The relation between the membrance potential and the ion content of smooth muscle cells. (1965)
Summary in English, Dutch, and French.
Eggermont, J A, Wuytack, F, Casteels, R
The gene 2 sarcoplasmic/endoplasmic-reticulum (SR/ER) Ca2+ pump is expressed in slow skeletal and cardiac muscle, smooth muscle and non-muscle tissues. We have analysed the gene 2 Ca2+ pump mRNAs...
Functional difference between SERCA2a and SERCA2b Ca2+ pumps and their modulation by phospholamban.
Verboomen, H, Wuytack, F, De Smedt, H, Himpens, B, Casteels, R
COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca2+ pump (SERCA)2a or SERCA2b cDNA. Ca2+ uptake by microsomes from transfected cells revealed that the...
cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.
Verboomen, H, Wuytack, F, Eggermont, J A, De Jaegere, S, Missiaen, L, Raeymaekers, L, ...
Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino...
Alkalinization stimulates the purified plasma-membrane Ca2+ pump by increasing its Ca2+ affinity.
Missiaen, L, Droogmans, G, De Smedt, H, Wuytack, F, Raeymaekers, L, Casteels, R
The finding that negatively charged phospholipids activate the plasma-membrane (Ca2+ + Mg2+)-ATPase and that polycations counteract this stimulation suggest that negative charges in the environment...
Missiaen, L, Raeymaekers, L, Wuytack, F, Vrolix, M, De Smedt, H, Casteels, R
The aim of the present work was to investigate the stimulation of the plasma-membrane Ca2+-transporting ATPase by negatively charged phospholipids. The Ca2+-transporting ATPase was purified from pig...
Missiaen, L, Raeymaekers, L, Droogmans, G, Wuytack, F, Casteels, R
Negatively charged phospholipids strongly stimulate the purified plasma membrane Ca2+ pump of erythrocytes [Enyedi, Flura, Sarkadi, Gardos & Carafoli (1987) J. Biol. Chem. 262, 6425-6430] and of...
Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.
Wuytack, F, Eggermont, J A, Raeymaekers, L, Plessers, L, Casteels, R
We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2...
Vrolix, M, Raeymaekers, L, Wuytack, F, Hofmann, F, Casteels, R
The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase...
Wuytack, F, Kanmura, Y, Eggermont, J A, Raeymaekers, L, Verbist, J, Hartweg, D, ...
Smooth muscle expresses in its endoplasmic reticulum an isoform of the Ca2+-transport ATPase that is very similar to or identical with that of the cardiac-muscle/slow-twitch skeletal-muscle form....
Control of inositol 1,4,5-trisphosphate-induced Ca2+ release by cytosolic Ca2+.
Bootman, M D, Missiaen, L, Parys, J B, De Smedt, H, Casteels, R
The synergistic action of cytosolic Ca2+ and inositol 1,4,5-trisphosphate (InsP3) in releasing intracellular Ca2+ stores has been suggested to be responsible for the complex intracellular Ca2 signals...
Eggermont, J, De Smedt, H, Mertens, L, Wuytack, F, Casteels, R
Tissue-specific alternative processing of sarco/endoplasmic reticulum Ca(2+)-ATPase 2 (SERCA2) transcripts generates functionally different Ca2+ pump isoforms in muscle compared with non-muscle...
Verboomen, H, Wuytack, F, Mertens, L, Casteels, R
Ca(2+)-uptake experiments in microsomal fractions from transfected COS-1 cells have revealed a functional difference between the non-muscle SERCA2b Ca2+ pump and its muscle-specific SERCA2a splice...
Inhibition of inositol trisphosphate-induced calcium release by caffeine is prevented by ATP.
Missiaen, L, Parys, J B, De Smedt, H, Himpens, B, Casteels, R
We have investigated the effect of various methylxanthines on the basal and InsP3-stimulated unidirectional 45Ca2+ efflux from permeabilized A7r5 cells under different experimental conditions. We...
Evidence for two isoforms of the endoplasmic-reticulum Ca2+ pump in pig smooth muscle.
Eggermont, J A, Wuytack, F, De Jaegere, S, Nelles, L, Casteels, R
cDNA clones coding for the endoplasmic reticulum Ca2+-transport ATPase have been cloned from a pig smooth-muscle cDNA library. The transcripts can be divided into two classes which differ in their 3'...
Missiaen, L, Wuytack, F, De Smedt, H, Amant, F, Casteels, R
AIF4- inhibits the (Ca2+ + Mg2+)-ATPase activity of the plasma-membrane and the sarcoplasmic-reticulum Ca2+-transport ATPase [Missiaen, Wuytack, De Smedt, Vrolix & Casteels (1988) Biochem. J. 253,...
Missiaen, L, Wuytack, F, Raeymaekers, L, De Smedt, H, Casteels, R
We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin....
Evidence for the presence in smooth muscle of two types of Ca2+-transport ATPase.
Wuytack, F, Raeymaekers, L, Verbist, J, De Smedt, H, Casteels, R
Membrane fractions prepared from smooth muscle of the pig stomach (antral part) contain two Ca2+-dependent phosphoprotein intermediates belonging to different Ca2+-transport ATPases. These...
Verbist, J, Wuytack, F, Raeymaekers, L, Van Leuven, F, Cassiman, J J, Casteels, R
A monoclonal antibody (2B3) directed against the calmodulin-binding (Ca2+ + Mg2+)-dependent ATPase from pig stomach smooth muscle was prepared. This antibody reacts with a 130,000-Mr protein that...
Missiaen, L, Wuytack, F, Casteels, R
The activities of Mg2+-ATPase (Mg2+-activated ATPase), (Ca2+ + Mg2+)-activated ATPase and (Na+ + K+)-activated ATPase have been determined in microsomes (microsomal fractions) obtained from rat...
Missiaen, L, Wuytack, F, Casteels, R
The apparent Mg2+-activated ATPase activity measured by the continuous NADH-coupled enzyme assay was studied in a number of microsomal preparations obtained from smooth muscle of the myometrium from...
Raeymaekers, L, Hofmann, F, Casteels, R
Phospholamban of isolated sarcoplasmic reticulum of cardiac and smooth muscle is phosphorylated by cyclic GMP-dependent protein kinase (G-kinase). Concomitantly, the affinity of the Ca2+ pump for...
Missiaen, L, Wuytack, F, De Smedt, H, Vrolix, M, Casteels, R
The only known cellular action of AlF4- is to stimulate the G-proteins. The aim of the present work is to demonstrate that AlF4- also inhibits 'P'-type cation-transport ATPases. NaF plus AlCl3...
Eggermont, J A, Wuytack, F, Verbist, J, Casteels, R
The expression of the gene 2 sarcoplasmic/endoplasmic-reticulum Ca2(+)-pump isoforms (SERCA2a and SERCA2b) and of phospholamban was studied in pig smooth muscle of the stomach, longitudinal ileum,...
Molecular cloning and sequencing of the plasma-membrane Ca2+ pump of pig smooth muscle.
De Jaegere, S, Wuytack, F, Eggermont, J A, Verboomen, H, Casteels, R
cDNAs coding for the plasma-membrane Ca2+ pump have been isolated from a pig smooth-muscle cDNA library and sequenced. The open reading frame encodes a protein of 1220 amino acids, which corresponds...
Verbist, J, Wuytack, F, Raeymaekers, L, Casteels, R
Antibodies directed against the purified calmodulin-binding (Ca2+ + Mg2+)-ATPase [(Ca2+ + Mg2+)-dependent ATPase] from pig erythrocytes and from smooth muscle of pig stomach (antral part) were raised...
Raeymaekers, L, Wuytack, F, Eggermont, J, De Schutter, G, Casteels, R
1. A plasma-membrane fraction was isolated from the smooth muscle of the pig stomach by using differential and sucrose-density-gradient centrifugations. When the centrifugation was carried out after...
Wuytack, F, De Schutter, G, Casteels, R
The Ca2+ uptake and the (Ca2+ + Mg2+)-dependent ATPase of the porcine coronary-artery smooth-muscle microsomal fraction ('microsomes') are only slightly stimulated by calmodulin. The Ca2+ uptake...
Wuytack, F, De Schutter, G, Casteels, R
(CaMg)ATPase [(Ca2+ + Mg2+)-dependent ATPase] was partially purified from a microsomal fraction of the smooth muscle of the pig stomach (antrum). Membranes were solubilized with deoxycholate,...
Missiaen, L, De Smedt, H, Parys, J B, Raeymaekers, L, Droogmans, G, ...
We have investigated the detailed kinetics of the passive Ca2+ leak from non-mitochondrial Ca2+ stores in permeabilized A7r5 cells. The decrease in the content of stored Ca2+ in the presence of 2...
Dode, L, Wuytack, F, Kools, P F, Baba-Aissa, F, Raeymaekers, L, Briké, F, ...
cDNA and genomic clones encoding human sarco/endoplasmic reticulum Ca(2+)-ATPase 3 (SERCA3) were isolated. The composite nucleotide sequence of the 4.6 kb cDNA, as well as the partial structure of 25...
Isoform diversity of the inositol trisphosphate receptor in cell types of mouse origin.
De Smedt, H, Missiaen, L, Parys, J B, Henning, R H, Sienaert, I, Vanlingen, S, ...
Previous reports suggested the expression of four or five different Ins(1,4,5)P3 receptor [Ins(1,4,5)P3R] isoforms in mouse cells [Ross, Danoff, Schell, Snyder and Ullrich (1992) Proc. Natl. Acad....
Missiaen, L, De Smedt, H, Parys, J B, Sienaert, I, Sipma, H, Vanlingen, S, ...
Inositol 1,4,5-trisphosphate (InsP3)-induced Ca2+ release from intracellular stores is generally assumed to be a 'quantal' process because low InsP3 concentrations mobilize less Ca2+ than high...
Effect of adenine nucleotides on myo-inositol-1,4,5-trisphosphate-induced calcium release.
Missiaen, L, Parys, J B, Smedt, H D, Sienaert, I, Sipma, H, Vanlingen, S, ...
The effects of a whole series of adenine nucleotides on Ins(1,4,5)P3-induced Ca2+ release were characterized in permeabilized A7r5 smooth-muscle cells. Several adenine nucleotides activated the...
Missiaen, L, Parys, J B, De Smedt, H, Sienaert, I, Sipma, H, Vanlingen, S, ...
Ca2+ release from intracellular stores occurs via two families of intracellular channels, each with their own specific agonist: Ins(1, 4,5)P3 for the Ins(1,4,5)P3 receptor and cyclic ADP-ribose...
ATP-induced Ca2+ release and Cl- current in cultured smooth muscle cells from pig aorta.
Droogmans, G, Callewaert, G, Declerck, I, Casteels, R
1. The effect of exogenous ATP on transmembrane currents and on the cytoplasmic Ca2+ has been investigated in single cultured smooth muscle cells of pig aorta. 2. ATP applied to cells held at a...
Missiaen, L, Declerck, I, Droogmans, G, Plessers, L, De Smedt, H, Raeymaekers, L, ...
1. The properties of intracellular Ca2+ stores of intact- and of saponin-skinned A7r5 (an established cell line from embryonic rat aorta) smooth muscle cells were studied by measuring 45Ca2+ and...
The effect of K+ depolarization and agonists on the 86Rb+ efflux from rabbit ear artery has been investigated. K+ depolarization with 59 mM-K+ induces an increase of the 86Rb+ efflux rate, which is...
Reserpine has a direct action as a calcium antagonist on mammalian smooth muscle cells.
The effects of reserpine on excitation-contraction coupling and 45Ca exchange of smooth muscle cells of the rabbit ear artery and the guinea-pig taenia coli have been studied. Reserpine inhibited the...
1. The amplitude of the noradrenaline-sensitive Ca stores has been estimated by measuring the amplitude of the transient contraction induced by the agonist in Ca-free solution. or by measuring the...
1. The role of an intracellular Ca store in excitation-contraction coupling was studied by recording isometric tension development of thin strips (100-150 micron in diameter) of taenia coli incubated...
Casteels, R., Droogmans, G., Hendrickx, H.
1. The changes of the ion content and of the membrane potential of taenia coli cells have been studied during prolonged exposure to Na-deficient solutions containing either Li or choline.
Membrane potential of smooth muscle cells in K-free solution
Casteels, R., Droogmans, G., Hendrickx, H.
1. The changes of the ion content, the membrane potential and of the membrane permeability of taenia coli cells have been studied during exposure to K-free solutions. The relative value of the total...
Electrogenic sodium pump in smooth muscle cells of the guinea-pig's taenia coli
Casteels, R., Droogmans, G., Hendrickx, H.
1. The changes of the membrane potential, of the K equilibrium potential, and of the membrane conductance during K accumulation by K-depleted tissues have been studied. Three subsequent...
The distribution of chloride ions in the smooth muscle cells of the guinea-pig's taenia coli
1. The intracellular Cl concentration of taenia coli cells, determined by an analytical procedure and by an extrapolation procedure, has a value between 60 and 73 m-mole/l. cell water.
1. The O2 consumption and lactic acid production of the guinea-pig's taenia coli have been studied in relation to the active Na-K transport, in order to estimate the ratio: active Na extrusion/active...
1. The intracellular K+, Cl- and Na+ concentrations in the taenia coli cells of the guinea-pig have been estimated from the total ion content or the extrapolated intracellular tracer content, the...
The membrane properties of the smooth muscle cells of the rabbit main pulmonary artery
Casteels, R., Kitamura, K., Kuriyama, H., Suzuki, H.
1. The membrane potential of the smooth muscle cells of the rabbit main pulmonary artery amounts to -57 mV, the length constant of the tissue is 1·48 mm and the time constant of the membrane 182...
Excitation—contraction coupling in the smooth muscle cells of the rabbit main pulmonary artery
Casteels, R., Kitamura, K., Kuriyama, H., Suzuki, H.
1. Increasing the external K concentration depolarizes the smooth muscle cells of the main pulmonary artery, and this depolarization reaches a maximal slope of 58 mV for a tenfold change of [K]o. The...
1. The relation between the membrane potential and the intracellular ion concentration of the taenia coli was studied in solutions with different external potassium concentrations.
The action of ouabain on the smooth muscle cells of the guinea-pig's taenia coli
1. The action of 10-6 g/ml. of ouabain on the membrane potential, membrane activity, tension development and the ion content of the smooth muscle of the guinea-pig's taenia coli has been investigated.
Eggermont, J A, Wuytack, F, Casteels, R
The gene 2 sarcoplasmic/endoplasmic-reticulum (SR/ER) Ca2+ pump is expressed in slow skeletal and cardiac muscle, smooth muscle and non-muscle tissues. We have analysed the gene 2 Ca2+ pump mRNAs...
Functional difference between SERCA2a and SERCA2b Ca2+ pumps and their modulation by phospholamban.
Verboomen, H, Wuytack, F, De Smedt, H, Himpens, B, Casteels, R
COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca2+ pump (SERCA)2a or SERCA2b cDNA. Ca2+ uptake by microsomes from transfected cells revealed that the...
cDNA cloning and sequencing of phospholamban from pig stomach smooth muscle.
Verboomen, H, Wuytack, F, Eggermont, J A, De Jaegere, S, Missiaen, L, Raeymaekers, L, ...
Phospholamban cDNA from pig stomach smooth muscle was cloned and sequenced. The 737-nucleotide-residue cDNA contained an open reading frame of 156 nucleotide residues encoding a peptide of 52 amino...
Alkalinization stimulates the purified plasma-membrane Ca2+ pump by increasing its Ca2+ affinity.
Missiaen, L, Droogmans, G, De Smedt, H, Wuytack, F, Raeymaekers, L, Casteels, R
The finding that negatively charged phospholipids activate the plasma-membrane (Ca2+ + Mg2+)-ATPase and that polycations counteract this stimulation suggest that negative charges in the environment...
Missiaen, L, Raeymaekers, L, Wuytack, F, Vrolix, M, De Smedt, H, Casteels, R
The aim of the present work was to investigate the stimulation of the plasma-membrane Ca2+-transporting ATPase by negatively charged phospholipids. The Ca2+-transporting ATPase was purified from pig...
Missiaen, L, Raeymaekers, L, Droogmans, G, Wuytack, F, Casteels, R
Negatively charged phospholipids strongly stimulate the purified plasma membrane Ca2+ pump of erythrocytes [Enyedi, Flura, Sarkadi, Gardos & Carafoli (1987) J. Biol. Chem. 262, 6425-6430] and of...
Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.
Wuytack, F, Eggermont, J A, Raeymaekers, L, Plessers, L, Casteels, R
We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2...
Vrolix, M, Raeymaekers, L, Wuytack, F, Hofmann, F, Casteels, R
The effect of phosphorylation by cyclic GMP-dependent protein kinase (G-kinase) on the activity of the plasmalemmal Ca2+-transport ATPase was studied on isolated plasma membranes and on the ATPase...
Wuytack, F, Kanmura, Y, Eggermont, J A, Raeymaekers, L, Verbist, J, Hartweg, D, ...
Smooth muscle expresses in its endoplasmic reticulum an isoform of the Ca2+-transport ATPase that is very similar to or identical with that of the cardiac-muscle/slow-twitch skeletal-muscle form....
Control of inositol 1,4,5-trisphosphate-induced Ca2+ release by cytosolic Ca2+.
Bootman, M D, Missiaen, L, Parys, J B, De Smedt, H, Casteels, R
The synergistic action of cytosolic Ca2+ and inositol 1,4,5-trisphosphate (InsP3) in releasing intracellular Ca2+ stores has been suggested to be responsible for the complex intracellular Ca2 signals...
Eggermont, J, De Smedt, H, Mertens, L, Wuytack, F, Casteels, R
Tissue-specific alternative processing of sarco/endoplasmic reticulum Ca(2+)-ATPase 2 (SERCA2) transcripts generates functionally different Ca2+ pump isoforms in muscle compared with non-muscle...
Verboomen, H, Wuytack, F, Mertens, L, Casteels, R
Ca(2+)-uptake experiments in microsomal fractions from transfected COS-1 cells have revealed a functional difference between the non-muscle SERCA2b Ca2+ pump and its muscle-specific SERCA2a splice...
Inhibition of inositol trisphosphate-induced calcium release by caffeine is prevented by ATP.
Missiaen, L, Parys, J B, De Smedt, H, Himpens, B, Casteels, R
We have investigated the effect of various methylxanthines on the basal and InsP3-stimulated unidirectional 45Ca2+ efflux from permeabilized A7r5 cells under different experimental conditions. We...
Evidence for two isoforms of the endoplasmic-reticulum Ca2+ pump in pig smooth muscle.
Eggermont, J A, Wuytack, F, De Jaegere, S, Nelles, L, Casteels, R
cDNA clones coding for the endoplasmic reticulum Ca2+-transport ATPase have been cloned from a pig smooth-muscle cDNA library. The transcripts can be divided into two classes which differ in their 3'...
Missiaen, L, Wuytack, F, De Smedt, H, Amant, F, Casteels, R
AIF4- inhibits the (Ca2+ + Mg2+)-ATPase activity of the plasma-membrane and the sarcoplasmic-reticulum Ca2+-transport ATPase [Missiaen, Wuytack, De Smedt, Vrolix & Casteels (1988) Biochem. J. 253,...
Missiaen, L, Wuytack, F, Raeymaekers, L, De Smedt, H, Casteels, R
We investigated the effect of spermine, spermidine, putrescine and neomycin on the activity of the plasma-membrane Ca2+ pump and on its stimulation by negatively charged phospholipids and calmodulin....
Evidence for the presence in smooth muscle of two types of Ca2+-transport ATPase.
Wuytack, F, Raeymaekers, L, Verbist, J, De Smedt, H, Casteels, R
Membrane fractions prepared from smooth muscle of the pig stomach (antral part) contain two Ca2+-dependent phosphoprotein intermediates belonging to different Ca2+-transport ATPases. These...
Verbist, J, Wuytack, F, Raeymaekers, L, Van Leuven, F, Cassiman, J J, Casteels, R
A monoclonal antibody (2B3) directed against the calmodulin-binding (Ca2+ + Mg2+)-dependent ATPase from pig stomach smooth muscle was prepared. This antibody reacts with a 130,000-Mr protein that...
Missiaen, L, Wuytack, F, Casteels, R
The activities of Mg2+-ATPase (Mg2+-activated ATPase), (Ca2+ + Mg2+)-activated ATPase and (Na+ + K+)-activated ATPase have been determined in microsomes (microsomal fractions) obtained from rat...
Missiaen, L, Wuytack, F, Casteels, R
The apparent Mg2+-activated ATPase activity measured by the continuous NADH-coupled enzyme assay was studied in a number of microsomal preparations obtained from smooth muscle of the myometrium from...
Raeymaekers, L, Hofmann, F, Casteels, R
Phospholamban of isolated sarcoplasmic reticulum of cardiac and smooth muscle is phosphorylated by cyclic GMP-dependent protein kinase (G-kinase). Concomitantly, the affinity of the Ca2+ pump for...
Missiaen, L, Wuytack, F, De Smedt, H, Vrolix, M, Casteels, R
The only known cellular action of AlF4- is to stimulate the G-proteins. The aim of the present work is to demonstrate that AlF4- also inhibits 'P'-type cation-transport ATPases. NaF plus AlCl3...
Eggermont, J A, Wuytack, F, Verbist, J, Casteels, R
The expression of the gene 2 sarcoplasmic/endoplasmic-reticulum Ca2(+)-pump isoforms (SERCA2a and SERCA2b) and of phospholamban was studied in pig smooth muscle of the stomach, longitudinal ileum,...
Molecular cloning and sequencing of the plasma-membrane Ca2+ pump of pig smooth muscle.
De Jaegere, S, Wuytack, F, Eggermont, J A, Verboomen, H, Casteels, R
cDNAs coding for the plasma-membrane Ca2+ pump have been isolated from a pig smooth-muscle cDNA library and sequenced. The open reading frame encodes a protein of 1220 amino acids, which corresponds...
Verbist, J, Wuytack, F, Raeymaekers, L, Casteels, R
Antibodies directed against the purified calmodulin-binding (Ca2+ + Mg2+)-ATPase [(Ca2+ + Mg2+)-dependent ATPase] from pig erythrocytes and from smooth muscle of pig stomach (antral part) were raised...
Raeymaekers, L, Wuytack, F, Eggermont, J, De Schutter, G, Casteels, R
1. A plasma-membrane fraction was isolated from the smooth muscle of the pig stomach by using differential and sucrose-density-gradient centrifugations. When the centrifugation was carried out after...
Wuytack, F, De Schutter, G, Casteels, R
The Ca2+ uptake and the (Ca2+ + Mg2+)-dependent ATPase of the porcine coronary-artery smooth-muscle microsomal fraction ('microsomes') are only slightly stimulated by calmodulin. The Ca2+ uptake...
Wuytack, F, De Schutter, G, Casteels, R
(CaMg)ATPase [(Ca2+ + Mg2+)-dependent ATPase] was partially purified from a microsomal fraction of the smooth muscle of the pig stomach (antrum). Membranes were solubilized with deoxycholate,...
ATP-induced Ca2+ release and Cl- current in cultured smooth muscle cells from pig aorta.
Droogmans, G, Callewaert, G, Declerck, I, Casteels, R
1. The effect of exogenous ATP on transmembrane currents and on the cytoplasmic Ca2+ has been investigated in single cultured smooth muscle cells of pig aorta. 2. ATP applied to cells held at a...
Missiaen, L, Declerck, I, Droogmans, G, Plessers, L, De Smedt, H, Raeymaekers, L, ...
1. The properties of intracellular Ca2+ stores of intact- and of saponin-skinned A7r5 (an established cell line from embryonic rat aorta) smooth muscle cells were studied by measuring 45Ca2+ and...
The effect of K+ depolarization and agonists on the 86Rb+ efflux from rabbit ear artery has been investigated. K+ depolarization with 59 mM-K+ induces an increase of the 86Rb+ efflux rate, which is...
Reserpine has a direct action as a calcium antagonist on mammalian smooth muscle cells.
The effects of reserpine on excitation-contraction coupling and 45Ca exchange of smooth muscle cells of the rabbit ear artery and the guinea-pig taenia coli have been studied. Reserpine inhibited the...
Missiaen, L, De Smedt, H, Parys, J B, Raeymaekers, L, Droogmans, G, ...
We have investigated the detailed kinetics of the passive Ca2+ leak from non-mitochondrial Ca2+ stores in permeabilized A7r5 cells. The decrease in the content of stored Ca2+ in the presence of 2...
Dode, L, Wuytack, F, Kools, P F, Baba-Aissa, F, Raeymaekers, L, Briké, F, ...
cDNA and genomic clones encoding human sarco/endoplasmic reticulum Ca(2+)-ATPase 3 (SERCA3) were isolated. The composite nucleotide sequence of the 4.6 kb cDNA, as well as the partial structure of 25...
Isoform diversity of the inositol trisphosphate receptor in cell types of mouse origin.
De Smedt, H, Missiaen, L, Parys, J B, Henning, R H, Sienaert, I, Vanlingen, S, ...
Previous reports suggested the expression of four or five different Ins(1,4,5)P3 receptor [Ins(1,4,5)P3R] isoforms in mouse cells [Ross, Danoff, Schell, Snyder and Ullrich (1992) Proc. Natl. Acad....
Missiaen, L, De Smedt, H, Parys, J B, Sienaert, I, Sipma, H, Vanlingen, S, ...
Inositol 1,4,5-trisphosphate (InsP3)-induced Ca2+ release from intracellular stores is generally assumed to be a 'quantal' process because low InsP3 concentrations mobilize less Ca2+ than high...
Effect of adenine nucleotides on myo-inositol-1,4,5-trisphosphate-induced calcium release.
Missiaen, L, Parys, J B, Smedt, H D, Sienaert, I, Sipma, H, Vanlingen, S, ...
The effects of a whole series of adenine nucleotides on Ins(1,4,5)P3-induced Ca2+ release were characterized in permeabilized A7r5 smooth-muscle cells. Several adenine nucleotides activated the...
Missiaen, L, Parys, J B, De Smedt, H, Sienaert, I, Sipma, H, Vanlingen, S, ...
Ca2+ release from intracellular stores occurs via two families of intracellular channels, each with their own specific agonist: Ins(1, 4,5)P3 for the Ins(1,4,5)P3 receptor and cyclic ADP-ribose...
1. The amplitude of the noradrenaline-sensitive Ca stores has been estimated by measuring the amplitude of the transient contraction induced by the agonist in Ca-free solution. or by measuring the...
1. The role of an intracellular Ca store in excitation-contraction coupling was studied by recording isometric tension development of thin strips (100-150 micron in diameter) of taenia coli incubated...
Casteels, R., Droogmans, G., Hendrickx, H.
1. The changes of the ion content and of the membrane potential of taenia coli cells have been studied during prolonged exposure to Na-deficient solutions containing either Li or choline.
Membrane potential of smooth muscle cells in K-free solution
Casteels, R., Droogmans, G., Hendrickx, H.
1. The changes of the ion content, the membrane potential and of the membrane permeability of taenia coli cells have been studied during exposure to K-free solutions. The relative value of the total...
Electrogenic sodium pump in smooth muscle cells of the guinea-pig's taenia coli
Casteels, R., Droogmans, G., Hendrickx, H.
1. The changes of the membrane potential, of the K equilibrium potential, and of the membrane conductance during K accumulation by K-depleted tissues have been studied. Three subsequent...
The distribution of chloride ions in the smooth muscle cells of the guinea-pig's taenia coli
1. The intracellular Cl concentration of taenia coli cells, determined by an analytical procedure and by an extrapolation procedure, has a value between 60 and 73 m-mole/l. cell water.
1. The O2 consumption and lactic acid production of the guinea-pig's taenia coli have been studied in relation to the active Na-K transport, in order to estimate the ratio: active Na extrusion/active...
1. The intracellular K+, Cl- and Na+ concentrations in the taenia coli cells of the guinea-pig have been estimated from the total ion content or the extrapolated intracellular tracer content, the...
The membrane properties of the smooth muscle cells of the rabbit main pulmonary artery
Casteels, R., Kitamura, K., Kuriyama, H., Suzuki, H.
1. The membrane potential of the smooth muscle cells of the rabbit main pulmonary artery amounts to -57 mV, the length constant of the tissue is 1·48 mm and the time constant of the membrane 182...
Excitation—contraction coupling in the smooth muscle cells of the rabbit main pulmonary artery
Casteels, R., Kitamura, K., Kuriyama, H., Suzuki, H.
1. Increasing the external K concentration depolarizes the smooth muscle cells of the main pulmonary artery, and this depolarization reaches a maximal slope of 58 mV for a tenfold change of [K]o. The...
1. The relation between the membrane potential and the intracellular ion concentration of the taenia coli was studied in solutions with different external potassium concentrations.
The action of ouabain on the smooth muscle cells of the guinea-pig's taenia coli
1. The action of 10-6 g/ml. of ouabain on the membrane potential, membrane activity, tension development and the ion content of the smooth muscle of the guinea-pig's taenia coli has been investigated.
Bülbring, Edith, Casteels, R., Kuriyama, H.
1. Cats, virgin and 17 days pregnant, and guinea-pigs, virgin and 14-60 days pregnant, or treated for 1-8 days with oestradiol+progesterone, were used. The response of the uterus to adrenaline and...
Properties of intracellular calcium stores in pregnant rat myometrium.
Kanmura, Y., Missiaen, L., Casteels, R.
1. The properties of the Ca2+ stores in myometrium of 21-day pregnant rats were studied by recording the contractile responses of saponin-treated skinned muscles. 2. After accumulation of Ca2+ into...
Ketamine-induced relaxation in intact and skinned smooth muscles of the rabbit ear artery.
Kanmura, Y., Yoshitake, J., Casteels, R.
1. The effects of ketamine, an intravenous anaesthetic, on the rabbit ear artery were investigated by measuring the tension in intact and saponin-treated skinned smooth-muscle fibres. 2. Ketamine...
Boland, B., Himpens, B., Vincent, M. F., Gillis, J. M., Casteels, R.
1. The mechanism for the low potency of exogenous ATP in producing contraction at the P2x-purinoceptors in the smooth muscle of the mouse vas deferens (VD) was examined. 2. The measure of the...
ATP induced-relaxation in the mouse bladder smooth muscle.
Boland, B., Himpens, B., Paques, C., Casteels, R., Gillis, J. M.
1. The effect of adenosine 5'-triphosphate (ATP) on the free cytosolic Ca2+ concentration ([Ca2+]i) as measured with the fluorescent Ca(2+)-indicator fura-2, and on force was investigated in the...
Post-receptor pathway of the ATP-induced relaxation in smooth muscle of the mouse vas deferens.
Gailly, P., Boland, B., Paques, C., Himpens, B., Casteels, R., Gillis, J. M.
1. The post-receptor pathway of the ATP relaxant effect in K(+)-precontracted vas deferens smooth muscle (VD) was examined. 2. The relaxation to ATP was not antagonized either by 10 microM methylene...