Role of co-stimulation in CD8+ T cell activation (1998)
Pardigon, N, Bercovici, N, Calbo, S, Santos-Lima, EC, Liblau, R, Kourilsky, P, ...
Martinez-Soria, Eduardo, Siegrist, Claire-Anne, Mach, Bernard, Kourilsky, P
Expression of MHC class II, DM and li genes is controlled by the transactivator CIITA, a mediator of the activation of these genes by IFN-γ. Surprisingly, MHC class II molecules expressed on CIITA...
Viret, Christophe, Gervois, Nadine, Guilloux, Yannick, Le Dréan, Eric, Diez, Elisabeth, Jotereau, Francine, ...
B7-1 expression, induced by transfection in poorly Immunogenic murine tumours, was shown to elicit a T cell-mediated rejection of these tumours and further active immunity against the nontransfected...
Kern, Ilse, Steimle, Viktor, Siegrist, Claire-Anne, Mach, Bernard, Kourilsky, P.
MHC-encoded HLA-DMA and-DMB molecules are atypical MHC chains that play an essential role in antigen presentation by MHC class II molecules. They resemble both MHC class I and II molecules but are...
Mutations in residue 61 of H-Ras p21 protein influence MHC class II presentation (1995)
Kayibanda, Michéle, Deprez, Benoit, Levy, Jean-Paul, Guillet, Jean-Gérard, Tilkin, Anne-Françoise, ...
We have investigated the influence of mutations in the Ras 61 codon on the immunogenicity of synthetic peptides and H-Ras p21 proteins. H-2k mice produced Th responses when immunized with mutated...
Rag-1: a topoisomerase? (1993)
Kallenbach, Sacha, Brinkmann, Thomas, Rougeon, François, Kourilsky, P.
Recombination activating genes Rag-1 and Rag-2 were isolated on the basis of their ability to confer V(D)J recombination activity when co-expressed in fibroblasts. The mode of action of the products...
Laouar, Amale, Wietzerbin, Juana, Bauvois, Brigitte, Kourilsky, P.
Taking advantage of the recently demonstrated presence of N-aminopeptidases and the serine protease dipeptidyi aminopeptidase IV (DPP IV) at the surface of human myeloblastic HL-60 cells, the...
Monoclonal antibodies directed against T cell epitopes presented by class I MHC antigens (1993)
Duc, H. T., Rucay, P., Righenzi, S., Halle-Pannenko, O., Kourilsky, P., Malissen, B.
Monocional antibodies which recognize a synthetic peptide derived from influenza virus nucleoprotein in association with a murine class I MHC molecule (Kd) have been isolated. One such antibody has...
The role of the adenovirus E1a region in transformation and oncogenesis (1986)
Jochemsen, A.G., Bos, J.L., Bernards, R.A., Israel, A., Kourilsky, P., ...
Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family (1982)
Lalanne, J.L., Bregegere, F., Delarbre, C., Abastado, J.P., Gachelin, G., Kourilsky, P.
The complete nucleotide sequences of three cDNAs coding for the C-terminal part of mouse histocompatibility (H-2) antigens, and for the 3′ non coding regions of these clones have been determined....
Structure of C-terminal half of two H-2 antigens from cloned mRNA (1981)
Brégégère, F., Abastado, J. P., Kvist, S., Rast, L., Lalanne, J. L., Garoff, H., ...
cDNA clone coding for part of a mouse H-2d major histocompatibility antigen (1981)
Kvist, S., Brégégère, F., Rask, L., Cami, B., Garoff, H., Daniel, F., ...
DNA organisation in the chicken lysozyme gene region (1981)
Baldacci, P., Royal, A., Brégégère, F., Abastado, J.P., Cami, B., Daniel, F., ...
DNA sequences surrounding the lysozyme gene of the chicken have been cloned in several recombinants which define a region of 40 Kb. We have detected no other gene with a sequence related to that of...
Colbere-Garapin, F., Chousterman, S., Horodniceanu, F., Kourilsky, P., Garapin, A. C.
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned...
Colbere-Garapin, F., Chousterman, S., Horodniceanu, F., Kourilsky, P., Garapin, A. C.
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned...
Isolation of the lysozyme gene of chicken (1979)
Baldacci, P., Royal, A., Cami, B., Perrin, F., Krust, A., Garapin, A., ...
The lysozyme gene has been purified by molecular cloning from two chicken gene libraries. Several recombinant phages harbouring sequences homologous to a plasmid carrying a double stranded lysozyme...
The ovalbumin split gene: molecular cloning of Eco RI fragments "c" and "d" (1978)
LePennec, J.P., Baldacci, P., Perrin, F., Cami, B., Gerlinger, P., Krust, A., ...
The Eco RI fragments “c” and “d” of the ovalbumin gene (1, 2) have been isolated by molecular cloning. Restriction enzyme mapping and electron microscopy have confirmed that the two fragments...
Insertion of a rabbit {beta}-globin gene sequence into an E.coli plasmid (1975)
Rougeon, F., Kourilsky, P., Mach, B.
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E.coli plasmid was cleaved by EcoRI. The cohesive ends were repaired...
Apostolou, I., Takahama, Y., Belmant, C., Kawano, T., Huerre, M., Marchal, G., ...
Mice injected with deproteinized cell walls prepared from the strain H37rv of Mycobacterium tuberculosis develop a granuloma-like lesion in which NKT cells are predominant. NKT cells play a primary...
Musette, P, Bequet, D, Delarbre, C, Gachelin, G, Kourilsky, P, Dormont, D
We have used a PCR-based technology to study the V beta 5 and V beta 17 repertoire of T-cell populations in HLA-DR2 multiple sclerosis (MS) patients. We have found that the five MS DR2 patients...
Pannetier, C, Cochet, M, Darche, S, Casrouge, A, Zöller, M, Kourilsky, P
A method using PCR amplification and primer extension with fluorescent oligonucleotides was developed to analyze T-cell repertoires. The sizes of the hypervariable CDR3-like regions of the murine...
Duplication of the CD8 beta-chain gene as a marker of the man-gorilla-chimpanzee clade.
Delarbre, C, Nakauchi, H, Bontrop, R, Kourilsky, P, Gachelin, G
In earlier studies we have found that the gene encoding the CD8 beta chain is duplicated in man. We demonstrate here that the duplicated genes are both located on chromosome 2. We have also studied...
Cibotti, R, Kanellopoulos, J M, Cabaniols, J P, Halle-Panenko, O, Kosmatopoulos, K, Sercarz, E, ...
We have produced transgenic mice expression hen egg-white lysozyme (HEL) under the control of a ubiquitous promoter, so that in transgenic animals, HEL is presumably present in the serum and thymus...
Blanchet, O, Bourge, J F, Zinszner, H, Israel, A, Kourilsky, P, Dausset, J, ...
Class I antigens encoded in the major histocompatibility complex (MHC) (HLA in man, H-2 in the mouse) play a key role in the recognition of target cells by cytolytic T lymphocytes. Tumor cells...
Abastado, J P, Darche, S, Godeau, F, Cami, B, Kourilsky, P
We describe a system to analyze the individual contribution of a single physical DNA end on intramolecular recombination between partially homologous sequences. We took advantage of this partial...
Israël, A, Kimura, A, Kieran, M, Yano, O, Kanellopoulos, J, Le Bail, O, ...
Using gel retardation and in vitro "footprinting", we have analyzed the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of...
Working principles in the immune system implied by the "peptidic self" model.
Kourilsky, P, Chaouat, G, Rabourdin-Combe, C, Claverie, J M
The hypothesis that self as well as foreign proteins are processed into peptides and presented by major histocompatibility complex antigens leads to a set of working principles that could govern...
Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation.
Pannetier, C, Delassus, S, Darche, S, Saucier, C, Kourilsky, P
In vitro enzymatic amplification of nucleic acids by PCR or other techniques is a very sensitive method to detect rare DNA segments. We present here a protocol that allows the rapid, sensitive and...
Israel, A, Yano, O, Logeat, F, Kieran, M, Kourilsky, P
The MHC class I murine and beta-2-microglobulin genes are silent in embryonal carcinoma (EC) cells but are induced upon differentiation of these cells. We have previously shown that enhancer-like...
cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.
Kvist, S, Bregegere, F, Rask, L, Cami, B, Garoff, H, Daniel, F, ...
mRNA coding for mouse major transplantation antigens of the d haplotype was partially purified, copied into double-stranded cDNA, and cloned in Escherichia coli. Clones were selected by their ability...
Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli.
Garapin, A C, Colbère-Garapin, F, Cohen-Solal, M, Horodniceanu, F, Kourilsky, P
A herpes simplex virus type I DNA fragment containing the sequence coding for thymidine kinase was fused to the very beginning of the Escherichia coli lac Z gene in the three possible reading frames....
Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.
Lalanne, J L, Bregegere, F, Delarbre, C, Abastado, J P, Gachelin, G, Kourilsky, P
The complete nucleotide sequences of three cDNAs coding for the C-terminal part of mouse histocompatibility (H-2) antigens, and for the 3' non coding regions of these clones have been determined....
Vincent, C, Tchen, P, Cohen-Solal, M, Kourilsky, P
We have synthetised 8-(2-4 dinitrophenyl 2-6 aminohexyl) amino-adenosine 5' triphosphate (in short : rATP-DNP), a derivative of ATP which carries a dinitrophenyl group. We show that rATP-DNP is a...
DNA organisation in the chicken lysozyme gene region.
Baldacci, P, Royal, A, Brégégère, F, Abastado, J P, Cami, B, Daniel, F, ...
DNA sequences surrounding the lysozyme gene of the chicken have been cloned in several recombinants which define a region of 40 Kb. We have detected no other gene with a sequence related to that of...
Isolation of the lysozyme gene of chicken.
Baldacci, P, Royal, A, Cami, B, Perrin, F, Krust, A, Garapin, A, ...
The lysozyme gene has been purified by molecular cloning from two chicken gene libraries. Several recombinant phages harbouring sequences homologous to a plasmid carrying a double stranded lysozyme...
Godeau, F, Saucier, C, Kourilsky, P
The expression of the thymidine-thymidylate kinase (HSV1-TK), (ATP: thymidine 5'-phosphotransferase; EC 2.7.1.21) of herpes simplex virus type 1 endows the host cell with a conditional lethal...
Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.
We have developped in situ methods of colony hybridization in which there is no need to replicate colonies one by one prior to hybridization. The best method consists in promoting partial lysis of...
Humphries, P, Cochet, M, Krust, A, Gerlinger, P, Kourilsky, P, Chambon, P
Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The...
The ovalbumin split gene: molecular cloning of Eco RI fragments "c" and "d".
LePennec, J P, Baldacci, P, Perrin, F, Cami, B, Gerlinger, P, Krust, A, ...
The Eco RI fragments "c" and "d" of the ovalbumin gene (1, 2) have been isolated by molecular cloning. Restriction enzyme mapping and electron microscopy have confirmed that the two fragments contain...
Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
Rougeon, F, Kourilsky, P, Mach, B
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired...
Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.
Cami, B, Chambon, P, Kourilsky, P
We have prepared heteroduplexes between two plasmids that carry, in the same orientation, two H-2 cDNA inserts, 1.15 and 1.0 kilobase long, respectively. Their sequences encode two distinct class I...
Molecular cloning of the genome of poliovirus type 1.
Bregegere, F, Kopecka, H, Kitamura, N, Rothberg, P G, Kourilsky, P, ...
Poliovirus cDNA.RNA hybrids were prepared from the Mahoney strain of poliovirus type 1 by using reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) and cloned in the Escherichia coli...
Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
Colbere-Garapin, F, Chousterman, S, Horodniceanu, F, Kourilsky, P, Garapin, A C
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned...
Processing of complex heteroduplexes in Escherichia coli and Cos-1 monkey cells.
Abastado, J P, Cami, B, Dinh, T H, Igolen, J, Kourilsky, P
Upon transformation into Escherichia coli or Cos-1 monkey cells, heteroduplex DNA made of two sequences containing many nucleotide mismatches yields a wide array of different molecules, some with a...
Israël, A, Le Bail, O, Hatat, D, Piette, J, Kieran, M, Logeat, F, ...
We have dissected the mouse H-2Kb gene promoter in order to define the sequences responsible for induction by tumour necrosis factor (TNF-alpha). An enhancer element (-187 to -158) composed of two...
Methods for identification of recombinants of phage lambda.
Sanzey, B, Mercereau, O, Ternynck, T, Kourilsky, P
Two methods are described which allow the screening of a large number of phage plaques for a specific DNA sequence carried by the phage or a specific antigen produced within the phage plaque. These...
Logeat, F, Israël, N, Ten, R, Blank, V, Le Bail, O, Kourilsky, P, ...
The KBF1 factor, which binds to the enhancer A located in the promoter of the mouse MHC class I gene H-2Kb, is indistinguishable from the p50 DNA binding subunit of the transcription factor NF-kappa...
Blank, V, Kourilsky, P, Israël, A
The transcription factor NF-kappa B binds to DNA as a heterodimer composed of two subunits of 50 kDa (p50) and 65 kDa (p65). p50 contains a DNA binding and dimerization domain and represents a...
Lalanne, J L, Cochet, M, Kummer, A M, Gachelin, G, Kourilsky, P
A cDNA library constructed from liver mRNA of DBA/2 (H-2d) mice has been screened with H-2-specific probes. The nucleotide sequence of one clone (pH-2d-24) indicates that it derives from an H-2 gene...
Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers.
Yano, O, Kanellopoulos, J, Kieran, M, Le Bail, O, Israël, A, Kourilsky, P
An enhancer binding factor, designated KBF1, has been purified from the nuclear extract of mouse BW5147 thymoma cells by five column chromatography steps including a sequence-specific DNA affinity...
Late transient expression of human hepatitis B virus genes in monkey cells.
Colbère-Garapin, F, Horodniceanu, F, Kourilsky, P, Garapin, A C
The expression of human hepatitis B virus (HBV) surface (HBS) and e (HBe) antigens has been studied comparatively in monkey and mouse cell lines co-transfected with HBV DNA and the dominant selective...
Daniel, F, Morello, D, Le Bail, O, Chambon, P, Cayre, Y, Kourilsky, P
Mouse teratocarcinoma cells express neither H-2 heavy chains nor beta 2-microglobulin (beta 2-m). We have constructed two genomic libraries, one from PCC4-aza-RI embryonal carcinoma cells and the...
Ten, R M, Paya, C V, Israël, N, Le Bail, O, Mattei, M G, Virelizier, J L, ...
In order to characterize the regulation of the gene encoding the p50 subunit of the transcription factor NF-kappa B, we have isolated a human genomic clone, and sequenced the promoter of this gene....
Functional expression of a mouse H-2Kb gene isolated from non-expressing teratocarcinoma cells.
Daniel-Vedele, F, Morello, D, Benicourt, C, Transy, C, Le Bail, O, Plata, F, ...
Embryonal carcinoma cells do not express H-2 antigens or beta 2-microglobulin. Recent studies have suggested that the expression of these antigens is likely to be controlled at the level of...
Alternative splicing in the 5' moiety of the H-2Kd gene transcript.
Transy, C, Lalanne, J L, Kourilsky, P
The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They...
Adenovirus E1A-mediated regulation of class I MHC expression.
Vaessen, R T, Houweling, A, Israel, A, Kourilsky, P, Van Der Eb, A J
Expression of class I MHC transplantation antigens has been shown to be reduced in baby rat kidney (BRK) cells transformed by highly oncogenic adenovirus type 12 (Ad12), as compared with...
Apostolou, I., Takahama, Y., Belmant, C., Kawano, T., Huerre, M., Marchal, G., ...
Mice injected with deproteinized cell walls prepared from the strain H37rv of Mycobacterium tuberculosis develop a granuloma-like lesion in which NKT cells are predominant. NKT cells play a primary...
Musette, P, Bequet, D, Delarbre, C, Gachelin, G, Kourilsky, P, Dormont, D
We have used a PCR-based technology to study the V beta 5 and V beta 17 repertoire of T-cell populations in HLA-DR2 multiple sclerosis (MS) patients. We have found that the five MS DR2 patients...
Pannetier, C, Cochet, M, Darche, S, Casrouge, A, Zöller, M, Kourilsky, P
A method using PCR amplification and primer extension with fluorescent oligonucleotides was developed to analyze T-cell repertoires. The sizes of the hypervariable CDR3-like regions of the murine...
Duplication of the CD8 beta-chain gene as a marker of the man-gorilla-chimpanzee clade.
Delarbre, C, Nakauchi, H, Bontrop, R, Kourilsky, P, Gachelin, G
In earlier studies we have found that the gene encoding the CD8 beta chain is duplicated in man. We demonstrate here that the duplicated genes are both located on chromosome 2. We have also studied...
Cibotti, R, Kanellopoulos, J M, Cabaniols, J P, Halle-Panenko, O, Kosmatopoulos, K, Sercarz, E, ...
We have produced transgenic mice expression hen egg-white lysozyme (HEL) under the control of a ubiquitous promoter, so that in transgenic animals, HEL is presumably present in the serum and thymus...
Blanchet, O, Bourge, J F, Zinszner, H, Israel, A, Kourilsky, P, Dausset, J, ...
Class I antigens encoded in the major histocompatibility complex (MHC) (HLA in man, H-2 in the mouse) play a key role in the recognition of target cells by cytolytic T lymphocytes. Tumor cells...
Abastado, J P, Darche, S, Godeau, F, Cami, B, Kourilsky, P
We describe a system to analyze the individual contribution of a single physical DNA end on intramolecular recombination between partially homologous sequences. We took advantage of this partial...
Israël, A, Kimura, A, Kieran, M, Yano, O, Kanellopoulos, J, Le Bail, O, ...
Using gel retardation and in vitro "footprinting", we have analyzed the interactions between nuclear proteins derived from various mouse cells and the enhancer and interferon response sequences of...
Working principles in the immune system implied by the "peptidic self" model.
Kourilsky, P, Chaouat, G, Rabourdin-Combe, C, Claverie, J M
The hypothesis that self as well as foreign proteins are processed into peptides and presented by major histocompatibility complex antigens leads to a set of working principles that could govern...
Quantitative titration of nucleic acids by enzymatic amplification reactions run to saturation.
Pannetier, C, Delassus, S, Darche, S, Saucier, C, Kourilsky, P
In vitro enzymatic amplification of nucleic acids by PCR or other techniques is a very sensitive method to detect rare DNA segments. We present here a protocol that allows the rapid, sensitive and...
Israel, A, Yano, O, Logeat, F, Kieran, M, Kourilsky, P
The MHC class I murine and beta-2-microglobulin genes are silent in embryonal carcinoma (EC) cells but are induced upon differentiation of these cells. We have previously shown that enhancer-like...
cDNA clone coding for part of a mouse H-2d major histocompatibility antigen.
Kvist, S, Bregegere, F, Rask, L, Cami, B, Garoff, H, Daniel, F, ...
mRNA coding for mouse major transplantation antigens of the d haplotype was partially purified, copied into double-stranded cDNA, and cloned in Escherichia coli. Clones were selected by their ability...
Expression of herpes simplex virus type I thymidine kinase gene in Escherichia coli.
Garapin, A C, Colbère-Garapin, F, Cohen-Solal, M, Horodniceanu, F, Kourilsky, P
A herpes simplex virus type I DNA fragment containing the sequence coding for thymidine kinase was fused to the very beginning of the Escherichia coli lac Z gene in the three possible reading frames....
Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.
Lalanne, J L, Bregegere, F, Delarbre, C, Abastado, J P, Gachelin, G, Kourilsky, P
The complete nucleotide sequences of three cDNAs coding for the C-terminal part of mouse histocompatibility (H-2) antigens, and for the 3' non coding regions of these clones have been determined....
Vincent, C, Tchen, P, Cohen-Solal, M, Kourilsky, P
We have synthetised 8-(2-4 dinitrophenyl 2-6 aminohexyl) amino-adenosine 5' triphosphate (in short : rATP-DNP), a derivative of ATP which carries a dinitrophenyl group. We show that rATP-DNP is a...
DNA organisation in the chicken lysozyme gene region.
Baldacci, P, Royal, A, Brégégère, F, Abastado, J P, Cami, B, Daniel, F, ...
DNA sequences surrounding the lysozyme gene of the chicken have been cloned in several recombinants which define a region of 40 Kb. We have detected no other gene with a sequence related to that of...
Isolation of the lysozyme gene of chicken.
Baldacci, P, Royal, A, Cami, B, Perrin, F, Krust, A, Garapin, A, ...
The lysozyme gene has been purified by molecular cloning from two chicken gene libraries. Several recombinant phages harbouring sequences homologous to a plasmid carrying a double stranded lysozyme...
Godeau, F, Saucier, C, Kourilsky, P
The expression of the thymidine-thymidylate kinase (HSV1-TK), (ATP: thymidine 5'-phosphotransferase; EC 2.7.1.21) of herpes simplex virus type 1 endows the host cell with a conditional lethal...
Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.
We have developped in situ methods of colony hybridization in which there is no need to replicate colonies one by one prior to hybridization. The best method consists in promoting partial lysis of...
Humphries, P, Cochet, M, Krust, A, Gerlinger, P, Kourilsky, P, Chambon, P
Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The...
The ovalbumin split gene: molecular cloning of Eco RI fragments "c" and "d".
LePennec, J P, Baldacci, P, Perrin, F, Cami, B, Gerlinger, P, Krust, A, ...
The Eco RI fragments "c" and "d" of the ovalbumin gene (1, 2) have been isolated by molecular cloning. Restriction enzyme mapping and electron microscopy have confirmed that the two fragments contain...
Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
Rougeon, F, Kourilsky, P, Mach, B
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired...
Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.
Cami, B, Chambon, P, Kourilsky, P
We have prepared heteroduplexes between two plasmids that carry, in the same orientation, two H-2 cDNA inserts, 1.15 and 1.0 kilobase long, respectively. Their sequences encode two distinct class I...
Molecular cloning of the genome of poliovirus type 1.
Bregegere, F, Kopecka, H, Kitamura, N, Rothberg, P G, Kourilsky, P, ...
Poliovirus cDNA.RNA hybrids were prepared from the Mahoney strain of poliovirus type 1 by using reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) and cloned in the Escherichia coli...
Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
Colbere-Garapin, F, Chousterman, S, Horodniceanu, F, Kourilsky, P, Garapin, A C
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned...
Processing of complex heteroduplexes in Escherichia coli and Cos-1 monkey cells.
Abastado, J P, Cami, B, Dinh, T H, Igolen, J, Kourilsky, P
Upon transformation into Escherichia coli or Cos-1 monkey cells, heteroduplex DNA made of two sequences containing many nucleotide mismatches yields a wide array of different molecules, some with a...
Israël, A, Le Bail, O, Hatat, D, Piette, J, Kieran, M, Logeat, F, ...
We have dissected the mouse H-2Kb gene promoter in order to define the sequences responsible for induction by tumour necrosis factor (TNF-alpha). An enhancer element (-187 to -158) composed of two...
Methods for identification of recombinants of phage lambda.
Sanzey, B, Mercereau, O, Ternynck, T, Kourilsky, P
Two methods are described which allow the screening of a large number of phage plaques for a specific DNA sequence carried by the phage or a specific antigen produced within the phage plaque. These...
Logeat, F, Israël, N, Ten, R, Blank, V, Le Bail, O, Kourilsky, P, ...
The KBF1 factor, which binds to the enhancer A located in the promoter of the mouse MHC class I gene H-2Kb, is indistinguishable from the p50 DNA binding subunit of the transcription factor NF-kappa...
Blank, V, Kourilsky, P, Israël, A
The transcription factor NF-kappa B binds to DNA as a heterodimer composed of two subunits of 50 kDa (p50) and 65 kDa (p65). p50 contains a DNA binding and dimerization domain and represents a...
Lalanne, J L, Cochet, M, Kummer, A M, Gachelin, G, Kourilsky, P
A cDNA library constructed from liver mRNA of DBA/2 (H-2d) mice has been screened with H-2-specific probes. The nucleotide sequence of one clone (pH-2d-24) indicates that it derives from an H-2 gene...
Purification of KBF1, a common factor binding to both H-2 and beta 2-microglobulin enhancers.
Yano, O, Kanellopoulos, J, Kieran, M, Le Bail, O, Israël, A, Kourilsky, P
An enhancer binding factor, designated KBF1, has been purified from the nuclear extract of mouse BW5147 thymoma cells by five column chromatography steps including a sequence-specific DNA affinity...
Late transient expression of human hepatitis B virus genes in monkey cells.
Colbère-Garapin, F, Horodniceanu, F, Kourilsky, P, Garapin, A C
The expression of human hepatitis B virus (HBV) surface (HBS) and e (HBe) antigens has been studied comparatively in monkey and mouse cell lines co-transfected with HBV DNA and the dominant selective...
Daniel, F, Morello, D, Le Bail, O, Chambon, P, Cayre, Y, Kourilsky, P
Mouse teratocarcinoma cells express neither H-2 heavy chains nor beta 2-microglobulin (beta 2-m). We have constructed two genomic libraries, one from PCC4-aza-RI embryonal carcinoma cells and the...
Ten, R M, Paya, C V, Israël, N, Le Bail, O, Mattei, M G, Virelizier, J L, ...
In order to characterize the regulation of the gene encoding the p50 subunit of the transcription factor NF-kappa B, we have isolated a human genomic clone, and sequenced the promoter of this gene....
Functional expression of a mouse H-2Kb gene isolated from non-expressing teratocarcinoma cells.
Daniel-Vedele, F, Morello, D, Benicourt, C, Transy, C, Le Bail, O, Plata, F, ...
Embryonal carcinoma cells do not express H-2 antigens or beta 2-microglobulin. Recent studies have suggested that the expression of these antigens is likely to be controlled at the level of...
Alternative splicing in the 5' moiety of the H-2Kd gene transcript.
Transy, C, Lalanne, J L, Kourilsky, P
The H-2Kd gene, which encodes a mouse major transplantation antigen, was transfected into L TK- mouse fibroblasts. Two transcripts of the gene were detected by S1 nuclease mapping analysis. They...
Adenovirus E1A-mediated regulation of class I MHC expression.
Vaessen, R T, Houweling, A, Israel, A, Kourilsky, P, Van Der Eb, A J
Expression of class I MHC transplantation antigens has been shown to be reduced in baby rat kidney (BRK) cells transformed by highly oncogenic adenovirus type 12 (Ad12), as compared with...