Miranda, Kildare, Rodrigues, Claudia O., Hentschel, Joachim, Vercesi, Anibal, Plattner, Helmut, Souza, Wanderley De, ...
Acidocalcisomes are acidic calcium storage compartments described initially in trypanosomatid and apicomplexan parasites, and recently found in other unicellular eukaryotes. The aim of this study was...
Dynamics of polymorphism of acidocalcisomes in Leishmania parasites (2004)
Miranda, Kildare, Docampo, Roberto, Grillo, Orlando, Franzen, Anderson, Attias, Márcia, Vercesi, Anibal, ...
Growth of Leishmania mexicana amazonensis promastigotes in different culture media resulted in structurally and chemically different acidocalcisomes. When grown in SDM-79 medium, the promastigotes...
The plasma membrane potential (ΔΨ) of procyclic and bloodstream trypomastigotes of Trypanosoma brucei was studied using the potentiometric fluorescent dye bisoxonol. Our results suggest that a...
CLAUDIA O. RODRIGUES, ROSANA CATISTI, SERGIO A. UYEMURA, ANIBAL E. VERCESI, RENEE LIRA, CARLOS RODRIGUEZ, ...
Respiration, oxidative phosphorylation, and the corresponding changes in membrane potential (ΔΨ) of Trypanosoma cruzi epimastigotes grown either in liver infusion-tryptose (LIT) or brain heart...
Rodrigues, Claudia O., Scott, David A., Docampo, Roberto
Inorganic pyrophosphate promoted the acidification of an intracellular compartment in permeabilized procyclic trypomastigotes of Trypanosoma brucei, as measured by acridine orange uptake. The proton...
Lu, Hong-Gang, Zhong, Li, De Souza, Wanderley, Benchimol, Marlene, Moreno, Silvia, Docampo, Roberto
The survival of a eukaryotic protozoan as an obligate parasite in the interior of a eukaryotic host cell implies its adaptation to an environment with a very different ionic composition from that of...
Yardley, Vanessa, Khan, Anis A., Martin, Michael B., Slifer, Teri R., Araujo, Fausto G., Moreno, Silvia N. J., ...
The in vivo activities of three bisphosphonates were determined against Leishmania donovani and Toxoplasma gondii. Alendronate was essentially inactive against both parasites. Pamidronate was active...
Urbina, Julio A., Concepcion, Juan Luis, Montalvetti, Andrea, Rodriguez, Juan B., Docampo, Roberto
We investigated the molecular basis of the activity of 4-phenoxyphenoxyethyl thiocyanate (WC-9) against Trypanosoma cruzi, the etiological agent of Chagas’ disease. We found that growth inhibition...
Salto, Maria Laura, Bertello, Laura E., Vieira, Mauricio, Docampo, Roberto, Moreno, Silvia N. J., De Lederkremer, Rosa M.
Differentiation of Trypanosoma cruzi trypomastigotes to amastigotes inside myoblasts or in vitro, at low extracellular pH, in the presence of [3H]palmitic acid or [3H]inositol revealed differential...
The Interplay between Folding-facilitating Mechanisms in Trypanosoma cruzi Endoplasmic Reticulum
Conte, Ianina, Labriola, Carlos, Cazzulo, Juan J., Docampo, Roberto, Parodi, Armando J.
Lectin (calreticulin [CRT])-N-glycan–mediated quality control of glycoprotein folding is operative in trypanosomatid protozoa but protein-linked monoglucosylated N-glycans are exclusively formed in...
Generation of Superoxide Anions and Hydrogen Peroxide from β-Lapachone in Bacteria
Cruz, Fernando S., Docampo, Roberto, Boveris, Alberto
β-Lapachone markedly increased the generation of superoxide anions and hydrogen peroxide by subcellular membranes of Bacillus subtilis and Bacillus stearothermophilus. Peroxide generation by...
Characterization of Farnesylated Protein Tyrosine Phosphatase TcPRL-1 from Trypanosoma cruzi†
Cuevas, Ileana C., Rohloff, Peter, Sánchez, Daniel O., Docampo, Roberto
Protein tyrosine kinases and phosphatases play important roles in the regulation of cell growth, development, and differentiation. We report here the identification in Trypanosoma cruzi of a gene...
Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruzi
Vieira, Mauricio, Rohloff, Peter, Luo, Shuhong, De Souza, Wanderley, Docampo, Roberto
Previous studies in Trypanosoma cruzi, the etiologic agent of Chagas disease, have resulted in the cloning and sequencing of a pair of tandemly linked genes (TcHA1 and TcHA2) that encode P...
Polyphosphate modulates blood coagulation and fibrinolysis
Smith, Stephanie A., Mutch, Nicola J., Baskar, Deepak, Rohloff, Peter, Docampo, Roberto, Morrissey, James H.
Inorganic polyphosphate is an abundant component of acidocalcisomes of bacteria and unicellular eukaryotes. Human platelet dense granules strongly resemble acidocalcisomes, and we recently showed...
Rodrigues, Claudia O., Scott, David A., Docampo, Roberto
Inorganic pyrophosphate promoted the acidification of an intracellular compartment in permeabilized procyclic trypomastigotes of Trypanosoma brucei, as measured by acridine orange uptake. The proton...
Lu, Hong-Gang, Zhong, Li, De Souza, Wanderley, Benchimol, Marlene, Moreno, Silvia, Docampo, Roberto
The survival of a eukaryotic protozoan as an obligate parasite in the interior of a eukaryotic host cell implies its adaptation to an environment with a very different ionic composition from that of...
Yardley, Vanessa, Khan, Anis A., Martin, Michael B., Slifer, Teri R., Araujo, Fausto G., Moreno, Silvia N. J., ...
The in vivo activities of three bisphosphonates were determined against Leishmania donovani and Toxoplasma gondii. Alendronate was essentially inactive against both parasites. Pamidronate was active...
Urbina, Julio A., Concepcion, Juan Luis, Montalvetti, Andrea, Rodriguez, Juan B., Docampo, Roberto
We investigated the molecular basis of the activity of 4-phenoxyphenoxyethyl thiocyanate (WC-9) against Trypanosoma cruzi, the etiological agent of Chagas’ disease. We found that growth inhibition...
Salto, Maria Laura, Bertello, Laura E., Vieira, Mauricio, Docampo, Roberto, Moreno, Silvia N. J., De Lederkremer, Rosa M.
Differentiation of Trypanosoma cruzi trypomastigotes to amastigotes inside myoblasts or in vitro, at low extracellular pH, in the presence of [3H]palmitic acid or [3H]inositol revealed differential...
The Interplay between Folding-facilitating Mechanisms in Trypanosoma cruzi Endoplasmic Reticulum
Conte, Ianina, Labriola, Carlos, Cazzulo, Juan J., Docampo, Roberto, Parodi, Armando J.
Lectin (calreticulin [CRT])-N-glycan–mediated quality control of glycoprotein folding is operative in trypanosomatid protozoa but protein-linked monoglucosylated N-glycans are exclusively formed in...
Generation of Superoxide Anions and Hydrogen Peroxide from β-Lapachone in Bacteria
Cruz, Fernando S., Docampo, Roberto, Boveris, Alberto
β-Lapachone markedly increased the generation of superoxide anions and hydrogen peroxide by subcellular membranes of Bacillus subtilis and Bacillus stearothermophilus. Peroxide generation by...
Characterization of Farnesylated Protein Tyrosine Phosphatase TcPRL-1 from Trypanosoma cruzi†
Cuevas, Ileana C., Rohloff, Peter, Sánchez, Daniel O., Docampo, Roberto
Protein tyrosine kinases and phosphatases play important roles in the regulation of cell growth, development, and differentiation. We report here the identification in Trypanosoma cruzi of a gene...
Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruzi
Vieira, Mauricio, Rohloff, Peter, Luo, Shuhong, De Souza, Wanderley, Docampo, Roberto
Previous studies in Trypanosoma cruzi, the etiologic agent of Chagas disease, have resulted in the cloning and sequencing of a pair of tandemly linked genes (TcHA1 and TcHA2) that encode P...
Polyphosphate modulates blood coagulation and fibrinolysis
Smith, Stephanie A., Mutch, Nicola J., Baskar, Deepak, Rohloff, Peter, Docampo, Roberto, Morrissey, James H.
Inorganic polyphosphate is an abundant component of acidocalcisomes of bacteria and unicellular eukaryotes. Human platelet dense granules strongly resemble acidocalcisomes, and we recently showed...
Fang, Jianmin, Rohloff, Peter, Miranda, Kildare, Docampo, Roberto
Inorganic poly P (polyphosphate) is an abundant component of acidocalcisomes of Trypanosoma brucei. In the present study we report the presence of a protein homologous with the yeast Vtc1p (vacuolar...
Fifth Annual Conference on New and Re-Emerging Infectious Diseases
The fifth annual Conference on New and Re-Emerging Infectious Diseases was hosted on April 18–19, 2002, by the College of Veterinary Medicine, University of Illinois at Urbana-Champaign (UIUC). The...
Schoijet, Alejandra C., Miranda, Kildare, Girard-Dias, Wendell, De Souza, Wanderley, Flawiá, Mirtha M., Torres, Héctor N., ...
Trypanosoma cruzi, the etiological agent of Chagas disease, has the ability to respond to a variety of environmental changes during its life cycle both in the insect vector and in the vertebrate...