Paul Babitzke

TRAP-5' stem loop interaction increases the efficiency of transcription termination in the Bacillus subtilis trpEDCFBA operon leader region (2007)

McGraw, Adam P., Bevilacqua, Philip C., Babitzke, Paul

TRAP regulates expression of the Bacillus subtilis trpEDCFBA operon by a transcription attenuation mechanism in which tryptophan-activated TRAP binds to 11 (G/U)AG repeats in the nascent trp leader...

Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E (2006)

Suzuki, Kazushi, Babitzke, Paul, Kushner, Sidney R., Romeo, Tony

In Escherichia coli, the global regulatory protein CsrA (carbon store regulator A) binds to leader segments of target mRNAs, affecting their translation and stability. CsrA activity is regulated by...

RNA sequence and secondary structure participate in high-affinity CsrA-RNA interaction (2005)

DUBEY, ASHOK K., BAKER, CAROL S., ROMEO, TONY, BABITZKE, PAUL

The global Csr regulatory system controls bacterial gene expression post-transcriptionally. CsrA of Escherichia coli is an RNA binding protein that plays a central role in repressing several...

RNA sequence and secondary structure participate in high-affinity CsrA-RNA interaction (2005)

DUBEY, ASHOK K., BAKER, CAROL S., ROMEO, TONY, BABITZKE, PAUL

The global Csr regulatory system controls bacterial gene expression post-transcriptionally. CsrA of Escherichia coli is an RNA binding protein that plays a central role in repressing several...

A 5′ RNA Stem-Loop Participates in the Transcription Attenuation Mechanism That Controls Expression of the Bacillus subtilis trpEDCFBA Operon

Sudershana, Subita, Du, Hansen, Mahalanabis, Madhumita, Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by transcription attenuation. Tryptophan-activated TRAP binds to the nascent trp leader...

Expression of the Bacillus subtilis trpEDCFBA Operon Is Influenced by Translational Coupling and Rho Termination Factor

Yakhnin, Helen, Babiarz, Joshua E., Yakhnin, Alexander V., Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by transcription attenuation and translational control mechanisms. Both mechanisms...

Regulatory Interactions of Csr Components: the RNA Binding Protein CsrA Activates csrB Transcription in Escherichia coli

Gudapaty, Seshagirirao, Suzuki, Kazushi, Wang, Xin, Babitzke, Paul, Romeo, Tony

The global regulator CsrA (carbon storage regulator) of Escherichia coli is a small RNA binding protein that represses various metabolic pathways and processes that are induced in the stationary...

trp RNA-Binding Attenuation Protein-5′ Stem-Loop RNA Interaction Is Required for Proper Transcription Attenuation Control of the Bacillus subtilis trpEDCFBA Operon

Du, Hansen, Yakhnin, Alexander V., Dharmaraj, Subramanian, Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by a novel transcription attenuation mechanism. Tryptophan-activated TRAP binds to the...

The Escherichia coli mrsC Gene Is Required for Cell Growth and mRNA Decay

Granger, Laurie L., O’Hara, Eileen B., Wang, Rong-Fu, Meffen, Frances V., Armstrong, Katherine, Yancey, Stephanie D., ...

We have identified a gene in Escherichia coli that is required for both the normal decay of mRNA and RNA synthesis. Originally designated mrsC (mRNA stability), the mrsC505 mutation described here...

NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism in vitro

Yakhnin, Alexander V., Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by transcription attenuation and translation control mechanisms. Both mechanisms require...

Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli

Suzuki, Kazushi, Wang, Xin, Weilbacher, Thomas, Pernestig, Anna-Karin, Melefors, Öjar, Georgellis, Dimitris, ...

The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm...

CsrA Regulates Translation of the Escherichia coli Carbon Starvation Gene, cstA, by Blocking Ribosome Access to the cstA Transcript

Dubey, Ashok K., Baker, Carol S., Suzuki, Kazushi, Jones, A. Daniel, Pandit, Pallavi, Romeo, Tony, ...

CsrA is a global regulator that binds to two sites in the glgCAP leader transcript, thereby blocking ribosome access to the glgC Shine-Dalgarno sequence. The upstream CsrA binding site (GCACACGGAU)...

The trp RNA-Binding Attenuation Protein of Bacillus subtilis Regulates Translation of the Tryptophan Transport Gene trpP (yhaG) by Blocking Ribosome Binding

Yakhnin, Helen, Zhang, Hong, Yakhnin, Alexander V., Babitzke, Paul

Expression of the Bacillus subtilis tryptophan biosynthetic genes (trpEDCFBA and pabA [trpG]) is regulated in response to tryptophan by TRAP, the trp RNA-binding attenuation protein. TRAP-mediated...

Recycling of a regulatory protein by degradation of the RNA to which it binds

Deikus, Gintaras, Babitzke, Paul, Bechhofer, David H.

When Bacillus subtilis is grown in the presence of excess tryptophan, transcription of the trp operon is regulated by binding of tryptophan-activated TRAP to trp leader RNA, which promotes...

Phylogenetic conservation of RNA secondary and tertiary structure in the trpEDCFBA operon leader transcript in Bacillus

SCHAAK, JANELL E., BABITZKE, PAUL, BEVILACQUA, PHILIP C.

Expression of the trpEDCFBA operon of Bacillus subtilis is regulated by transcription attenuation and translation control mechanisms. We recently determined that the B. subtilis trp leader...

RNA sequence and secondary structure participate in high-affinity CsrA–RNA interaction

DUBEY, ASHOK K., BAKER, CAROL S., ROMEO, TONY, BABITZKE, PAUL

The global Csr regulatory system controls bacterial gene expression post-transcriptionally. CsrA of Escherichia coli is an RNA binding protein that plays a central role in repressing several...

A 5′ RNA Stem-Loop Participates in the Transcription Attenuation Mechanism That Controls Expression of the Bacillus subtilis trpEDCFBA Operon

Sudershana, Subita, Du, Hansen, Mahalanabis, Madhumita, Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by transcription attenuation. Tryptophan-activated TRAP binds to the nascent trp leader...

Expression of the Bacillus subtilis trpEDCFBA Operon Is Influenced by Translational Coupling and Rho Termination Factor

Yakhnin, Helen, Babiarz, Joshua E., Yakhnin, Alexander V., Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by transcription attenuation and translational control mechanisms. Both mechanisms...

Regulatory Interactions of Csr Components: the RNA Binding Protein CsrA Activates csrB Transcription in Escherichia coli

Gudapaty, Seshagirirao, Suzuki, Kazushi, Wang, Xin, Babitzke, Paul, Romeo, Tony

The global regulator CsrA (carbon storage regulator) of Escherichia coli is a small RNA binding protein that represses various metabolic pathways and processes that are induced in the stationary...

trp RNA-Binding Attenuation Protein-5′ Stem-Loop RNA Interaction Is Required for Proper Transcription Attenuation Control of the Bacillus subtilis trpEDCFBA Operon

Du, Hansen, Yakhnin, Alexander V., Dharmaraj, Subramanian, Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by a novel transcription attenuation mechanism. Tryptophan-activated TRAP binds to the...

The Escherichia coli mrsC Gene Is Required for Cell Growth and mRNA Decay

Granger, Laurie L., O’Hara, Eileen B., Wang, Rong-Fu, Meffen, Frances V., Armstrong, Katherine, Yancey, Stephanie D., ...

We have identified a gene in Escherichia coli that is required for both the normal decay of mRNA and RNA synthesis. Originally designated mrsC (mRNA stability), the mrsC505 mutation described here...

NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism in vitro

Yakhnin, Alexander V., Babitzke, Paul

The trp RNA-binding attenuation protein (TRAP) regulates expression of the Bacillus subtilis trpEDCFBA operon by transcription attenuation and translation control mechanisms. Both mechanisms require...

Regulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli

Suzuki, Kazushi, Wang, Xin, Weilbacher, Thomas, Pernestig, Anna-Karin, Melefors, Öjar, Georgellis, Dimitris, ...

The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm...

CsrA Regulates Translation of the Escherichia coli Carbon Starvation Gene, cstA, by Blocking Ribosome Access to the cstA Transcript

Dubey, Ashok K., Baker, Carol S., Suzuki, Kazushi, Jones, A. Daniel, Pandit, Pallavi, Romeo, Tony, ...

CsrA is a global regulator that binds to two sites in the glgCAP leader transcript, thereby blocking ribosome access to the glgC Shine-Dalgarno sequence. The upstream CsrA binding site (GCACACGGAU)...

The trp RNA-Binding Attenuation Protein of Bacillus subtilis Regulates Translation of the Tryptophan Transport Gene trpP (yhaG) by Blocking Ribosome Binding

Yakhnin, Helen, Zhang, Hong, Yakhnin, Alexander V., Babitzke, Paul

Expression of the Bacillus subtilis tryptophan biosynthetic genes (trpEDCFBA and pabA [trpG]) is regulated in response to tryptophan by TRAP, the trp RNA-binding attenuation protein. TRAP-mediated...

Recycling of a regulatory protein by degradation of the RNA to which it binds

Deikus, Gintaras, Babitzke, Paul, Bechhofer, David H.

When Bacillus subtilis is grown in the presence of excess tryptophan, transcription of the trp operon is regulated by binding of tryptophan-activated TRAP to trp leader RNA, which promotes...

Phylogenetic conservation of RNA secondary and tertiary structure in the trpEDCFBA operon leader transcript in Bacillus

SCHAAK, JANELL E., BABITZKE, PAUL, BEVILACQUA, PHILIP C.

Expression of the trpEDCFBA operon of Bacillus subtilis is regulated by transcription attenuation and translation control mechanisms. We recently determined that the B. subtilis trp leader...

RNA sequence and secondary structure participate in high-affinity CsrA–RNA interaction

DUBEY, ASHOK K., BAKER, CAROL S., ROMEO, TONY, BABITZKE, PAUL

The global Csr regulatory system controls bacterial gene expression post-transcriptionally. CsrA of Escherichia coli is an RNA binding protein that plays a central role in repressing several...

Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E

Suzuki, Kazushi, Babitzke, Paul, Kushner, Sidney R., Romeo, Tony

In Escherichia coli, the global regulatory protein CsrA (carbon store regulator A) binds to leader segments of target mRNAs, affecting their translation and stability. CsrA activity is regulated by...

CsrA Inhibits Translation Initiation of Escherichia coli hfq by Binding to a Single Site Overlapping the Shine-Dalgarno Sequence▿

Baker, Carol S., Eöry, Lél A., Yakhnin, Helen, Mercante, Jeffrey, Romeo, Tony, Babitzke, Paul

Csr (carbon storage regulation) of Escherichia coli is a global regulatory system that consists of CsrA, a homodimeric RNA binding protein, two noncoding small RNAs (sRNAs; CsrB and CsrC) that...

TRAP-5′ stem–loop interaction increases the efficiency of transcription termination in the Bacillus subtilis trpEDCFBA operon leader region

McGraw, Adam P., Bevilacqua, Philip C., Babitzke, Paul

TRAP regulates expression of the Bacillus subtilis trpEDCFBA operon by a transcription attenuation mechanism in which tryptophan-activated TRAP binds to 11 (G/U)AG repeats in the nascent trp leader...

Function of the Bacillus subtilis transcription elongation factor NusG in hairpin-dependent RNA polymerase pausing in the trp leader

Yakhnin, Alexander V., Yakhnin, Helen, Babitzke, Paul

NusA and NusG are transcription elongation factors that bind to RNA polymerase (RNAP) after σ subunit release. Escherichia coli NusA (NusAEc) stimulates intrinsic termination and RNAPEc pausing,...

Molecular basis of TRAP–5′SL RNA interaction in the Bacillus subtilis trp operon transcription attenuation mechanism

McGraw, Adam P., Mokdad, Ali, Major, François, Bevilacqua, Philip C., Babitzke, Paul

Expression of the Bacillus subtilis trpEDCFBA operon is regulated by the interaction of tryptophan-activated TRAP with 11 (G/U)AG trinucleotide repeats that lie in the leader region of the nascent...

A Genome-Wide Analysis of Small Regulatory RNAs in the Human Pathogen Group A Streptococcus

Perez, Nataly, Treviño, Jeanette, Liu, Zhuyun, Ho, Siu Chun Michael, Babitzke, Paul, Sumby, Paul

The coordinated regulation of gene expression is essential for pathogens to infect and cause disease. A recently appreciated mechanism of regulation is that afforded by small regulatory RNA (sRNA)...