Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli
Di Lorenzo, Mirella, Hidalgo, Aurelio, Haas, Michael, Bornscheuer, Uwe T.
To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its...
Heterologous Production of Functional Forms of Rhizopus oryzae Lipase in Escherichia coli
Di Lorenzo, Mirella, Hidalgo, Aurelio, Haas, Michael, Bornscheuer, Uwe T.
To date, expression of the lipase from Rhizopus oryzae (ROL) in Escherichia coli always led to the formation of inclusion bodies and inactive protein. However, the production of active ROL and its...
Di Lorenzo, Mirella, Hidalgo, Aurelio, Molina, Rafael, Hermoso, Juan A., Pirozzi, Domenico, Bornscheuer, Uwe T.
A prolipase from Rhizopus oryzae (proROL) was engineered in order to increase its stability toward lipid oxidation products such as aldehydes with the aim of improving its performance in oleochemical...