P. Schwille

Importin 8 Is a Gene Silencing Factor that Targets Argonaute Proteins to Distinct mRNAs (2009)

Weinmann, L., Höck, J., Ivacevic, T., Ohrt, T., Mutze, J., Schwille, P., ...

Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs. (2009)

Weinmann, L., Hoeck, J., Ivacevic, T., Ohrt, T., Muetze, J., Schwille, P., ...

Ceramide triggers budding of exosome vesicles into multivesicular endosomes. (2008)

Trajkovic, K., Hsu, C., Chiantia, S., Rajendran, L., Wenzel, D., Wieland, F., ...

Teaching nanoscience across scientific and geographical borders ? A European Master programme in nanoscience and nanotechnology (2008)

Chesneau, A., Groeseneken, G., Heremans, P., Rep, D., Rudquist, P., Schwille, P., ...

Accumulation and filtering of nanoparticles in microchannels using electrohydrodynamically induced vortical flows (2008)

Felten, M., Staroske, W., Jaeger, M.S., Schwille, P., Duschl, C.

We present an approach for the accumulation and filtering of nano- and microparticles in microfluidic devices that is based on the generation of electric traveling waves in the radio-frequency range....

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells (2008)

Ohrt, T., Muetze, J., Staroske, W., Weinmann, L., Höck, J., Crell, K., ...

Teaching nanoscience across scientific and geographical borders – A European Master programme in nanoscience and nanotechnology (2008)

Chesneau, A., Groeseneken, G., Heremans, P., Rep, D., Rudquist, P., Schwille, P., ...

Spatial regulators for bacterial cell division self-organize into surface waves in vitro (2008)

Loose, M., Fischer-Friedrich, E., Ries, J., Kruse, K., Schwille, P.

Teaching nanoscience across scientific and geographical borders – A European Master programme in nanoscience and nanotechnology (2008)

Chesneau, A, Groeseneken, G, Heremans, P, Rep, D, Rudquist, P, Schwille, P, ...

Within the Erasmus Mundus Master (EMM) Programme, five European Universities (KU Leuven, Belgium, Chalmers University of Technology, Sweden, Delft University of Technology and Leiden University, the...

Immune complexes of auto-antibodies against Aβ1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients (2007)

A W Henkel, P S Dittrich, T W Groemer, E A Lemke, J Klingauf, H W Klafki, ...

Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy (2007)

Meacci, G., Ries, J., Fischer-Friedrich, E., Kahya, N., Schwille, P., Kruse, K.

In the bacterium Escherichia coli, selection of the division site involves pole-to-pole oscillations of the proteins MinD and MinE. Different oscillation mechanisms based on cooperative effects...

Immune complexes of auto-antibodies against A beta 1-42 peptides patrol cerebrospinal fluid of non-Alzheimer's patients (2007)

Henkel, A. W., Dittrich, P. S., Groemer, T., Lemke, E. A., Klingauf, J., Klafki, H. W., ...

Phosphatidylethanolamine critically supports internalization of cell-penetrating protein C inhibitor (2007)

Baumgaertner, P., Geiger, M., Zieseniss, S., Malleier, J., Huntington, J. A., Hochrainer, K., ...

The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology (2006)

Gast, F.-U., Dittrich, P.S., Schwille, P., Weigel, M., Mertig, M., Opitz, J., ...

Fluorescence lifetime images and correlation spectra obtained by multidimensional time-correlated single photon counting (2006)

Becker, W., Bergmann, A., Haustein, E., Petrasek, Z., Schwille, P., Biskup, C., ...

Mobility of Min-proteins in Escherichia coli measured by fluorescence correlation spectroscopy (2006)

Meacci, G., Ries, J., Fischer-Friedrich, E., Kahya, N., Schwille, P., Kruse, K.

The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology (2006)

Gast, F U, Dittrich, P S, Schwille, P, Weigel, M, Mertig, M, Opitz, J, ...

We describe a novel microfluidic perfusion system for high-resolution microscopes. Its modular design allows pre-coating of the coverslip surface with reagents, biomolecules, or cells. A...

Fluorescence lifetime images and correlation spectra obtained by multidimensional TCSPC (2005)

Becker, W., Bergmann, A., Haustein, E., Petrasek, Z., Schwille, P., Biskup, C., ...

Characterization of interaction between cationic lipid-oligonucleotide complexes and cellular membrane lipids using confocal imaging and fluorescence correlation spectroscopy (2005)

Gordon, S. P., Berezhna, S., Scherfeld, D., Kahya, N., Schwille, P.

Breakdown of axonal synaptic vesicle precursor transport by microglial nitric oxide (2005)

Stagi, M., Dittrich, P. S., Frank, N., Iliev, A. I., Schwille, P., Neumann, H.

New effects in polynucleotide release from cationic lipid carriers revealed by confocal imaging, fluorescence cross-correlation spectroscopy and single particle tracking (2005)

Berezhna, S., Schaefer, S., Heintzmann, R., Jahnz, M., Boese, G., Deniz, A., ...

We report on new insights into the mechanisms of short single and double stranded oligonucleotide release from cationic lipid complexes (lipoplexes), used in gene therapy. Specifically, we modeled...

Characterization of the photoconversion on reaction of the fluorescent protein kaede on the single-molecule level (2005)

Dittrich, P. S., Schaefer, S., Schwille, P.

Breakdown of axonal synaptic vesicle precursor transport by microglial nitric oxide (2005)

Stagi, M., Dittrich, P. S., Frank, N., Iliev, A. I., Schwille, P., Neumann, H.

Structural changes and binding in UvrABC monitored by single-molecule fluorescence (2005)

Haustein, E., Boese, G., Merkle, D., Schwille, P.

Differential lipid packing abilities and dynamics in giant unilamellar vesicles composed of short-chain saturated glycerol-phospholipids, sphingomyelin and cholesterol (2005)

Kahya, N., Scherfeld, D., Schwille, P.

Fluorescence correlation spectroscopy with autofluorescent proteins (2005)

Kohl, T., Schwille, P.

Lipids as modulators of proteolytic activity of BACE - Involvement of cholesterol, glycosphingolipids, and anionic phospholipids in vitro (2005)

Kalvodova, L., Kahya, N., Schwille, P., Ehehalt, R., Verkade, P., Drechsel, D., ...

Raft partitioning and dynamic behavior of human placental alkaline phosphatase in giant unilamellar vesicles (2005)

Kahya, N., Brown, D. A., Schwille, P.

Raft-targeting and lateral dynamic behavior of membrane proteins in Giant Unilamellar Vesicles (GUVs) (2005)

Kahya, N., Kalvodova, L., Bacia, K., Brown, D. A., Simons, K., Schwille, P.

Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes (2005)

Bacia, K., Schwille, P., Kurzchalia, T.

Intracellular calmodulin availability accessed with two-photon cross-correlation (2004)

Kim, S. A., Heinze, K. G., Waxham, M. N., Schwille, P.

Fluorescence correlation spectroscopy relates rafts in model and native membranes (2004)

Bacia, K., Scherfeld, D., Kahya, N., Schwille, P.

Studying SNARE proteins incorporated into giant unilamellar vesicles by confocal imaging and fluorescence (cross-)correlation spectroscopy (2004)

Bacia, K., Schuette, C. G., Kahya, N., Scherfeld, D., Jahn, R., Schwille, P.

Towards single molecule detection in living cells: Single molecule FRET of the repair Enzym UvrB in vitro and a transfection method for fluorescently labeled biomolecules (2004)

Boese, G., Haustein, E., Schwille, P.

The mobility of phytochrome within protonemal tip cells of the moss Ceratodon purpureus, monitored by fluorescence correlation spectroscopy (2004)

Boese, G., Schwille, P., Lamparter, T.

Studying reaction kinetics by simultaneous FRET and cross-correlation analysis in a miniaturized continuous flow reactor (2004)

Dittrich, P. S., Mueller, B., Schwille, P.

More information from triple-labeled biomolecules? (2004)

Haustein, E., Boese, G., Jahnz, M., Schwille, P.

Enzyme assays for confocal single molecule spectroscopy (2004)

Jahnz, M., Schwille, P.

Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy (2004)

Kahya, N., Scherfeld, D., Bacia, K., Schwille, P.

Single-molecule spectroscopic methods (2004)

Haustein, E., Schwille, P.

SNAREs prefer liquid-disordered over "raft" (liquid-ordered) domains when reconstituted into giant unilamellar vesicles (2004)

Bacia, K., Schuette, C. G., Kahya, N., Jahn, R., Schwille, P.

Single molecule FCS-based oxygen sensor (O2-FCSensor): a new intrinsically calibrated oxygen sensor utilizing fluorescence correlation spectroscopy (FCS) with single fluorescent molecule detection sensitivity (2003)

Opitz,Norbert, Rothwell,Paul James, Oeke,Brigitte, Schwille,P.

A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy (2003)

Bacia, K., Schwille, P.

Fluorescence correlation spectroscopy (FCS) is becoming increasingly popular as a technique that aims at complementing live cell images with biophysical information. This article provides both a...

Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy (2003)

Haustein, E., Schwille, P.

Fluorescence correlation spectroscopy (FCS) extracts information about molecular dynamics from the tiny fluctuations that can be observed in the emission of small ensembles of fluorescent molecules...

Single molecule FCS-based oxygen sensor (O2-FCSensor): a new intrinsically calibrated oxygen sensor utilizing fluorescence correlation spectroscopy (FCS) with single fluorescent molecule detection sensitivity (2003)

Opitz, Norbert, Rothwell, Paul James, Oeke, Brigitte, Schwille, P.

An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles (2003)

Dittrich, P. S., Schwille, P.

Intracellular applications of fluorescence correlation spectroscopy: prospects for neuroscience (2003)

Kim, S. A., Schwille, P.

Lipid dynamics and domain formation in model membranes composed of ternary mixtures of unsaturated and saturated phosphatidylcholines and cholesterol (2003)

Scherfeld, D., Kahya, N., Schwille, P.

Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopy (2003)

Kahya, N., Scherfeld, D., Bacia, K., Poolman, B., Schwille, P.

Triple FRET: A tool for studying long-range molecular interactions (2003)

Haustein, E., Jahnz, M., Schwille, P.

Lateral diffusion of membrane lipid-anchored probes before and after aggregation of cell surface IgE-receptors (2003)

Pyenta, P. S., Schwille, P., Webb, W. W., Holowka, D., Baird, B.

Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis (2002)

Bacia, K., Majoul, I., Schwille, P.

Fluorescence (auto)correlaion spectroscopy (FCS) has developed into a widely used method for investigating molecular dynamics and mobility of molecules in vitro and in vivo. Dual-color...

Fluorescence correlation spectroscopy for the detection and study of single molecules in Biology (2002)

Medina, M. A., Schwille, P.

The recent development of single molecule detection techniques has opened new horizons for the study of individual macromolecules under physiological conditions. Conformational subpopulations,...

Two-photon fluorescence coincidence analysis: Rapid measurements of enzyme kinetics (2002)

Heinze, K. G., Rarbach, M., Jahnz, M., Schwille, P.

Dual-color fluorescence cross-correlation analysis is a powerful tool for probing interactions of different fluorescently labeled molecules in aqueous solution. The concept is the selective...

Spatial two-photon fluorescence cross-correlation Spectroscopy for controlling molecular transport in microfluidic structures (2002)

Dittrich, P. S., Schwille, P.

The increasing availability of microfluidic systems of various geometries and materials for the downscaling of chemical or biochemical processes raises a strong demand for adequate techniques to...

A protease assay for two-photon crosscorrelation and FRET analysis based solely on fluorescent proteins (2002)

Kohl, T., Heinze, K. G., Kuhlemann, R., Koltermann, A., Schwille, P.

GFP and the red fluorescent protein, DsRed, have been combined to design a protease assay that allows not only for fluorescence resonance energy transfer (FRET) studies but also for dual-color...

Scanning dual-color cross-correlation analysis for dynamic co- localization studies of immobile molecules (2002)

Amediek, A., Haustein, E., Scherfeld, D., Schwille, P.

Dual-color fluorescence cross-correlation analysis has proven to be a powerful tool to probe interactions of different molecular species in solution and living cells on a single molecule level....

Complex formation of Golgi resident proteins in vivo as revealed by fluorescence correlation spectroscopy (2002)

Weiss, M., Schwille, P., Storrie, B., Nilsson, T.

FLP recombinase: Changing substrate specificity by evolutionary biotechnology (2002)

Jahnz, M., Bose, G., Koltermann, A., Schwille, P.

Intracellular measurements of calmodulin and CaMKII interactions using multiphoton fluorescence cross-correlation spectroscopy (MPFCCS) (2002)

Kim, S. A., Heinze, K. G., Waxham, M. N., Schwille, P.

Monitoring protease activity on a single molecule scale using Two-Photon Cross-Correlation and FRET analysis (2002)

Heinze, K. G., Kohl, T., Kuhlemann, R., Schwille, P.

Caging single molecules (2002)

Haustein, E., Scherfeld, D., Schwille, P.

A new detection system for genotoxic substances using excinuclease UvrAB with fluorescence correlation spectroscopy (2002)

Boese, G., Schwille, P.

Photobleaching and stabilization of fluorophores used for single-molecule analysis with one- and two-photon excitation (2001)

Dittrich, P. S., Schwille, P.

Fluorescence spectroscopic measurements at the single-molecule level usually require large absorption cross sections and fluorescence quantum yields for the dyes under study. In addition to these...

Accessing Molecular Dynamics in Cells by Fluorescence Correlation Spectroscopy. (2001)

Dittrich, P. S., Malvezzi-Campeggi, F., Jahnz, M., Schwille, P.

Cross-correlation analysis in FCS. (2001)

Elson, E. L., Rigler, R., Schwille, P.

Ultrasensitive Probing of Molecular Microenvironment by Fluorescence Correlation Spectroscopy. (2001)

Hein, H. J., Bischoff, G., Schwille, P.

Photophysical investigations correlation spectroscopy provide evidence of different bright states in DsRed. (2001)

Malvezzi-Campeggi, F., Jahnz, M., Heinze, K. G., Dittrich, P. S., Schwille, P.

Fluorescence correlation spectroscopy and its potential for intracellular applications. (2001)

Schwille, P.

Two-photon fluorescence cross-correlation spectroscopy. (2001)

Schwille, P., Heinze, K. G.

Analyzing Single Protein Molecules by Optical Methods. (2001)

Schwille, P., Kettling, U.

Simultaneous Two-Photon Excitation of Distinct Labels For Dual-Color Fluorescence Cross-Correlation Analysis. (2000)

Heinze, K. G., Koltermann, A., Schwille, P.

Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins. (2000)

Schwille, P., Kummer, S., Heikal, A. A., Moerner, W. E., Webb, W. W.

Characterization of photo-induced isomerization and back-isomerization of the cyanine dye Cy5 by fluorescence correlation spectroscopy. (2000)

Widengren, J., Schwille, P.

Characterization of lipid bilayer phases by confocal microscopy and fluorescence correlation spectroscopy. (1999)

Korlach, J., Schwille, P., Webb, W. W., Feigenson, G. F.

Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. (1999)

Schwille, P., Haupts, U., Maiti, S., Webb, W. W.

Fluorescence correlation spectroscopy with single molecule sensitivity on cell and model membranes. (1999)

Schwille, P., Korlach, J., Webb, W. W.

Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. (1998)

Kettling, U., Koltermann, A., Schwille, P., Eigen, M.

Kinetic investigations by fluorescence correlation spectroscopy: The analytical and diagnostic potential of diffusion studies (1997)

Schwille, P., Bieschke, J., Oehlenschlaeger, F.

Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution (1997)

Schwille, P., MeyerAlmes, F. J., Rigler, R.

Photophysical characterization of the dye Cy-5 by fluorescence correlation spectroscopy (1997)

Widengren, J., Schwille, P., Rigler, R.

Detection of HIV-1 RNA by nucleic acid sequence-based amplification combined with fluorescence correlation spectroscopy (1996)

Oehlenschlaeger, F., Schwille, P., Eigen, M.

Quantitative hybridization kinetics of DNA probes to RNA in solution followed by diffusional fluorescence correlation analysis (1996)

Schwille, P., Oehlenschlaeger, F., Walter, N.

Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution.

Schwille, P, Meyer-Almes, F J, Rigler, R

The present paper describes a new experimental scheme for following diffusion and chemical reaction systems of fluorescently labeled molecules in the nanomolar concentration range by fluorescence...

Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation.

Schwille, P, Haupts, U, Maiti, S, Webb, W W

Multiphoton excitation (MPE) of fluorescent probes has become an attractive alternative in biological applications of laser scanning microscopy because many problems encountered in spectroscopic...

Light-induced flickering of DsRed provides evidence for distinct and interconvertible fluorescent states.

Malvezzi-Campeggi, F, Jahnz, M, Heinze, K G, Dittrich, P, Schwille, P

Green fluorescent protein (GFP) from jellyfish Aequorea victoria, the powerful genetically encoded tag presently available in a variety of mutants featuring blue to yellow emission, has found a...

Characterization of the Photoconversion on Reaction of the Fluorescent Protein Kaede on the Single-Molecule Level

Dittrich, P. S., Schäfer, S. P., Schwille, P.

Fluorescent proteins are now widely used in fluorescence microscopy as genetic tags to any protein of interest. Recently, a new fluorescent protein, Kaede, was introduced, which exhibits an...

Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution.

Schwille, P, Meyer-Almes, F J, Rigler, R

The present paper describes a new experimental scheme for following diffusion and chemical reaction systems of fluorescently labeled molecules in the nanomolar concentration range by fluorescence...

Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation.

Schwille, P, Haupts, U, Maiti, S, Webb, W W

Multiphoton excitation (MPE) of fluorescent probes has become an attractive alternative in biological applications of laser scanning microscopy because many problems encountered in spectroscopic...

Light-induced flickering of DsRed provides evidence for distinct and interconvertible fluorescent states.

Malvezzi-Campeggi, F, Jahnz, M, Heinze, K G, Dittrich, P, Schwille, P

Green fluorescent protein (GFP) from jellyfish Aequorea victoria, the powerful genetically encoded tag presently available in a variety of mutants featuring blue to yellow emission, has found a...

Characterization of the Photoconversion on Reaction of the Fluorescent Protein Kaede on the Single-Molecule Level

Dittrich, P. S., Schäfer, S. P., Schwille, P.

Fluorescent proteins are now widely used in fluorescence microscopy as genetic tags to any protein of interest. Recently, a new fluorescent protein, Kaede, was introduced, which exhibits an...