Structural studies of West Nile virus in complex with neutralizing antibodies (2008)
Kuhn, Richard J, Kaufmann, Bärbel, Lok, Shee, Cherrier, Mick, Perera, Rushika, Sedlak, Dagmar, ...
No abstract available.
Sindbis virus nucleocapsid assembly: RNA folding promotes capsid protein dimerization (2004)
LINGER, BENJAMIN R., KUNOVSKA, LYUDMYLA, KUHN, RICHARD J., GOLDEN, BARBARA L.
In Sindbis virus, initiation of nucleocapsid core assembly begins with recognition of the encapsidation signal of the viral RNA genome by capsid protein. This nucleation event drives the recruitment...
Choi, Kyung H., Groarke, James M., Young, Dorothy C., Rossmann, Michael G., Pevear, Daniel C., Kuhn, Richard J., ...
Bovine viral diarrhea virus (BVDV) nonstructural protein 5B is an RNA-dependent RNA polymerase, essential for viral replication. Initial attempts to crystallize a soluble form of the 695-residue BVDV...
Elton L. Menon, Rushika Perera, Richard J. Kuhn, Harry Morrison
We have extended our study of the decomposition of urocanic acid (UCA) with ultraviolet A radiation (UV-A) by the self-sensitized generation of singlet oxygen (see Photochem. Photobiol. 75, 565...
Zhang, Wei, Fisher, Bonnie R., Olson, Norman H., Strauss, James H., Kuhn, Richard J., Baker, Timothy S.
Aura and Sindbis viruses are closely related alphaviruses. Unlike other alphaviruses, Aura virus efficiently encapsidates both genomic RNA (11.8 kb) and subgenomic RNA (4.2 kb) to form virus...
Structure of decay-accelerating factor bound to echovirus 7: a virus-receptor complex. (2002)
He, Yongning, Lin, Feng, Chipman, Paul R., Bator, Carol M., Baker, Timothy S., Shoham, Menachem, ...
Echoviruses are enteroviruses that belong to Picornaviridae. Many echoviruses use decay-accelerating factor (DAF) as their cellular receptor. DAF is a glycosylphosphatidyl inositol-anchored...
Heil, Marintha L., Albee, Alison, Strauss, James H., Kuhn, Richard J.
Passage of Ross River virus strain NB5092 in avian cells has been previously shown to select for virus variants that have enhanced replication in these cells. Sequencing of these variants identified...
Photobiological Properties of Positively Charged Methylene Violet Analogs (2000)
Melissa A. Houghtaling, Rushika Perera, Katherine E. Owen, Stephen Wagner, Richard J. Kuhn, Harry Morrison
O-Methyl methylene violet (OMeMV), O-methyl bromomethylene violet (OMeBrMV) and O-methyl iodomethylene violet (OMeIMV) have been prepared in order to test their potential utility as anti-viral and...
Nucleocapsid-glycoprotein interactions required for assembly of alphaviruses (1994)
López, Susana, Yao, Jian-Sheng, Kuhn, Richard J., Strauss, Ellen G., Strauss, James H.
We have studied interactions between nucleocapsids and glycoproteins required for budding of alphaviruses, using Ross River virus-Sindbis virus chimeras in which the nucleocapsid protein is derived...
Kuhn, Richard J., Griffin, Diane E., Zhang, Hong, Niesters, Hubert G. M., Strauss, James H.
We examined a panel of Sindbis virus mutants containing defined mutations in the 5' nontranslated region of the genome RNA, in the 3' nontranslated region, or in both for their growth in cultured...
Reuer, Quentin, Kuhn, Richard J., Wimmer, Eckard
Viral RNA synthesis was assayed in HeLa cells transfected with nonviable poliovirus RNA mutated in the genome-linked protein VPg-coding region. The transfecting RNA was transcribed in vitro from...
Mutagenesis of the 3' nontranslated region of Sindbis virus RNA (1990)
Kuhn, Richard J., Hong, Zhang, Strauss, James H.
A cDNA clone from which infectious RNA can be transcribed was used to construct 42 site-specific mutations in the 3' nontranslated region of the Sindbis virus genome. The majority of these mutations...
Murray, Michael G., Kuhn, Richard J., Arita, Mineo, Kawamura, Noriyuki, Nomoto, Akio, Wimmer, Eckard
Poliovirus exists as three stable serotypes (PV-1, PV-2, and PV-3). These viruses display three antigenic sites each, designated N-AgI, N-AgII, and N-AgIII. When mice are immunized with poliovirus,...
Kuhn, Richard J., Tada, Hiroomi, Ypma-Wong, Mary Frances, Dunn, John J., Semler, Bert L., Wimmer, Eckard
By following a strategy of genetic analysis of poliovirus, we have constructed a synthetic "mutagenesis cartridge" spanning the genome-linked viral protein coding region and flanking cleavage sites...
Interaction of the poliovirus receptor with poliovirus
He, Yongning, Bowman, Valorie D., Mueller, Steffen, Bator, Carol M., Bella, Jordi, Peng, Xiaozhong, ...
The structure of the extracellular, three-domain poliovirus receptor (CD155) complexed with poliovirus (serotype 1) has been determined to 22-Å resolution by means of cryo-electron microscopy and...
Nucleic Acid-Dependent Cross-Linking of the Nucleocapsid Protein of Sindbis Virus
Tellinghuisen, Timothy L., Kuhn, Richard J.
The assembly of the alphavirus nucleocapsid core is a multistep event requiring the association of the nucleocapsid protein with nucleic acid and the subsequent oligomerization of capsid proteins...
In Vitro Assembly of Alphavirus Cores by Using Nucleocapsid Protein Expressed in Escherichia coli
Tellinghuisen, Timothy L., Hamburger, Agnes E., Fisher, Bonnie R., Ostendorp, Ralf, Kuhn, Richard J.
The production of the alphavirus virion is a multistep event requiring the assembly of the nucleocapsid core in the cytoplasm and the maturation of the glycoproteins in the endoplasmic reticulum and...
Alphavirus Nucleocapsid Protein Contains a Putative Coiled Coil α-Helix Important for Core Assembly
Perera, Rushika, Owen, Katherine E., Tellinghuisen, Timothy L., Gorbalenya, Alexander E., Kuhn, Richard J.
The alphavirus nucleocapsid core is formed through the energetic contributions of multiple noncovalent interactions mediated by the capsid protein. This protein consists of a poorly conserved...
Heil, Marintha L., Albee, Alison, Strauss, James H., Kuhn, Richard J.
Passage of Ross River virus strain NB5092 in avian cells has been previously shown to select for virus variants that have enhanced replication in these cells. Sequencing of these variants identified...
Interaction of Coxsackievirus A21 with Its Cellular Receptor, ICAM-1
Xiao, Chuan, Bator, Carol M., Bowman, Valorie D., Rieder, Elizabeth, He, Yongning, Hébert, Benoît, ...
Coxsackievirus A21 (CAV21), like human rhinoviruses (HRVs), is a causative agent of the common cold. It uses the same cellular receptor, intercellular adhesion molecule 1 (ICAM-1), as does the major...
Ross River Virus Glycoprotein-Pseudotyped Retroviruses and Stable Cell Lines for Their Production
Sharkey, C. Matthew, North, Cynthia L., Kuhn, Richard J., Sanders, David Avram
Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus...
Tellinghuisen, Timothy L., Perera, Rushika, Kuhn, Richard J.
A nucleic acid-bound capsid protein dimer was previously identified using a Sindbis virus in vitro nucleocapsid assembly system and cross-linking reagents. Cross-link mapping, in combination with a...
Structure of decay-accelerating factor bound to echovirus 7: A virus-receptor complex
He, Yongning, Lin, Feng, Chipman, Paul R., Bator, Carol M., Baker, Timothy S., Shoham, Menachem, ...
Echoviruses are enteroviruses that belong to Picornaviridae. Many echoviruses use decay-accelerating factor (DAF) as their cellular receptor. DAF is a glycosylphosphatidyl inositol-anchored...
Zhang, Wei, Fisher, Bonnie R., Olson, Norman H., Strauss, James H., Kuhn, Richard J., Baker, Timothy S.
Aura and Sindbis viruses are closely related alphaviruses. Unlike other alphaviruses, Aura virus efficiently encapsidates both genomic RNA (11.8 kb) and subgenomic RNA (4.2 kb) to form virus...
Mukhopadhyay, Suchetana, Chipman, Paul R., Hong, Eunmee M., Kuhn, Richard J., Rossmann, Michael G.
In vitro-assembled core-like particles produced from alphavirus capsid protein and nucleic acid were studied by cryoelectron microscopy. These particles were found to have a diameter of 420 Å with...
Placement of the Structural Proteins in Sindbis Virus
Zhang, Wei, Mukhopadhyay, Suchetana, Pletnev, Sergei V., Baker, Timothy S., Kuhn, Richard J., Rossmann, Michael G.
The structure of the lipid-enveloped Sindbis virus has been determined by fitting atomic resolution crystallographic structures of component proteins into an 11-Å resolution cryoelectron microscopy...
Complexes of Poliovirus Serotypes with Their Common Cellular Receptor, CD155
He, Yongning, Mueller, Steffen, Chipman, Paul R., Bator, Carol M., Peng, Xiaozhong, Bowman, Valorie D., ...
Structures of all three poliovirus (PV) serotypes (PV1, PV2, and PV3) complexed with their cellular receptor, PV receptor (PVR or CD155), were determined by cryoelectron microscopy. Both glycosylated...
Flavivirus Capsid Is a Dimeric Alpha-Helical Protein
Jones, Christopher T., Ma, Lixin, Burgner, John W., Groesch, Teresa D., Post, Carol B., Kuhn, Richard J.
The capsid proteins of two flaviviruses, yellow fever virus and dengue virus, were expressed in Escherichia coli and purified to near homogeneity suitable for biochemical characterization and...
Structures of immature flavivirus particles
Zhang, Ying, Corver, Jeroen, Chipman, Paul R., Zhang, Wei, Pletnev, Sergei V., Sedlak, Dagmar, ...
Structures of prM-containing dengue and yellow fever virus particles were determined to 16 and 25 Å resolution, respectively, by cryoelectron microscopy and image reconstruction techniques. The...
A Heterologous Coiled Coil Can Substitute for Helix I of the Sindbis Virus Capsid Protein
Perera, Rushika, Navaratnarajah, Chanakha, Kuhn, Richard J.
Alphavirus core assembly proceeds along an assembly pathway involving a dimeric assembly intermediate. Several regions of the alphavirus capsid protein have been implicated in promoting and...
Solution structure of dengue virus capsid protein reveals another fold
Ma, Lixin, Jones, Christopher T., Groesch, Teresa D., Kuhn, Richard J., Post, Carol Beth
Dengue virus is responsible for ≈50–100 million infections, resulting in nearly 24,000 deaths annually. The capsid (C) protein of dengue virus is essential for specific encapsidation of the RNA...
Choi, Kyung H., Groarke, James M., Young, Dorothy C., Kuhn, Richard J., Smith, Janet L., Pevear, Daniel C., ...
The bovine viral diarrhea virus (BVDV) RNA-dependent RNA polymerase can initiate RNA replication by a de novo mechanism without a primer. The structure of BVDV polymerase, determined to 2.9-Å...
Wu, Jinhua, Bera, Aloke Kumar, Kuhn, Richard J., Smith, Janet L.
Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus...
Sindbis virus nucleocapsid assembly: RNA folding promotes capsid protein dimerization
LINGER, BENJAMIN R., KUNOVSKA, LYUDMYLA, KUHN, RICHARD J., GOLDEN, BARBARA L.
In Sindbis virus, initiation of nucleocapsid core assembly begins with recognition of the encapsidation signal of the viral RNA genome by capsid protein. This nucleation event drives the recruitment...
Interaction of the poliovirus receptor with poliovirus
He, Yongning, Bowman, Valorie D., Mueller, Steffen, Bator, Carol M., Bella, Jordi, Peng, Xiaozhong, ...
The structure of the extracellular, three-domain poliovirus receptor (CD155) complexed with poliovirus (serotype 1) has been determined to 22-Å resolution by means of cryo-electron microscopy and...
Nucleic Acid-Dependent Cross-Linking of the Nucleocapsid Protein of Sindbis Virus
Tellinghuisen, Timothy L., Kuhn, Richard J.
The assembly of the alphavirus nucleocapsid core is a multistep event requiring the association of the nucleocapsid protein with nucleic acid and the subsequent oligomerization of capsid proteins...
In Vitro Assembly of Alphavirus Cores by Using Nucleocapsid Protein Expressed in Escherichia coli
Tellinghuisen, Timothy L., Hamburger, Agnes E., Fisher, Bonnie R., Ostendorp, Ralf, Kuhn, Richard J.
The production of the alphavirus virion is a multistep event requiring the assembly of the nucleocapsid core in the cytoplasm and the maturation of the glycoproteins in the endoplasmic reticulum and...
Alphavirus Nucleocapsid Protein Contains a Putative Coiled Coil α-Helix Important for Core Assembly
Perera, Rushika, Owen, Katherine E., Tellinghuisen, Timothy L., Gorbalenya, Alexander E., Kuhn, Richard J.
The alphavirus nucleocapsid core is formed through the energetic contributions of multiple noncovalent interactions mediated by the capsid protein. This protein consists of a poorly conserved...
Heil, Marintha L., Albee, Alison, Strauss, James H., Kuhn, Richard J.
Passage of Ross River virus strain NB5092 in avian cells has been previously shown to select for virus variants that have enhanced replication in these cells. Sequencing of these variants identified...
Interaction of Coxsackievirus A21 with Its Cellular Receptor, ICAM-1
Xiao, Chuan, Bator, Carol M., Bowman, Valorie D., Rieder, Elizabeth, He, Yongning, Hébert, Benoît, ...
Coxsackievirus A21 (CAV21), like human rhinoviruses (HRVs), is a causative agent of the common cold. It uses the same cellular receptor, intercellular adhesion molecule 1 (ICAM-1), as does the major...
Ross River Virus Glycoprotein-Pseudotyped Retroviruses and Stable Cell Lines for Their Production
Sharkey, C. Matthew, North, Cynthia L., Kuhn, Richard J., Sanders, David Avram
Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus...
Tellinghuisen, Timothy L., Perera, Rushika, Kuhn, Richard J.
A nucleic acid-bound capsid protein dimer was previously identified using a Sindbis virus in vitro nucleocapsid assembly system and cross-linking reagents. Cross-link mapping, in combination with a...
Structure of decay-accelerating factor bound to echovirus 7: A virus-receptor complex
He, Yongning, Lin, Feng, Chipman, Paul R., Bator, Carol M., Baker, Timothy S., Shoham, Menachem, ...
Echoviruses are enteroviruses that belong to Picornaviridae. Many echoviruses use decay-accelerating factor (DAF) as their cellular receptor. DAF is a glycosylphosphatidyl inositol-anchored...
Zhang, Wei, Fisher, Bonnie R., Olson, Norman H., Strauss, James H., Kuhn, Richard J., Baker, Timothy S.
Aura and Sindbis viruses are closely related alphaviruses. Unlike other alphaviruses, Aura virus efficiently encapsidates both genomic RNA (11.8 kb) and subgenomic RNA (4.2 kb) to form virus...
Mukhopadhyay, Suchetana, Chipman, Paul R., Hong, Eunmee M., Kuhn, Richard J., Rossmann, Michael G.
In vitro-assembled core-like particles produced from alphavirus capsid protein and nucleic acid were studied by cryoelectron microscopy. These particles were found to have a diameter of 420 Å with...
Placement of the Structural Proteins in Sindbis Virus
Zhang, Wei, Mukhopadhyay, Suchetana, Pletnev, Sergei V., Baker, Timothy S., Kuhn, Richard J., Rossmann, Michael G.
The structure of the lipid-enveloped Sindbis virus has been determined by fitting atomic resolution crystallographic structures of component proteins into an 11-Å resolution cryoelectron microscopy...
Complexes of Poliovirus Serotypes with Their Common Cellular Receptor, CD155
He, Yongning, Mueller, Steffen, Chipman, Paul R., Bator, Carol M., Peng, Xiaozhong, Bowman, Valorie D., ...
Structures of all three poliovirus (PV) serotypes (PV1, PV2, and PV3) complexed with their cellular receptor, PV receptor (PVR or CD155), were determined by cryoelectron microscopy. Both glycosylated...
Flavivirus Capsid Is a Dimeric Alpha-Helical Protein
Jones, Christopher T., Ma, Lixin, Burgner, John W., Groesch, Teresa D., Post, Carol B., Kuhn, Richard J.
The capsid proteins of two flaviviruses, yellow fever virus and dengue virus, were expressed in Escherichia coli and purified to near homogeneity suitable for biochemical characterization and...
Structures of immature flavivirus particles
Zhang, Ying, Corver, Jeroen, Chipman, Paul R., Zhang, Wei, Pletnev, Sergei V., Sedlak, Dagmar, ...
Structures of prM-containing dengue and yellow fever virus particles were determined to 16 and 25 Å resolution, respectively, by cryoelectron microscopy and image reconstruction techniques. The...
A Heterologous Coiled Coil Can Substitute for Helix I of the Sindbis Virus Capsid Protein
Perera, Rushika, Navaratnarajah, Chanakha, Kuhn, Richard J.
Alphavirus core assembly proceeds along an assembly pathway involving a dimeric assembly intermediate. Several regions of the alphavirus capsid protein have been implicated in promoting and...
Solution structure of dengue virus capsid protein reveals another fold
Ma, Lixin, Jones, Christopher T., Groesch, Teresa D., Kuhn, Richard J., Post, Carol Beth
Dengue virus is responsible for ≈50–100 million infections, resulting in nearly 24,000 deaths annually. The capsid (C) protein of dengue virus is essential for specific encapsidation of the RNA...
Choi, Kyung H., Groarke, James M., Young, Dorothy C., Kuhn, Richard J., Smith, Janet L., Pevear, Daniel C., ...
The bovine viral diarrhea virus (BVDV) RNA-dependent RNA polymerase can initiate RNA replication by a de novo mechanism without a primer. The structure of BVDV polymerase, determined to 2.9-Å...
Wu, Jinhua, Bera, Aloke Kumar, Kuhn, Richard J., Smith, Janet L.
Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus...
Sindbis virus nucleocapsid assembly: RNA folding promotes capsid protein dimerization
LINGER, BENJAMIN R., KUNOVSKA, LYUDMYLA, KUHN, RICHARD J., GOLDEN, BARBARA L.
In Sindbis virus, initiation of nucleocapsid core assembly begins with recognition of the encapsidation signal of the viral RNA genome by capsid protein. This nucleation event drives the recruitment...
Alphavirus Capsid Protein Helix I Controls a Checkpoint in Nucleocapsid Core Assembly
Hong, Eunmee M., Perera, Rushika, Kuhn, Richard J.
The assembly of the alphavirus nucleocapsid core has been investigated using an in vitro assembly system. The C-terminal two-thirds of capsid protein (CP), residues 81 to 264 in Sindbis virus (SINV),...
Tomar, Shailly, Hardy, Richard W., Smith, Janet L., Kuhn, Richard J.
The RNA-dependent RNA polymerase nsP4 is an integral part of the alphavirus replication complex. To define the role of nsP4 in viral RNA replication and for a structure-function analysis, we...
West Nile virus in complex with the Fab fragment of a neutralizing monoclonal antibody
Kaufmann, Bärbel, Nybakken, Grant E., Chipman, Paul R., Zhang, Wei, Diamond, Michael S., Fremont, Daved H., ...
Flaviviruses, such as West Nile virus (WNV), are significant human pathogens. The humoral immune response plays an important role in the control of flavivirus infection and disease. The structure of...
Functional Requirements of the Yellow Fever Virus Capsid Protein▿
Patkar, Chinmay G., Jones, Christopher T., Chang, Yu-hsuan, Warrier, Ranjit, Kuhn, Richard J.
Although it is known that the flavivirus capsid protein is essential for genome packaging and formation of infectious particles, the minimal requirements of the dimeric capsid protein for virus...
Structure of Immature West Nile Virus▿
Zhang, Ying, Kaufmann, Bärbel, Chipman, Paul R., Kuhn, Richard J., Rossmann, Michael G.
The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by...
Van Der Schaar, Hilde M., Rust, Michael J., Waarts, Barry-Lee, Kuhn, Richard J., Wilschut, Jan, ...
In this study, we investigated the cell entry characteristics of dengue virus (DENV) type 2 strain S1 on mosquito, BHK-15, and BS-C-1 cells. The concentration of virus particles measured by...
Functional Requirements of the Yellow Fever Virus Capsid Protein
Patkar, Chinmay G., Jones, Christopher T., Chang, Yu-hsuan, Warrier, Ranjit, Kuhn, Richard J.
Choi, Kyung H., Groarke, James M., Young, Dorothy C., Rossmann, Michael G., Pevear, Daniel C., Kuhn, Richard J., ...
Bovine viral diarrhea virus (BVDV) nonstructural protein 5B is an RNA-dependent RNA polymerase, essential for viral replication. Initial attempts to crystallize a soluble form of the 695-residue BVDV...
Patkar, Chinmay G., Kuhn, Richard J.
In flaviviruses it has been proposed that there is a coupling between genome replication and virion assembly and that nonstructural proteins are involved in this process. It was previously reported...
Warrier, Ranjit, Linger, Benjamin R., Golden, Barbara L., Kuhn, Richard J.
Sindbis virus is an enveloped positive-sense RNA virus in the alphavirus genus. The nucleocapsid core contains the genomic RNA surrounded by 240 copies of a single capsid protein. The capsid protein...
Geders, Todd W., Smith, Janet L., Kuhn, Richard J.
The plus-strand RNA genome of Sindbis virus (SINV) encodes four nonstructural proteins (nsP1 to nsP4) that are involved in the replication of the viral RNA. The ∼800-amino-acid nsP2 consists of an...