Robert K. Neely

Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes (2009)

Neely, Robert K., Tamulaitis, Gintautas, Chen, Kai, Kubala, Marta, Siksnys, Virginijus, Jones, Anita C.

Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence...

The BsaHI restriction-modification system: Cloning, sequencing and analysis of conserved motifs (2008)

Neely, Robert K, Roberts, Richard J

Abstract Background Restriction and modification enzymes typically recognise short DNA sequences of between two and eight bases in length. Understanding the mechanism of this recognition represents a...

Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI-DNA complexes (2005)

Neely, Robert K., Daujotyte, Dalia, Grazulis, Saulius, Magennis, Steven W., Dryden, David T. F., Klimasauskas, Saulius, ...

DNA base flipping is an important mechanism in molecular enzymology, but its study is limited by the lack of an accessible and reliable diagnostic technique. A series of crystalline complexes of a...

Diatom succession in an urban reservoir system (1996)

Donar, Christopher M., Neely, Robert K., Stoermer, Eugene F.

A 43 cm by 5 cm diameter sediment core sample was obtained from Ford Lake reservoir in Washtenaw County, Michigan, and sectioned at 1 cm intervals. The purpose of this study was to determine whether...

New Spatially Explicit Method for Detecting Extracellular Protease Activity in Biofilms

Francoeur, Steven N., Wetzel, Robert G., Neely, Robert K.

A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin....

Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI–DNA complexes

Neely, Robert K., Daujotyte, Dalia, Grazulis, Saulius, Magennis, Steven W., Dryden, David T. F., Klimašauskas, Saulius, ...

DNA base flipping is an important mechanism in molecular enzymology, but its study is limited by the lack of an accessible and reliable diagnostic technique. A series of crystalline complexes of a...

New Spatially Explicit Method for Detecting Extracellular Protease Activity in Biofilms

Francoeur, Steven N., Wetzel, Robert G., Neely, Robert K.

A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin....

Time-resolved fluorescence of 2-aminopurine as a probe of base flipping in M.HhaI–DNA complexes

Neely, Robert K., Daujotyte, Dalia, Grazulis, Saulius, Magennis, Steven W., Dryden, David T. F., Klimašauskas, Saulius, ...

DNA base flipping is an important mechanism in molecular enzymology, but its study is limited by the lack of an accessible and reliable diagnostic technique. A series of crystalline complexes of a...

Application of the [3H]Leucine Incorporation Technique for Quantification of Bacterial Secondary Production Associated with Decaying Wetland Plant Litter

Gillies, Jane E., Kuehn, Kevin A., Francoeur, Steven N., Neely, Robert K.

The radiolabeled leucine incorporation technique for quantifying rates of bacterial production has increased in popularity since its original description for bacterioplankton communities. Prior...

Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes

Neely, Robert K., Tamulaitis, Gintautas, Chen, Kai, Kubala, Marta, Siksnys, Virginijus, Jones, Anita C.

Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence...