Phase-locking of two self-seeded tapered amplofier lasers (2010)
Tackmann, G., Gilowski, M., Schubert, Ch., Berg, P., Wendrich, T., Ertmer, W., ...
We report on the phase-locking of two diode lasers based on self-seeded tapered amplifiers. In these lasers, a reduction of linewidth is achieved using narrow-band high-transmission interference...
A comparison in cardiac output data: a random effects model for repeated measures (2010)
De Wilde, R, Geerts, B, Berg, P, Jansen, J
No abstract available.
Continuous cardiac output measurement: effect of time (2010)
De Wilde, R, Geerts, B, Berg, P, Jansen, J
No abstract available.
Comparison of cardiac output values; pulse contour methods against thermodilution technique (2010)
De Wilde, R, Geerts, B, Berg, P, Jansen, J
No abstract available.
Binnekade, J, Wilde, R, Slooter, AJ, Sluijs, J, Beenen, O, Schultz, M, ...
No abstract available.
Berg, P., Haerter, J. O., Thejll, P., Piani, C., Hagemann, S., Christensen, J. H.
Past studies have argued that the intensity of extreme precipitation events should increase exponentially with temperature. This argument is based on the principle that the atmospheric moisture...
Berg, P., Haerter, J.O., Thejll, P., Piani, C., Hagemann, S., Christensen, J.H.
Journal of Geophysical Research, 114(D18102), doi:10.1029/2009JD012008, 2009
Unexpected rise in extreme precipitation caused by a shift in rain type? (2009)
Nature Geoscience, 2(2009) S.372-73
S. Schanz, W. Kruis, O. Mickisch, B. Küppers, P. Berg, B. Frick, ...
Background: Adequate bowel preparation is essential for accurate colonoscopy. Both oral sodium phosphate (NaP) and polyethylene glycol-based lavage (PEG-ELS) are used predominantly as bowel cleansing...
Recent Results from Studies of Electric Discharges in the Mesosphere (2008)
Neubert, T., Rycroft, M., Farges, T., Blanc, E., Chanrion, O., Arnone, E., ...
The paper reviews recent advances in studies of electric discharges in the stratosphere and mesosphere above thunderstorms, and their effects on the atmosphere. The primary focus is on the sprite...
An estimate of the impact of transient luminous events on the atmospheric temperature (2008)
E. Arnone, P. Berg, N. F. Arnold, B. Christiansen, P. Thejll
We present an order of magnitude estimate of the impact of sprites and other transient luminous events (TLEs) on the atmospheric temperature via ozone changes. To address the effects of expected...
S. Schanz, W. Kruis, O. Mickisch, B. Küppers, P. Berg, B. Frick, ...
Background: Adequate bowel preparation is essential for accurate colonoscopy. Both oral sodium phosphate (NaP) and polyethylene glycol-based lavage (PEG-ELS) are used predominantly as bowel cleansing...
Relating Car-Following and Continuum Models of Road Traffic (2007)
. We derive a transformation that relates car-following models to their analogous continuum counterpart by using an integral representation of the density. A Taylor expansion of the latter yields an...
Chanrion, O., Crosby, N. B., Arnone, E., Boberg, F., Van Der Velde, O., Odzimek, A., ...
The four year "Coupling of Atmospheric Layers (CAL)" EU FP5 Research Training Network project studied unanswered questions related to transient luminous events (sprites, jets and elves) in the upper...
Chanrion, O., Crosby, N. B., Arnone, E., Boberg, F., Van Der Velde, O., Odzimek, A., ...
The four year "Coupling of Atmospheric Layers (CAL)" EU FP5 Research Training Network project studied unanswered questions related to transient luminous events (sprites, jets and elves) in the upper...
N. C. Friggens, P. Berg, P. Theilgaard, I. R. Korsgaard, K. L. Ingvartsen, P. Løvendahl, ...
The aim of this study was to characterize patterns of energy balance through lactation of cows kept under constant feeding conditions. Danish Holstein, Danish Red, and Jersey cows were studied during...
O. Chanrion, N. B. Crosby, E. Arnone, F. Boberg, O. Van Der Velde, A. Odzimek, ...
The four year "Coupling of Atmospheric Layers (CAL)" EU FP5 Research Training Network project studied unanswered questions related to transient luminous events (sprites, jets and elves) in...
Chanrion, O., Crosby, N. B., Arnone, E., Boberg, F., Van Der Velde, O., Odzimek, A., ...
The four year "Coupling of Atmospheric Layers (CAL)" EU FP5 Research Training Network project studied unanswered questions related to transient luminous events (sprites, jets and elves) in the upper...
Chanrion, O., Crosby, N. B., Arnone, E., Boberg, F., Van Der Velde, O., Odzimek, A., ...
The four year "Coupling of Atmospheric Layers (CAL)" EU FP5 Research Training Network project studied unanswered questions related to transient luminous events (sprites, jets and elves) in the upper...
Nielsen, H.M., Groen, A.F., Ostergaard, S., Berg, P.
The objective of this paper was to present a model of a dairy cattle production system for the derivation of economic values and their standard deviations for both production and functional traits...
Nielsen, H.M., Groen, A.F., Ostergaard, S., Berg, P.
The objective of this paper was to present a model of a dairy cattle production system for the derivation of economic values and their standard deviations for both production and functional traits...
Hansen, B. H., Keller, J., Laitinen, M., Berg, P., Skjeldal, S., Trovik, C., ...
Abstract is not available
Ubbink, D.Th., Vermeulen, H., Spincemaille, G.H.J.J., Gersbach, P.A., Berg, P., Amann, W.
Process models for managing front-end - case studies from finnsih industry (2004)
Poskela, J., Nordlund, H., Perttula, M., Kohn, S., Berg, P.
We are living an era of rapid change. New emerging technologies, shortening product life cycles, more demanding customers, and increasing global competition force companies to be more innovative in...
Berg, P., Pihlajamaa, J., Nummi, J., Seppälä, J., Haner, U.-E.
EDMAN, K., LÖFSTEDT, H., BERG, P., ERIKSSON, K., AXELSSON, S., BRYNGELSSON, I., ...
The main aim of the study was to measure the exposure to monoterpenes (α- and &bgr;-pinene and Δ3-carene) and wood dust during industrial production of wood pellets and briquettes. Additional aims...
A model for derivation of economic values of production and functional traits in dairy cattle (2002)
The Reuven Ramaty high-energy solar spectroscopic imager (RHESSI) (2002)
Lin, R.P., Dennis, B.R., Hurford, G.J., Smith, D.M., Zehnder, A., Harvey, P.R., ...
RHESSI is the sixth in the NASA line of Small Explorer (SMEX) missions and the first managed in the Principal Investigator mode, where the PI is responsible for all aspects of the mission except the...
Picton, T.W., Bentin, S., Berg, P., Donchin, E., Hillyard, S.A., Johnson, R., ...
Event-related potentials (ERPs) recorded from the human scalp can provide important information about how the human brain normally processes information and about how this processing may go awry in...
Eyeblinks evoke potentials in the occipital brain region (1992)
Heuser-Link, M., Dirlich, G., Berg, P., Vogl, L., Scherg, M.
SPINAL ANAESTHESIA IN DAY-CARE SURGERY WITH A 26-GAUGE NEEDLE (1990)
QUAYNOR, H., CORBEY, M., BERG, P.
We studied 106 day-care surgical patients (52 male) aged 18–70 yr (mean 49.6 yr) who received spinal anaesthesia with a 26-gauge spinal needle. The incidence of headache, back pain and patient...
Jolly, D. J., Okayama, H., Berg, P., Esty, A. C., Filpula, D., Bohlen, P., ...
We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus...
Nucleotide-sequence deletions within the coding region for small-t-antigen of simian virus-40 (1979)
Volckaert, Guido; U0006538 JFA; CORA, Feunteun, J, Crawford, Lv, Berg, P, Fiers, W
Synthesis of Superhelical Simian Virus 40 Deoxyribonucleic Acid in Cell Lysates* (1975)
DePamphilis, M. L., Beard, P., Berg, P.
In vivo-labeled SV40 replicating DNA molecules can be converted into covalently closed superhelical SV40 DNA (SV40(I) using a lysate of sv40-infected monkey cells containing intact nuclei....
Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease (1973)
Beard, P., Morrow, J. F., Berg, P.
S(1) nuclease, the single-strand specific nuclease from Aspergillus oryzae can cleave both strands of circular covalently closed, superhelical simian virus 40 (SV40) DNA to generate unit length...
The Reuven Ramaty high-energy solar spectroscopic imager (RHESSI)
Lin, R.P., Dennis, B.R., Hurford, G.J., Smith, D.M., Zehnder, A., Harvey, P.R., ...
RHESSI is the sixth in the NASA line of Small Explorer (SMEX) missions and the first managed in the Principal Investigator mode, where the PI is responsible for all aspects of the mission except the...
Hays, S L, Firmenich, A A, Berg, P
The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members. Here, we show that the x-ray sensitivity of rad55...
Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells.
Gassmann, M, Donoho, G, Berg, P
We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid...
Luria, S, Chambers, I, Berg, P
Stable transformants of the Jurkat T-cell line have been obtained that express either of two distinct forms of the type 1 human immunodeficiency virus nef gene: the nef-1-encoded protein (Nef-1)...
The rate of renaturation for complementary DNA strands can be enhanced greater than 10(4)-fold by the addition of simple cationic detergents, and the reaction is qualitatively and quantitatively very...
Purified heterogeneous nuclear ribonucleoprotein (hnRNP) A1 protein, which is found in vivo associated with heterogeneous nuclear RNA (hnRNA), promotes the rapid renaturation of nucleic acid strands....
Firmenich, A A, Elias-Arnanz, M, Berg, P
To understand the mechanisms involved in homologous recombination, we have performed a search for Saccharomyces cerevisiae mutants unable to carry out plasmid-to-chromosome gene conversion. For this...
Suppression of mutations in two Saccharomyces cerevisiae genes by the adenovirus E1A protein.
Zieler, H A, Walberg, M, Berg, P
The protein products of the adenoviral E1A gene are implicated in a variety of transcriptional and cell cycle events, involving interactions with several proteins present in human cells, including...
Characterization of strand exchange activity of yeast Rad51 protein.
The Saccharomyces cerevisiae RAD51 gene product takes part in genetic recombination and repair of DNA double strand breaks. Rad51, like Escherichia coli RecA, catalyzes strand exchange between...
Transduction of cellular neo mRNA by retrovirus-mediated recombination.
Stuhlmann, H, Dieckmann, M, Berg, P
Transduction of cellular oncogenes by retroviruses is thought to be a multistep process, involving transcriptional activation of a cellular gene by upstream proviral integration and joining of...
White, R T, Berg, P, Villarreal, L P
Deletions were introduced at exon-intron boundaries in the late region of a simian virus 40-beta-globin cDNA recombinant to study the role of splicing in the formation of simian virus 40 late...
Homologous recombination of copackaged retrovirus RNAs during reverse transcription.
According to prevailing models, the high frequency of recombination in retroviruses occurs during reverse transcription of two genetically different genomes copackaged into virion particles. This...
Cultured monkey (TC7) and mouse (3T6) cells synthesize an Excherichia coli enzyme, xanthine-guanine phosphoribosyltransferase (XGPRT; 5-phospho-alpha-D-ribose-1-diphosphate:xanthine...
Transcription from a plant gene promoter in animal cells.
The promoter segment of a plant gene (maize alcohol dehydrogenase 1 (Adh 1)) has been fused to two bacterial reporter genes, Ecogpt (1) and neo (2), in pSV2-derived vectors and introduced into...
Small patches of identical amino acid sequences commonly occur in proteins that have the same function but are derived from evolutionarily distant organisms. Reverse translation of such patches into...
Electroporation for the efficient transfection of mammalian cells with DNA.
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are...
Cultured mammalian cells transduced with the Escherichia coli gene, Ecogpt, synthesize the bacterial enzyme xanthine-guanine phosphoribosyl transferase (XGPT) (1). This paper describes a method for...
The human interferon beta 1 gene has been inserted into simian virus 40 hybrid plasmid vectors carrying the bacterial phosphotransferase gene (neo), and introduced into cultured mammalian cells by...
Villarreal, L P, White, R T, Berg, P
We have analyzed the structure of the late cytoplasmic RNAs made after infection with wild-type simian virus 40 and a set of viable mutants, four of which have deletions and one an insertion within...
Contreras, R, Cole, C, Berg, P, Fiers, W
Two mutants of simian virus 40, dl-1261 and dl-1262, have deletions that map between coordinated 0.90 and 0.95 (Cole et al., J. Virol 24:277--294, 1977). Both affect the structure of the two minor...
Nucleotide sequence deletions within the coding region for small-t antigen of simian virus 40.
Volckaert, G, Feunteun, J, Crawford, L V, Berg, P, Fiers, W
Simian virus 40 early mutants with deletions mapping in the 0.53-0.60 region have been sequenced by the Maxam and Gilbert approach. All these deletions effect the small-t gene. The size of the...
Van Heuverswyn, H, Cole, C, Berg, P, Fiers, W
Nucleotide sequence analysis of two simian virus 40 early mutants dl1263 and dl1265, which lack a DNA segment around map positions 0.21 and 0.18, respectively (C. Cole, T. Landers, S. Goff, S....
Construction and analysis of viable deletion mutants of polyoma virus.
Viable mutants of polyoma with small deletions ranging in size from 2 to 75 base pairs were obtained by infecting 3T3 cells with polyoma DNA that had been cleaved once with HaeII endonuclease or with...
Does simian virus 40 DNA integrate into cellular DNA during productive infection?
Late after infection of permissive monkey cells by simian virus 40 (SV40), large amounts of SV40 DNA (30,000 to 220,000 viral genome equivalents per cell) can be isolated with the...
Griffith, J, Dieckmann, M, Berg, P
A salt-stable complex of protein and viral DNA obtained from Simian virus 40 (SV40)-infected monkey cells or mature SV40 virions has a novel structure. When viewed by high resolution electron...
Wilson, J H, DePamphilis, M, Berg, P
A fraction of permissive cells survive simian virus 40 (SV40) infection. The frequency of such surviving cells depends only upon the concentration of infecting virus, both parental and progeny, to...
We have designed an in vitro system using mammalian nuclear extracts, or fractions derived from them, that can restore the sequences missing at double-strand breaks (gaps) or in deletions. The...
Margolskee, R F, Kavathas, P, Berg, P
Efficient transfection and expression of cDNA libraries in human cells has been achieved with an Epstein-Barr virus-based subcloning vector (EBO-pcD). The plasmid vector contains a resistance marker...
The asialoglycoprotein receptor (ASGP-R) from rat liver contains the following three distinct protein species when it is analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: RHL1...
Comparison of intron-dependent and intron-independent gene expression.
Recombinant simian virus 40 viruses carrying rabbit beta-globin cDNA failed to express the beta-globin sequence unless an intron was included in the transcription unit. The addition of either...
Naumovski, L, Chu, G, Berg, P, Friedberg, E C
We determined the complete nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae. The coding region of the gene contained 2,334 base pairs that could encode a protein with a calculated...
Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.
We have developed a bacteriophage lambda vector (lambda NMT) that permits efficient transduction of mammalian cells with a cDNA clone library constructed with the pcD expression vector (H. Okayama...
Mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins M1 and M2, which are differentially regulated during the cell cycle. We have isolated expressible cDNA clones of...
We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus...
Termination-reinitiation occurs in the translation of mammalian cell mRNAs.
Many examples of internal translation initiation in eucaryotes have accumulated in recent years. In many cases terminators of upstream reading frames precede the internal initiation site, suggesting...
Effect of upstream reading frames on translation efficiency in simian virus 40 recombinants.
Peabody, D S, Subramani, S, Berg, P
In a previous report (S. Subramani, R. Mulligan, and P. Berg, Mol. Cell. Biol. 1:854-864, 1981), it was shown that mouse dihydrofolate reductase (DHFR) could be efficiently expressed from simian...
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
This paper describes a plasmid vector for cloning cDNAs in Escherichia coli; the same vector also promotes expression of the cDNA segment in mammalian cells. Simian virus 40 (SV40)-derived DNA...
Tandemly repeated 72-base-pair (bp) segments located between nucleotides 107 and 250 of the simian virus 40 genome are essential for early region transcription. The functional requirement for the...
Homologous and nonhomologous recombination in monkey cells.
Though recombinational events are important for the proper functioning of most cells, little is known about the frequency and mechanisms of recombination in mammalian cells. We have used simian virus...
Buchman, A R, Fromm, M, Berg, P
During simian virus 40 lytic infection there is a shift in initiation sites used to transcribe the early region, which encodes large T and small t antigens. Early in infection, transcription is...
As part of our efforts to create multifunctional vectors for the transduction of animal cells, a set of simian virus 40 recombinants were constructed which contain an inverted duplication of the...
Simian virus 40 minichromosomes contain torsionally strained DNA molecules.
Sundin and Varshavsky (J. Mol. Biol. 132:535-546, 1979) found that nearly two-thirds of simian virus 40 (SV40) minichromosomes obtained from nuclei of SV40-infected cells become singly nicked or...
Factors governing the expression of a bacterial gene in mammalian cells.
Cultured monkey kidney cells transfected with simian virus 40 (SV40)-pBR322-derived deoxyribonucleic acid (DNA) vectors containing the Escherichia coli gene (Ecogpt, or gpt) coding for the enzyme...
Subramani, S, Mulligan, R, Berg, P
A mouse complementary deoxyribonucleic acid segment coding for the enzyme dihydrofolate reductase has been cloned in two general classes of vectors containing simian virus 40 deoxyribonucleic acid:...
High-efficiency cloning of full-length cDNA.
A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure...
The rat asialoglycoprotein receptor (ASGP-R) has been expressed in cultured rat hepatoma cells (HTC cells) after transfection with cloned cDNAs. Fluorescence-activated cell sorting of transfected...
Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.
A method is described for excising cloned DNA segments that have been inserted into their vectors by poly(dA-dT) joins. The recombinant DNA is cleaved within the vector DNA portion by one or more...
New region of the simian virus 40 genome required for efficient viral transformation.
Bouck, N, Beales, N, Shenk, T, Berg, P, Di Mayorca, G
Viable mutants of simian virus 40 with deletions in three regions of the virus genome (map coordinates 0.21-0.17, 0.59-0.54, and 0.67-0.74) have been tested for their ability to transform rat...
Jolly, D J, Okayama, H, Berg, P, Esty, A C, Filpula, D, Bohlen, P, ...
We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus...
Immunoglobulin gene expression in transformed lymphoid cells.
Oi, V T, Morrison, S L, Herzenberg, L A, Berg, P
Myeloma, hybridoma, and thymoma cell lines have been successfully transfected for the Escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt) by using the plasmid vector pSV2-gpt. The...
Organization and expression of early genes of simian virus 40
Crawford, L. V., Cole, C. N., Smith, A. E., Paucha, E., Tegtmeyer, P., Rundell, K., ...
The early region of simian virus 40 codes for at least two immunologically related polypeptides: large-T and small-t, with apparent molecular weights of 90,000-100,000 and 15,000-20,000,...
Heteroduplex DNA molecules formed from two DNAs that differ from each other by a deletion can be cleaved at the mismatched region (a deletion loop) with the single-strand-specific S1 endonuclease. A...
Electron microscopy of gene regulation: the L-arabinose operon.
Unlike normal cells, malignant rat and two simian virus 40-transformed human cell lines can neither grow nor survive in B12- and folate-supplemented media in which methionine is replaced by...
Shenk, T E, Rhodes, C, Rigby, P W, Berg, P
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base...
Biochemical procedure for production of small deletions in simian virus 40 DNA.
Carbon, J, Shenk, T E, Berg, P
A simple biochemical procedure for producing small deletions (15 to 50 base pairs) at virtually any location in simian virus 40 DNA has been developed. The steps involved are: cleavage of the...
Construction and analysis of viable deletion mutants of simian virus 40.
Shenk, T E, Carbon, J, Berg, P
Viable mutants of simian virus 40 (SV40), with deletions ranging in size from 15 to 200 base pairs, have been obtained by infecting CV-1P cells with circularly permuted linear SV40 DNA. The linear...
Restriction endonuclease from Haemophilus gallinarum (HgaI) cleaves polyoma DNA at four locations.
A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the...
Characterization of components released by alkali disruption of simian virus 40.
Christiansen, G, Landers, T, Griffith, J, Berg, P
Treatment of simian virus 40 (SV40) particles at pH 9.8 in the presence of 1 mM dithiothreitol for 5 min at 37 degrees C disrupted the virions into a 60S DNA-protein complex and DNA-free 7S protein...
Structure and formation of circular dimers of simian virus 40 DNA.
Most of the viral DNA extracted from simian virus 40 (SV40)-infected African green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. However, about 1% of the...
Transfer of human and murine globin-gene sequences into transgenic mice.
Humphries, R K, Berg, P, DiPietro, J, Bernstein, S, Baur, A, Nienhuis, A W, ...
We have studied the transfer of human and murine globin gene sequences into fertilized mouse oocytes by microinjection. Germline transmission was demonstrated for the human delta- and beta-globin...
Hays, S L, Firmenich, A A, Berg, P
The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members. Here, we show that the x-ray sensitivity of rad55...
Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells.
Gassmann, M, Donoho, G, Berg, P
We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid...
Luria, S, Chambers, I, Berg, P
Stable transformants of the Jurkat T-cell line have been obtained that express either of two distinct forms of the type 1 human immunodeficiency virus nef gene: the nef-1-encoded protein (Nef-1)...
The rate of renaturation for complementary DNA strands can be enhanced greater than 10(4)-fold by the addition of simple cationic detergents, and the reaction is qualitatively and quantitatively very...
Purified heterogeneous nuclear ribonucleoprotein (hnRNP) A1 protein, which is found in vivo associated with heterogeneous nuclear RNA (hnRNA), promotes the rapid renaturation of nucleic acid strands....
Firmenich, A A, Elias-Arnanz, M, Berg, P
To understand the mechanisms involved in homologous recombination, we have performed a search for Saccharomyces cerevisiae mutants unable to carry out plasmid-to-chromosome gene conversion. For this...
Suppression of mutations in two Saccharomyces cerevisiae genes by the adenovirus E1A protein.
Zieler, H A, Walberg, M, Berg, P
The protein products of the adenoviral E1A gene are implicated in a variety of transcriptional and cell cycle events, involving interactions with several proteins present in human cells, including...
Characterization of strand exchange activity of yeast Rad51 protein.
The Saccharomyces cerevisiae RAD51 gene product takes part in genetic recombination and repair of DNA double strand breaks. Rad51, like Escherichia coli RecA, catalyzes strand exchange between...
Transduction of cellular neo mRNA by retrovirus-mediated recombination.
Stuhlmann, H, Dieckmann, M, Berg, P
Transduction of cellular oncogenes by retroviruses is thought to be a multistep process, involving transcriptional activation of a cellular gene by upstream proviral integration and joining of...
White, R T, Berg, P, Villarreal, L P
Deletions were introduced at exon-intron boundaries in the late region of a simian virus 40-beta-globin cDNA recombinant to study the role of splicing in the formation of simian virus 40 late...
Homologous recombination of copackaged retrovirus RNAs during reverse transcription.
According to prevailing models, the high frequency of recombination in retroviruses occurs during reverse transcription of two genetically different genomes copackaged into virion particles. This...
Cultured monkey (TC7) and mouse (3T6) cells synthesize an Excherichia coli enzyme, xanthine-guanine phosphoribosyltransferase (XGPRT; 5-phospho-alpha-D-ribose-1-diphosphate:xanthine...
Transcription from a plant gene promoter in animal cells.
The promoter segment of a plant gene (maize alcohol dehydrogenase 1 (Adh 1)) has been fused to two bacterial reporter genes, Ecogpt (1) and neo (2), in pSV2-derived vectors and introduced into...
Small patches of identical amino acid sequences commonly occur in proteins that have the same function but are derived from evolutionarily distant organisms. Reverse translation of such patches into...
Electroporation for the efficient transfection of mammalian cells with DNA.
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are...
Cultured mammalian cells transduced with the Escherichia coli gene, Ecogpt, synthesize the bacterial enzyme xanthine-guanine phosphoribosyl transferase (XGPT) (1). This paper describes a method for...
The human interferon beta 1 gene has been inserted into simian virus 40 hybrid plasmid vectors carrying the bacterial phosphotransferase gene (neo), and introduced into cultured mammalian cells by...
Villarreal, L P, White, R T, Berg, P
We have analyzed the structure of the late cytoplasmic RNAs made after infection with wild-type simian virus 40 and a set of viable mutants, four of which have deletions and one an insertion within...
Contreras, R, Cole, C, Berg, P, Fiers, W
Two mutants of simian virus 40, dl-1261 and dl-1262, have deletions that map between coordinated 0.90 and 0.95 (Cole et al., J. Virol 24:277--294, 1977). Both affect the structure of the two minor...
Nucleotide sequence deletions within the coding region for small-t antigen of simian virus 40.
Volckaert, G, Feunteun, J, Crawford, L V, Berg, P, Fiers, W
Simian virus 40 early mutants with deletions mapping in the 0.53-0.60 region have been sequenced by the Maxam and Gilbert approach. All these deletions effect the small-t gene. The size of the...
Van Heuverswyn, H, Cole, C, Berg, P, Fiers, W
Nucleotide sequence analysis of two simian virus 40 early mutants dl1263 and dl1265, which lack a DNA segment around map positions 0.21 and 0.18, respectively (C. Cole, T. Landers, S. Goff, S....
Construction and analysis of viable deletion mutants of polyoma virus.
Viable mutants of polyoma with small deletions ranging in size from 2 to 75 base pairs were obtained by infecting 3T3 cells with polyoma DNA that had been cleaved once with HaeII endonuclease or with...
Does simian virus 40 DNA integrate into cellular DNA during productive infection?
Late after infection of permissive monkey cells by simian virus 40 (SV40), large amounts of SV40 DNA (30,000 to 220,000 viral genome equivalents per cell) can be isolated with the...
Griffith, J, Dieckmann, M, Berg, P
A salt-stable complex of protein and viral DNA obtained from Simian virus 40 (SV40)-infected monkey cells or mature SV40 virions has a novel structure. When viewed by high resolution electron...
Wilson, J H, DePamphilis, M, Berg, P
A fraction of permissive cells survive simian virus 40 (SV40) infection. The frequency of such surviving cells depends only upon the concentration of infecting virus, both parental and progeny, to...
We have designed an in vitro system using mammalian nuclear extracts, or fractions derived from them, that can restore the sequences missing at double-strand breaks (gaps) or in deletions. The...
Margolskee, R F, Kavathas, P, Berg, P
Efficient transfection and expression of cDNA libraries in human cells has been achieved with an Epstein-Barr virus-based subcloning vector (EBO-pcD). The plasmid vector contains a resistance marker...
The asialoglycoprotein receptor (ASGP-R) from rat liver contains the following three distinct protein species when it is analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: RHL1...
Comparison of intron-dependent and intron-independent gene expression.
Recombinant simian virus 40 viruses carrying rabbit beta-globin cDNA failed to express the beta-globin sequence unless an intron was included in the transcription unit. The addition of either...
Naumovski, L, Chu, G, Berg, P, Friedberg, E C
We determined the complete nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae. The coding region of the gene contained 2,334 base pairs that could encode a protein with a calculated...
Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.
We have developed a bacteriophage lambda vector (lambda NMT) that permits efficient transduction of mammalian cells with a cDNA clone library constructed with the pcD expression vector (H. Okayama...
Mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins M1 and M2, which are differentially regulated during the cell cycle. We have isolated expressible cDNA clones of...
We have examined the ability of the simian virus 40 72-base pair enhancer segment to simultaneously activate multiple transcription units with plasmids that contain one, two, or three simian virus...
Termination-reinitiation occurs in the translation of mammalian cell mRNAs.
Many examples of internal translation initiation in eucaryotes have accumulated in recent years. In many cases terminators of upstream reading frames precede the internal initiation site, suggesting...
Effect of upstream reading frames on translation efficiency in simian virus 40 recombinants.
Peabody, D S, Subramani, S, Berg, P
In a previous report (S. Subramani, R. Mulligan, and P. Berg, Mol. Cell. Biol. 1:854-864, 1981), it was shown that mouse dihydrofolate reductase (DHFR) could be efficiently expressed from simian...
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
This paper describes a plasmid vector for cloning cDNAs in Escherichia coli; the same vector also promotes expression of the cDNA segment in mammalian cells. Simian virus 40 (SV40)-derived DNA...
Tandemly repeated 72-base-pair (bp) segments located between nucleotides 107 and 250 of the simian virus 40 genome are essential for early region transcription. The functional requirement for the...
Homologous and nonhomologous recombination in monkey cells.
Though recombinational events are important for the proper functioning of most cells, little is known about the frequency and mechanisms of recombination in mammalian cells. We have used simian virus...
Buchman, A R, Fromm, M, Berg, P
During simian virus 40 lytic infection there is a shift in initiation sites used to transcribe the early region, which encodes large T and small t antigens. Early in infection, transcription is...
As part of our efforts to create multifunctional vectors for the transduction of animal cells, a set of simian virus 40 recombinants were constructed which contain an inverted duplication of the...
Simian virus 40 minichromosomes contain torsionally strained DNA molecules.
Sundin and Varshavsky (J. Mol. Biol. 132:535-546, 1979) found that nearly two-thirds of simian virus 40 (SV40) minichromosomes obtained from nuclei of SV40-infected cells become singly nicked or...
Factors governing the expression of a bacterial gene in mammalian cells.
Cultured monkey kidney cells transfected with simian virus 40 (SV40)-pBR322-derived deoxyribonucleic acid (DNA) vectors containing the Escherichia coli gene (Ecogpt, or gpt) coding for the enzyme...
Subramani, S, Mulligan, R, Berg, P
A mouse complementary deoxyribonucleic acid segment coding for the enzyme dihydrofolate reductase has been cloned in two general classes of vectors containing simian virus 40 deoxyribonucleic acid:...
High-efficiency cloning of full-length cDNA.
A widely recognized difficulty of presently used methods for cDNA cloning is obtaining cDNA segments that contain the entire nucleotide sequence of the corresponding mRNA. The cloning procedure...
The rat asialoglycoprotein receptor (ASGP-R) has been expressed in cultured rat hepatoma cells (HTC cells) after transfection with cloned cDNAs. Fluorescence-activated cell sorting of transfected...
Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.
A method is described for excising cloned DNA segments that have been inserted into their vectors by poly(dA-dT) joins. The recombinant DNA is cleaved within the vector DNA portion by one or more...
New region of the simian virus 40 genome required for efficient viral transformation.
Bouck, N, Beales, N, Shenk, T, Berg, P, Di Mayorca, G
Viable mutants of simian virus 40 with deletions in three regions of the virus genome (map coordinates 0.21-0.17, 0.59-0.54, and 0.67-0.74) have been tested for their ability to transform rat...
Jolly, D J, Okayama, H, Berg, P, Esty, A C, Filpula, D, Bohlen, P, ...
We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus...
Immunoglobulin gene expression in transformed lymphoid cells.
Oi, V T, Morrison, S L, Herzenberg, L A, Berg, P
Myeloma, hybridoma, and thymoma cell lines have been successfully transfected for the Escherichia coli xanthine-guanine phosphoribosyltransferase gene (gpt) by using the plasmid vector pSV2-gpt. The...
Organization and expression of early genes of simian virus 40
Crawford, L. V., Cole, C. N., Smith, A. E., Paucha, E., Tegtmeyer, P., Rundell, K., ...
The early region of simian virus 40 codes for at least two immunologically related polypeptides: large-T and small-t, with apparent molecular weights of 90,000-100,000 and 15,000-20,000,...
Heteroduplex DNA molecules formed from two DNAs that differ from each other by a deletion can be cleaved at the mismatched region (a deletion loop) with the single-strand-specific S1 endonuclease. A...
Electron microscopy of gene regulation: the L-arabinose operon.
Unlike normal cells, malignant rat and two simian virus 40-transformed human cell lines can neither grow nor survive in B12- and folate-supplemented media in which methionine is replaced by...
Shenk, T E, Rhodes, C, Rigby, P W, Berg, P
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base...
Biochemical procedure for production of small deletions in simian virus 40 DNA.
Carbon, J, Shenk, T E, Berg, P
A simple biochemical procedure for producing small deletions (15 to 50 base pairs) at virtually any location in simian virus 40 DNA has been developed. The steps involved are: cleavage of the...
Construction and analysis of viable deletion mutants of simian virus 40.
Shenk, T E, Carbon, J, Berg, P
Viable mutants of simian virus 40 (SV40), with deletions ranging in size from 15 to 200 base pairs, have been obtained by infecting CV-1P cells with circularly permuted linear SV40 DNA. The linear...
Restriction endonuclease from Haemophilus gallinarum (HgaI) cleaves polyoma DNA at four locations.
A restriction endonuclease obtained from Haemophilus gallinarum (hgaI) cleaves polyoma DNA at four specific sites. Using the EcoRI, HindIII, and HpaII endonuclease restriction sites as reference, the...
Characterization of components released by alkali disruption of simian virus 40.
Christiansen, G, Landers, T, Griffith, J, Berg, P
Treatment of simian virus 40 (SV40) particles at pH 9.8 in the presence of 1 mM dithiothreitol for 5 min at 37 degrees C disrupted the virions into a 60S DNA-protein complex and DNA-free 7S protein...
Structure and formation of circular dimers of simian virus 40 DNA.
Most of the viral DNA extracted from simian virus 40 (SV40)-infected African green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. However, about 1% of the...
Transfer of human and murine globin-gene sequences into transgenic mice.
Humphries, R K, Berg, P, DiPietro, J, Bernstein, S, Baur, A, Nienhuis, A W, ...
We have studied the transfer of human and murine globin gene sequences into fertilized mouse oocytes by microinjection. Germline transmission was demonstrated for the human delta- and beta-globin...
Falcioni, F, Pawelec, G, Brattig, N, Schneider, E M, Berg, P, Wernet, P
T-lymphocyte clones derived from populations sensitized to alloantigens in vitro were tested for their regulatory effects on pokeweed mitogen-stimulated immunoglobulin (Ig) secretion. Clones with...
The Reuven Ramaty high-energy solar spectroscopic imager (RHESSI)
Lin, R.P., Dennis, B.R., Hurford, G.J., Smith, D.M., Zehnder, A., Harvey, P.R., ...
RHESSI is the sixth in the NASA line of Small Explorer (SMEX) missions and the first managed in the Principal Investigator mode, where the PI is responsible for all aspects of the mission except the...
Calès, P, Voigt, J J, Payen, J L, Bloom, E, Berg, P, Vinel, J P, ...
The case is reported of a 70 year old man who presented with severe anaemia because of chronic gastrointestinal blood loss. This loss was ascribed to vascular ectasia resembling the gastric antral...
The mitochondrial adenine nucleotide translocator is an antigen in primary biliary cirrhosis.
Schultheiss, H P, Berg, P, Klingenberg, M
Circulating antibodies reacting specifically with the adenine nucleotide translocator from liver mitochondria were detected in sera from 12 patients with proven primary biliary cirrhosis (PBC) by a...
Objective—To study attitudes towards and use of bicycle helmets among schoolchildren; to determine whether these attitudes are associated with the involvement of parents and school in bike safety.
Schanz, S., Kruis, W., Mickisch, O., Küppers, B., Berg, P., Frick, B., ...
Background: Adequate bowel preparation is essential for accurate colonoscopy. Both oral sodium phosphate (NaP) and polyethylene glycol-based lavage (PEG-ELS) are used predominantly as bowel cleansing...