An unusual mechanism of thymidylate biosynthesis in organisms containing the thyX gene (2009)
Eric M. Koehn, Todd Fleischmann, John A. Conrad, Bruce A. Palfey, Scott A. Lesley, Irimpan I. Mathews, ...
McCleverty, Clare J., Columbus, Linda, Kreusch, Andreas, Lesley, Scott A.
As a part of the Joint Center for Structural Genomics (JCSG) biological targets, the structures of soluble domains of membrane proteins from Thermotoga maritima were pursued. Here, we report the...
The challenge of protein structure determination lessons from structural genomics (2007)
Slabinski, Lukasz, Jaroszewski, Lukasz, Rodrigues, Ana P.C., Rychlewski, Leszek, Wilson, Ian A., Lesley, Scott A., ...
The process of experimental determination of protein structure is marred with a high ratio of failures at many stages. With availability of large quantities of data from high-throughput structure...
Huang, Sha, Romanchuk, Gail, Pattridge, Katherine, Lesley, Scott A., Wilson, Ian A., Matthews, Rowena G., ...
The crystal structure of the Thermotoga maritima gene product TM0269, determined as part of genome-wide structural coverage of T. maritima by the Joint Center for Structural Genomics, revealed...
XtalPred: a web server for prediction of protein crystallizability (2007)
Slabinski, Lukasz, Jaroszewski, Lukasz, Rychlewski, Leszek, Wilson, Ian A., Lesley, Scott A., Godzik, Adam
Summary: XtalPred is a web server for prediction of protein crystallizability. The prediction is made by comparing several features of the protein with distributions of these features in TargetDB and...
Crystal structure of the human TRPV2 channel ankyrin repeat domain (2006)
McCleverty, Clare J., Koesema, Eric, Patapoutian, Ardem, Lesley, Scott A., Kreusch, Andreas
TRPV channels are important polymodal integrators of noxious stimuli mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common protein–protein recognition domain,...
Columbus, Linda, Lipfert, Jan, Klock, Heath, Millett, Ian, Doniach, Sebastian, Lesley, Scott A.
Structural studies of integral membrane proteins typically rely upon detergent micelles as faithful mimics of the native lipid bilayer. Therefore, membrane protein structure determination would be...
Crystal structure of the human TRPV2 channel ankyrin repeat domain (2006)
McCleverty, Clare J., Koesema, Eric, Patapoutian, Ardem, Lesley, Scott A., Kreusch, Andreas
TRPV channels are important polymodal integrators of noxious stimuli mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common proteinprotein recognition domain,...
Columbus, Linda, Lipfert, Jan, Klock, Heath, Millett, Ian, Doniach, Sebastian, Lesley, Scott A.
Structural studies of integral membrane proteins typically rely upon detergent micelles as faithful mimics of the native lipid bilayer. Therefore, membrane protein structure determination would be...
Columbus, Linda, Lipfert, Jan, Klock, Heath, Millett, Ian, Doniach, Sebastian, Lesley, Scott A.
Structural studies of integral membrane proteins typically rely upon detergent micelles as faithful mimics of the native lipid bilayer. Therefore, membrane protein structure determination would be...
Crystal structure of the human TRPV2 channel ankyrin repeat domain (2006)
McCleverty, Clare J., Koesema, Eric, Patapoutian, Ardem, Lesley, Scott A., Kreusch, Andreas
TRPV channels are important polymodal integrators of noxious stimuli mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common proteinprotein recognition domain,...
Spraggon, Glen, Pantazatos, Dennis, Klock, Heath E., Wilson, Ian A., Woods, Virgil L., Lesley, Scott A.
The structure of two Thermotoga maritima proteins, a conserved hypothetical protein (TM0160) and a transcriptional regulator (TM1171), have now been determined at 1.9 Å and 2.3 Å resolution,...
Gene expression response to misfolded protein as a screen for soluble recombinant protein (2002)
Lesley, Scott A., Graziano, Jim, Cho, Charles Y., Knuth, Mark W., Klock, Heath E.
Proper protein folding is key to producing recombinant proteins for structure determination. We have examined the effect of misfolded recombinant protein on gene expression in Escherichia coli....
Landowski, Christopher P., Godfrey, Henry P., Bentley-Hibbert, Stuart I., Liu, Xinyan, Huang, Zhishan, Sepulveda, Ricardo, ...
Laboratory diagnosis of tuberculosis is often difficult. Immunodetection of circulating Mycobacterium tuberculosis proteins shed during active infection would not depend on an intact host immune...
Lesley, Scott A., Kuhn, Peter, Godzik, Adam, Deacon, Ashley M., Mathews, Irimpan, Kreusch, Andreas, ...
Structural genomics is emerging as a principal approach to define protein structure–function relationships. To apply this approach on a genomic scale, novel methods and technologies must be...
Pantazatos, Dennis, Kim, Jack S., Klock, Heath E., Stevens, Raymond C., Wilson, Ian A., Lesley, Scott A., ...
Crystallographic efforts often fail to produce suitably diffracting protein crystals. Unstructured regions of proteins play an important role in this problem and considerable advantage can be gained...
Landowski, Christopher P., Godfrey, Henry P., Bentley-Hibbert, Stuart I., Liu, Xinyan, Huang, Zhishan, Sepulveda, Ricardo, ...
Laboratory diagnosis of tuberculosis is often difficult. Immunodetection of circulating Mycobacterium tuberculosis proteins shed during active infection would not depend on an intact host immune...
Lesley, Scott A., Kuhn, Peter, Godzik, Adam, Deacon, Ashley M., Mathews, Irimpan, Kreusch, Andreas, ...
Structural genomics is emerging as a principal approach to define protein structure–function relationships. To apply this approach on a genomic scale, novel methods and technologies must be...
Pantazatos, Dennis, Kim, Jack S., Klock, Heath E., Stevens, Raymond C., Wilson, Ian A., Lesley, Scott A., ...
Crystallographic efforts often fail to produce suitably diffracting protein crystals. Unstructured regions of proteins play an important role in this problem and considerable advantage can be gained...
Columbus, Linda, Lipfert, Jan, Klock, Heath, Millett, Ian, Doniach, Sebastian, Lesley, Scott A.
Structural studies of integral membrane proteins typically rely upon detergent micelles as faithful mimics of the native lipid bilayer. Therefore, membrane protein structure determination would be...
Crystal structure of the human TRPV2 channel ankyrin repeat domain
McCleverty, Clare J., Koesema, Eric, Patapoutian, Ardem, Lesley, Scott A., Kreusch, Andreas
TRPV channels are important polymodal integrators of noxious stimuli mediating thermosensation and nociception. An ankyrin repeat domain (ARD), which is a common protein–protein recognition domain,...
Hampton, Eric N., Knuth, Mark W., Li, Jun, Harris, Jennifer L., Lesley, Scott A., Spraggon, Glen
Mutations in proprotein convertase subtilisin/kexin type 9 (PCSK9) are strongly associated with levels of low-density lipoprotein cholesterol in the blood plasma and, thereby, occurrence or...
Spraggon, Glen, Pantazatos, Dennis, Klock, Heath E., Wilson, Ian A., Woods, Virgil L., Lesley, Scott A.
The structure of two Thermotoga maritima proteins, a conserved hypothetical protein (TM0160) and a transcriptional regulator (TM1171), have now been determined at 1.9 Å and 2.3 Å resolution,...
Huang, Sha, Romanchuk, Gail, Pattridge, Katherine, Lesley, Scott A., Wilson, Ian A., Matthews, Rowena G., ...
The crystal structure of the Thermotoga maritima gene product TM0269, determined as part of genome-wide structural coverage of T. maritima by the Joint Center for Structural Genomics, revealed...
The challenge of protein structure determination—lessons from structural genomics
Slabinski, Lukasz, Jaroszewski, Lukasz, Rodrigues, Ana P.C., Rychlewski, Leszek, Wilson, Ian A., Lesley, Scott A., ...
The process of experimental determination of protein structure is marred with a high ratio of failures at many stages. With availability of large quantities of data from high-throughput structure...
McCleverty, Clare J., Columbus, Linda, Kreusch, Andreas, Lesley, Scott A.
As a part of the Joint Center for Structural Genomics (JCSG) biological targets, the structures of soluble domains of membrane proteins from Thermotoga maritima were pursued. Here, we report the...
Metabolomics analysis reveals large effects of gut microflora on mammalian blood metabolites
Wikoff, William R., Anfora, Andrew T., Liu, Jun, Schultz, Peter G., Lesley, Scott A., Peters, Eric C., ...
Although it has long been recognized that the enteric community of bacteria that inhabit the human distal intestinal track broadly impacts human health, the biochemical details that underlie these...
The Thermotoga maritima Trk Potassium Transporter—from Frameshift to Function ▿
Johnson, Hope A., Hampton, Eric, Lesley, Scott A.
The gene for the Thermotoga maritima Trk potassium transporter component TrkA was originally thought to be a frameshift mutation and not to encode a functional protein. However, expression from this...
Bacterial Pleckstrin Homology Domains: A Prokaryotic Origin for the PH Domain
Xu, Qingping, Bateman, Alex, Finn, Robert D., Abdubek, Polat, Astakhova, Tamara, Axelrod, Herbert L., ...
Pleckstrin homology (PH) domains have been identified only in eukaryotic proteins to date. We have determined crystal structures for three members of an uncharacterized protein family (Pfam PF08000),...