Shimshek, D.R., Bus, T., Kim, J., Mihaljevic, A., Mack, V., Seeburg, P.H., ...
Genetic perturbations of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors (AMPARs) are widely used to dissect molecular mechanisms of sensory coding, learning, and memory. In this study,...
SVC: structured visualization of evolutionary sequence conservation (2005)
Roepcke, Stefen, Fiziev, P., Seeburg, P. H., Vingron, Martin
We have developed a web application for the detailed analysis and visualization of evolutionary sequence conservation in complex vertebrate genes. Given a pair of orthologous genes, the...
SVC: structured visualization of evolutionary sequence conservation (2005)
S. Roepcke, P. Fiziev, P. H. Seeburg, M. Vingron
We have developed a web application for the detailed analysis and visualization of evolutionary sequence conservation in complex vertebrate genes. Given a pair of orthologous genes, the...
Critical AMPA receptor properties in olfactory and spatial memory (2005)
Brighton Sussex England, Pierre J. Magistretti, P. H. Seeburg, R. Sprengel, D. Shimshek, A. Schaefer, ...
Cellular bases of neurometabolic coupling and its relevance for functional brain imaging.
Critical AMPA receptor properties in olfactory and spatial memory (2005)
Brighton Sussex England, Pierre J. Magistretti, P. H. Seeburg, R. Sprengel, D. Shimshek, A. Schaefer, ...
Cellular bases of neurometabolic coupling and its relevance for functional brain imaging.
SVC: structured visualization of evolutionary sequence conservation (2005)
Roepcke, S., Fiziev, P., Seeburg, P. H., Vingron, M.
We have developed a web application for the detailed analysis and visualization of evolutionary sequence conservation in complex vertebrate genes. Given a pair of orthologous genes, the...
Codon-improved Cre recombinase (iCre) expression in the mouse (2002)
Shimshek, D. R., Kim, J., Hübner, M. R., Spergel, D. J., Buchholz, Frank, Casanova, E., ...
By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre...
Krestel, H. E., Mayford, M., Seeburg, P. H., Sprengel, R.
Doxycycline (Dox)-sensitive co-regulation of two transcriptionally coupled transgenes was investigated in the mouse. For this, we generated four independent mouse lines carrying coding regions for...
A standardized nomenclature for adenosine deaminases that act on RNA (1997)
Bass, B. L., Nishikura, K., Keller, W., Seeburg, P. H., Emeson, R. B., O'Connell, M. A., ...
The distribution of splice variants of the NMDAR1 subunit mRNA in adult rat brain (1995)
Laurie, D. J., Putzke, J., Zieglgänsberger, W., Seeburg, P. H., Tölle, T. R.
Cellular and subcellular distribution of NMDAR1 splice variant mRNA in spinal cord of the rat (1995)
Tölle, T. R., Berthele, A., Laurie, D. J., Seeburg, P. H., Zieglgänsberger, W.
Flip and flop variants of AMPA receptors in the rat lumbar spinal cord (1995)
Tölle, T. R., Berthele, A., Zieglgänsberger, W., Seeburg, P. H., Wisden, W.
Brusa, R., Zimmermann, F., Weiher, H., Karasawa, M., Seeburg, P.H., Sprengel, R.
Cold Spring Harbor Conf.on Mouse Molecular Genetics, Cold Spring Harbor, N.Y., August 30 - September 4, 1994
Differential signal transduction pattern of five splice variants of the PACAP receptor (1993)
Spengler, D., Waeber, C., Pantaloni, C., Holsboer, F., Seeburg, P. H., Bockaert, J., ...
Clarke, I. J., Cummins, J. T., Karsch, Fred J., Seeburg, P. H., Nikolics, K.
The secretion of gonadotropin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) into sheep hypothalamo-hypophyseal portal blood was investigated in ovariectomized ewes. GAP and GnRH were...
Structure and expression of hormone genes (1982)
Baxter, J D, Whitfield, P L, Seeburg, P H, Barta, A, Cooke, N E, Eberhardt, N L, ...
Thyroid hormone receptors and responses (1979)
Baxter, J D, Eberhardt, N L, Apriletti, J W, Johnson, L K, Ivarie, R D, Schachter, B S, ...
Regulation of growth hormone messenger RNA (1979)
Martial, Joseph, Seeburg, P H, Matulich, D T, Goodman, H M, Baxter, J D
In cultured rat pituitary cells, glucocorticoids regulate growth hormone production by modulating the number of growth hormone messenger RNA molecules. The effect is quite specific, since only a few...
Structure and expression in bacteria of growth hormone genes (1979)
GOODMAN, H M, SEEBURG, P H, Martial, Joseph, SHINE, J, ULLRICH, A, BAXTER, J D
Benzon Symposium
Synthesis of growth hormone by bacteria (1978)
Seeburg, P H, Shine, J, Martial, Joseph, Ivarie, R D, Morris, J A, Ullrich, A, ...
A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences...
Regulation of gene expression by thyroid hormones (1978)
LATHAM, K R, MACLEOD, K M, PAPAVASILIOU, S S, Martial, Joseph, SEEBURG, P H, GOODMAN, H M, ...
Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone (1977)
Seeburg, P H, Shine, J, Martial, Joseph, Baxter, J D, Goodman, H M
The primary structure of DNA containing the sequence for rat pituitary growth hormone mRNA has been determined. DNA was obtained by reverse transcription of polyadenylated RNA from cultured pituitary...
Construction and analysis of recombinant DNA for human chorionic somatomammotropin (1977)
Shine, J, Seeburg, P H, Martial, Joseph, Baxter, J D, Goodman, H M
DNA complementary to mRNA coding for the human polypeptide hormone, chorionic somatomammotropin, has been purified by specific restriction endonuclease digestion and religation before cloning into...
Krestel, H. E., Mayford, M., Seeburg, P. H., Sprengel, R.
Doxycycline (Dox)-sensitive co-regulation of two transcriptionally coupled transgenes was investigated in the mouse. For this, we generated four independent mouse lines carrying coding regions for...
Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences.
Herb, A, Higuchi, M, Sprengel, R, Seeburg, P H
RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channel's Q/R...
The third gamma subunit of the gamma-aminobutyric acid type A receptor family.
Herb, A, Wisden, W, Lüddens, H, Puia, G, Vicini, S, Seeburg, P H
Cloned cDNAs encoding a member of the gamma-aminobutyric acid type A receptor gamma-subunit class were isolated from rat-brain-mRNA-derived libraries. The gamma 3 mRNA is present in cortex,...
Recombinantly expressed gamma-aminobutyric acid type A (GABAA) receptors consisting of alpha 1, beta 2, and gamma 2 subunits contain a binding site for benzodiazepines that differs in its properties...
Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse.
Storck, T, Krüth, U, Kolhekar, R, Sprengel, R, Seeburg, P H
Targeting vectors for embryonic stem (ES) cells typically contain a mouse gene segment of >7 kb with the neo gene inserted for positive selection of the targeting event. More complex targeting...
Bach, A W, Lan, N C, Johnson, D L, Abell, C W, Bembenek, M E, Kwan, S W, ...
The monoamine oxidases play a vital role in the metabolism of biogenic amines in the central nervous system and in peripheral tissues. Using oligonucleotide probes derived from three sequenced...
Molecular basis of human growth hormone gene deletions.
Vnencak-Jones, C L, Phillips, J A, Chen, E Y, Seeburg, P H
Crossover sites resulting from unequal recombination within the human growth hormone (GH) gene cluster that cause GH1 gene deletions and isolated GH deficiency type 1A were localized in nine...
Puia, G, Santi, M R, Vicini, S, Pritchett, D B, Seeburg, P H, Costa, E
Cl- currents elicited by gamma-aminobutyric acid (GABA) application were recorded with the whole-cell tight-seal technique from voltage-clamped cortical neurons of neonatal rats in primary culture....
Hjelle, B L, Phillips, J A, Seeburg, P H
It has been shown that the extent of methylation of cytosine in vertebrate DNA is inversely correlated with gene expression. We studied cytosine methylation in and around the homologous human growth...
Adelman, J P, Mason, A J, Hayflick, J S, Seeburg, P H
Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolactin release-inhibiting factor (PIF) were isolated from libraries derived from human and rat...
The human growth hormone gene family: structure and evolution of the chromosomal locus.
Barsh, G S, Seeburg, P H, Gelinas, R E
The structure of the human growth hormone gene cluster has been determined over a 78 kilobase region of DNA by the study of two overlapping cosmids. There are two growth hormone genes interspersed...
Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone.
Singh, A, Chen, E Y, Lugovoy, J M, Chang, C N, Hitzeman, R A, Seeburg, P H
Two genes, MF alpha 1 and MF alpha 2, coding for the alpha-factor in yeast Saccharomyces cerevisiae were identified by in situ colony hybridization of synthetic probes to a yeast genomic library. The...
A system for shotgun DNA sequencing.
Messing, J, Crea, R, Seeburg, P H
A multipurpose cloning site has been introduced into the gene for beta-galactosidase (beta-D-galactosidegalactohydrolase, EC 3.21.23) on the single-stranded DNA phage M13mp2 (Gronenborn, B. and...
The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase.
Hitzeman, R A, Hagie, F E, Hayflick, J S, Chen, C Y, Seeburg, P H, Derynck, R
The DNA sequence of the gene for the yeast glycolytic enzyme, 3-phosphoglycerate kinase (PGK), has been obtained by sequencing part of a 3.1 kbp HindIII fragment obtained from the yeast genome. The...
The sequence of human serum albumin cDNA and its expression in E. coli.
Lawn, R M, Adelman, J, Bock, S C, Franke, A E, Houck, C M, Najarian, R C, ...
A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control...
Detection and partial characterization of proenkephalin mRNA.
Gubler, U, Kilpatrick, D L, Seeburg, P H, Gage, L P, Udenfriend, S
We have used an oligodeoxynucleotide of defined sequence to detect and quantitate proenkephalin mRNA in the poly(A)-containing fraction of RNA from bovine adrenal medullas. The decahexamer...
Molecular basis for familial isolated growth hormone deficiency.
Phillips, J A, Hjelle, B L, Seeburg, P H, Zachmann, M
Nuclear DNA from four individuals with familial isolated growth hormone (somatotropin) deficiency (IGHD) type A was studied by restriction endonuclease analysis. By using 32P-labeled human growth...
Genetic analysis of familial isolated growth hormone deficiency type I.
Phillips, J A, Parks, J S, Hjelle, B L, Herd, J E, Plotnick, L P, Migeon, C J, ...
Nuclear DNA from individuals belonging to nine different families in which two sibs were affected with isolated growth hormone deficiency type I were studied by restriction endonuclease analysis. By...
Roberts, J L, Seeburg, P H, Shine, J, Herbert, E, Baxter, J D, Goodman, H M
A cDNA fragment synthesized from mouse mRNA (ACTH/LPH mRNA) that codes for the precursor polypeptide containing corticotropin (ACTH), beta-lipotropin (LPH), and several other peptides has been cloned...
Gray, P W, Glaister, D, Seeburg, P H, Guidotti, A, Costa, E
Diazepam binding inhibitor (DBI) is a protein that displaces ligands bound to the beta-carboline/benzodiazepine recognition site, an allosteric modulatory site of the type A gamma-aminobutyric acid...
Amino acid sequence of the alpha subunit of transducin deduced from the cDNA sequence.
Medynski, D C, Sullivan, K, Smith, D, Van Dop, C, Chang, F H, Fung, B K, ...
Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs to a family of homologous coupling proteins that also includes Gs and Gi, the regulatory proteins of...
Chakravarti, A, Phillips, J A, Mellits, K H, Buetow, K H, Seeburg, P H
Six restriction fragment length polymorphisms (RFLPs) detected in the human growth hormone-human chorionic somatomammotropin (hGH-hCS) gene cluster were studied in Mediterraneans, Northern Europeans,...
Hayflick, J, Seeburg, P H, Ohlsson, R, Pfeifer-Ohlsson, S, Watson, D, Papas, T, ...
Avian carcinoma virus OK10 has the genetic structure gag-delta pol-myc-delta env. It shares the transformation-specific myc sequence with three other avian carcinoma viruses (MC29, MH2, CMII) and...
Schofield, P R, McFarland, K C, Hayflick, J S, Wilcox, J N, Cho, T M, Roy, S, ...
A purified opioid-binding protein has been characterized by cDNA cloning. The cDNA sequence predicts an extracellularly located glycoprotein of 345 amino acids. This protein does not possess a...
GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.
Ymer, S, Schofield, P R, Draguhn, A, Werner, P, Köhler, M, Seeburg, P H
Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second...
The dopamine D2 receptor: two molecular forms generated by alternative splicing.
Dal Toso, R, Sommer, B, Ewert, M, Herb, A, Pritchett, D B, Bach, A, ...
Cloned human dopamine D2 receptor cDNA was isolated from a pituitary cDNA library and found to encode an additional 29 amino acid residues in the predicted intracellular domain between transmembrane...
Structure of genes for human growth hormone and chorionic somatomammotropin.
Fiddes, J C, Seeburg, P H, DeNoto, F M, Hallewell, R A, Baxter, J D, Goodman, H M
A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin...
Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.
Martial, J A, Baxter, J D, Goodman, H M, Seeburg, P H
Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells....
Martial, J A, Seeburg, P H, Guenzi, D, Goodman, H M, Baxter, J D
Cultured rat pituitary cells (GC) respond to thyroid and glucocorticoid hormones by increases in growth hormone production and growth hormone mRNA. When these cells are transferred from medium...
Voigt, M M, Laurie, D J, Seeburg, P H, Bach, A
To date, there have been at least eight different receptors for the neurotransmitter serotonin (5-HT) identified in the central nervous system. These receptors fall into four pharmacological classes:...
Structure and functional expression of cloned rat serotonin 5HT-2 receptor.
Pritchett, D B, Bach, A W, Wozny, M, Taleb, O, Dal Toso, R, Shih, J C, ...
A complementary DNA (cDNA) encoding a serotonin receptor with 51% sequence identity to the 5HT-1C subtype was isolated from a rat brain cDNA library by homology screening. Transient expression of the...
Shivers, B D, Hilbich, C, Multhaup, G, Salbaum, M, Beyreuther, K, Seeburg, P H
The cloned cDNA encoding the rat cognate of the human A4 amyloid precursor protein was isolated from a rat brain library. The predicted primary structure of the 695-amino acid-long protein displays...
Structural and functional characterization of the gamma 1 subunit of GABAA/benzodiazepine receptors.
Ymer, S, Draguhn, A, Wisden, W, Werner, P, Keinänen, K, Schofield, P R, ...
The GABAA receptor gamma 1 subunit of human, rat and bovine origin was molecularly cloned and compared with the gamma 2 subunit in structure and function. Both gamma subunit variants share 74%...
A glutamate receptor channel with high affinity for domoate and kainate.
Sommer, B, Burnashev, N, Verdoorn, T A, Keinänen, K, Sakmann, B, Seeburg, P H
The non-NMDA family of glutamate receptors comprises a growing number of structurally related subunits (GluR-A to -D or -1 to -4; GluR-5, -6; KA-1). GluR-A to -D appear to constitute the major AMPA...
The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor.
Johnsson, A, Heldin, C H, Wasteson, A, Westermark, B, Deuel, T F, Huang, J S, ...
The relationship between platelet-derived growth factor (PDGF) and the proto-oncogene c-sis has been determined by amino acid sequence analysis of PDGF and nucleotide sequence analysis of c-sis...
SVC: structured visualization of evolutionary sequence conservation
Roepcke, S., Fiziev, P., Seeburg, P. H., Vingron, M.
We have developed a web application for the detailed analysis and visualization of evolutionary sequence conservation in complex vertebrate genes. Given a pair of orthologous genes, the...
A standardized nomenclature for adenosine deaminases that act on RNA.
Bass, B L, Nishikura, K, Keller, W, Seeburg, P H, Emeson, R B, O'Connell, M A, ...
Grenningloh, G, Schmieden, V, Schofield, P R, Seeburg, P H, Siddique, T, Mohandas, T K, ...
Two cDNAs encoding variants (alpha 1 and alpha 2) of the strychnine binding subunit of the inhibitory glycine receptor (GlyR) were isolated from a human fetal brain cDNA library. The predicted amino...
Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes.
Kavanaugh, M P, Christie, M J, Osborne, P B, Busch, A E, Shen, K Z, Wu, Y N, ...
Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100...
Wollmuth, L P, Kuner, T, Seeburg, P H, Sakmann, B
1. The molecular determinants for the narrow constriction of recombinant N-methyl-D-aspartate (NMDA) receptor channels composed of wild-type and mutant NR1- and NR2A-subunits were studied in Xenopus...
1. Tyrosine kinases regulate NMDA receptor-channel activity in cultured neurons, and NMDA receptor subunits are tyrosine phosphorylated in the brain. 2. Heteromeric NMDA receptor-channels were...
Krestel, H. E., Mayford, M., Seeburg, P. H., Sprengel, R.
Doxycycline (Dox)-sensitive co-regulation of two transcriptionally coupled transgenes was investigated in the mouse. For this, we generated four independent mouse lines carrying coding regions for...
Q/R site editing in kainate receptor GluR5 and GluR6 pre-mRNAs requires distant intronic sequences.
Herb, A, Higuchi, M, Sprengel, R, Seeburg, P H
RNA editing by adenosine deamination in brain-expressed pre-mRNAs for glutamate receptor (GluR) subunits alters gene-specified codons for functionally critical positions, such as the channel's Q/R...
The third gamma subunit of the gamma-aminobutyric acid type A receptor family.
Herb, A, Wisden, W, Lüddens, H, Puia, G, Vicini, S, Seeburg, P H
Cloned cDNAs encoding a member of the gamma-aminobutyric acid type A receptor gamma-subunit class were isolated from rat-brain-mRNA-derived libraries. The gamma 3 mRNA is present in cortex,...
Recombinantly expressed gamma-aminobutyric acid type A (GABAA) receptors consisting of alpha 1, beta 2, and gamma 2 subunits contain a binding site for benzodiazepines that differs in its properties...
Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse.
Storck, T, Krüth, U, Kolhekar, R, Sprengel, R, Seeburg, P H
Targeting vectors for embryonic stem (ES) cells typically contain a mouse gene segment of >7 kb with the neo gene inserted for positive selection of the targeting event. More complex targeting...
Bach, A W, Lan, N C, Johnson, D L, Abell, C W, Bembenek, M E, Kwan, S W, ...
The monoamine oxidases play a vital role in the metabolism of biogenic amines in the central nervous system and in peripheral tissues. Using oligonucleotide probes derived from three sequenced...
Molecular basis of human growth hormone gene deletions.
Vnencak-Jones, C L, Phillips, J A, Chen, E Y, Seeburg, P H
Crossover sites resulting from unequal recombination within the human growth hormone (GH) gene cluster that cause GH1 gene deletions and isolated GH deficiency type 1A were localized in nine...
Puia, G, Santi, M R, Vicini, S, Pritchett, D B, Seeburg, P H, Costa, E
Cl- currents elicited by gamma-aminobutyric acid (GABA) application were recorded with the whole-cell tight-seal technique from voltage-clamped cortical neurons of neonatal rats in primary culture....
Hjelle, B L, Phillips, J A, Seeburg, P H
It has been shown that the extent of methylation of cytosine in vertebrate DNA is inversely correlated with gene expression. We studied cytosine methylation in and around the homologous human growth...
Adelman, J P, Mason, A J, Hayflick, J S, Seeburg, P H
Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolactin release-inhibiting factor (PIF) were isolated from libraries derived from human and rat...
The human growth hormone gene family: structure and evolution of the chromosomal locus.
Barsh, G S, Seeburg, P H, Gelinas, R E
The structure of the human growth hormone gene cluster has been determined over a 78 kilobase region of DNA by the study of two overlapping cosmids. There are two growth hormone genes interspersed...
Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromone.
Singh, A, Chen, E Y, Lugovoy, J M, Chang, C N, Hitzeman, R A, Seeburg, P H
Two genes, MF alpha 1 and MF alpha 2, coding for the alpha-factor in yeast Saccharomyces cerevisiae were identified by in situ colony hybridization of synthetic probes to a yeast genomic library. The...
A system for shotgun DNA sequencing.
Messing, J, Crea, R, Seeburg, P H
A multipurpose cloning site has been introduced into the gene for beta-galactosidase (beta-D-galactosidegalactohydrolase, EC 3.21.23) on the single-stranded DNA phage M13mp2 (Gronenborn, B. and...
The primary structure of the Saccharomyces cerevisiae gene for 3-phosphoglycerate kinase.
Hitzeman, R A, Hagie, F E, Hayflick, J S, Chen, C Y, Seeburg, P H, Derynck, R
The DNA sequence of the gene for the yeast glycolytic enzyme, 3-phosphoglycerate kinase (PGK), has been obtained by sequencing part of a 3.1 kbp HindIII fragment obtained from the yeast genome. The...
The sequence of human serum albumin cDNA and its expression in E. coli.
Lawn, R M, Adelman, J, Bock, S C, Franke, A E, Houck, C M, Najarian, R C, ...
A recombinant plasmid has been constructed which contains the mature protein coding region of the human serum albumin (HSA) gene. Bacteria containing this plasmid synthesize HSA protein under control...
Detection and partial characterization of proenkephalin mRNA.
Gubler, U, Kilpatrick, D L, Seeburg, P H, Gage, L P, Udenfriend, S
We have used an oligodeoxynucleotide of defined sequence to detect and quantitate proenkephalin mRNA in the poly(A)-containing fraction of RNA from bovine adrenal medullas. The decahexamer...
Molecular basis for familial isolated growth hormone deficiency.
Phillips, J A, Hjelle, B L, Seeburg, P H, Zachmann, M
Nuclear DNA from four individuals with familial isolated growth hormone (somatotropin) deficiency (IGHD) type A was studied by restriction endonuclease analysis. By using 32P-labeled human growth...
Genetic analysis of familial isolated growth hormone deficiency type I.
Phillips, J A, Parks, J S, Hjelle, B L, Herd, J E, Plotnick, L P, Migeon, C J, ...
Nuclear DNA from individuals belonging to nine different families in which two sibs were affected with isolated growth hormone deficiency type I were studied by restriction endonuclease analysis. By...
Roberts, J L, Seeburg, P H, Shine, J, Herbert, E, Baxter, J D, Goodman, H M
A cDNA fragment synthesized from mouse mRNA (ACTH/LPH mRNA) that codes for the precursor polypeptide containing corticotropin (ACTH), beta-lipotropin (LPH), and several other peptides has been cloned...
Gray, P W, Glaister, D, Seeburg, P H, Guidotti, A, Costa, E
Diazepam binding inhibitor (DBI) is a protein that displaces ligands bound to the beta-carboline/benzodiazepine recognition site, an allosteric modulatory site of the type A gamma-aminobutyric acid...
Amino acid sequence of the alpha subunit of transducin deduced from the cDNA sequence.
Medynski, D C, Sullivan, K, Smith, D, Van Dop, C, Chang, F H, Fung, B K, ...
Transducin, a GTP-binding protein involved in phototransduction in the vertebrate retina, belongs to a family of homologous coupling proteins that also includes Gs and Gi, the regulatory proteins of...
Chakravarti, A, Phillips, J A, Mellits, K H, Buetow, K H, Seeburg, P H
Six restriction fragment length polymorphisms (RFLPs) detected in the human growth hormone-human chorionic somatomammotropin (hGH-hCS) gene cluster were studied in Mediterraneans, Northern Europeans,...
Hayflick, J, Seeburg, P H, Ohlsson, R, Pfeifer-Ohlsson, S, Watson, D, Papas, T, ...
Avian carcinoma virus OK10 has the genetic structure gag-delta pol-myc-delta env. It shares the transformation-specific myc sequence with three other avian carcinoma viruses (MC29, MH2, CMII) and...
Schofield, P R, McFarland, K C, Hayflick, J S, Wilcox, J N, Cho, T M, Roy, S, ...
A purified opioid-binding protein has been characterized by cDNA cloning. The cDNA sequence predicts an extracellularly located glycoprotein of 345 amino acids. This protein does not possess a...
GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.
Ymer, S, Schofield, P R, Draguhn, A, Werner, P, Köhler, M, Seeburg, P H
Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second...
The dopamine D2 receptor: two molecular forms generated by alternative splicing.
Dal Toso, R, Sommer, B, Ewert, M, Herb, A, Pritchett, D B, Bach, A, ...
Cloned human dopamine D2 receptor cDNA was isolated from a pituitary cDNA library and found to encode an additional 29 amino acid residues in the predicted intracellular domain between transmembrane...
Structure of genes for human growth hormone and chorionic somatomammotropin.
Fiddes, J C, Seeburg, P H, DeNoto, F M, Hallewell, R A, Baxter, J D, Goodman, H M
A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin...
Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.
Martial, J A, Baxter, J D, Goodman, H M, Seeburg, P H
Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells....
Martial, J A, Seeburg, P H, Guenzi, D, Goodman, H M, Baxter, J D
Cultured rat pituitary cells (GC) respond to thyroid and glucocorticoid hormones by increases in growth hormone production and growth hormone mRNA. When these cells are transferred from medium...
Voigt, M M, Laurie, D J, Seeburg, P H, Bach, A
To date, there have been at least eight different receptors for the neurotransmitter serotonin (5-HT) identified in the central nervous system. These receptors fall into four pharmacological classes:...
Structure and functional expression of cloned rat serotonin 5HT-2 receptor.
Pritchett, D B, Bach, A W, Wozny, M, Taleb, O, Dal Toso, R, Shih, J C, ...
A complementary DNA (cDNA) encoding a serotonin receptor with 51% sequence identity to the 5HT-1C subtype was isolated from a rat brain cDNA library by homology screening. Transient expression of the...
Shivers, B D, Hilbich, C, Multhaup, G, Salbaum, M, Beyreuther, K, Seeburg, P H
The cloned cDNA encoding the rat cognate of the human A4 amyloid precursor protein was isolated from a rat brain library. The predicted primary structure of the 695-amino acid-long protein displays...
Grenningloh, G, Schmieden, V, Schofield, P R, Seeburg, P H, Siddique, T, Mohandas, T K, ...
Two cDNAs encoding variants (alpha 1 and alpha 2) of the strychnine binding subunit of the inhibitory glycine receptor (GlyR) were isolated from a human fetal brain cDNA library. The predicted amino...
Structural and functional characterization of the gamma 1 subunit of GABAA/benzodiazepine receptors.
Ymer, S, Draguhn, A, Wisden, W, Werner, P, Keinänen, K, Schofield, P R, ...
The GABAA receptor gamma 1 subunit of human, rat and bovine origin was molecularly cloned and compared with the gamma 2 subunit in structure and function. Both gamma subunit variants share 74%...
A glutamate receptor channel with high affinity for domoate and kainate.
Sommer, B, Burnashev, N, Verdoorn, T A, Keinänen, K, Sakmann, B, Seeburg, P H
The non-NMDA family of glutamate receptors comprises a growing number of structurally related subunits (GluR-A to -D or -1 to -4; GluR-5, -6; KA-1). GluR-A to -D appear to constitute the major AMPA...
The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor.
Johnsson, A, Heldin, C H, Wasteson, A, Westermark, B, Deuel, T F, Huang, J S, ...
The relationship between platelet-derived growth factor (PDGF) and the proto-oncogene c-sis has been determined by amino acid sequence analysis of PDGF and nucleotide sequence analysis of c-sis...
Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes.
Kavanaugh, M P, Christie, M J, Osborne, P B, Busch, A E, Shen, K Z, Wu, Y N, ...
Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100...
Wollmuth, L P, Kuner, T, Seeburg, P H, Sakmann, B
1. The molecular determinants for the narrow constriction of recombinant N-methyl-D-aspartate (NMDA) receptor channels composed of wild-type and mutant NR1- and NR2A-subunits were studied in Xenopus...
1. Tyrosine kinases regulate NMDA receptor-channel activity in cultured neurons, and NMDA receptor subunits are tyrosine phosphorylated in the brain. 2. Heteromeric NMDA receptor-channels were...
SVC: structured visualization of evolutionary sequence conservation
Roepcke, S., Fiziev, P., Seeburg, P. H., Vingron, M.
We have developed a web application for the detailed analysis and visualization of evolutionary sequence conservation in complex vertebrate genes. Given a pair of orthologous genes, the...