Nirbhay Kumar

Details der Publikationsliste

Zeitraum

1980 - 2009

Anzahl

43

Co-Autoren

Plasmodium falciparumenolase: stage-specific expression and sub-cellular localization (2009)

Pal Bhowmick, Ipsita, Kumar, Nirbhay, Sharma, Shobhona, Coppens, Isabelle, Jarori, Gotam K

Abstract Background In an earlier study, it was observed that the vaccination with Plasmodium falciparum enolase can confer partial protection against malaria in mice. Evidence has also build up to...

Host microsatellite alleles in malaria predisposition? (2005)

Gaikwad, Sonali, Ashma, Richa, Kumar, Nirbhay, Trivedi, Rajni, Kashyap, VK

Abstract Background Malaria is a serious, sometimes fatal, disease caused by Plasmodium infection of human red blood cells. The host-parasite co-evolutionary processes are well understood by the...

Genome annotation of Anopheles gambiaeusing mass spectrometry-derived data (2005)

Kalume, Dário E, Peri, Suraj, Reddy, Raghunath, Zhong, Jun, Okulate, Mobolaji, Kumar, Nirbhay, ...

Abstract Background A large number of animal and plant genomes have been completely sequenced over the last decade and are now publicly available. Although genomes can be rapidly sequenced,...

EXACERBATION OF PLASMODIUM YOELII MALARIA IN ECHINOSTOMA CAPRONI INFECTED MICE AND ABATEMENT THROUGH ANTHELMINTIC TREATMENT (2005)

Gregory S. Noland, Thaddeus K. Graczyk, Bernard Fried, Erik J. Fitzgerald, Nirbhay Kumar

The effect of chronic intestinal trematode infection on malaria was examined in a murine model of co-infection using Echinostoma caproni and Plasmodium yoelii. BALB/c mice (n = 32) infected with a...

Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites. (2000)

Brennan, Justin D. G., Kent, Melissa, Dhar, Ravi, Fujioka, Hisashi, Kumar, Nirbhay

Anopheles gambiae is the primary vector of human malaria in sub-Saharan Africa. Invasion of Anopheles salivary glands by Plasmodium sporozoites is a necessary step in the transmission of malaria and...

Immunogenicity and in vitro protective efficacy of a recombinant multistage Plasmodium falciparum candidate vaccine. (1999)

Shi, Ya Ping, Hasnain, Seyed E., Sacci, John B., Holloway, Brian P., Fujioka, Hisashi, Kumar, Nirbhay, ...

Compared with a single-stage antigen-based vaccine, a multistage and multivalent Plasmodium falciparum vaccine would be more efficacious by inducing "multiple layers" of immunity. We have constructed...

Immunogenicity and in vitro protective efficacy of a recombinant multistage Plasmodium falciparum candidate vaccine

Shi, Ya Ping, Hasnain, Seyed E., Sacci, John B., Holloway, Brian P., Fujioka, Hisashi, Kumar, Nirbhay, ...

Compared with a single-stage antigen-based vaccine, a multistage and multivalent Plasmodium falciparum vaccine would be more efficacious by inducing “multiple layers” of immunity. We have...

Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites

Brennan, Justin D. G., Kent, Melissa, Dhar, Ravi, Fujioka, Hisashi, Kumar, Nirbhay

Anopheles gambiae is the primary vector of human malaria in sub-Saharan Africa. Invasion of Anopheles salivary glands by Plasmodium sporozoites is a necessary step in the transmission of malaria and...

Murine γδ T Lymphocytes Elicited during Plasmodium yoelii Infection Respond to Plasmodium Heat Shock Proteins

Kopacz, Jeffrey, Kumar, Nirbhay

γδ T cells accumulate during Plasmodium infections in both murine and human malarias. The biological role of these cells and the antigens that they recognize are not clearly understood, although...

Immunization of Mice with DNA-Based Pfs25 Elicits Potent Malaria Transmission-Blocking Antibodies

Lobo, Cheryl Ann, Dhar, Ravi, Kumar, Nirbhay

Immunological intervention, in addition to vector control and malaria chemotherapy, will be needed to stop the resurgence of malaria, a disease with a devastating impact on the health of 300 to 500...

Immunogenicity and Protective Efficacy of a Plasmodium yoelii Hsp60 DNA Vaccine in BALB/c Mice

Sanchez, Gloria I., Sedegah, Martha, Rogers, William O., Jones, Trevor R., Sacci, John, Witney, Adam, ...

The gene encoding the 60-kDa heat shock protein of Plasmodium yoelii (PyHsp60) was cloned into the VR1012 and VR1020 mammalian expression vectors. Groups of 10 BALB/c mice were immunized...

Mapping of Specific and Promiscuous HLA-DR-Restricted T-Cell Epitopes on the Plasmodium falciparum 27-Kilodalton Sexual Stage-Specific Antigen

Contreras, Carmen E., Ploton, Isabelle N., Siliciano, Robert F., Karp, Christopher L., Viscidi, Raphael, Kumar, Nirbhay

We have characterized HLA-DR-restricted T-cell epitopes on the 27-kDa protein (Pfg27), a sexual stage-specific antigen, of the human malaria parasite Plasmodium falciparum in subjects with a history...

Purified Malaria Pigment (Hemozoin) Enhances Dendritic Cell Maturation and Modulates the Isotype of Antibodies Induced by a DNA Vaccine

Coban, Cevayir, Ishii, Ken J., Sullivan, David J., Kumar, Nirbhay

Hemozoin (malaria pigment) has been implicated in the modulation of immune responses during malaria infection. This study was designed to evaluate the effect of purified hemozoin on the in vitro...

Induction of Plasmodium falciparum Transmission-Blocking Antibodies in Nonhuman Primates by a Combination of DNA and Protein Immunizations

Coban, Cevayir, Philipp, Mario T., Purcell, Jeanette E., Keister, David B., Okulate, Mobolaji, Martin, Dale S., ...

Malaria transmission-blocking vaccination can effectively reduce and/or eliminate transmission of parasites from the human host to the mosquito vector. The immunity achieved by inducing an antibody...

Effect of CpG Oligodeoxynucleotides on the Immunogenicity of Pfs25, a Plasmodium falciparum Transmission-Blocking Vaccine Antigen

Coban, Cevayir, Ishii, Ken J., Stowers, Anthony W., Keister, David B., Klinman, Dennis M., Kumar, Nirbhay

Antibodies directed against Pfs25, a protein present on the surface of zygotes and ookinetes of Plasmodium falciparum, completely block pathogen transmission. We evaluated the immunomodulatory effect...

Mycobacterium-Induced Potentiation of Type 1 Immune Responses and Protection against Malaria Are Host Specific

Page, Kathleen R., Jedlicka, Anne E., Fakheri, Benjamin, Noland, Gregory S., Kesavan, Anup K., Scott, Alan L., ...

Malaria and tuberculosis are endemic in many regions of the world, and coinfection with the two pathogens is common. In this study, we examined the effects of long- and short-term infection with...

Infection with Plasmodium berghei Boosts Antibody Responses Primed by a DNA Vaccine Encoding Gametocyte Antigen Pbs48/45

Haddad, Diana, Maciel, Jorge, Kumar, Nirbhay

An important consideration in the development of a malaria vaccine for individuals living in areas of endemicity is whether vaccine-elicited immune responses can be boosted by natural infection. To...

Involvement of Gonadal Steroids and Gamma Interferon in Sex Differences in Response to Blood-Stage Malaria Infection†

Cernetich, Amy, Garver, Lindsey S., Jedlicka, Anne E., Klein, Pamela W., Kumar, Nirbhay, Scott, Alan L., ...

To examine the hormonal and immunological mechanisms that mediate sex differences in susceptibility to malaria infection, intact and gonadectomized (gdx) C57BL/6 mice were inoculated with Plasmodium...

Immunogenicity and in vitro protective efficacy of a recombinant multistage Plasmodium falciparum candidate vaccine

Shi, Ya Ping, Hasnain, Seyed E., Sacci, John B., Holloway, Brian P., Fujioka, Hisashi, Kumar, Nirbhay, ...

Compared with a single-stage antigen-based vaccine, a multistage and multivalent Plasmodium falciparum vaccine would be more efficacious by inducing “multiple layers” of immunity. We have...

Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites

Brennan, Justin D. G., Kent, Melissa, Dhar, Ravi, Fujioka, Hisashi, Kumar, Nirbhay

Anopheles gambiae is the primary vector of human malaria in sub-Saharan Africa. Invasion of Anopheles salivary glands by Plasmodium sporozoites is a necessary step in the transmission of malaria and...

Murine γδ T Lymphocytes Elicited during Plasmodium yoelii Infection Respond to Plasmodium Heat Shock Proteins

Kopacz, Jeffrey, Kumar, Nirbhay

γδ T cells accumulate during Plasmodium infections in both murine and human malarias. The biological role of these cells and the antigens that they recognize are not clearly understood, although...

Immunization of Mice with DNA-Based Pfs25 Elicits Potent Malaria Transmission-Blocking Antibodies

Lobo, Cheryl Ann, Dhar, Ravi, Kumar, Nirbhay

Immunological intervention, in addition to vector control and malaria chemotherapy, will be needed to stop the resurgence of malaria, a disease with a devastating impact on the health of 300 to 500...

Immunogenicity and Protective Efficacy of a Plasmodium yoelii Hsp60 DNA Vaccine in BALB/c Mice

Sanchez, Gloria I., Sedegah, Martha, Rogers, William O., Jones, Trevor R., Sacci, John, Witney, Adam, ...

The gene encoding the 60-kDa heat shock protein of Plasmodium yoelii (PyHsp60) was cloned into the VR1012 and VR1020 mammalian expression vectors. Groups of 10 BALB/c mice were immunized...

Mapping of Specific and Promiscuous HLA-DR-Restricted T-Cell Epitopes on the Plasmodium falciparum 27-Kilodalton Sexual Stage-Specific Antigen

Contreras, Carmen E., Ploton, Isabelle N., Siliciano, Robert F., Karp, Christopher L., Viscidi, Raphael, Kumar, Nirbhay

We have characterized HLA-DR-restricted T-cell epitopes on the 27-kDa protein (Pfg27), a sexual stage-specific antigen, of the human malaria parasite Plasmodium falciparum in subjects with a history...

Purified Malaria Pigment (Hemozoin) Enhances Dendritic Cell Maturation and Modulates the Isotype of Antibodies Induced by a DNA Vaccine

Coban, Cevayir, Ishii, Ken J., Sullivan, David J., Kumar, Nirbhay

Hemozoin (malaria pigment) has been implicated in the modulation of immune responses during malaria infection. This study was designed to evaluate the effect of purified hemozoin on the in vitro...

Induction of Plasmodium falciparum Transmission-Blocking Antibodies in Nonhuman Primates by a Combination of DNA and Protein Immunizations

Coban, Cevayir, Philipp, Mario T., Purcell, Jeanette E., Keister, David B., Okulate, Mobolaji, Martin, Dale S., ...

Malaria transmission-blocking vaccination can effectively reduce and/or eliminate transmission of parasites from the human host to the mosquito vector. The immunity achieved by inducing an antibody...

Effect of CpG Oligodeoxynucleotides on the Immunogenicity of Pfs25, a Plasmodium falciparum Transmission-Blocking Vaccine Antigen

Coban, Cevayir, Ishii, Ken J., Stowers, Anthony W., Keister, David B., Klinman, Dennis M., Kumar, Nirbhay

Antibodies directed against Pfs25, a protein present on the surface of zygotes and ookinetes of Plasmodium falciparum, completely block pathogen transmission. We evaluated the immunomodulatory effect...

Mycobacterium-Induced Potentiation of Type 1 Immune Responses and Protection against Malaria Are Host Specific

Page, Kathleen R., Jedlicka, Anne E., Fakheri, Benjamin, Noland, Gregory S., Kesavan, Anup K., Scott, Alan L., ...

Malaria and tuberculosis are endemic in many regions of the world, and coinfection with the two pathogens is common. In this study, we examined the effects of long- and short-term infection with...

Infection with Plasmodium berghei Boosts Antibody Responses Primed by a DNA Vaccine Encoding Gametocyte Antigen Pbs48/45

Haddad, Diana, Maciel, Jorge, Kumar, Nirbhay

An important consideration in the development of a malaria vaccine for individuals living in areas of endemicity is whether vaccine-elicited immune responses can be boosted by natural infection. To...

Involvement of Gonadal Steroids and Gamma Interferon in Sex Differences in Response to Blood-Stage Malaria Infection†

Cernetich, Amy, Garver, Lindsey S., Jedlicka, Anne E., Klein, Pamela W., Kumar, Nirbhay, Scott, Alan L., ...

To examine the hormonal and immunological mechanisms that mediate sex differences in susceptibility to malaria infection, intact and gonadectomized (gdx) C57BL/6 mice were inoculated with Plasmodium...

Aquaglyceroporin PbAQP during intraerythrocytic development of the malaria parasite Plasmodium berghei

Promeneur, Dominique, Liu, Yangjian, Maciel, Jorge, Agre, Peter, King, Landon S., Kumar, Nirbhay

The malaria parasite can use host plasma glycerol for lipid biosynthesis and membrane biogenesis during the asexual intraerythrocytic development. The molecular basis for glycerol uptake into the...

Aquaporin 9 is the major pathway for glycerol uptake by mouse erythrocytes, with implications for malarial virulence

Liu, Yangjian, Promeneur, Dominique, Rojek, Aleksandra, Kumar, Nirbhay, Frøkiær, Jørgen, Nielsen, Søren, ...

Human and rodent erythrocytes are known to be highly permeable to glycerol. Aquaglyceroporin aquaporin (AQP)3 is the major glycerol channel in human and rat erythrocytes. However, AQP3 expression has...

Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoin

Coban, Cevayir, Ishii, Ken J., Kawai, Taro, Hemmi, Hiroaki, Sato, Shintaro, Uematsu, Satoshi, ...

Malaria parasites within red blood cells digest host hemoglobin into a hydrophobic heme polymer, known as hemozoin (HZ), which is subsequently released into the blood stream and then captured by and...

Murine Model for Assessment of Plasmodium falciparum Transmission-Blocking Vaccine Using Transgenic Plasmodium berghei Parasites Expressing the Target Antigen Pfs25▿

Mlambo, Godfree, Maciel, Jorge, Kumar, Nirbhay

Currently, there is no animal model for Plasmodium falciparum challenge to evaluate malaria transmission-blocking vaccines based on the well-established Pfs25 target antigen. The biological activity...

Critical role of a K+ channel in Plasmodium berghei transmission revealed by targeted gene disruption

Ellekvist, Peter, Maciel, Jorge, Mlambo, Godfree, Ricke, Christina H., Colding, Hanne, Klaerke, Dan A., ...

Regulated K+ transport across the plasma membrane is of vital importance for the survival of most cells. Two K+ channels have been identified in the Plasmodium falciparum genome; however, their...

Malaria Parasite Invasion of the Mosquito Salivary Gland Requires Interaction between the Plasmodium TRAP and the Anopheles Saglin Proteins

Ghosh, Anil K., Devenport, Martin, Jethwaney, Deepa, Kalume, Dario E., Pandey, Akhilesh, Anderson, Vernon E., ...

SM1 is a twelve-amino-acid peptide that binds tightly to the Anopheles salivary gland and inhibits its invasion by Plasmodium sporozoites. By use of UV-crosslinking experiments between the peptide...

A Potent Malaria Transmission Blocking Vaccine Based on Codon Harmonized Full Length Pfs48/45 Expressed in Escherichia coli

Chowdhury, Debabani Roy, Angov, Evelina, Kariuki, Thomas, Kumar, Nirbhay

Malaria caused by Plasmodium falciparum is responsible for nearly 1 million deaths annually. Although much progress has been made in the recent past, the development of a safe, effective and...