Scientific Commons: P. J. M. Bonants

A zonal centrifugation method, known as the Hendrickx centrifuge technique, was tested for routine detection of winter sporangia of Synchytrium endobioticum in soil. In four experiments the ability...

A novel technique using the Hendrickx centrifuge for extracting winter sporangia of Synchytrium endobioticum from soil (2007)

Wander, J.G.N., Lamers, J.G., Hendrickx, G., ...

A zonal centrifugation method, known as the Hendrickx centrifuge technique, was tested for routine detection of winter sporangia of Synchytrium endobioticum in soil. In four experiments the ability...

Monitoring gesloten teelt - komkommer : thema innovatie en management - gesloten teelten BO-06-003-003 (2007)

Bonants, P.J.M., Hofland-Zijlstra, J.D., Schoen, C.D.

Beheersing wortelknobbelaaltjes in de biologische kasgroenteteelt : thema innovatie en management - gesloten teelten BO-06-003-010 (2007)

Bonants, P.J.M.

Bodemindicatoren voor het meten van bodemgezondheid : thema effectief en duurzaam middelenpakket BO-06-004-005.002 (2007)

Bonants, P.J.M., Schoen, C.D.

Veldresistentie als preventiestrategie tegen verspreiding van wratziekte (S. endobioticum) : thema: fytosanitair beleid : BO-06-005-001.010 (2007)

Lamers, J.G., Been, T.H., Bonants, P.J.M.

Phytophthora spp. in het wortelmilieu van belangrijke (inheemse) bomen in Nederland (2007)

Bonants, P.J.M., Verstappen, E.C.P., Breeuwsma, S.J.

A TaqMan PCR method for routine diagnosis of the quarantine fungus guignardia citricarpa on citrus fruit (2007)

Kox, L.F.F., Bonants, P.J.M.

With respect to disease risk for the quarantine fungus Guignardia citricarpa on citrus fruit an accurate diagnosis for routine analysis is required. Also, when inspections have to be performed on...

Diagnostic values and utility of immunological, morphological, and molecular methods for in planta detection of phytophthora ramorum (2007)

Kox, L.F.F., Bonants, P.J.M.

In this study, six methods for the detection of Phytophthora ramorum in planta were compared using naturally infested rhododendron plant material. The methods included two immunological methods, one...

Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays¿ (2007)

Szemes, M., Bonants, P.J.M., Kowalchuk, G.A., Salles, J.F., Ortenberg, E., ...

Background Diagnostics and disease-management strategies require technologies to enable the simultaneous detection and quantification of a wide range of pathogenic microorganisms. Most multiplex,...

Beheersing van Phytophthora ramorum : Eindrapportage PT-11685 (2006)

Kessel, G.J.T., Kuik, F. Van, Bonants, P.J.M., Breeuwsma, S.J., Verstappen, E.C.P.

Microsatellite markers identify three lineages of Phytophthora ramorum in US nurseries, yet single lineages in US forest and European nursery populations (2006)

Ivors, K.L., Garbelotto, M., Ruyter, C.P., Bonants, P.J.M., ...

Analysis of 12 polymorphic simple sequence repeats identified in the genome sequence of Phytophthora ramorum, causal agent of 'sudden oak death', revealed genotypic diversity to be significantly...

Scan spoort meer `daders tegelijk op (2006)

Bonants, P.J.M.

Use of hybridization melting kinetics for detecting Phytophthora species using three dimensional microarrays (2006)

Anderson, N.M., Szemes, M., O'Brien, P.A., Schoen, C.D., Boender, P.J., ...

Microarray-based detection is limited by variable and inconsistent hybridization intensities across the diversity of probes used in each array. In this paper, we introduce a novel concept for the...

Monitoring gesloten teelt - komkommer : thema: innovatie & management gesloten teelten (2006)

Bonants, P.J.M., Wubben, J.P.

Monitoren van schimmelsporen : thema effectief en duurzaam middelenpakket BO-06-004 (2006)

Köhl, J., Amsing, J.J., Schoen, C.D., Bonants, P.J.M., Bergervoet, J.H.W., ...

Bodemindicatoren voor het meten van bodemgezondheid : thema: effectief en duurzaam middelenpakket BO-06-004 2.5.2 (2006)

Bonants, P.J.M., Schoen, C.D.

Phytophthora spp. in het wortelmilieu van belangrijke (inheemse) bomen in Nederland (2006)

Bonants, P.J.M.

From single to multiplex detection of plant pathogens: pUMA, a new concept of multiplex detection using microarrays (2005)

Bonants, P.J.M., Schoen, C.D., Szemes, M., Klerks, M.M., ...

Diagnostic application of Padlock Probes - Multiplex Detection of Plant Pathogens using universal microarray (2005)

Szemes, M., Bonants, P.J.M., Baner, J., Landegren, U., Schoen, C.D.

Padlock probes (PLPs) are long oligonucleotides, whose ends are complementary to adjacent target sequences. Upon hybridization to the target, the two ends are brought into contact, allowing PLP...

Novel molecular and biochemical techniques for quality control and monitoring in the agrofood production chain (2005)

Schoen, C.D., Szemes, M., Bonants, P.J.M., Klerks, M.M., ...

Toepassingen van het pUMA systeem voor detectie van meerdere plantpathogenen in grond, water en lucht via een enkele toets (2005)

Bonants, P.J.M., Szemes, M., Zijlstra, C., Schoen, C.D.

Development of PCR-based detection methods for the quarantine phytopathogen Synchytrium endobioticum, causal agent of wart disease (2005)

Bonants, P.J.M., Wander, J.G.N., Lévesque, C.A., ...

Abstract PCR-based methods were developed for the detection and quantification of the potato pathogen Synchytrium endobioticum in soil extracts and in planta. PCR primers, based on the internal...

Patent: Multiplex nucleic acid detection (2005)

Schoen, C.D., Szemes, M., Bonants, P.J.M.

Beheersmaatregelen Phytophthora ramorum (2005)

Kessel, G.J.T., Verstappen, E.C.P., Bonants, P.J.M.

Genetic variation between Phytophthora cactorum isolates differing in their ability to cause crown rot in strawberry (2004)

Eikemo, H., Klemsdal, S.S., Riisberg, I., Bonants, P.J.M., Stensvand, A., Tronsmo, A.M.

Analysis of 44 isolates of Phytophthora cactorum, isolated from strawberry and other hosts, by AFLP showed that the crown rot pathotype is different from leather rot isolates and from P. cactorum...

AFLP and phylogenetic analyses of North American and European populations of Phytophthora ramorum (2004)

Ivors, K.L., Hayden, K.J., Bonants, P.J.M., Rizzo, D.M., Garbelotto, M.

The genetic structure within and between USA and European populations of the emerging phytopathogen Phytophthora ramorum was examined. Four primer combinations were used for amplified fragment length...

A Rapid Diagnostic Test to Distinguish Between American and European Populations of Phytophthora ramorum (2004)

Verstappen, E.C.P., Kox, L.F.F., Flier, W.G., Bonants, P.J.M.

Phylogenetic Analysis of Phytophthora Species Based on Mitochondrial and Nuclear DNA Sequences (2004)

Bakker, F.T., Bonants, P.J.M., Flier, W.G.

A molecular phylogenetic analysis of the genus Phytophthora was performed, 113 isolates from 48 Phytophthora species were included in this analysis. Phylogenetic analyses were performed on regions of...

A combination of baiting and different PCR formats, including measurement of real-time quantitative fluorescence, for the detection of Phytophthora fragariae in strawberry plants (2004)

Bonants, P.J.M., Hooftman, R., Cooke, D.E.L., Guy, D.C., Duncan, J.M.

Phytophthora fragariae, the cause of strawberry red stele disease, is a quarantine pathogen in Europe. Detecting low levels of infection requires sensitive and specific methods. In the past, Dutch...

From single to multiplex detection of plant pathogens by micro-arrays (2004)

Bonants, P.J.M., Schoen, C.D., Szemes, M., Klerks, M.M., ...

Development and validation of a fast PCR-based detection method for pathogenic isolates of the citrus black spot fungus, Guignardia citricarpa (2003)

Bonants, P.J.M., Carroll, G.C., Baayen, R.P.

Based on the ITS regions of the ribosomal DNA, specific primer sets were developed for the citrus pathogen Guignardia citricarpa and the common citrus endophyte, G. mangiferae, and tested for their...

Development of specific primers for detection and identification of Alternaria spp. In carrot material by PCR and comparison with blotter and plating assays (2002)

Konstantinova, P., Bonants, P.J.M.

A combined amplified fragment length polymorphism and randomly amplified polymorphism DNA genetic linkage map of Mycosphaerella graminicola, the Septoria tritici leaf blotch pathogen of wheat (2002)

Kema, G.H.J., Goodwin, S.B., Hamza, S., Verstappen, E.C.P., Cavaletto, J.R., ...

Nonpathogenic Isolates of the Citrus Black Spot Fungus, Guignardia citricarpa, Identified as a Cosmopolitan Endophyte of Woody Plants, Guignardia mangiferae (Phyllosticta capitalensis) (2002)

Baayen, R.P., Bonants, P.J.M., Verkley, G.J.M., Carroll, G.C., ...

Phytophthora ipomoeae, a new homothallic species causing leaf blight on Ipomoea longipedunculata in the Toluca Valley of central Mexico (2002)

Flier, W.G., Grunwald, N.J., Garay-Serrano, E., Lozoya-Saldan, H., ...

A Phytophthora species was found on blighted foliage of Ipomoea longipedunculata, a morning glory native to the highlands of central Mexico. Based on host range, morphology, allozymes, mitochondrial...

Bodemgezondheids-chip: Het meten van de intrinsieke weerbaarheid in de bodem als hulpmiddel bij duurzaam landbouwmanagement (2002)

Schoen, C.D., Zijlstra, C., Bonants, P.J.M.

Ontwikkeling van technieken voor detectie van plantgerelateerde organismen ten dienste van een veilige en duurzame land- en tuinbouw (2002)

Zijlstra, C., Bonants, P.J.M., Schoen, C.D.

Population dynamics of Pythium aphanidermatum in cucumber grown in closed systems (2001)

Postma, J., Bonants, P.J.M.

Phytophthora ramorum sp. nov., a new pathogen on Rhododendron and Viburnum (2001)

Werres, S., Marwitz, R., Bonants, P.J.M., ...

Since 1993, a hitherto unidentified Phytophthora species has been found associated with twig blight disease in Rhododendron and, sporadically, Viburnum. The morphology and growth characteristics of...

Developments in Detection of Plant Pathogens and Plant Related Organisms: Detection in the Past towards Detection in the Future. Proceedings Symposium in Gent (2001)

Bonants, P.J.M., Schoen, C.D., Zijlstra, C.

Alle ziekten op één DNA-chip (2001)

Bonants, P.J.M., Wubben, J.P.

Universele test op alle ziekteverwekkers in ontwikkeling. Voor diverse virussen, bacteriën, schimmels en nematoden zijn snelle tests ontwikkeld met behulp van de PCR-techniek (Polymerase Chain...

Alle ziektes in de glastuinbouw op één DNA-chip (2001)

Bonants, P.J.M., Wubben, J.P., Kamminga, H.

Onderzoekers van Plant Research International in Wageningen en het Praktijkonderzoek Plant en Omgeving hebben nieuwe detectiemethoden ontwikkeld op basis van moleculair-biologische technieken. Voor...

Fusarium redolens f.sp. asparagi, causal agent of asparagus root rot, crown rot and spear rot (2000)

Baayen, R.P., Bonants, P.J.M., Poll, J.T.K., Blok, W.J., Waalwijk, C.

Molecular characterization of natural hybrids of Phytophthora nicotianae and P. cactorum (2000)

Bonants, P.J.M., Baayen, R.P.

Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and nonmonophyletic formae speciales causing wilt and rot disease (2000)

Baayen, R.P., O'Donnell, K., Bonants, P.J.M., Cigelnik, E., Roebroeck, E.J.A., ...

Genetics of biological and molecular markers in Mycosphaerella graminicola, the cause of Septoria tritici leaf blotch of wheat (1999)

Kema, G.H.J., Verstappen, E.C.P., Waalwijk, C., Bonants, P.J.M., ...

Mapping the Mycosphaerella graminicola genome (1999)

Kema, G.H.J., Goodwin, S.B., Hamza, S., Verstappen, E.C.P., Cavaletto, J.R., ...

Identificatie en detectie van pathogene Rhizoctonia solani in bloemkool (1999)

Bonants, P.J.M., Luttikholt, A.J.G.

Phylogeny of VCGs and formae speciales of Fusarium oxysporum and F. redolens (1999)

Baayen, R.P., O'Donnell, K., Waalwijk, C., Bonants, P.J.M., Cigelnik, E., Roebroeck, E.J.A.

Corcordant phylogenies from gene genealogies (mtSSUrDNA; EF-1(), AFLP analyses reveal a polyphyletic nature for formae speciales in Fusarium oxysporum (1999)

Baayen, R.P., O'Donnell, K., Waalwijk, C., Bonants, P.J.M., Cigelnik, E.

Een koppelingskaart van het Mycosphaerella graminicola [anamorph Septoria tritici] genoom. (1998)

Kema, G.H.J., Hamza, S., Bonants, P.J.M., Waalwijk, C., Verstappen, E.C.P., ...

The first linkage map of Mycosphaerella graminicola (anamorph Septoria tritici), Europe's major fungal wheat pathogen. (1998)

Kema, G.H.J., Hamza, S., Bonants, P.J.M., Waalwijk, C., Verstappen, E.C.P., ...

Genetic diversity of Ralstonia solanacearum race 3 in Western Europe determined by AFLP, RC-PFGE and Rep-PCR (1998)

Bonants, P.J.M., Smith, J.J., Hagenaar, M., Nijhuis, E., ...

Schimmels in water opsporen (1998)

Bonants, P.J.M.

Natural hybrids of Phytophthora nicotianae and Phytophthora cactorum demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA (1998)

Veenbaas-Rijks, W.J., Ilieva, E., Bonants, P.J.M., Pieters, R.

Molecular diagnosis of Fusarium spp (1997)

Waalwijk, C., Bonants, P.J.M., Baayen, R.P.

The use of nested PCR in the polymerase chain reaction for the detection of Phytophthora fragariae and P. cactorum in strawberry, pp. 829-838 (1997)

Lacourt, I., Bonants, P.J.M., Cooke, D.E.L., Surplus, L., ...

Phytophthora fragariae var. fragariae Hickman, which causes red core disease of strawberry, is a quarantine organism on which a nil tolerance is placed. Detection of the fungus is by a root tip bait...

Identification of race-specific markers of Phytophthora fragariae with AFLP, pp. 199-201 (1997)

Bonants, P.J.M., Cooke, D.E.L., Duncan, J.M.

The use of nested PCR for the detection of Phytophthora fragariae in strawberry, pp. 43-45 (1997)

Bonants, P.J.M., Cooke, D.E.L., Lacourt, I., Duncan, J.M.

De fysiologische rol van toxinen in de interactie tussen micro-organismen en nematoden (1995)

Bonants, P.J.M.

Detectie van Phytophthora fragariae in aardbei (1995)

Bonants, P.J.M.

The use of nested primers in PCR for the detection of Phytophthora fragariae in strawberry (1995)

Bonants, P.J.M.

A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne hapla eggs (1995)

Bonants, P.J.M., Fitters, P.F.L., Thijs, H., Den Belder, E., Waalwijk, C.