Aminoglycoside activity observed on single pre-translocation ribosome complexes (2010)
Michael B Feldman, Daniel S Terry, Roger B Altman, Scott C Blanchard
Aminoglycoside activity observed on single pre-translocation ribosome complexes (2009)
Michael B Feldman, Daniel S Terry, Roger B Altman, Scott C Blanchard
Site-specific labeling of the ribosome for single-molecule spectroscopy (2005)
Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach...
tRNA selection and kinetic proofreading in translation (2004)
Scott C Blanchard, Ruben L Gonzalez, Harold D Kim, Steven Chu, Joseph D Puglisi
Solution structure of the A loop of 23S ribosomal RNA
Blanchard, Scott C., Puglisi, Joseph D.
The A loop is an essential RNA component of the ribosome peptidyltransferase center that directly interacts with aminoacyl (A)-site tRNA. The A loop is highly conserved and contains a ubiquitous...
tRNA dynamics on the ribosome during translation
Blanchard, Scott C., Kim, Harold D., Gonzalez, Ruben L., Puglisi, Joseph D., Chu, Steven
Using single-molecule fluorescence spectroscopy, time-resolved conformational changes between fluorescently labeled tRNA have been characterized within surface-immobilized ribosomes proceeding...
Site-specific labeling of the ribosome for single-molecule spectroscopy
Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach...
Solution structure of the A loop of 23S ribosomal RNA
Blanchard, Scott C., Puglisi, Joseph D.
The A loop is an essential RNA component of the ribosome peptidyltransferase center that directly interacts with aminoacyl (A)-site tRNA. The A loop is highly conserved and contains a ubiquitous...
tRNA dynamics on the ribosome during translation
Blanchard, Scott C., Kim, Harold D., Gonzalez, Ruben L., Puglisi, Joseph D., Chu, Steven
Using single-molecule fluorescence spectroscopy, time-resolved conformational changes between fluorescently labeled tRNA have been characterized within surface-immobilized ribosomes proceeding...
Site-specific labeling of the ribosome for single-molecule spectroscopy
Dorywalska, Magdalena, Blanchard, Scott C., Gonzalez, Ruben L., Kim, Harold D., Chu, Steven, Puglisi, Joseph D.
Single-molecule fluorescence spectroscopy can reveal mechanistic and kinetic details that may not be observed in static structural and bulk biochemical studies of protein synthesis. One approach...
The role of fluctuations in tRNA selection by the ribosome
Lee, Tae-Hee, Blanchard, Scott C., Kim, Harold D., Puglisi, Joseph D., Chu, Steven
The detailed mechanism of how the ribosome decodes protein sequence information with an abnormally high accuracy, after 40 years of study, remains elusive. A critical element in selecting correct...