogenesis of Drosophila
Fruscoloni, Paolo, Al-Atia, Ghada R., Jacobs-Lorena, Marcelo
Polysomal and postpolysomal mRNAs were prepared from Drosophila egg chambers or embryos of different developmental stages. Cell-free translation of these mRNAs followed by two-dimensional gel...
Complexity of RNA in Eggs of DROSOPHILA MELANOGASTER and MUSCA DOMESTICA
Hough-Evans, Barbara R., Jacobs-Lorena, Marcelo, Cummings, Michael R., Britten, Roy J., Davidson, Eric H.
Comparative measurements are presented of the sequence complexity of the RNA stored in the eggs of two dipteran flies, Musca domestica and Drosophila melanogaster. The genome of Musca is about five...
An immune-responsive serpin, SRPN6, mediates mosquito defense against malaria parasites
Abraham, Eappen G., Pinto, Sofia B., Ghosh, Anil, Vanlandingham, Dana L., Budd, Aidan, Higgs, Stephen, ...
We have functionally analyzed the orthologous SRPN6 genes from Anopheles stephensi and Anopheles gambiae using phylogenetic, molecular, reverse genetic, and cell biological tools. The results...
Fitness of anopheline mosquitoes expressing transgenes that inhibit Plasmodium development.
Moreira, Luciano A, Wang, Jing, Collins, Frank H, Jacobs-Lorena, Marcelo
One potential strategy for the control of malaria and other vector-borne diseases is the introduction into wild vector populations of genetic constructs that reduce vectorial capacity. An important...
Marrelli, Mauro T., Li, Chaoyang, Rasgon, Jason L., Jacobs-Lorena, Marcelo
The introduction of genes that impair Plasmodium development into mosquito populations is a strategy being considered for malaria control. The effect of the transgene on mosquito fitness is a crucial...
Yoshida, Shigeto, Shimada, Yohei, Kondoh, Daisuke, Kouzuma, Yoshiaki, Ghosh, Anil K, Jacobs-Lorena, Marcelo, ...
The midgut environment of anopheline mosquitoes plays an important role in the development of the malaria parasite. Using genetic manipulation of anopheline mosquitoes to change the environment in...
Dinglasan, Rhoel R., Kalume, Dario E., Kanzok, Stefan M., Ghosh, Anil K., Muratova, Olga, Pandey, Akhilesh, ...
Malaria parasites must undergo development within mosquitoes to be transmitted to a new host. Antivector transmission-blocking vaccines inhibit parasite development by preventing ookinete interaction...
Dinglasan, Rhoel R., Alaganan, Aditi, Ghosh, Anil K., Saito, Akio, Van Kuppevelt, Toin H., Jacobs-Lorena, Marcelo
Malaria transmission entails development of the Plasmodium parasite in its insect vector, the Anopheles mosquito. Parasite invasion of the mosquito midgut is the critical first step and involves...
Distinct Roles of Plasmodium Rhomboid 1 in Parasite Development and Malaria Pathogenesis
Srinivasan, Prakash, Coppens, Isabelle, Jacobs-Lorena, Marcelo
Invasion of host cells by the malaria parasite involves recognition and interaction with cell-surface receptors. A wide variety of parasite surface proteins participate in this process, most of which...
Ghosh, Anil K., Devenport, Martin, Jethwaney, Deepa, Kalume, Dario E., Pandey, Akhilesh, Anderson, Vernon E., ...
SM1 is a twelve-amino-acid peptide that binds tightly to the Anopheles salivary gland and inhibits its invasion by Plasmodium sporozoites. By use of UV-crosslinking experiments between the peptide...
The Glutathione Biosynthetic Pathway of Plasmodium Is Essential for Mosquito Transmission
Vega-Rodríguez, Joel, Franke-Fayard, Blandine, Dinglasan, Rhoel R., Janse, Chris J., Pastrana-Mena, Rebecca, Waters, Andrew P., ...
Infection of red blood cells (RBC) subjects the malaria parasite to oxidative stress. Therefore, efficient antioxidant and redox systems are required to prevent damage by reactive oxygen species....