Patrik Rorsman

Uromodulin mutations causing familial juvenile hyperuricaemic nephropathy lead to protein maturation defects and retention in the endoplasmic reticulum (2009)

Williams, Siân E., Reed, Anita A.C., Galvanovskis, Juris, Antignac, Corinne, Goodship, Tim, Karet, Fiona E., ...

Familial juvenile hyperuricaemic nephropathy (FJHN), an autosomal dominant disorder, is caused by mutations in the UMOD gene, which encodes Uromodulin, a glycosylphosphatidylinositol-anchored protein...

Quantification of mRNA in single cells and modelling of RT-qPCR induced noise (2008)

Bengtsson, Martin, Hemberg, Martin, Rorsman, Patrik, Ståhlberg, Anders

Abstract Background Gene expression has a strong stochastic element resulting in highly variable mRNA levels between individual cells, even in a seemingly homogeneous cell population. Access to...

CAPS1 and CAPS2 regulate stability and recruitment of insulin granules in mouse pancreatic β cells (2008)

Speidel, Dina, Salehi, Albert, Obermueller, Stefanie, Lundquist, Ingmar, Brose, Nils, Renström, Erik, ...

pVHL is a regulator of glucose metabolism and insulin secretion in pancreatic {beta} cells (2008)

Zehetner, Jens, Danzer, Carsten, Collins, Stephan, Eckhardt, Katrin, Gerber, Philipp A., Ballschmieter, Pia, ...

Insulin secretion from pancreatic β cells is stimulated by glucose metabolism. However, the relative importance of metabolizing glucose via mitochondrial oxidative phosphorylation versus glycolysis...

A KATP Channel-Dependent Pathway within α Cells Regulates Glucagon Release from Both Rodent and Human Islets of Langerhans (2007)

Patrick E. MacDonald, Reshma Ramracheya, Albert Salehi, Xiaosong Ma, ...

Glucagon, secreted from pancreatic islet α cells, stimulates gluconeogenesis and liver glycogen breakdown. The mechanism regulating glucagon release is debated, and variously attributed to neuronal...

R-type Ca(2+)-channel-evoked CICR regulates glucose-induced somatostatin secretion. (2007)

Zhang, Quan, Bengtsson, Martin, Partridge, Chris, Salehi, Albert, Braun, Matthias, Cox, Roger, ...

Abstract is not available

Long-term exposure to glucose and lipids inhibits glucose-induced insulin secretion downstream of granule fusion with plasma membrane. (2007)

Olofsson, Charlotta S, Collins, Stephan, Bengtsson, Martin, Eliasson, Lena, Salehi, Albert, Shimomura, Kenju, ...

Abstract is not available

Oscillations, Intercellular Coupling, and Insulin Secretion in Pancreatic β Cells (2006)

Patrick E. MacDonald, Patrik Rorsman

Insulin is a potent metabolic regulator that is released by pancreatic beta-cells, which respond to body glucose concentrations. Here the authors explain the physiological basis of insulin release.

Oscillations, Intercellular Coupling, and Insulin Secretion in Pancreatic β Cells (2006)

Patrick E. MacDonald, Patrik Rorsman

Failure of Transplanted Bone Marrow Cells to Adopt a Pancreatic {beta}-Cell Fate. (2006)

Taneera, Jalal, Rosengren, Anders, Renstrom, Erik, Nygren, Jens M, Serup, Palle, Rorsman, Patrik, ...

Abstract is not available

Antibody inhibition of synaptosomal protein of 25 kDa (SNAP-25) and syntaxin 1 reduces rapid exocytosis in insulin-secreting cells. (2006)

Vikman, Jenny, Ma, Xiaosong, Hockerman, Gregory H, Rorsman, Patrik, Eliasson, Lena

Abstract is not available

Secretory and electrophysiological characteristics of insulin cells from gastrectomized mice: Evidence for the existence of insulinotropic agents in the stomach. (2006)

Salehi, Albert, Eliasson, Lena, Ma, Xiaosong, Rorsman, Patrik, Hakanson, Rolf, Lundquist, Ingmar

Abstract is not available

CaV2.3 calcium channels control second-phase insulin release. (2005)

Jing, Xingjun, Li, Dai-Qing, Olofsson, Charlotta S, Salehi, Albert, Surve, Vikas V, Caballero, José, ...

Abstract is not available

Regulated Exocytosis and Kiss-and-Run of Synaptic-Like Microvesicles in INS-1 and Primary Rat {beta}-Cells. (2005)

Macdonald, Patrick E, Obermüller, Stefanie, Vikman, Jenny, Galvanovskis, Juris, Rorsman, Patrik, Eliasson, Lena

Abstract is not available

Selective nucleotide-release from dense-core granules in insulin-secreting cells. (2005)

Obermüller, Stefanie, Lindqvist, Anders, Karanauskaite, Jovita, Galvanovskis, Juris, Rorsman, Patrik, Barg, Sebastian

Abstract is not available

Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels. (2005)

Bengtsson, Martin, Stahlberg, Anders, Rorsman, Patrik, Kubista, Mikael

Abstract is not available

Calcium increases endocytotic vesicle size and accelerates membrane fission in insulin-secreting INS-1 cells. (2005)

Macdonald, Patrick E, Eliasson, Lena, Rorsman, Patrik

Abstract is not available

Selective nucleotide-release from dense-core granules in insulin-secreting cells (2005)

Obermüller, Stefanie, Lindqvist, Anders, Karanauskaite, Jovita, Galvanovskis, Juris, Rorsman, Patrik, Barg, Sebastian

Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of...

Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels (2005)

Bengtsson, Martin, Ståhlberg, Anders, Rorsman, Patrik, Kubista, Mikael

The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result in stochastic behavior in gene activity. Because of technical limitations...

Capacitance measurements of exocytosis in mouse pancreatic {alpha}-, {beta}- and {delta}-cells studied in intact islets of Langerhans. (2004)

Gopel, Sven, Zhang, Quan, Eliasson, Lena, Ma, Xiao-Song, Galvanovskis, Juris, Kanno, Takahiro, ...

Abstract is not available

Regulated Exocytosis of GABA-containing Synaptic-like Microvesicles in Pancreatic {beta}-cells. (2004)

Braun, Matthias, Wendt, Anna, Birnir, Bryndis, Broman, Jonas, Eliasson, Lena, Galvanovskis, Juris, ...

Abstract is not available

Glucose Inhibition of Glucagon Secretion From Rat alpha-Cells Is Mediated by GABA Released From Neighboring beta-Cells. (2004)

Wendt, Anna, Birnir, Bryndis, Buschard, Karsten, Gromada, Jesper, Salehi, Albert, Sewing, Sabine, ...

Abstract is not available

Palmitate increases L-type Ca2+ currents and the size of the readily releasable granule pool in mouse pancreatic beta-cells. (2004)

Olofsson, Charlotta S, Salehi, Albert, Holm, Cecilia, Rorsman, Patrik

Abstract is not available

The first Gla-containing contryphan: A selective L-type calcium ion channel blocker isolated from the venom of conus marmoreus. (2004)

Hansson, Karin, Ma, Xiaosong, Eliasson, Lena, Czerwiec, Eva, Furie, Bruce, Furie, Barbara C., ...

Abstract is not available

Hormone-sensitive Lipase deficiency in mouse islets abolishes neutral cholesterol ester hydrolase activity but leaves lipolysis, acylglycerides, fat oxidation, and insulin secretion intact. (2004)

Fex, Malin, Olofsson, Charlotta S., Fransson, Ulrika, Bacos, Karl, Lindvall, Håkan, Sörhede-Winzell, Maria, ...

Abstract is not available

GABAB-receptor activation inhibits exocytosis in rat pancreatic {beta}-cells by G-protein-dependent activation of calcineurin. (2004)

Braun, Matthias, Wendt, Anna, Buschard, Karsten, Salehi, Albert, Sewing, Sabine, Gromada, Jesper, ...

Abstract is not available

Palmitate Stimulation of Glucagon Secretion in Mouse Pancreatic {alpha}-Cells Results From Activation of L-Type Calcium Channels and Elevation of Cytoplasmic Calcium. (2004)

Olofsson, Charlotta S, Salehi, Albert, Holm, Cecilia, Rorsman, Patrik

Abstract is not available

Glucagon stimulates exocytosis in mouse and rat pancreatic {alpha} cells by binding to glucagon receptors. (2004)

Ma, Xiaosong, Zhang, Yang, Gromada, Jesper, Sewing, Sabine, Berggren, Per-Olof, Buschard, Karsten, ...

Abstract is not available

Type 2 diabetes mellitus: not quite exciting enough? (2004)

Ashcroft, Frances, Rorsman, Patrik

Type 2 diabetes mellitus is a serious metabolic disease that afflicts around 5% of the population in Western societies and over 150 million people worldwide. It is characterized by elevation of the...

Type-2 diabetes mellitus: not quite exciting enough? (2004)

Ashcroft, Frances, Rorsman, Patrik

Type-2 diabetes mellitus is a serious metabolic disease that afflicts around 5% of the population in Western Societies and over 150 million people worldwide. It is characterised by elevation of the...

Type 2 diabetes mellitus: not quite exciting enough? (2004)

Ashcroft, Frances, Rorsman, Patrik

Type 2 diabetes mellitus is a serious metabolic disease that afflicts around 5% of the population in Western societies and over 150 million people worldwide. It is characterized by elevation...

Type-2 diabetes mellitus: not quite exciting enough? (2004)

Ashcroft, Frances, Rorsman, Patrik

Type-2 diabetes mellitus is a serious metabolic disease that afflicts around 5% of the population in Western Societies and over 150 million people worldwide. It is characterised by elevation of the...

SUR1 Regulates PKA-independent cAMP-induced Granule Priming in Mouse Pancreatic B-cells. (2003)

Eliasson, Lena, Ma, Xiaosong, Renström, Erik, Barg, Sebastian, Berggren, Per-Olof, Galvanovskis, Juris, ...

Abstract is not available

Impaired insulin secretion and glucose tolerance in beta cell-selective Ca(V)1.2 Ca(2+) channel null mice. (2003)

Schulla, Verena, Renström, Erik, Feil, Robert, Feil, Susanne, Franklin, Isobel, Gjinovci, Asllan, ...

Abstract is not available

A Subset of 50 Secretory Granules in Close Contact With L-Type Ca(2+) Channels Accounts for First-Phase Insulin Secretion in Mouse beta-Cells. (2002)

Barg, Sebastian, Eliasson, Lena, Renström, Erik, Rorsman, Patrik

Capacitance measurements were applied to mouse pancreatic beta-cells to elucidate the cellular mechanisms underlying biphasic insulin secretion. We report here that only 10,000 granules are...

Sulfonylurea-Mediated Stimulation of Insulin Exocytosis via an ATP-Sensitive K(+) Channel--Independent Action. (2002)

Renström, Erik, Barg, Sebastian, Thévenod, Frank, Rorsman, Patrik

Several reports indicate that hypoglycemic sulfonylureas augment Ca(2+)-dependent insulin secretion via mechanisms other than inhibition of the ATP-sensitive K(+) channel. The effect involves a 65-kd...

Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells. (2002)

Barg, Sebastian, Olofsson, Charlotta S, Schriever-Abeln, Jenny, Wendt, Anna, Gebre-Medhin, Samuel, Renström, Erik, ...

Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements...

Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells. (2002)

Olofsson, Charlotta S, Göpel, Sven O, Barg, Sebastian, Galvanovskis, Juris, Ma, Xiaosong, Salehi, Albert, ...

A readily releasable pool (RRP) of granules has been proposed to underlie the first phase of insulin secretion. In the present study we combined electron microscopy, insulin secretion measurements...

The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein (1999)

Barg, Sebastian, Renström, Erik, Berggren, Per-Olof, Bertorello, Alejandro, Bokvist, Krister, Braun, Matthias, ...

Intracellular application of the sulfonylurea tolbutamide during whole-cell patch-clamp recordings stimulated exocytosis >5-fold when applied at a cytoplasmic Ca2+ concentration of 0.17 microM....

Patch-clamp studies on pancreatic glucagon- and insulin-secreting cells / (1986)

Rorsman, Patrik.

Thesis (doctoral)--Uppsala University, 1986.

The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery

Wiser, Ofer, Trus, Michael, Hernández, Ana, Renström, Erik, Barg, Sebastian, Rorsman, Patrik, ...

Although N- and P-type Ca2+ channels predominant in fast-secreting systems, Lc-type Ca2+ channels (C-class) can play a similar role in certain secretory cells and synapses. For example, in retinal...

The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic β cells is mediated by a 65-kDa mdr-like P-glycoprotein

Barg, Sebastian, Renström, Erik, Berggren, Per-Olof, Bertorello, Alejandro, Bokvist, Krister, Braun, Matthias, ...

Intracellular application of the sulfonylurea tolbutamide during whole-cell patch-clamp recordings stimulated exocytosis >5-fold when applied at a cytoplasmic Ca2+ concentration of 0.17 μM. This...

Phosphatidylinositol 4-kinase serves as a metabolic sensor and regulates priming of secretory granules in pancreatic β cells

Olsen, Hervør L., Høy, Marianne, Zhang, Wei, Bertorello, Alejandro M., Bokvist, Krister, Capito, Kirsten, ...

Insulin secretion is controlled by the β cell′s metabolic state, and the ability of the secretory granules to undergo exocytosis increases during glucose stimulation in a membrane...

Impaired insulin secretion and glucose tolerance in β cell-selective CaV1.2 Ca2+ channel null mice

Schulla, Verena, Renström, Erik, Feil, Robert, Feil, Susanne, Franklin, Isobel, Gjinovci, Asllan, ...

Insulin is secreted from pancreatic β cells in response to an elevation of cytoplasmic Ca2+ resulting from enhanced Ca2+ influx through voltage-gated Ca2+ channels. Mouse β cells express several...

Isoform-specific regulation of mood behavior and pancreatic β cell and cardiovascular function by L-type Ca2+ channels

Sinnegger-Brauns, Martina J., Hetzenauer, Alfred, Huber, Irene G., Renström, Erik, Wietzorrek, Georg, Berjukov, Stanislav, ...

Cav1.2 and Cav1.3 L-type Ca2+ channels (LTCCs) are believed to underlie Ca2+ currents in brain, pancreatic β cells, and the cardiovascular system. In the CNS, neuronal LTCCs control...

CaV2.3 calcium channels control second-phase insulin release

Jing, Xingjun, Li, Dai-Qing, Olofsson, Charlotta S., Salehi, Albert, Surve, Vikas V., Caballero, José, ...

Concerted activation of different voltage-gated Ca2+ channel isoforms may determine the kinetics of insulin release from pancreatic islets. Here we have elucidated the role of R-type CaV2.3 channels...

Oscillations, Intercellular Coupling, and Insulin Secretion in Pancreatic β Cells

MacDonald, Patrick E, Rorsman, Patrik

Insulin is a potent metabolic regulator that is released by pancreatic beta-cells, which respond to body glucose concentrations. Here the authors explain the physiological basis of insulin release.

Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels

Bengtsson, Martin, Ståhlberg, Anders, Rorsman, Patrik, Kubista, Mikael

The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result in stochastic behavior in gene activity. Because of technical limitations...

GABAB receptor activation inhibits exocytosis in rat pancreatic β-cells by G-protein-dependent activation of calcineurin

Braun, Matthias, Wendt, Anna, Buschard, Karsten, Salehi, Albert, Sewing, Sabine, Gromada, Jesper, ...

We have investigated the regulation of hormone secretion from rat pancreatic islets by the GABAB receptors (GABABRs). Inclusion of the specific GABABR antagonist CGP 55845 in the extracellular medium...

Capacitance measurements of exocytosis in mouse pancreatic α-, β- and δ-cells within intact islets of Langerhans

Göpel, Sven, Zhang, Quan, Eliasson, Lena, Ma, Xiao-Song, Galvanovskis, Juris, Kanno, Takahiro, ...

Capacitance measurements of exocytosis were applied to functionally identified α-, β- and δ-cells in intact mouse pancreatic islets. The maximum rate of capacitance increase in β-cells during a...

Palmitate increases L-type Ca2+ currents and the size of the readily releasable granule pool in mouse pancreatic β-cells

Olofsson, Charlotta S, Salehi, Albert, Holm, Cecilia, Rorsman, Patrik

We have investigated the in vitro effects of the saturated free fatty acid palmitate on mouse pancreatic β-cells by a combination of electrophysiological recordings, intracellular Ca2+ ([Ca2+]i)...

The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery

Wiser, Ofer, Trus, Michael, Hernández, Ana, Renström, Erik, Barg, Sebastian, Rorsman, Patrik, ...

Although N- and P-type Ca2+ channels predominant in fast-secreting systems, Lc-type Ca2+ channels (C-class) can play a similar role in certain secretory cells and synapses. For example, in retinal...

The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic β cells is mediated by a 65-kDa mdr-like P-glycoprotein

Barg, Sebastian, Renström, Erik, Berggren, Per-Olof, Bertorello, Alejandro, Bokvist, Krister, Braun, Matthias, ...

Intracellular application of the sulfonylurea tolbutamide during whole-cell patch-clamp recordings stimulated exocytosis >5-fold when applied at a cytoplasmic Ca2+ concentration of 0.17 μM. This...

Phosphatidylinositol 4-kinase serves as a metabolic sensor and regulates priming of secretory granules in pancreatic β cells

Olsen, Hervør L., Høy, Marianne, Zhang, Wei, Bertorello, Alejandro M., Bokvist, Krister, Capito, Kirsten, ...

Insulin secretion is controlled by the β cell′s metabolic state, and the ability of the secretory granules to undergo exocytosis increases during glucose stimulation in a membrane...

Impaired insulin secretion and glucose tolerance in β cell-selective CaV1.2 Ca2+ channel null mice

Schulla, Verena, Renström, Erik, Feil, Robert, Feil, Susanne, Franklin, Isobel, Gjinovci, Asllan, ...

Insulin is secreted from pancreatic β cells in response to an elevation of cytoplasmic Ca2+ resulting from enhanced Ca2+ influx through voltage-gated Ca2+ channels. Mouse β cells express several...

Isoform-specific regulation of mood behavior and pancreatic β cell and cardiovascular function by L-type Ca2+ channels

Sinnegger-Brauns, Martina J., Hetzenauer, Alfred, Huber, Irene G., Renström, Erik, Wietzorrek, Georg, Berjukov, Stanislav, ...

Cav1.2 and Cav1.3 L-type Ca2+ channels (LTCCs) are believed to underlie Ca2+ currents in brain, pancreatic β cells, and the cardiovascular system. In the CNS, neuronal LTCCs control...

CaV2.3 calcium channels control second-phase insulin release

Jing, Xingjun, Li, Dai-Qing, Olofsson, Charlotta S., Salehi, Albert, Surve, Vikas V., Caballero, José, ...

Concerted activation of different voltage-gated Ca2+ channel isoforms may determine the kinetics of insulin release from pancreatic islets. Here we have elucidated the role of R-type CaV2.3 channels...

Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels

Bengtsson, Martin, Ståhlberg, Anders, Rorsman, Patrik, Kubista, Mikael

The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result in stochastic behavior in gene activity. Because of technical limitations...

Oscillations, Intercellular Coupling, and Insulin Secretion in Pancreatic β Cells

MacDonald, Patrick E, Rorsman, Patrik

Insulin is a potent metabolic regulator that is released by pancreatic beta-cells, which respond to body glucose concentrations. Here the authors explain the physiological basis of insulin release.

Capacitance measurements of exocytosis in mouse pancreatic α-, β- and δ-cells within intact islets of Langerhans

Göpel, Sven, Zhang, Quan, Eliasson, Lena, Ma, Xiao-Song, Galvanovskis, Juris, Kanno, Takahiro, ...

Capacitance measurements of exocytosis were applied to functionally identified α-, β- and δ-cells in intact mouse pancreatic islets. The maximum rate of capacitance increase in β-cells during a...

GABAB receptor activation inhibits exocytosis in rat pancreatic β-cells by G-protein-dependent activation of calcineurin

Braun, Matthias, Wendt, Anna, Buschard, Karsten, Salehi, Albert, Sewing, Sabine, Gromada, Jesper, ...

We have investigated the regulation of hormone secretion from rat pancreatic islets by the GABAB receptors (GABABRs). Inclusion of the specific GABABR antagonist CGP 55845 in the extracellular medium...

Palmitate increases L-type Ca2+ currents and the size of the readily releasable granule pool in mouse pancreatic β-cells

Olofsson, Charlotta S, Salehi, Albert, Holm, Cecilia, Rorsman, Patrik

We have investigated the in vitro effects of the saturated free fatty acid palmitate on mouse pancreatic β-cells by a combination of electrophysiological recordings, intracellular Ca2+ ([Ca2+]i)...

A KATP Channel-Dependent Pathway within α Cells Regulates Glucagon Release from Both Rodent and Human Islets of Langerhans

MacDonald, Patrick E, Marinis, Yang Zhang De, Ramracheya, Reshma, Salehi, Albert, Ma, Xiaosong, Johnson, Paul R. V, ...

Glucagon, secreted from pancreatic islet α cells, stimulates gluconeogenesis and liver glycogen breakdown. The mechanism regulating glucagon release is debated, and variously attributed to neuronal...

A dominant mutation in Snap25 causes impaired vesicle trafficking, sensorimotor gating, and ataxia in the blind-drunk mouse

Jeans, Alexander F., Oliver, Peter L., Johnson, Reuben, Capogna, Marco, Vikman, Jenny, Molnár, Zoltán, ...

The neuronal soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex is essential for synaptic vesicle exocytosis, but its study has been limited by the neonatal...

Glucose-sensing mechanisms in pancreatic β-cells

MacDonald, Patrick E, Joseph, Jamie W, Rorsman, Patrik

The appropriate secretion of insulin from pancreatic β-cells is critically important to the maintenance of energy homeostasis. The β-cells must sense and respond suitably to postprandial increases...

Corelease and Differential Exit via the Fusion Pore of GABA, Serotonin, and ATP from LDCV in Rat Pancreatic β Cells

Braun, Matthias, Wendt, Anna, Karanauskaite, Jovita, Galvanovskis, Juris, Clark, Anne, MacDonald, Patrick E., ...

The release of γ-aminobutyric acid (GABA) and ATP from rat β cells was monitored using an electrophysiological assay based on overexpression GABAA or P2X2 receptor ion channels. Exocytosis of...

CaM kinase II-dependent mobilization of secretory granules underlies acetylcholine-induced stimulation of exocytosis in mouse pancreatic B-cells

Gromada, Jesper, Høy, Marianne, Renström, Erik, Bokvist, Krister, Eliasson, Lena, Göpel, Sven, ...

Measurements of cell capacitance were used to investigate the mechanisms by which acetylcholine (ACh) stimulates Ca2+-induced exocytosis in single insulin-secreting mouse pancreatic B-cells.ACh (250...

Regulated Exocytosis of GABA-containing Synaptic-like Microvesicles in Pancreatic β-cells

Braun, Matthias, Wendt, Anna, Birnir, Bryndis, Broman, Jonas, Eliasson, Lena, Galvanovskis, Juris, ...

We have explored whether γ-aminobutyric acid (GABA) is released by regulated exocytosis of GABA-containing synaptic-like microvesicles (SLMVs) in insulin-releasing rat pancreatic β-cells. To this...

Insulin Secretion: A High-affinity Ca2+ Sensor After All?

Barg, Sebastian, Rorsman, Patrik

Tolbutamide stimulates exocytosis of glucagon by inhibition of a mitochondrial-like ATP-sensitive K+ (KATP) conductance in rat pancreatic A-cells

Høy, Marianne, Olsen, Hervør L, Bokvist, Krister, Buschard, Karsten, Barg, Sebastian, Rorsman, Patrik, ...

Capacitance measurements were used to examine the effects of the sulphonylurea tolbutamide on Ca2+-dependent exocytosis in isolated glucagon-secreting rat pancreatic A-cells.When applied...

Somatostatin inhibits exocytosis in rat pancreatic α-cells by Gi2-dependent activation of calcineurin and depriming of secretory granules

Gromada, Jesper, Høy, Marianne, Buschard, Karsten, Salehi, Albert, Rorsman, Patrik

Measurements of cell capacitance were used to investigate the molecular mechanisms by which somatostatin inhibits Ca2+-induced exocytosis in single rat glucagon-secreting pancreatic...

SUR1 Regulates PKA-independent cAMP-induced Granule Priming in Mouse Pancreatic B-cells

Eliasson, Lena, Ma, Xiaosong, Renström, Erik, Barg, Sebastian, Berggren, Per-Olof, Galvanovskis, Juris, ...

Measurements of membrane capacitance were applied to dissect the cellular mechanisms underlying PKA-dependent and -independent stimulation of insulin secretion by cyclic AMP. Whereas the...

Activation of Ca2+-Dependent K+ Channels Contributes to Rhythmic Firing of Action Potentials in Mouse Pancreatic β Cells

Göpel, Sven O., Kanno, Takahiro, Barg, Sebastian, Eliasson, Lena, Galvanovskis, Juris, Renström, Erik, ...

We have applied the perforated patch whole-cell technique to β cells within intact pancreatic islets to identify the current underlying the glucose-induced rhythmic firing of action potentials....

Adrenaline Stimulates Glucagon Secretion in Pancreatic A-Cells by Increasing the Ca2+ Current and the Number of Granules Close to the L-Type Ca2+ Channels

Gromada, Jesper, Bokvist, Krister, Ding, Wei-Guang, Barg, Sebastian, Buschard, Karsten, Renström, Erik, ...

We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated...

Quantification of mRNA in single cells and modelling of RT-qPCR induced noise

Bengtsson, Martin, Hemberg, Martin, Rorsman, Patrik, Ståhlberg, Anders

Pathophysiological, Genetic and Gene Expression Features of a Novel Rodent Model of the Cardio-Metabolic Syndrome

Wallis, Robert H., Collins, Stephan C., Kaisaki, Pamela J., Argoud, Karène, Wilder, Steven P., Wallace, Karin J., ...

Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic β cells recapitulates neonatal diabetes

Girard, Christophe A., Wunderlich, F. Thomas, Shimomura, Kenju, Collins, Stephan, Kaizik, Stephan, Proks, Peter, ...

Neonatal diabetes is a rare monogenic form of diabetes that usually presents within the first six months of life. It is commonly caused by gain-of-function mutations in the genes encoding the Kir6.2...

Regulation of PKD by the MAPK p38δ in Insulin Secretion and Glucose Homeostasis

Sumara, Grzegorz, Formentini, Ivan, Collins, Stephan, Sumara, Izabela, Windak, Renata, Bodenmiller, Bernd, ...

Dysfunction and loss of insulin-producing pancreatic β cells represent hallmarks of diabetes mellitus. Here, we show that mice lacking the mitogen-activated protein kinase (MAPK) p38δ display...

pVHL is a regulator of glucose metabolism and insulin secretion in pancreatic β cells

Zehetner, Jens, Danzer, Carsten, Collins, Stephan, Eckhardt, Katrin, Gerber, Philipp A., Ballschmieter, Pia, ...

Insulin secretion from pancreatic β cells is stimulated by glucose metabolism. However, the relative importance of metabolizing glucose via mitochondrial oxidative phosphorylation versus glycolysis...

miR-375 maintains normal pancreatic α- and β-cell mass

Poy, Matthew N., Hausser, Jean, Trajkovski, Mirko, Braun, Matthias, Collins, Stephan, Rorsman, Patrik, ...

Altered growth and development of the endocrine pancreas is a frequent cause of the hyperglycemia associated with diabetes. Here we show that microRNA-375 (miR-375), which is highly expressed in...

Novel aspects of the molecular mechanisms controlling insulin secretion

Eliasson, Lena, Abdulkader, Fernando, Braun, Matthias, Galvanovskis, Juris, Hoppa, Michael B, Rorsman, Patrik

Pancreatic β-cells secrete insulin by Ca2+-dependent exocytosis of secretory granules. β-cell exocytosis involves SNARE (soluble NSF-attachment protein receptor) proteins similar to those...