Ralf Herwig

Details der Publikationsliste

Zeitraum

1984 - 2009

Anzahl

81

Co-Autoren

In silicoidentification of a core regulatory network of OCT4 in human embryonic stem cells using an integrated approach (2009)

Chavez, Lukas, Bais, Abha S, Vingron, Martin, Lehrach, Hans, Adjaye, James, Herwig, Ralf

Abstract Background The transcription factor OCT4 is highly expressed in pluripotent embryonic stem cells which are derived from the inner cell mass of mammalian blastocysts. Pluripotency and self...

Reverse Engineering of Gene Regulatory Networks: A Comparative Study (2009)

Hendrik Hache, Hans Lehrach, Ralf Herwig

Reverse engineering of gene regulatory networks has been an intensively studied topic in bioinformatics since it constitutes an intermediate step from explorative to causative gene expression...

ConsensusPathDB--a database for integrating human functional interaction networks (2009)

Kamburov, Atanas, Wierling, Christoph, Lehrach, Hans, Herwig, Ralf

ConsensusPathDB is a database system for the integration of human functional interactions. Current knowledge of these interactions is dispersed in more than 200 databases, each having a specific...

GeNGe: systematic generation of gene regulatory networks (2009)

Hache, Hendrik, Wierling, Christoph, Lehrach, Hans, Herwig, Ralf

Summary: The analysis of gene regulatory networks (GRNs) is a central goal of bioinformatics highly accelerated by the advent of new experimental techniques, such as RNA interference. A battery of...

Reverse Engineering of Gene Regulatory Networks: A Comparative Study (2009)

Hendrik Hache, Hans Lehrach, Ralf Herwig

Reverse engineering of gene regulatory networks has been an intensively studied topic in bioinformatics since it constitutes an intermediate step from explorative to causative gene expression...

Meta-Analysis Approach identifies Candidate Genes and associated Molecular Networks for Type-2 Diabetes Mellitus (2008)

Rasche, Axel, Al-Hasani, Hadi, Herwig, Ralf

Abstract Background Multiple functional genomics data for complex human diseases have been published and made available by researchers worldwide. The main goal of these studies is the detailed...

ConsensusPathDB—a database for integrating human functional interaction networks (2008)

Kamburov, Atanas, Wierling, Christoph, Lehrach, Hans, Herwig, Ralf

ConsensusPathDB is a database system for the integration of human functional interactions. Current knowledge of these interactions is dispersed in more than 200 databases, each having a specific...

Meta-analysis approach identifies candidate genes and associated molecular networks for Type-2 Diabetes Mellitus (2008)

Rasche, Axel, Al-Hasani, Hadi, Herwig, Ralf

Background Multiple functional genomics data for complex human diseases have been published and made available by researchers worldwide. The main goal of these studies is the detailed analysis of a...

Gene expression variation in Down's syndrome mice allows prioritization of candidate genes (2007)

Sultan, Marc, Piccini, Ilaria, Balzereit, Daniela, Herwig, Ralf, Saran, Nidhi G, Lehrach, Hans, ...

Abstract Background Down's syndrome (DS), or trisomy 21, is a complex developmental disorder that exhibits many clinical signs that vary in occurrence and severity among patients. The molecular...

A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks (2007)

Poustka, Albert J, Kühn, Alexander, Groth, Detlef, Weise, Vesna, Yaguchi, Shunsuke, Burke, Robert D, ...

Abstract Background The genome of the sea urchin Strongylocentrotus purpuratus has recently been sequenced because it is a major model system for the study of gene regulatory networks. Embryonic...

Conserved molecular portraits of bovine and human blastocysts as a consequence of the transition from maternal to embryonic control of gene expression (2007)

Adjaye, James, Herwig, Ralf, Brink, Thore C., Herrmann, Doris, Greber, Boris, Sudheer, Smita, ...

The present study investigated mRNA expression profiles of bovine oocytes and blastocysts by using a cross-species hybridization approach employing an array consisting of 15,529 human cDNAs as probe,...

Nodal signaling activates differentiation genes during zebrafish gastrulation (2007)

Bennett, James D., Joubin, Katherine, Cheng, Simon, Aanstad, Pia, Herwig, Ralf, Clark, Matthew, ...

Nodal signals induce mesodermal and endodermal progenitors during vertebrate development. To determine the role of Nodal signaling at a genomic level, we isolated Nodal-regulated genes by expression...

Oligonucleotide fingerprinting of arrayed genomic DNA sequences using LNA-modified hybridization probes (2007)

Liu, Jian-Ping, Drungowski, Mario, Nyarsik, Lajos, Schwartz, Regine, Lehrach, Hans, Herwig, Ralf, ...

Recently, we established a robust method for the detection of hybridization events using a DNA microarray deposited on a nanoporous membrane. Here, in a follow-up study, we demonstrate the...

A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks (2007)

Poustka, Albert J., Kühn, Alexander, Groth, Detlef, Weise, Vesna, Yaguchi, Shunsuke, Burke, Robert D., ...

Background The genome of the sea urchin Strongylocentrotus purpuratus has recently been sequenced because it is a major model system for the study of gene regulatory networks. Embryonic expression...

Gene expression variation in Down's syndrome mice allows prioritization of candidate genes (2007)

Sultan, Marc, Piccini, Ilaria, Balzereit, Daniela, Herwig, Ralf, Saran, Nidhi G., Lehrach, Hans, ...

Background Down's syndrome (DS), or trisomy 21, is a complex developmental disorder that exhibits many clinical signs that vary in occurrence and severity among patients. The molecular mechanisms...

Resources, standards and tools for systems biology (2007)

Wierling, Christoph, Herwig, Ralf, Lehrach, Hans

Modelling and simulation techniques are valuable tools for the understanding of complex biological systems. The design of a computer model necessarily has many diverse inputs, such as information on...

Resources, standards and tools for systems biology (2007)

Wierling, Christoph, Herwig, Ralf, Lehrach, Hans

Modelling and simulation techniques are valuable tools for the understanding of complex biological systems. The design of a computer model necessarily has many diverse inputs, such as information on...

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence (2006)

Jann, Oliver C, Aerts, Jan, Jones, Michelle, Hastings, Nicola, Law, Andy, McKay, Stephanie, ...

Background: Several approaches can be used to determine the order of loci on chromosomes and hence develop maps of the genome. However, all mapping approaches are prone to errors either arising from...

Analysis of Oct4-dependent transcriptional networks regulating self-renewal and pluripotency in human embryonic stem cells (2006)

Babaie, Yasmin, Herwig, Ralf, Greber, Boris, Brink, Thore C., Wruck, Wasco, Groth, Detlef, ...

The POU domain transcription factor OCT4 is a key regulator of pluripotency in the early mammalian embryo and is highly expressed in the inner cell mass of the blastocyst. Consistent with its...

Dynamics of biological systems: role of systems biology in medical research (2006)

Assmus, Heike E., Herwig, Ralf, Cho, Kwang-Hyun, Wolkemhauer, Olaf

Cellular systems are networks of interacting components that change with time in response to external and internal events. Studying the dynamic behavior of these networks is the basis for an...

Expression profiling of drug response-from genes to pathways (2006)

Herwig, Ralf, Lehrach, Hans

Understanding individual response to a drug—what determines its efficacy and tolerability—is the major bottleneck in current drug development and clinical trials. Intracellular response and...

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence (2006)

Jann, Oliver C., Aerts, Jan, Jones, Michelle, Hastings, Nicola, Law, Andy, McKay, Stephanie, ...

Background Several approaches can be used to determine the order of loci on chromosomes and hence develop maps of the genome. However, all mapping approaches are prone to errors either arising from...

Age-specific hormonal decline is accompanied by transcriptional changes in human sebocytes in vitro (2006)

Makrantonaki, Evgenia, Adjaye, James, Herwig, Ralf, Brink, Thore C., Groth, Detlef, Hultschig, Claus, ...

The importance of hormones in endogenous aging has been displayed by recent studies performed on animal models and humans. To decipher the molecular mechanisms involved in aging we maintained human...

Primary differentiation in the human blastocyst : comparative molecular portraits of inner cell mass and trophectoderm cells (2005)

Adjaye, James, Huntriss, John, Herwig, Ralf, BenKahla, Alia, Brink, Thore C., Wierling, Christoph, ...

The primary differentiation event during mammalian development occurs at the blastocyst stage and leads to the delineation of the inner cell mass (ICM) and the trophectoderm (TE). We provide the...

Matrix Metalloproteinases of Epithelial Origin in Facial Sebum of Patients with Acne and their Regulation by Isotretinoin (2005)

Papakonstantinou, Eleni, Aletras, Alexios J., Glass, Evelyn, Tsogas, Panagiotis, Dionyssopoulos, Alexander, Adjaye, James, ...

Acne vulgaris is a skin disorder of the sebaceous follicles, involving hyperkeratinization and perifollicular inflammation. Matrix metalloproteinases (MMP) have a predominant role in inflammatory...

Transformation and other factors of the peptide mass spectrometry pairwise peak-list comparison process (2005)

Wolski, Witold E., Lalowski, Maciej, Martus, Peter, Herwig, Ralf, Giavalisco, Patrick, Gobom, Johan, ...

Background: Biological Mass Spectrometry is used to analyse peptides and proteins. A mass spectrum generates a list of measured mass to charge ratios and intensities of ionised peptides, which is...

Original Article (2005)

James Adjaye, John Huntriss, Ralf Herwig, Alia Benkahla, Thore Brink, Claus Hultschig, ...

The online version of this article, along with updated information and services, is located on the World Wide Web at:

Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo (2004)

Dickmeis, Thomas, Plessy, Charles, Rastegar, Sepand, Aanstad, Pia, Herwig, Ralf, Chalmel, Frédéric, ...

Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for...

Cross-species hybridisation of human and bovine orthologous genes on high density cDNA microarrays (2004)

Adjaye, James, Herwig, Ralf, Herrmann, Doris, Wruck, Wasco, BenKahla, Alia, Brink, Thore C., ...

Background Cross-species gene-expression comparison is a powerful tool for the discovery of evolutionarily conserved mechanisms and pathways of expression control. The usefulness of cDNA microarrays...

Multiplexed hybridizations of positively charge-tagged peptide nucleic acids detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (2004)

Bauer, Oliver, Guerasimova, Anna, Sauer, Sascha, Thamm, Sabine, Steinfath, Matthias, Herwig, Ralf, ...

Peptide nucleic acid (PNA) is a novel class of DNA analogues in which the entire sugar-phosphate backbone is replaced by a pseudopeptide counterpart. Owing to its neutral character and the consequent...

Transcript level alterations reflect gene dosage effects across multiple tissues in a mouse model of Down Syndrome (2004)

Kahlem, Pascal, Sultan, Marc, Herwig, Ralf, Steinfath, Matthias, Balzereit, Daniela, Eppens, Barbara, ...

Human trisomy 21, which results in Down syndrome (DS), is one of the most complicated congenital genetic anomalies compatible with life, yet little is known about the molecular basis of DS. It is...

Transcript Level Alterations Reflect Gene Dosage Effects Across Multiple Tissues in a Mouse Model of Down Syndrome (2004)

Kahlem, Pascal, Sultan, Marc, Herwig, Ralf, Steinfath, Matthias, Balzereit, Daniela, Eppens, Barbara, ...

Human trisomy 21, which results in Down syndrome (DS), is one of the most complicated congenital genetic anomalies compatible with life, yet little is known about the molecular basis of DS. It is...

Expression Profiling and Comparative Genomics Identify a Conserved Regulatory Region Controlling Midline Expression in the Zebrafish Embryo (2004)

Dickmeis, Thomas, Plessy, Charles, Rastegar, Sepand, Aanstad, Pia, Herwig, Ralf, Chalmel, Frédéric, ...

Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for...

Expression Profiling and Comparative Genomics Identify a Conserved Regulatory Region Controlling Midline Expression in the Zebrafish Embryo (2004)

Dickmeis, Thomas, Plessy, Charles, Rastegar, Sepand, Aanstad, Pia, Herwig, Ralf, Chalmel, Frédéric, ...

Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for...

Generation, annotation, evolutionary analysis, and database integration of 20,000 unique sea urchin EST clusters (2003)

Poustka, Albert J., Groth, Detlef, Hennig, Steffen, Thamm, Sabine, Cameron, Andrew, Beck, Alfred, ...

Together with the hemichordates, sea urchins represent basal groups of nonchordate invertebrate deuterostomes that occupy a key position in bilaterian evolution. Because sea urchin embryos are also...

New evidence for genome-wide duplications at the origin of vertebrates using an amphioxus gene set and completed animal genomes (2003)

Panopoulou, Georgia, Hennig, Steffen, Groth, Detlef, Krause, Antje, Poustka, Albert J., Herwig, Ralf, ...

The 2R hypothesis predicting two genome duplications at the origin of vertebrates is highly controversial. Studies published so far include limited sequence data from organisms close to the...

Exploring potential target genes of signaling pathways by predicting conserved transcription factor binding sites (2003)

Dieterich, Christoph, Herwig, Ralf, Vingron, Martin

Many cellular signaling pathways induce gene expression by activating specific transcription factor complexes. Conventional approaches to the prediction of transcription factor binding sites lead to...

New Evidence for Genome-Wide Duplications at the Origin of Vertebrates Using an Amphioxus Gene Set and Completed Animal Genomes (2003)

Panopoulou, Georgia, Hennig, Steffen, Groth, Detlef, Krause, Antje, Poustka, Albert J., Herwig, Ralf, ...

The 2R hypothesis predicting two genome duplications at the origin of vertebrates is highly controversial. Studies published so far include limited sequence data from organisms close to the...

Generation, Annotation, Evolutionary Analysis, and Database Integration of 20,000 Unique Sea Urchin EST Clusters (2003)

Poustka, Albert J., Groth, Detlef, Hennig, Steffen, Thamm, Sabine, Cameron, Andrew, Beck, Alfred, ...

Together with the hemichordates, sea urchins represent basal groups of nonchordate invertebrate deuterostomes that occupy a key position in bilaterian evolution. Because sea urchin embryos are also...

A gene expression map of human chromosome 21 orthologues in the mouse (2002)

Gitton, Yorick, Dahmane, Nadia, Baik, Sonya, I Altaba, Ariel Ruiz, Neidhardt, Lorenz, Scholze, Manuela, ...

The DNA sequence of human chromosome 21 (HSA21) has opened the route for a systematic molecular characterization of all of its genes. Trisomy 21 is associated with Down's syndrome, the most common...

Simulation of DNA array hybridization experiments and evaluation of critical parameters during subsequent image and data analysis (2002)

Wierling, Christoph K., Steinfath, Matthias, Elge, Thorsten, Schulze-Kremer, Steffen, Aanstad, Pia, Clark, Matthew, ...

Background: Gene expression analyses based on complex hybridization measurements have increased rapidly in recent years and have given rise to a huge amount of bioinformatic tools such as image...

Construction of a 'unigene' cDNA clone set by oligonucleotide fingerprinting allows access to 25 000 potential sugar beet genes (2002)

Herwig, Ralf, Schulz, Britta, Weisshaar, Bernd, Hennig, Steffen, Steinfath, Matthias, Drungowski, Mario, ...

Access to the complete gene inventory of an organism is crucial to understanding physiological processes like development, differentiation, pathogenesis, or adaptation to the environment. Transcripts...

DEFOG: A Practical Scheme for Deciphering Families of Genes (2002)

Fuchs, Tania, Malecova, Barbora, Linhart, Chaim, Sharan, Roded, Khen, Miriam, Herwig, Ralf, ...

We developed a novel efficient scheme, DEFOG (for "deciphering families of genes"), for determining sequences of numerous genes from a family of interest. The scheme provides a powerful means to...

Datenanalyse von Biochips: Von der Sequenz zum System (2002)

Herwig, Ralf, Schuchhardt, Johannes, Eickhoff, Holger, Herzel, Hanspeter, Lehrach, Hans

Die Zunahme unserer Kenntnisse über die molekularen Mechanismen u.a. von Wachstums-, Entwicklungs- und Differenzierungsprozessen hat die Grundlagen der molekularen Medizin innerhalb weniger Jahre...

Oligonucleotide fingerprinting including differental cDNA library screning (2002)

Bauer, Oliver, Janitz, Michal, Guerasimova, Anna, Herwig, Ralf, Lehrach, Hans, Radelof, Uwe

This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your...

Clustering gene expression profiles (2002)

Herwig, Ralf, Lehrach, Hans

This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your...

Information theoretical probe selection for hybridisation experiments (2000)

Herwig, Ralf, Schmitt, Armin O., Steinfath, Matthias, O’Brien, John, Seidel, Henrik, Meier-Ewert, Sebastian, ...

Motivation: The choice of probes is an important feature of hybridisation experiments. In this paper we present an algorithm that optimises probes with respect to a training set of sequences based on...

Statistical evaluation of differential expression on cDNA nylon arrays with replicated experiments

Herwig, Ralf, Aanstad, Pia, Clark, Matthew, Lehrach, Hans

In this paper we focus on the detection of differentially expressed genes according to changes in hybridization signals using statistical tests. These tests were applied to 14 208 zebrafish cDNA...

Large-Scale Clustering of cDNA-Fingerprinting Data

Herwig, Ralf, Poustka, Albert J., Müller, Christine, Bull, Christof, Lehrach, Hans, O'Brien, John

Clustering is one of the main mathematical challenges in large-scale gene expression analysis. We describe a clustering procedure based on a sequential k-means algorithm with additional refinements...

An Oligonucleotide Fingerprint Normalized and Expressed Sequence Tag Characterized Zebrafish cDNA Library

Clark, Matthew D., Hennig, Steffen, Herwig, Ralf, Clifton, Sandy W., Marra, Marco A., Lehrach, Hans, ...

The zebrafish is a powerful system for understanding the vertebrate genome, allowing the combination of genetic, molecular, and embryological analysis. Expressed sequence tags (ESTs) provide a rapid...

Expression Profiling and Comparative Genomics Identify a Conserved Regulatory Region Controlling Midline Expression in the Zebrafish Embryo

Dickmeis, Thomas, Plessy, Charles, Rastegar, Sepand, Aanstad, Pia, Herwig, Ralf, Chalmel, Frédéric, ...

Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for...

New Evidence for Genome-Wide Duplications at the Origin of Vertebrates Using an Amphioxus Gene Set and Completed Animal Genomes

Panopoulou, Georgia, Hennig, Steffen, Groth, Detlef, Krause, Antje, Poustka, Albert J., Herwig, Ralf, ...

The 2R hypothesis predicting two genome duplications at the origin of vertebrates is highly controversial. Studies published so far include limited sequence data from organisms close to the...

Generation, Annotation, Evolutionary Analysis, and Database Integration of 20,000 Unique Sea Urchin EST Clusters

Poustka, Albert J., Groth, Detlef, Hennig, Steffen, Thamm, Sabine, Cameron, Andrew, Beck, Alfred, ...

Together with the hemichordates, sea urchins represent basal groups of nonchordate invertebrate deuterostomes that occupy a key position in bilaterian evolution. Because sea urchin embryos are also...

Transcript Level Alterations Reflect Gene Dosage Effects Across Multiple Tissues in a Mouse Model of Down Syndrome

Kahlem, Pascal, Sultan, Marc, Herwig, Ralf, Steinfath, Matthias, Balzereit, Daniela, Eppens, Barbara, ...

Human trisomy 21, which results in Down syndrome (DS), is one of the most complicated congenital genetic anomalies compatible with life, yet little is known about the molecular basis of DS. It is...

A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks

Poustka, Albert J, Kühn, Alexander, Groth, Detlef, Weise, Vesna, Yaguchi, Shunsuke, Burke, Robert D, ...

Novel territory-specific markers from the sea urchin Strongylocentrotus purpuratus have been identified using screens for genes that are differentially expressed in lithium-treated embryos, which...

Gene expression variation in Down's syndrome mice allows prioritization of candidate genes

Sultan, Marc, Piccini, Ilaria, Balzereit, Daniela, Herwig, Ralf, Saran, Nidhi G, Lehrach, Hans, ...

RNA from eight Ts65Dn mice (a model of Down syndrome) and eight euploid mice were analysed by real-time PCR to examine inter-individual gene expression levels as a function of trisomy.

ConsensusPathDB—a database for integrating human functional interaction networks

Kamburov, Atanas, Wierling, Christoph, Lehrach, Hans, Herwig, Ralf

ConsensusPathDB is a database system for the integration of human functional interactions. Current knowledge of these interactions is dispersed in more than 200 databases, each having a specific...

GeNGe: systematic generation of gene regulatory networks

Hache, Hendrik, Wierling, Christoph, Lehrach, Hans, Herwig, Ralf

Summary: The analysis of gene regulatory networks (GRNs) is a central goal of bioinformatics highly accelerated by the advent of new experimental techniques, such as RNA interference. A battery of...

Reverse Engineering of Gene Regulatory Networks: A Comparative Study

Hache, Hendrik, Lehrach, Hans, Herwig, Ralf

Reverse engineering of gene regulatory networks has been an intensively studied topic in bioinformatics since it constitutes an intermediate step from explorative to causative gene expression...