Olga Rechkoblit, Alexander Kolbanovskiy, Lucy Malinina, Nicholas E Geacintov, Suse Broyde, Dinshaw J Patel
Donny-Clark, Kerry, Broyde, Suse
Human DNA polymerase &igr; is a lesion bypass polymerase of the Y family, capable of incorporating nucleotides opposite a variety of lesions in both near error-free and error-prone bypass. With...
Dimitri, Alexandra, Burns, John A., Broyde, Suse, Scicchitano, David A.
O6-Methylguanine (O6-meG) is a major mutagenic, carcinogenic and cytotoxic DNA adduct produced by various endogenous and exogenous methylating agents. We report the results of transcription past a...
Jia, Lei, Geacintov, Nicholas E., Broyde, Suse
DNA bypass polymerases are utilized to transit bulky DNA lesions during replication, but the process frequently causes mutations. The structural origins of mutagenic versus high fidelity replication...
Xu, Pingna, Oum, Lida, Beese, Lorena S., Geacintov, Nicholas E., Broyde, Suse
We have investigated how a benzo[a]pyrene-derived N2-dG adduct, 10S(+)-trans-anti-[BP]-N2-dG ([BP]G*), is processed in a well-characterized Pol I family model replicative DNA polymerase, Bacillus...
Rodríguez, Fabián A., Cai, Yuqin, Lin, Chin, Tang, Yijin, Kolbanovskiy, Alexander, Amin, Shantu, ...
The environmental carcinogen benzo[a]pyrene (BP) is metabolized to reactive diol epoxides that bind to cellular DNA by predominantly forming N2-guanine adducts (G*). Mutation hotspots for these...
Rodríguez, Fabián A., Cai, Yuqin, Lin, Chin, Tang, Yijin, Kolbanovskiy, Alexander, Amin, Shantu, ...
The environmental carcinogen benzo[a]pyrene (BP) is metabolized to reactive diol epoxides that bind to cellular DNA by predominantly forming N2-guanine adducts (G*). Mutation hotspots for these...
Rodríguez, Fabián A., Cai, Yuqin, Lin, Chin, Tang, Yijin, Kolbanovskiy, Alexander, Amin, Shantu, ...
The environmental carcinogen benzo[a]pyrene (BP) is metabolized to reactive diol epoxides that bind to cellular DNA by predominantly forming N2-guanine adducts (G*). Mutation hotspots for these...
Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion (2006)
Olga Rechkoblit, Lucy Malinina, Yuan Cheng, Vitaly Kuryavyi, Suse Broyde, Nicholas E. Geacintov, ...
Crystal structures of the bypass polymerase Dpo4 at different stages of lesion-bypass reveal how the cell copes with oxidative DNA damage.
Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion (2006)
Olga Rechkoblit, Lucy Malinina, Yuan Cheng, Vitaly Kuryavyi, Suse Broyde, Nicholas E. Geacintov, ...
7,8-dihydro-8-oxoguanine (oxoG), the predominant lesion formed following oxidative damage of DNA by reactive oxygen species, is processed differently by replicative and bypass polymerases. Our...
Zhang, Ling, Rechkoblit, Olga, Wang, Lihua, Patel, Dinshaw J., Shapiro, Robert, Broyde, Suse
Bulky carcinogen-DNA adducts commonly cause replicative polymerases to stall, leading to a switch to bypass polymerases. We have investigated nucleotide incorporation opposite the major adduct of...
Primer extension studies have shown that the Y-family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus P2 can preferentially insert C opposite N-(deoxyguanosin-8-yl)-2-acetylaminofluorene...
10.1098/rsta.2004.1386 Deformations of promoter DNA bound to (2004)
Qing Zhang, Suse Broyde, Tamar Schlick
carcinogens help interpret effects on TATA-element structure and activity
Schinecker, Thomas M., Perlow, Rebecca A., Broyde, Suse, Geacintov, Nicholas E., Scicchitano, David A.
Environmental polycyclic aromatic hydrocarbons (PAHs) are metabolically activated to diol epoxides that can react with DNA, resulting in covalent modifications to the bases. The (+)‐ and...
Wu, Min, Yan, Shixiang, Patel, Dinshaw J., Geacintov, Nicholas E., Broyde, Suse
A key issue in the nucleotide excision repair (NER) of bulky carcinogen–DNA adducts is the ability of the NER machinery to recognize and repair certain adducts while failing to repair others....
Nolan, Scott J., Vyas, Rajeev R., Hingerty, Brian E., Ellis, Stephen, Broyde, Suse, Shapiro, Robert, ...
An oligodeoxyribonucleotide 5' -d(CTCATGAPATTCC), in which GAP denotes N-(guanin-8-yl)-1-aminopyrene, the C8-guanine adduct of reductively activated 1-nitropyrene, was synthesized and characterized...
O'Handley, Suzanne, Sanford, David, Xu, Rong, Lester, Cathy, Hingerty, Brian, Broyde, Suse, ...
Article may be found at: http://pubs3.acs.org/acs/journals/toc.page?incoden=bichaw&indecade=1&involume=32&inissue=10
O'Handley, Suzanne, Sanford, David, Xu, Rong, Lester, Cathy, Hingerty, Brian, Broyde, Suse, ...
Article may be found at: http://pubs3.acs.org/acs/journals/toc.page?incoden=bichaw&indecade=1&involume=32&inissue=10
O'Handley, Suzanne, Sanford, David, Xu, Rong, Lester, Cathy, Hingerty, Brian, Broyde, Suse, ...
Article may be found at: http://pubs3.acs.org/acs/journals/toc.page?incoden=bichaw&indecade=1&involume=32&inissue=10
Broyde, Suse, Hingerty, Brian E.
Energy minimized structures of base displacement in an AAF modified B-DNA dodecamer are presented. A rational search strategy, beginning with a global search of the conformation space of the modified...
Neidle, Stephen, Kuroda, Reiko, Broyde, Suse, Hingerty, Brian E., Levine, Robert A., Miller, Dwight W., ...
The structure of an adduct between guanine and the carcinogen acetylaminofluorene has been examined in the solid state by X-ray crystallography, and in solution by NMR techniques. The observed...
Santella, Regina M., Grunberger, Dezider, Broyde, Suse, Hingerty, Brian E.
The conformation of poly(dG-dC)·poly(dG-dC), poly(dG)·poly(dC), and calf thymus DNA modified with N-acetoxy-N-2-acetylaminofluorene (N-acetoxy-AAF) was examined by extent of reaction with anti...
Brown, Karen, Hingerty, Brian E., Guenther, Elizabeth A., Krishnan, V. V., Broyde, Suse, Turteltaub, Kenneth W., ...
The carcinogenic heterocyclic amine (HA) 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is formed during the cooking of various meats. To enable structure/activity studies aimed at...
Wu, Min, Yan, Shixiang, Patel, Dinshaw J., Geacintov, Nicholas E., Broyde, Suse
A key issue in the nucleotide excision repair (NER) of bulky carcinogen–DNA adducts is the ability of the NER machinery to recognize and repair certain adducts while failing to repair others....
Schinecker, Thomas M., Perlow, Rebecca A., Broyde, Suse, Geacintov, Nicholas E., Scicchitano, David A.
Environmental polycyclic aromatic hydrocarbons (PAHs) are metabolically activated to diol epoxides that can react with DNA, resulting in covalent modifications to the bases. The (+)- and...
Accurate representation of B-DNA double helical structure with implicit solvent and counterions.
Wang, Lihua, Hingerty, Brian E, Srinivasan, A R, Olson, Wilma K, Broyde, Suse
High-resolution nuclear magnetic resonance (NMR) and crystallographic data have been taken to refine the force field used in the torsion angle space nucleic acids molecular mechanics program DUPLEX....
Simulating Structural and Thermodynamic Properties of Carcinogen-Damaged DNA
Yan, Shixiang, Wu, Min, Patel, Dinshaw J., Geacintov, Nicholas E., Broyde, Suse
A pair of stereoisomeric covalent adducts to guanine in double-stranded DNA, derived from the reaction of mutagenic and tumorigenic metabolites of benzo[a]pyrene, have been well characterized...
Yang, Linjing, Beard, William A., Wilson, Samuel H., Broyde, Suse, Schlick, Tamar
To link conformational transitions noted for DNA polymerases with kinetic results describing catalytic efficiency and fidelity, we investigate the role of key DNA polymerase β residues on subdomain...
Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion
Rechkoblit, Olga, Malinina, Lucy, Cheng, Yuan, Kuryavyi, Vitaly, Broyde, Suse, Geacintov, Nicholas E, ...
7,8-dihydro-8-oxoguanine (oxoG), the predominant lesion formed following oxidative damage of DNA by reactive oxygen species, is processed differently by replicative and bypass polymerases. Our...
Primer extension studies have shown that the Y-family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus P2 can preferentially insert C opposite N-(deoxyguanosin-8-yl)-2-acetylaminofluorene...
Zhang, Ling, Rechkoblit, Olga, Wang, Lihua, Patel, Dinshaw J., Shapiro, Robert, Broyde, Suse
Bulky carcinogen-DNA adducts commonly cause replicative polymerases to stall, leading to a switch to bypass polymerases. We have investigated nucleotide incorporation opposite the major adduct of...
Brown, Karen, Hingerty, Brian E., Guenther, Elizabeth A., Krishnan, V. V., Broyde, Suse, Turteltaub, Kenneth W., ...
The carcinogenic heterocyclic amine (HA) 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is formed during the cooking of various meats. To enable structure/activity studies aimed at...
Wu, Min, Yan, Shixiang, Patel, Dinshaw J., Geacintov, Nicholas E., Broyde, Suse
A key issue in the nucleotide excision repair (NER) of bulky carcinogen–DNA adducts is the ability of the NER machinery to recognize and repair certain adducts while failing to repair others....
Schinecker, Thomas M., Perlow, Rebecca A., Broyde, Suse, Geacintov, Nicholas E., Scicchitano, David A.
Environmental polycyclic aromatic hydrocarbons (PAHs) are metabolically activated to diol epoxides that can react with DNA, resulting in covalent modifications to the bases. The (+)- and...
Accurate representation of B-DNA double helical structure with implicit solvent and counterions.
Wang, Lihua, Hingerty, Brian E, Srinivasan, A R, Olson, Wilma K, Broyde, Suse
High-resolution nuclear magnetic resonance (NMR) and crystallographic data have been taken to refine the force field used in the torsion angle space nucleic acids molecular mechanics program DUPLEX....
Simulating Structural and Thermodynamic Properties of Carcinogen-Damaged DNA
Yan, Shixiang, Wu, Min, Patel, Dinshaw J., Geacintov, Nicholas E., Broyde, Suse
A pair of stereoisomeric covalent adducts to guanine in double-stranded DNA, derived from the reaction of mutagenic and tumorigenic metabolites of benzo[a]pyrene, have been well characterized...
Yang, Linjing, Beard, William A., Wilson, Samuel H., Broyde, Suse, Schlick, Tamar
To link conformational transitions noted for DNA polymerases with kinetic results describing catalytic efficiency and fidelity, we investigate the role of key DNA polymerase β residues on subdomain...
Stepwise Translocation of Dpo4 Polymerase during Error-Free Bypass of an oxoG Lesion
Rechkoblit, Olga, Malinina, Lucy, Cheng, Yuan, Kuryavyi, Vitaly, Broyde, Suse, Geacintov, Nicholas E, ...
7,8-dihydro-8-oxoguanine (oxoG), the predominant lesion formed following oxidative damage of DNA by reactive oxygen species, is processed differently by replicative and bypass polymerases. Our...
Primer extension studies have shown that the Y-family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus P2 can preferentially insert C opposite N-(deoxyguanosin-8-yl)-2-acetylaminofluorene...
Zhang, Ling, Rechkoblit, Olga, Wang, Lihua, Patel, Dinshaw J., Shapiro, Robert, Broyde, Suse
Bulky carcinogen-DNA adducts commonly cause replicative polymerases to stall, leading to a switch to bypass polymerases. We have investigated nucleotide incorporation opposite the major adduct of...
Rodríguez, Fabián A., Cai, Yuqin, Lin, Chin, Tang, Yijin, Kolbanovskiy, Alexander, Amin, Shantu, ...
The environmental carcinogen benzo[a]pyrene (BP) is metabolized to reactive diol epoxides that bind to cellular DNA by predominantly forming N2-guanine adducts (G*). Mutation hotspots for these...
Xu, Pingna, Oum, Lida, Beese, Lorena S., Geacintov, Nicholas E., Broyde, Suse
We have investigated how a benzo[a]pyrene-derived N2-dG adduct, 10S(+)-trans-anti-[BP]-N2-dG ([BP]G*), is processed in a well-characterized Pol I family model replicative DNA polymerase, Bacillus...
The human DNA repair factor XPC-HR23B distinguishes stereoisomeric benzo[a]pyrenyl-DNA lesions
Mocquet, Vincent, Kropachev, Konstantin, Kolbanovskiy, Marina, Kolbanovskiy, Alexander, Tapias, Angels, Cai, Yuqin, ...
Benzo[a]pyrene (B[a]P), a known environmental pollutant and tobacco smoke carcinogen, is metabolically activated to highly tumorigenic B[a]P diol epoxide derivatives that predominantly form...
Dimitri, Alexandra, Burns, John A., Broyde, Suse, Scicchitano, David A.
O6-Methylguanine (O6-meG) is a major mutagenic, carcinogenic and cytotoxic DNA adduct produced by various endogenous and exogenous methylating agents. We report the results of transcription past a...
Jia, Lei, Geacintov, Nicholas E., Broyde, Suse
DNA bypass polymerases are utilized to transit bulky DNA lesions during replication, but the process frequently causes mutations. The structural origins of mutagenic versus high fidelity replication...
Donny-Clark, Kerry, Broyde, Suse
Human DNA polymerase ι is a lesion bypass polymerase of the Y family, capable of incorporating nucleotides opposite a variety of lesions in both near error-free and error-prone bypass. With...