P. Büscher

Details der Publikationsliste

Zeitraum

2000 - 2009

Anzahl

114

Co-Autoren

Identification of Old World Leishmania spp. by specific polymerase chain reaction amplification of cysteine proteinase B genes and rapid dipstick detection (2009)

Laurent, T., Hide, M., Mertens, P., Quispe Tintaya, W., Deborggraeve, S., ...

We used the cysteine proteinase B (cpb) gene family of the trypanosomatid genus Leishmania as a target to develop rapid, specific, and easy-to-use polymerase chain reaction (PCR) tests to...

Comparison of operational criteria for treatment outcome in gambiense human African trypanosomiasis (2009)

Ngoyi, D. M., Lejon, V., N'Siesi, F. X., Boelaert, M., Büscher, P.

OBJECTIVE: To develop a simple and standard operational decision tool for the diagnosis of relapse after treatment for human African trypanosomiasis (HAT), by evaluating the performance of several...

T. cruzi OligoC-TesT: a simplified and standardized polymerase chain reaction format for diagnosis of Chagas disease (2009)

Deborggraeve, S., Coronado, X., Solari, A., Zulantay, I., Apt, W., Mertens, P., ...

BACKGROUND: PCR has evolved into one of the most promising tools for T. cruzi detection in the diagnosis and control of Chagas disease. However, general use of the technique is hampered by its...

Bioluminescent imaging of Trypanosoma brucei shows preferential testis dissemination which may hamper drug efficacy in sleeping sickness (2009)

Claes, F., Vodnala, S. K., Van Reet, N., Boucher, N., Lunden-Miguel, H., Baltz, T., ...

Monitoring Trypanosoma spread using real-time imaging in vivo provides a fast method to evaluate parasite distribution especially in immunoprivileged locations. Here, we generated monomorphic and...

Towards a new reference test for surra in camels (2009)

Tran, T., Claes, F., Verloo, D., De Greve, H., Büscher, P.

Current serological diagnosis of Trypanosoma evansi infection in camels is based on the native variable antigen type RoTat 1.2. The goal of this study was to develop a novel serological diagnostic...

Leishmania OligoC-TesT as a simple, rapid, and standardized tool for molecular diagnosis of cutaneous leishmaniasis in Peru (2009)

Espinosa, D., Boggild, A. K., Deborggraeve, S., Laurent, T., Valencia, C., Pacheco, R., ...

Molecular methods such as PCR have become attractive tools for diagnosis of cutaneous leishmaniasis (CL), both for their high sensitivity and for their specificity. However, their practical use in...

Novel markers for treatment outcome in late-stage Trypanosoma brucei gambiense trypanosomiasis (2008)

Lejon, V., Roger, I., Ngoyi, D. M., Robays, J., N'Siesi, X., Bisser, S., ...

BACKGROUND: To date, no biological marker for treatment outcome in human African trypanosomiasis (HAT) has been described. The accuracy of biological markers for prediction of treatment outcome of...

Heterologous expression, purification and characterisation of the extracellular domain of trypanosome invariant surface glycoprotein ISG75 (2008)

Tran, T., Büscher, P., Vandenbussche, G., Wyns, L., Messens, J., De Greve, H.

The invariant surface glycoprotein ISG75 is a transmembrane glycoprotein occurring on the surface of the bloodstream-form Trypanozoon. This study describes the expression and purification of the...

Molecular analysis of archived blood slides reveals an atypical human Trypanosoma infection (2008)

Deborggraeve, S., Koffi, M., Jamonneau, V., Bonsu, F. A., Queyson, R., Simarro, P. P., ...

In 2003, a 10-month-old Ghanaian boy recovered from a Trypanosoma brucei infection, although the patient was not treated with antitrypanosomal drugs. Only T. brucei gambiense and T. brucei...

A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis (2008)

Deborggraeve, S., Laurent, T., Espinosa, D., Mbuchi, M., Wasunna, M., ...

Background. Definite diagnosis of Leishmania infections is based on demonstration of the parasite by microscopic analysis of tissue biopsy specimens or aspirate samples. However, microscopy generally...

Presence of Trypanosoma theileri in Spanish cattle (2008)

Villa, A., Gutierrez, C., Gracia, E., Moreno, B., Chacón, G., Sanz, P. V., ...

Trypanosoma theileri (Laveran, 1902) has been diagnosed in many countries and is commonly considered as a nonpathogenic hemoparasite, although some authors have described clinical signs in cattle...

Refinement recommendations for intravenous injection and body heat conservation in Mongolian gerbils used in SPECT studies (2008)

Moons, C. P. H., Peremans, K., Büscher, P., Vermeire, S., Vandermeulen, E., Audenaert, K., ...

This paper describes two aspects of Single Photon Emission Computed Tomography (SPECT) which have been refined for use in small animals, as demonstrated by a study on Mongolian gerbils: intravenous...

Validation of a PCR-Oligochromatography test for Trypanozoon parasites in a multicenter collaborative trial (2007)

Claes, Filip; U0032450, Deborggraeve, Stijn; U0046640, Verloo, D, Mertens, P, Crowther, J R, Leclipteux, T, ...

A PCR-Oligochromatography test for diagnosis of human and animal trypanosomosis was evaluated through a multicenter ringtrial with 6 laboratories testing a set of 21 blinded samples, resulting in...

Equivalence trial of melarsoprol and nifurtimox monotherapy and combination therapy for the treatment of second-stage Trypanosoma brucei gambiense sleeping sickness (2007)

Bisser, S., N'Siesi, F. X., Lejon, V., Preux, P. M., Van Nieuwenhove, S., ...

BACKGROUND: Treatment of second-stage sleeping sickness relies mainly on melarsoprol. Nifurtimox has been successfully used to cure melarsoprol-refractory sleeping sickness caused by Trypanosoma...

Evaluation of serodiagnostic tests for T. b. gambiense human African trypanosomiasis in southern Sudan (2007)

Elrayah, I. E., Rhaman, M. A., Karamalla, L. T., Khalil, K. M., Büscher, P.

A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei...

Establishment of a panel of reference Trypanosoma evansi and Trypanosoma equiperdum strains for drug screening (2007)

Gillingwater, K., Büscher, P., Brun, R.

The animal pathogenic protozoan, Trypanosoma evansi, leads to a wasting disease in equines, cattle and camels, commonly known as Surra. It is extensively distributed geographically with a wide range...

Treatment failure related to intrathecal immunoglobulin M (IgM) synthesis, cerebrospinal fluid IgM, and interleukin-10 in patients with hemolymphatic-stage sleeping sickness (2007)

Lejon, V., Robays, J., N'Siesi, F. X., Mumba, D., Hoogstoel, A., Bisser, S., ...

Human African trypanosomiasis treatment is stage dependent, but the tests used for staging are controversial. Central nervous system involvement and its relationship with suramin treatment failure...

Identification of a tryptophan-like epitope borne by the variable surface glycoprotein (VSG) of African trypanosomes (2007)

Semballa, S., Okomo-Assoumou, M. C., Holzmuller, P., Büscher, P., Magez, S., Lemesre, J. L., ...

Antibodies (Ab) directed against a tryptophan-like epitope (WE) were previously detected in patients with human African trypanosomiasis (HAT). We investigated whether or not these Ab resulted from...

Cost-effectiveness of algorithms for confirmation test of human African trypanosomiasis (2007)

Lutumba, P., Meheus, F., Robays, J., Miaka, C., Kande, V., Büscher, P., ...

The control of Trypanosoma brucei gambiense human African trypanosomiasis (HAT) is compromised by low sensitivity of the routinely used parasitologic confirmation tests. More sensitive alternatives,...

Validation of a PCR-oligochromatography test for detection of Trypanozoon parasites in a multicenter collaborative trial (2007)

Claes, F., Deborggraeve, S., Verloo, D., Mertens, P., Crowther, J. R., Leclipteux, T., ...

A PCR-oligochromatography test for diagnosis of human and animal trypanosomiasis was evaluated through a multicenter ring trial with six laboratories testing a set of 21 blinded samples, resulting in...

Molecular dipstick test for diagnosis of sleeping sickness (2006)

Laurent, T, Mertens, P, Leclipteux, T, Dujardin, JC, ...

Human African trypanosomiasis (HAT) or sleeping sickness is a neglected disease that affects poor rural populations across sub-Saharan Africa. Confirmation of diagnosis is based on detection of...

Molecular dipstick test for diagnosis of sleeping sickness (2006)

Deborggraeve, S., Claes, F., Laurent, T., Mertens, P., Leclipteux, T., Dujardin, J. C., ...

Human African trypanosomiasis (HAT) or sleeping sickness is a neglected disease that affects poor rural populations across sub-Saharan Africa. Confirmation of diagnosis is based on detection of...

Trypanosomosis in goats; current status (2006)

Gutierrez, C., Corbera, J. A., Morales, M., Büscher, P.

Trypanosomosis is a major constraint on ruminant livestock production in Africa, Asia, and South America. The principal host species affected varies geographically, but buffalo, cattle, camels, and...

Pathology of Tnf-deficient mice infected with Plasmodium chabaudi adami 408XZ (2006)

Hernandez-Valladares, M., Naessens, J., Musoke, A. J., Sekikawa, K., Rihet, P., Ole-MoiYoi, O. K., ...

Tumor necrosis factor alpha (Tnf) plays a pleiotropic role in murine malaria. Some investigations have correlated Tnf with hypothermia, hyperlactatemia, hypoglycemia, and a suppression of the...

Aparasitemic serological suspects in Trypanosoma brucei gambiense human African trypanosomiasis: a potential human reservoir of parasites? (2006)

Koffi, M., Solano, P., Denizot, M., Courtin, D., Garcia, A., Lejon, V., ...

The serological and parasitological tests used for Trypanosoma brucei gambiense human African trypanosomiasis (HAT) diagnosis have low specificity and sensitivity, respectively, and in the field,...

The invariant surface glycoprotein ISG75 gene family consists of two main groups in the Trypanozoon subgenus (2006)

Tran, T., Claes, F., Dujardin, J. C., Büscher, P.

In Trypanosoma brucei brucei, an invariant surface glycoprotein of molecular weight 75 kDa (ISG75) is uniformly distributed over the surface of a trypanosome and is specific for bloodstream-form...

Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels (2005)

Verloo, D, Maina, M, Urakawa, T, Majiwa, P A O, ...

The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni...

Options for field diagnosis of human African trypanosomiasis (2005)

Chappuis, F., Loutan, L., Simarro, P., Lejon, V., Büscher, P.

Human African trypanosomiasis (HAT) due to Trypanosoma brucei gambiense or T. b. rhodesiense remains highly prevalent in several rural areas of sub-Saharan Africa and is lethal if left untreated....

Trypanosoma equiperdum: master of disguise or historical mistake? (2005)

Claes, F., Büscher, P., Touratier, L., Goddeeris, B. M.

After 100 years of research, only a small number of laboratory strains of Trypanosoma equiperdum exists, and the history of most of the strains is unknown. No definitive diagnosis of dourine can be...

Comparison of serological tests for equine trypanosomosis in naturally infected horses from Kazakhstan (2005)

Claes, F., Ilgekbayeva, G. D., Verloo, D., Saidouldin, T. S., Geerts, S., Büscher, P., ...

In this study, we compared the complement fixation test (CFT), the horse complement fixation test (HCFT) and a card agglutination test for trypanosomosis (CATT/T. evansi) for the diagnosis of equine...

Murine malaria parasite sequestration: CD36 is the major receptor, but cerebral pathology is unlinked to sequestration (2005)

Franke-Fayard, B., Janse, C. J., Cunha-Rodrigues, M., Ramesar, J., Büscher, P., Que, I., ...

Sequestration of malaria-parasite-infected erythrocytes in the microvasculature of organs is thought to be a significant cause of pathology. Cerebral malaria (CM) is a major complication of...

Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels (2005)

Lejon, V., Claes, F., Verloo, D., Maina, M., Urakawa, T., Majiwa, P. A. O., ...

The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni...

Variable surface glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections (2004)

Claes, F., Radwanska, M., Urakawa, T., Majiwa, P. A. O., Goddeeris, B., Büscher, P.

BACKGROUND: Based on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region...

Use of the miniature anion exchange centrifugation technique to isolate Trypanosoma evansi from goats (2004)

Gutierrez, C., Corbera, J. A., Doreste, F., Büscher, P.

DEAE (anion exchanger diethylaminoethyl)-cellulose and mini Anion Exchange Centrifugation Technique (mAECT) allow salivarian trypanosomes to be separated from the blood of affected animals. The...

Performance of serological tests for Trypanosoma evansi in experimentally inoculated goats (2004)

Gutierrez, C., Corbera, J. A., Morales, M., Büscher, P.

Natural Trypanosoma evansi infection in the Canary Islands has only been diagnosed in the camel population, but dissemination of the disease in other hosts has not been excluded. The objective of...

Evaluation of the card agglutination test (CATT/T. evansi) for detection of Trypanosoma evansi infection in water buffaloes (Bubalis bubalis) in Egypt (2004)

Hilali, M., Abdel-Gawad, A., Nassar, A., Abdel-Wahab, A., Magnus, E., Büscher, P.

A survey to investigate resistance to drugs used in the treatment of bovine trypanosomosis was conducted in the eastern province of Zambia between 1996 and 1998. A cross-sectional study was conducted...

Trypanosoma vivax: a simplified protocol for in vivo growth, isolation and cryopreservation (2004)

Ndao, M., Magnus, E., Büscher, P., Geerts, S.

A rodent adapted clone of Trypanosoma vivax was used to infect cyclophosphamide treated mice and rats. Fresh blood containing trypanosomes, was centrifuged in a density gradient of three Percoll...

The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum (2003)

Verloo, D, De Waal, D T, Majiwa, P A O, Baltz, T, ...

In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma...

How does Trypanosoma equiperdum fit into the Trypanozoon group? A cluster analysis by RAPD and multiplex-endonuclease genotyping approach (2003)

Agbo, E C, Radwanska, M, Baltz, T, De Waal, D T, ...

The pathogenic trypanosomes Trypanosoma equiperdum, T. evansi as well as T. brucei are morphologically identical. In horses, these parasites are considered to cause respectively dourine, surra and...

Population genetic structure and cladistic analysis of Trypanosoma brucei isolates (2003)

Agbo, E. C., Clausen, P. H., Büscher, P., Majiwa, P. A. O., Claassen, E.

Using a novel multilocus DNA marker analysis method, we studied the population genetic structure of Trypansoma brucei stocks and derived clones isolated from animal and rhodesiense sleeping sickness...

Multiplex-endonuclease genotyping approach (MEGA): a tool for the fine-scale detection of unlinked polymorphic DNA markers (2003)

Agbo, E. C., Duim, B., Majiwa, P. A., Büscher, P., Claassen, E., Te Pas, M. F.

Restriction enzyme-detectable polymorphisms have been used for assessing genetic differences and generating informative genetic markers. The most detailed fingerprinting analyses have been obtained...

How does Trypanosoma equiperdum fit into the Trypanozoon group? A cluster analysis by RAPD and multiplex-endonuclease genotyping approach (2003)

Claes, F., Agbo, E. C., Radwanska, M., Baltz, T., De Waal, D. T., ...

The pathogenic trypanosomes Trypanosoma equiperdum, T. evansi as well as T. brucei are morphologically identical. In horses, these parasites are considered to cause respectively dourine, surra and...

The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum (2003)

Claes, F., Verloo, D., De Waal, D. T., Majiwa, P. A. O., Baltz, T., Goddeeris, B. M., ...

In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma...

The challenge of Trypanosoma brucei gambiense sleeping sickness diagnosis outside Africa (2003)

Lejon, V., Boelaert, M., Jannin, J., Moore, A., Büscher, P.

Sleeping sickness is a lethal African disease caused by parasites of the Trypanosoma brucei subspecies, which is transmitted by tsetse flies. Occasionally, patients are reported outside Africa....

Towards saliva-based screening for sleeping sickness? (2003)

Lejon, V., Kwete, J., Büscher, P.

The definitive version is available at www3.interscience.wiley.com

Neuro-inflammatory risk factors for treatment failure in "early second stage" sleeping sickness patients treated with Pentamidine (2003)

Lejon, V., Legros, D., Savignoni, A., Gastellu Etchegorry, M., Mbulamberi, D., Büscher, P.

In a clinical trial on efficacy of Pentamidine in second stage Trypanosoma brucei gambiense patients with /=4 and LATEX/T.b. gambiense positive CSF, were associated with treatment failure.Detection...

Performance of enzyme-linked immunosorbent assays for detection of antibodies against T. congolense and T. vivax in goats (2003)

Lejon, V., Rebeski, D. E., Ndao, M., Baelmans, R., Winger, E. M., Faye, D., ...

Indirect ELISAs using denatured antigen preparations of Trypanosoma (T.) congolense (TcAGd) and T. vivax (TvAGd) for detection of anti-trypanosome antibodies in bovine serum (I-TAB ELISAs), were...

Intrathecal immune response pattern for improved diagnosis of central nervous system involvement in trypanosomiasis (2003)

Lejon, V., Reiber, H., Legros, D., Djé, N., Magnus, E., Wouters, I., ...

Diagnosis of central nervous system (CNS) involvement in human African trypanosomiasis is crucial in determination of therapy. Cerebrospinal fluid (CSF) and serum immunoglobulin concentrations,...

Human African trypanosomiasis: quantitative and qualitative assessment of intrathecal immune response (2003)

Lejon, V., Sindic, C. J. M., Van Antwerpen, M. P., Doua, F., Solano, P., Jamonneau, V., ...

Quantitative and qualitative techniques for assessment of the intrathecal humoral immune response in human African trypanosomiasis were compared, and their diagnostic potential for detection of the...

Evaluation of LATEX/T.b.gambiense for mass screening of Trypanosoma brucei gambiense sleeping sickness in Central Africa (2003)

Penchenier, L., Grébaut, P., Njokou, F., Eboo Eyenga, V., Büscher, P.

We compared the Card Agglutination Test for Trypanosomiasis (CATT), which consists of lyophilized bloodstream form trypomastigotes of Trypanosoma brucei gambiense (T.b.g.) variable antigen type LiTat...

Blood-cerebrospinal fluid barrier and intrathecal immunoglobulins compared to field diagnosis of central nervous system involvement in sleeping sickness (2002)

Bisser, S., Lejon, V., Preux, P. M., Bouteille, B., Stanghellini, A., Jauberteau, M. O., ...

Diagnosis of central nervous system (CNS) involvement in sleeping sickness is crucial in order to give an appropriate treatment regimen. Neurological symptoms occur late, therefore field diagnosis is...

Expression of RoTat 1.2 cross-reactive variable antigen type in Trypanosoma evansi and T. equiperdum (2002)

Claes, F., Verloo, D., De Waal, D. T., Urakawa, T., Majiwa, P., Goddeeris, B. M., ...

The variable antigen type (VAT) RoTat 1.2 has been cloned from a T. evansi strain, isolated in 1982 from a water buffalo in Indonesia. All T. evansi isolates hitherto tested express this VAT. In a...

Evaluation of whole fresh blood and dried blood on filter paper discs in serological tests for Trypanosoma evansi in experimentally infected water buffaloes (2002)

Holland, W. G., Thanh, N. G., My, L. N., Magnus, E., Verloo, D., Büscher, P., ...

In this study we investigated if whole blood could substitute for serum in the direct card agglutination test (CATT/Trypansosoma evansi) and the indirect card agglutination test (LATEX/T. evansi) for...

Treatment of human African trypanosomiasis; present situation and needs for research and development (2002)

Legros, D., Ollivier, G., Gastellu Etchegorry, M., Paquet, C., Burri, C., Jannin, J., ...

Human African trypanosomiasis re-emerged in the 1980s. However, little progress has been made in the treatment of this disease over the past decades. The first-line treatment for second-stage cases...

Le diagnostic du stade dans la maladie du sommeil: vers une nouvelle approche (2002)

Lejon, V., Büscher, P.

Diagnosis of the neurological disease stage in Trypanosoma brucei (T.b.) gambiense infection is essential to select an optimal chemotherapy. The actual parameters for stage determination, the...

Interleukin (IL)-6, IL-8 and IL-10 in serum and CSF of Trypanosoma brucei gambiense sleeping sickness patients before and after treatment (2002)

Lejon, V., Lardon, J., Kenis, G., Pinoges, L., Legros, D., Bisser, S., ...

Serum and cerebrospinal fluid (CSF) concentrations of interleukin (IL)-6, IL-8, IL-10, tumour necrosis factor-alpha and interferon-gamma were determined in 46 Trypanosoma brucei gambiense sleeping...

Evaluation of an EDTA version of CATT/Trypanosoma brucei gambiense for serological screening of human blood samples (2002)

Magnus, E., Lejon, V., Bayon, D., Buyse, D., Simarro, P., Verloo, D., ...

CATT/Trypanosoma brucei gambiense, a direct card agglutination test designed for field surveys on human African trypanosomosis, is currently used with freshly collected heparinized blood samples....

The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense (2002)

Radwanska, M., Chamekh, M., Vanhamme, L., Claes, F., Magez, S., Magnus, E., ...

In the search for new diagnostic methods that would distinguish Trypanosoma brucei rhodesiense from T. b. brucei and T. b. gambiense, we have developed two polymerase chain reaction (PCR) primer...

Novel primer sequences for polymerase chain reaction -based detection of Trypanosoma brucei gambiense (2002)

Radwanska, M., Claes, F., Magez, S., Magnus, E., Perez-Morga, D., Pays, E., ...

Progress in diagnosis, treatment, and epidemiology of human African trypanosomiasis (sleeping sickness) depends on the existence of specific and sensitive diagnostic tools. Inherent shortcomings of...

Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes (2002)

Radwanska, M., Magez, S., Perry-O'Keefe, H., Stender, H., Coull, J., Sternberg, J. M., ...

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid...

Comparison of different DNA preparation protocols for PCR diagnosis of human African trypanosomiasis in Côte d'Ivoire (2002)

Solano, P., Jamonneau, V., N'Guessan, P., N'Dri, L., Dje, N. N., Miezan, T. W., ...

During a medical survey the sleeping sickness focus in Bonon, Ivory Coast, PCR with Trypanosoma brucei specific primers (TBR 1-2 from Parasitology 99 (1989) 57) was tested on DNA derived from blood...

Evaluation of the micro-CATT, CATT/Trypanosoma brucei gambiense, and LATEX/T.b. gambiense methods for serodiagnosis and surveillance of human African trypanosomiasis in West and Central Africa (2002)

Truc, P., Lejon, V., Magnus, E., Jamonneau, V., Nangouma, A., Verloo, D., ...

OBJECTIVE: To evaluate the performance of serological tests using dried blood on filter-papers (micro-card agglutination test for trypanosomiasis (micro-CATT)) performed under field and laboratory...

A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes (2001)

My, L N, Thanh, N G, Tam, P T, Verloo, D, ...

In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a...

A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes (2001)

Holland, W. G., Claes, F., My, L. N., Thanh, N. G., Tam, P. T., Verloo, D., ...

In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a...

Stage determination and follow-up in sleeping sickness (2001)

Lejon, V., Büscher, P.

In order to select a correct treatment after primary diagnosis of trypanosomiasis infection, accurate assessment of the disease stage, haemo-lymphatic or meningo-encephalitic, is essential. This is...

Biological data and clinical symptoms as predictors of astrogliosis and neurodegeneration in patients with second-stage Trypanosoma brucei gambiense sleeping sickness (2001)

Lejon, V., Legros, D., Rosengren, L., Gastellu Etchegorry, M., Büscher, P.

Concentrations of glial fibrillary acidic protein (GFAp) and light subunit neurofilament protein (NFL) in cerebrospinal fluid (CSF) were measured in patients with second-stage Trypanosoma brucei...

Le diagnostic de la trypanosomiase humaine africaine en 2001 (2001)

Louis, F. J., Büscher, P., Lejon, V.

Human African trypanosomiasis is characterized by a non-specific clinical presentation with no consistent, pathognomonic manifestations. However definite diagnosis is necessary to avoid unnecessary...

Charting methods to monitor the operational performance of ELISA method for the detection of antibodies against trypanosomes (2001)

Rebeski, D. E., Winger, E. M., Ouma, J. O., Kong Pages, S., Büscher, P., Sanogo, Y., ...

Four indirect enzyme-linked immunosorbent assays (ELISAs) for the detection of antibody against trypanosomes using antigen-precoated plates (Trypanosoma congolense and T. vivax) were used in 15...

Trypanosoma evansi: cloning and expression in Spodoptera fugiperda insect cells of the diagnostic antigen RoTat1.2 (2001)

Urakawa, T., Verloo, D., Moens, L., Büscher, P., Majiwa, P. A. O.

A complementary DNA encoding the variant surface glycoprotein (VSG) of Trypanosoma evansi Rode Trypanozoon antigenic type (RoTat)1.2, currently used for experimental serological diagnosis of T....

General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin (2001)

Verloo, D., Magnus, E., Büscher, P.

The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on...

Cross-reactivity of anti-galactocerebroside autoantibodies with a Trypanosoma brucei proteolipidic epitope (2000)

Girard, M., Bisser, S., Büscher, P., Dumas, M., Preud'homme, J. L., Jauberteau, M. O.

Pathogenic mechanisms of the demyelinating encephalopathy featuring the nervous phase of human African trypanosomiasis (HAT) are largely unknown. They might include autoimmune disorders. A variety of...

Preliminary evaluation of LATEX/T. b. gambiense and alternative versions of CATT/T. b. gambiense for the serodiagnosis of human African trypanosomiasis of a population at risk in Côte d'Ivoire: considerations for mass-screening (2000)

Jamonneau, V., Truc, P., Garcia, A., Magnus, E., Büscher, P.

A study was conducted to compare classical card agglutination test for trypanosomiasis (CATT)/T. b. gambiense with CATT-EDTA and LATEX/T. b. gambiense as alternative field tests for serodiagnosis of...

Comparative in vitro isolation of Trypanosoma theileri from cattle in Belgium (2000)

Verloo, D., Brandt, J., Van Meirvenne, N., Büscher, P.

Ten blood samples randomly collected from cows on a farm nearby Antwerp, Belgium, were inoculated into KIVI culture medium (Kit for In Vitro Isolation of trypanosomes) and RPMI 10%+feeder medium....

Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam (2000)

Verloo, D., Holland, W., My, L. N., Thanh, N. G., Tam, P. T., Goddeeris, B., ...

In the present study, a collection of 415 water buffalo serum samples originating from the north of Vietnam was used for evaluation of different diagnostic antibody detection methods available to...

Validation of a PCR-Oligochromatography Test for Detection of Trypanozoon Parasites in a Multicenter Collaborative Trial▿

Claes, F., Deborggraeve, S., Verloo, D., Mertens, P., Crowther, J. R., Leclipteux, T., ...

A PCR-oligochromatography test for diagnosis of human and animal trypanosomiasis was evaluated through a multicenter ring trial with six laboratories testing a set of 21 blinded samples, resulting in...

Human African trypanosomiasis: a latex agglutination field test for quantifying IgM in cerebrospinal fluid.

Lejon, V., Büscher, P., Sema, N. H., Magnus, E., Van Meirvenne, N.

LATEX/IgM, a rapid agglutination test for the semi-quantitative detection of IgM in cerebrospinal fluid of patients with African trypanosomiasis, is described in this article. The lyophilized reagent...