S. Zeuzem

Second-line chemotherapy with pemetrexed after gemcitabine failure in patients with advanced pancreatic cancer: a multicenter phase II trial (2007)

Boeck, S, Weigang-Köhler, K, Fuchs, M, Kettner, E, Quietzsch, D, Trojan, J, ...

Background: A standard second-line chemotherapy regimen has yet to be defined for patients with gemcitabine (Gem)-refractory advanced pancreatic cancer (PC). Patients and methods: In this multicenter...

Should treatment with peginterferon plus ribavirin be intensified in patients with HCV genotype 2/3 without a rapid virological response? (2007)

Willems, B, Hadziyannis, S. J, Morgan, T. R, Diago, M, Marcellin, P, Bernstein, D, ...

Second-line chemotherapy with pemetrexed after gemcitabine failure in patients with advanced pancreatic cancer: a multicenter phase II trial (2007)

Boeck, S, Weigang-Köhler, K, Fuchs, M, Kettner, E, Quietzsch, D, Trojan, J, ...

Background: A standard second-line chemotherapy regimen has yet to be defined for patients with gemcitabine (Gem)-refractory advanced pancreatic cancer (PC). Patients and methods: In this multicenter...

Second-line chemotherapy with pemetrexed after gemcitabine failure in patients with advanced pancreatic cancer: a multicenter phase II trial (2007)

Boeck, S, Weigang-Köhler, K, Fuchs, M, Kettner, E, Quietzsch, D, Trojan, J, ...

Background: A standard second-line chemotherapy regimen has yet to be defined for patients with gemcitabine (Gem)-refractory advanced pancreatic cancer (PC). Patients and methods: In this multicenter...

IP-10 predicts viral response and therapeutic outcome in difficult-to-treat patients with HCV genotype 1 infection (2006)

Lagging, M., Romero, A.I., Westin, J., Norkrans, G., Dhillon, A.P., Pawlotsky, J.-M., ...

Plasma from 173 patients with HCV genotype 1 infection was analyzed for IP-10 levels prior to treatment with pegylated interferon--2a and ribavirin. Significantly lower IP-10 levels were observed in...

Rapid decline of viral RNA in hepatitis C patients treated with VX-950: A phase Ib, placebo-controlled, randomized study (2006)

Reesink, H.W., Zeuzem, S., Weegink, C.J., Forestier, N., Vliet, A. Van, Rooij, J. Van De Wetering, ...

Background & Aims: VX-950 specifically inhibits the NS3(.)4A protease of hepatitis C and has antiviral activity in vitro. This phase 1, placebo-controlled, double-blind study evaluated the antiviral...

Viral kinetics in patients with chronic hepatitis C treated with the serine protease inhibitor BILN 2061 (2006)

Herrmann, E., Zeuzem, S., Sarrazin, C., Hinrichsen, H., Benhamou, Y., Manns, M.P., ...

We analysed viral kinetics from a 2-day treatment with BILN 2061, a serine protease inhibitor of hepatitis C virus, in patients chronically infected with genotype 1 hepatitis C virus. The efficiency...

Relationship between early HCV kinetics and T-cell reactivity in chronic hepatitis C genotype 1 during peginterferon and ribavirin therapy (2005)

Tang, K.H., Herrmann, E., Cooksley, H., Tatman, N., Chokshi, S., Williams, R., ...

Background/aims: To gain understanding of inter-individual differences of treatment response in hepatitis C virus genotype 1 (HCV-G1) patients, we investigated simultaneously the early HCV kinetics...

Short statement of the first european consensus conference on the treatment of chronic hepatitis B and C in HIV co-infected patients (vol 42, pg 615, 2005) (2005)

Alberti, A., Clumeck, N., Collins, S., Gerlich, W., Lundgren, J.D., Palu, G., ...

Association of HCV-related mixed cryoglobulinemia with specific mutational patterns of the HCV E2 protein and CD81 expression on peripheral B lymphocytes (2004)

Hofmann,W.P., Herrmann,E., Kronenberger,B., Merkwirth,Christian, Welsch,Christian, Lengauer,Thomas, ...

Association of HCV-related mixed cryoglobulinemia with specific mutational patterns of the HCV E2 protein and CD81 expression on peripheral B lymphocytes (2004)

Hofmann, W.P., Herrmann, E., Kronenberger, B., Merkwirth, Christian, Welsch, Christoph, Lengauer, Thomas, ...

Association of HCV-related mixed cryoglobulinemia with specific mutational patterns of the HCV E2 protein and CD81 expression on peripheral B lymphocytes (2004)

Hofmann, W.P., Herrmann, E., Kronenberger, B., Merkwirth, Christian, Welsch, Christian, Lengauer, Thomas, ...

A phase I/II study of recombinant human interleukin-12 in patients with chronic hepatitis B (2000)

Carreño, V ; JFA; CORA, Zeuzem, S, Hopf, U, Marcellin, P, Cooksley, W G, Fevery, Johan; U0018702 ;, ...

BACKGROUND/AIMS: Interleukin-12 (IL-12) may be active against hepatitis B virus (HBV). The objective of the study was to assess the tolerability, activity, pharmacokinetics, and pharmacodynamics of...

Enzymatic and antisense effects of a specific anti-Ki-ras ribozyme in vitro and in cell culture (1999)

Giannini, CD, Roth, WK, Piiper, A, Zeuzem, S

Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes.

Zeuzem, S, Feick, P, Zimmermann, P, Haase, W, Kahn, R A, Schulz, I

The ADP-ribosylation factor (ARF), a highly conserved low molecular weight GTP-binding protein, has been implicated to function in intracellular protein transport to and within the Golgi complex. In...

Enzymatic and antisense effects of a specific anti-Ki-ras ribozyme in vitro and in cell culture.

Giannini, C D, Roth, W K, Piiper, A, Zeuzem, S

Due to their mode of action, ribozymes show antisense effects in addition to their specific cleavage activity. In the present study we investigated whether a hammerhead ribozyme is capable of...

Comparison of two quantitative hepatitis C virus reverse transcriptase PCR assays.

Roth, W K, Lee, J H, Rüster, B, Zeuzem, S

A quantitative hepatitis C virus reverse transcriptase PCR (HCV RT-PCR) assay established in our laboratory was compared with the Roche Amplicor HCV Monitor test kit for agreement of test results and...

Rapid microsatellite analysis of paraffin embedded tumour specimens from patients with hereditary non-polyposis colorectal cancer.

Raedle, J, Brieger, A, Trojan, J, Herrmann, G, Zeuzem, S

In screening for hereditary non-polyposis colorectal cancer (HNPCC)--an autosomal dominant disorder characterised by mutations in mismatch repair genes--detection of microsatellite instability is an...

Stimulation of inositol 1,4,5-trisphosphate production by peptides corresponding to the effector domain of different Rab3 isoforms and cross-linking of an effector domain peptide target.

Piiper, A, Stryjek-Kaminska, D, Jahn, R, Zeuzem, S

Rab3 proteins are localized on secretory vesicles and appear to be involved in regulated exocytosis. We have previously shown that a modified peptide corresponding to the effector domain of the small...

Epidermal growth factor regulates adenylate cyclase activity via Gs and Gi1-2 proteins in pancreatic acinar membranes.

Stryjek-Kaminska, D, Piiper, A, Zeuzem, S

In the present study, Western-blot and radioreceptor analyses have revealed the presence of the epidermal growth factor (EGF) receptor in pancreatic acinar membranes. Isolated pancreatic acinar...

Synthetic peptides containing a BXBXXXB(B) motif activate phospholipase C-beta1.

Piiper, A, Stryjek-Kaminska, D, Illenberger, D, Klengel, R, Schmidt, J M, Gierschik, P, ...

We have recently shown that synthetic peptides of the effector domain of the low-molecular-mass GTP-binding protein Rab3 stimulate inositol 1,4,5-trisphosphate production in various permeabilized...

Intravesicular acidification correlates with binding of ADP-ribosylation factor to microsomal membranes.

Zeuzem, S, Feick, P, Zimmermann, P, Haase, W, Kahn, R A, Schulz, I

The ADP-ribosylation factor (ARF), a highly conserved low molecular weight GTP-binding protein, has been implicated to function in intracellular protein transport to and within the Golgi complex. In...

Enzymatic and antisense effects of a specific anti-Ki-ras ribozyme in vitro and in cell culture.

Giannini, C D, Roth, W K, Piiper, A, Zeuzem, S

Due to their mode of action, ribozymes show antisense effects in addition to their specific cleavage activity. In the present study we investigated whether a hammerhead ribozyme is capable of...

Comparison of two quantitative hepatitis C virus reverse transcriptase PCR assays.

Roth, W K, Lee, J H, Rüster, B, Zeuzem, S

A quantitative hepatitis C virus reverse transcriptase PCR (HCV RT-PCR) assay established in our laboratory was compared with the Roche Amplicor HCV Monitor test kit for agreement of test results and...

Rapid microsatellite analysis of paraffin embedded tumour specimens from patients with hereditary non-polyposis colorectal cancer.

Raedle, J, Brieger, A, Trojan, J, Herrmann, G, Zeuzem, S

In screening for hereditary non-polyposis colorectal cancer (HNPCC)--an autosomal dominant disorder characterised by mutations in mismatch repair genes--detection of microsatellite instability is an...

Stimulation of inositol 1,4,5-trisphosphate production by peptides corresponding to the effector domain of different Rab3 isoforms and cross-linking of an effector domain peptide target.

Piiper, A, Stryjek-Kaminska, D, Jahn, R, Zeuzem, S

Rab3 proteins are localized on secretory vesicles and appear to be involved in regulated exocytosis. We have previously shown that a modified peptide corresponding to the effector domain of the small...

Epidermal growth factor regulates adenylate cyclase activity via Gs and Gi1-2 proteins in pancreatic acinar membranes.

Stryjek-Kaminska, D, Piiper, A, Zeuzem, S

In the present study, Western-blot and radioreceptor analyses have revealed the presence of the epidermal growth factor (EGF) receptor in pancreatic acinar membranes. Isolated pancreatic acinar...

Synthetic peptides containing a BXBXXXB(B) motif activate phospholipase C-beta1.

Piiper, A, Stryjek-Kaminska, D, Illenberger, D, Klengel, R, Schmidt, J M, Gierschik, P, ...

We have recently shown that synthetic peptides of the effector domain of the low-molecular-mass GTP-binding protein Rab3 stimulate inositol 1,4,5-trisphosphate production in various permeabilized...

Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load▿

Perrin, L., Pawlotsky, J. M., Bouvier-Alias, M., Sarrazin, C., Zeuzem, S., Colucci, G.

A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTMHIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in...

5'-CpG island methylation of the LKB1/STK11 promoter and allelic loss at chromosome 19p13.3 in sporadic colorectal cancer

Trojan, J, Brieger, A, Raedle, J, Esteller, M, Zeuzem, S

BACKGROUND—In patients with Peutz-Jeghers syndrome (PJS), causative germline mutations in the LKB1/STK11 gene on chromosome 19p13.3 have been identified. Because of the loss of heterozygosity (LOH)...

Transient mismatch repair gene transfection for functional analysis of genetic hMLH1 and hMSH2 variants

Brieger, A, Trojan, J, Raedle, J, Plotz, G, Zeuzem, S

Background: Germline mutations in the mismatch repair (MMR) genes hMLH1 and hMSH2 can cause hereditary non-polyposis colorectal cancer (HNPCC). However, the functional in vitro analysis of hMLH1 and...

An interleukin 12 p40-IgG2b fusion protein abrogates T cell mediated inflammation: anti-inflammatory activity in Crohn’s disease and experimental colitis in vivo

Stallmach, A, Marth, T, Weiß, B, Wittig, B M, Hombach, A, Schmidt, C, ...

Background and aims: Interleukin-12 (IL-12), a p35/p40 heterodimer, plays a pivotal role in the immune response in Crohn’s disease (CD). Since IL-12 p40 dimers act as IL-12 antagonists, we assayed...

Expert opinion on the treatment of patients with chronic hepatitis C

Zeuzem, S, Berg, T, Moeller, B, Hinrichsen, H, Mauss, S, Wedemeyer, H, ...

The current preferred treatment for patients with hepatitis C virus (HCV) is combination therapy consisting of pegylated interferon alfa and ribavirin (RBV) for 24–48 weeks. Although this approach...