Real-time fluorescence detection of exoribonucleases (2009)
Sinturel, Flore, Pellegrini, Olivier, Xiang, Song, Tong, Liang, Condon, Ciarán, Bénard, Lionel
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic...
Arluison, Véronique, Hohng, Sungchul, Roy, Rahul, Pellegrini, Olivier, Régnier, Philippe, Ha, Taekjip
Hfq protein is vital for the function of many non-coding small (s)RNAs in bacteria but the mechanism by which Hfq facilitates the function of sRNA is still debated. We developed a fluorescence...
Arluison, Véronique, Hohng, Sungchul, Roy, Rahul, Pellegrini, Olivier, Régnier, Philippe, Ha, Taekjip
Hfq protein is vital for the function of many non-coding small (s)RNAs in bacteria but the mechanism by which Hfq facilitates the function of sRNA is still debated. We developed a fluorescence...
Hfq variant with altered RNA binding functions (2006)
Ziolkowska, Katarzyna, Derreumaux, Philippe, Folichon, Marc, Pellegrini, Olivier, Régnier, Philippe, Boni, Irina V., ...
The interaction between Hfq and RNA is central to multiple regulatory processes. Using site-directed mutagenesis, we have found a missense mutation in Hfq (V43R) which strongly affects2 the RNA...
Even, Sergine, Pellegrini, Olivier, Zig, Lena, Labas, Valerie, Vinh, Joelle, Bréchemmier-Baey, Dominique, ...
Many prokaryotic organisms lack an equivalent of RNase E, which plays a key role in mRNA degradation in Escherichia coli. In this paper, we report the purification and identification by mass...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis
Pellegrini, Olivier, Nezzar, Jamel, Marchfelder, Anita, Putzer, Harald, Condon, Ciarán
In contrast to Escherichia coli, where the 3′ ends of tRNAs are primarily generated by exoribonucleases, maturation of the 3′ end of tRNAs is catalysed by an endoribonuclease, known as RNase Z...
The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation
Folichon, Marc, Arluison, Véronique, Pellegrini, Olivier, Huntzinger, Eric, Régnier, Philippe, Hajnsdorf, Eliane
The Hfq protein, which shares sequence and structural homology with the Sm and Lsm proteins, binds to various RNAs, primarily recognizing AU-rich single-stranded regions. In this paper, we study the...
Even, Sergine, Pellegrini, Olivier, Zig, Lena, Labas, Valerie, Vinh, Joelle, Bréchemmier-Baey, Dominique, ...
Many prokaryotic organisms lack an equivalent of RNase E, which plays a key role in mRNA degradation in Escherichia coli. In this paper, we report the purification and identification by mass...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Hfq variant with altered RNA binding functions
Ziolkowska, Katarzyna, Derreumaux, Philippe, Folichon, Marc, Pellegrini, Olivier, Régnier, Philippe, Boni, Irina V., ...
The interaction between Hfq and RNA is central to multiple regulatory processes. Using site-directed mutagenesis, we have found a missense mutation in Hfq (V43R) which strongly affects2 the RNA...
Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis
Pellegrini, Olivier, Nezzar, Jamel, Marchfelder, Anita, Putzer, Harald, Condon, Ciarán
In contrast to Escherichia coli, where the 3′ ends of tRNAs are primarily generated by exoribonucleases, maturation of the 3′ end of tRNAs is catalysed by an endoribonuclease, known as RNase Z...
The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation
Folichon, Marc, Arluison, Véronique, Pellegrini, Olivier, Huntzinger, Eric, Régnier, Philippe, Hajnsdorf, Eliane
The Hfq protein, which shares sequence and structural homology with the Sm and Lsm proteins, binds to various RNAs, primarily recognizing AU-rich single-stranded regions. In this paper, we study the...
Even, Sergine, Pellegrini, Olivier, Zig, Lena, Labas, Valerie, Vinh, Joelle, Bréchemmier-Baey, Dominique, ...
Many prokaryotic organisms lack an equivalent of RNase E, which plays a key role in mRNA degradation in Escherichia coli. In this paper, we report the purification and identification by mass...
Wen, Tingyi, Oussenko, Irina A., Pellegrini, Olivier, Bechhofer, David H., Condon, Ciarán
In contrast to Escherichia coli, where all tRNAs have the CCA motif encoded by their genes, two classes of tRNA precursors exist in the Gram-positive bacterium Bacillus subtilis. Previous evidence...
Hfq variant with altered RNA binding functions
Ziolkowska, Katarzyna, Derreumaux, Philippe, Folichon, Marc, Pellegrini, Olivier, Régnier, Philippe, Boni, Irina V., ...
The interaction between Hfq and RNA is central to multiple regulatory processes. Using site-directed mutagenesis, we have found a missense mutation in Hfq (V43R) which strongly affects2 the RNA...
Arluison, Véronique, Hohng, Sungchul, Roy, Rahul, Pellegrini, Olivier, Régnier, Philippe, Ha, Taekjip
Hfq protein is vital for the function of many non-coding small (s)RNAs in bacteria but the mechanism by which Hfq facilitates the function of sRNA is still debated. We developed a fluorescence...