The Hopkins Review - Volume 2, Number 1, Winter 2009 (New Series)
Low-Thrust Bipropellant Engine Technology. (2002)
The design, fabrication and hotfire test of a low-thrust, 0.5 lbF class bipropellant rocket engine, capable of providing very small, precisely repeatible impulse bits and long, high-performance,...
ROTHR Mode-Linking Using Neural Networks (2002)
A novel concept using neural network technology was employed to find the correct linkage of ionospheic modes in a multi-mode multi-target environment given a model of the ionosphere. The technology...
DeShazer, D, Wood, G E, Friedman, R L
Transcription of the pertussis toxin operon (ptx) is positively regulated in Bordetella pertussis by the bvgAS locus. However, a ptx-lacZ transcriptional fusion in Escherichia coli cannot be...
Bannan, J D, Moran, M J, MacInnes, J I, Soltes, G A, Friedman, R L
To determine whether hemolytic factors other than the bifunctional hemolysin-adenylate cyclase toxin (cyclolysin) are expressed by Bordetella pertussis, a gene library was constructed from a virulent...
Gotsick, P S, Friedman, R L, Stith, J B
A demonstration core collection of twenty-four ready-reference sources and five journals was selected cooperatively by a solo practitioner in rural Menifee County, Kentucky, and the University of...
Bordetella pertussis induces respiratory burst activity in human polymorphonuclear leukocytes.
Steed, L L, Akporiaye, E T, Friedman, R L
Virulent Bordetella pertussis strains survive intracellularly within human polymorphonuclear leukocytes (PMN), at least in part because of inhibition of phagosome-lysosome fusion (L. L. Steed, M....
Uptake and intracellular survival of Bordetella pertussis in human macrophages.
Friedman, R L, Nordensson, K, Wilson, L, Akporiaye, E T, Yocum, D E
Recent reports have demonstrated that Bordetella pertussis has invasive behavior in vivo and in vitro. In this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants...
Galgiani, J N, Hewlett, E L, Friedman, R L
Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by...
Purified preparations of adenylate cyclase were obtained from crude urea extracts of Bordetella pertussis by a one-step calmodulin affinity chromatography technique. Diluted extract was loaded onto...
Friedman, R L, Fiederlein, R L, Glasser, L, Galgiani, J N
Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrated to have biological effects on human polymorphonuclear leukocytes (PMN). AC at...
Friedman, R L, Paulaitis, S, McMillan, J W
Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot...
Leusch, M S, Paulaitis, S, Friedman, R L
Bordetella pertussis produces a number of virulence determinants which contribute to its pathogenicity. One factor, the adenylate cyclase toxin (ACT), has been suggested to directly penetrate human...
Cryz, S J, Friedman, R L, Iglewski, B H
Nitrosoguanidine mutagenesis of Pseudomonas aeruginosa strain PAO-1 yielded a mutant strain, PAO-PR1, which produced a protein that was immunologically indistinguishable from native toxin A and was...
Cryz, S J, Friedman, R L, Pavlovskis, O R, Iglewski, B H
We investigated the effect of Formalin toxoiding on the biological, chemical, and immunological activities of Pseudomonas aeruginosa toxin A. Formalin treatment alone resulted in a 1,000-fold...
Pertussis: the disease and new diagnostic methods.
Bordetella pertussis, the causative agent of whooping cough, produces an acute and chronic respiratory infection in infants and young children. B. pertussis is still a major health problem of young...
Cloning of cDNA sequences of human adenosine deaminase.
Wiginton, D A, Adrian, G S, Friedman, R L, Suttle, D P, Hutton, J J
Cloned cDNA sequences of human adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) have been isolated from a cDNA library constructed in bacteriophage lambda gt10. The cDNA for the...
Expression of human adenosine deaminase using a transmissable murine retrovirus vector system.
Human adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) was expressed at high levels in cultured mouse cells using a transmissable murine retrovirus vector system. A cDNA clone encoding...
Hepatic clearance of Salmonella typhimurium in silica-treated mice.
Scanning electron microscopy demonstrates that crystalline silica destroys liver Kupffer cells but has no other obvious deleterious effects on the liver. Silica-treated livers still retain the...
Identification of a cytotoxin produced by Legionella pneumophila.
Friedman, R L, Iglewski, B H, Miller, R D
Culture filtrates of Legionella pneumophila were cytotoxic for Chinese hamster ovary cells. The cytotoxin was found to be methanol soluble, heat stable, and stable from pH 5 through 8.
Woods, D E, Cryz, S J, Friedman, R L, Iglewski, B H
A chronic pulmonary infection model in rats was employed to assess the role of individual Pseudomonas aeruginosa exoproducts in disease due to this organism. Groups of rats were inoculated...
DeShazer, D, Wood, G E, Friedman, R L
Transcription of the pertussis toxin operon (ptx) is positively regulated in Bordetella pertussis by the bvgAS locus. However, a ptx-lacZ transcriptional fusion in Escherichia coli cannot be...
Bannan, J D, Moran, M J, MacInnes, J I, Soltes, G A, Friedman, R L
To determine whether hemolytic factors other than the bifunctional hemolysin-adenylate cyclase toxin (cyclolysin) are expressed by Bordetella pertussis, a gene library was constructed from a virulent...
Gotsick, P S, Friedman, R L, Stith, J B
A demonstration core collection of twenty-four ready-reference sources and five journals was selected cooperatively by a solo practitioner in rural Menifee County, Kentucky, and the University of...
Bordetella pertussis induces respiratory burst activity in human polymorphonuclear leukocytes.
Steed, L L, Akporiaye, E T, Friedman, R L
Virulent Bordetella pertussis strains survive intracellularly within human polymorphonuclear leukocytes (PMN), at least in part because of inhibition of phagosome-lysosome fusion (L. L. Steed, M....
Uptake and intracellular survival of Bordetella pertussis in human macrophages.
Friedman, R L, Nordensson, K, Wilson, L, Akporiaye, E T, Yocum, D E
Recent reports have demonstrated that Bordetella pertussis has invasive behavior in vivo and in vitro. In this study, we investigated the ability of a virulent strain, avirulent mutants, and mutants...
Galgiani, J N, Hewlett, E L, Friedman, R L
Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by...
Purified preparations of adenylate cyclase were obtained from crude urea extracts of Bordetella pertussis by a one-step calmodulin affinity chromatography technique. Diluted extract was loaded onto...
Friedman, R L, Fiederlein, R L, Glasser, L, Galgiani, J N
Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrated to have biological effects on human polymorphonuclear leukocytes (PMN). AC at...
Friedman, R L, Paulaitis, S, McMillan, J W
Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot...
Leusch, M S, Paulaitis, S, Friedman, R L
Bordetella pertussis produces a number of virulence determinants which contribute to its pathogenicity. One factor, the adenylate cyclase toxin (ACT), has been suggested to directly penetrate human...
Cryz, S J, Friedman, R L, Iglewski, B H
Nitrosoguanidine mutagenesis of Pseudomonas aeruginosa strain PAO-1 yielded a mutant strain, PAO-PR1, which produced a protein that was immunologically indistinguishable from native toxin A and was...
Cryz, S J, Friedman, R L, Pavlovskis, O R, Iglewski, B H
We investigated the effect of Formalin toxoiding on the biological, chemical, and immunological activities of Pseudomonas aeruginosa toxin A. Formalin treatment alone resulted in a 1,000-fold...
Pertussis: the disease and new diagnostic methods.
Bordetella pertussis, the causative agent of whooping cough, produces an acute and chronic respiratory infection in infants and young children. B. pertussis is still a major health problem of young...
Cloning of cDNA sequences of human adenosine deaminase.
Wiginton, D A, Adrian, G S, Friedman, R L, Suttle, D P, Hutton, J J
Cloned cDNA sequences of human adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) have been isolated from a cDNA library constructed in bacteriophage lambda gt10. The cDNA for the...
Expression of human adenosine deaminase using a transmissable murine retrovirus vector system.
Human adenosine deaminase (ADA; adenosine aminohydrolase, EC 3.5.4.4) was expressed at high levels in cultured mouse cells using a transmissable murine retrovirus vector system. A cDNA clone encoding...
Hepatic clearance of Salmonella typhimurium in silica-treated mice.
Scanning electron microscopy demonstrates that crystalline silica destroys liver Kupffer cells but has no other obvious deleterious effects on the liver. Silica-treated livers still retain the...
Identification of a cytotoxin produced by Legionella pneumophila.
Friedman, R L, Iglewski, B H, Miller, R D
Culture filtrates of Legionella pneumophila were cytotoxic for Chinese hamster ovary cells. The cytotoxin was found to be methanol soluble, heat stable, and stable from pH 5 through 8.
Woods, D E, Cryz, S J, Friedman, R L, Iglewski, B H
A chronic pulmonary infection model in rats was employed to assess the role of individual Pseudomonas aeruginosa exoproducts in disease due to this organism. Groups of rats were inoculated...