Scientific Commons: T. A. Holak

Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact,...

Filamin-regulated F-actin assembly is essential for morphogenesis and controls phototaxis in Dictyostelium (2007)

Khaire, N., Muller, R., Blau-Wasser, R., Eichinger, L., Schleicher, M., Rief, M., ...

Protein solubility: sequence based prediction and experimental verification (2007)

Smialowski, P., Martin-Galiano, A. J., Mikolajka, A., Girschick, T., Holak, T. A., Frishman, D.

CAP2, cyclase-associated protein 2, is a dual compartment protein (2007)

Peche, V., Shekar, S., Leichter, M., Korte, H., Schroder, R., Schleicher, M., ...

An NMR-Based antagonist induced dissociation assay for targeting the ligand-protein and protein-protein interactions in competition binding experiments (2007)

Krajewski, M., Rothweiler, U., D'Silva, L., Majumdar, S., Klein, C., Holak, T. A.

Structural ramification for acetyl-lysine recognition by the bromodomain of human BRG1 protein, a central ATPase of the SWI/SNF remodeling complex (2007)

Singh, M., Popowicz, G. M., Krajewski, M., Holak, T. A.

Molecular basis for the inhibition of p53 by Mdmx (2007)

Popowicz, G. M., Czarna, A., Rothweiler, U., Szwagierczak, A., Krajewski, M., Weber, L., ...

DNA-binding properties of the recombinant high-mobility-group-like AT- hook-containing region from human BRG1 protein (2006)

Singh, M., D'Silva, L., Holak, T. A.

Filamins: promiscuous organizers of the cytoskeleton (2006)

Popowicz, G. M., Schleicher, M., Noegel, A. A., Holak, T. A.

Structure of the N-terminal domain of the FOP (FGFR1OP) protein and implications for its dimerization and centrosomal localization (2006)

Mikolajka, A., Yan, X. M., Popowicz, G. M., Smialowski, P., Nigg, E. A., Holak, T. A.

Functional and structural characterization of Spl proteases from Staphylococcus aureus (2006)

Popowicz, G. M., Dubin, G., Stec-Niemczyk, J., Czarny, A., Dubin, A., Potempa, J., ...

Identification of calpain cleavage sites in the G1 cyclin-dependent kinase inhibitor p19(INK4d) (2006)

Joy, J., Nalabothula, N., Ghosh, M., Popp, O., Jochum, M., Machleidt, W., ...

DNA-binding properties of the recombinant high-mobility-group-like AT- hook-containing region from human BRG1 protein (2006)

Singh, M., D'Silva, L., Holak, T. A.

Characterization of 14-3-3sigma dimerization determinants (2006)

Verdoodt, B., Benzinger, A., Popowicz, G. M., Holak, T. A., Hermeking, H.

Molecular determinants for the complex formation between the retinoblastoma protein and LXCXE sequences (2005)

Singh,M., Krajewski,M., Mikolajka,A., Holak,T. A.

NMR indicates that the small molecule RITA does not block p53-MDM2 binding in vitro (2005)

Krajewski,M., Ozdowy,P., D'Silva,L., Rothweiler,U., Holak,T. A.

Study of a major intermediate in the oxidative folding of leech carboxypeptidase inhibitor: Contribution of the fourth disulfide bond (2005)

Arolas,J. L., Popowicz,G. M., Bronsoms,S., Aviles,F. X., Huber,R., Holak,T. A., ...

Monitoring the effects of antagonists on protein-protein interactions with NMR spectroscopy (2005)

D'Silva,L., Ozdowy,P., Krajewski,M., Rothweiler,U., Singh,M., Holak,T. A.

NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor (2005)

Arolas,J. L., D'Silva,L., Popowicz,G. M., Aviles,F. X., Holak,T. A., Ventura,S.

The three-dimensional structures of tick carboxypeptidase inhibitor in complex with A/B carboxypeptidases reveal a novel double-headed binding mode (2005)

Arolas,J. L., Popowicz,G. M., Lorenzo,J., Sornmerhoff,C. P., Huber,R., Aviles,F. X., ...

Structural and functional characterization of a novel carboxypeptidase inhibitor from ticks (2005)

Arolas,J. L., Popowicz,G., Lorenzo,J., Holak,T. A., Sommerhoff,C. P., Aviles,F. X.

NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb and CDK6 with cell differentiation proteins MyoD and ID-2 (2005)

Smialowski,P., Singh,M., Mikolajka,A., Majumdar,S., Joy,J. K., Nalabothula,N., ...

The crystal structure of the non-liganded 14-3-3 sigma protein: insights into determinants of isoform specific ligand binding and dimerization (2005)

Benzinger,A., Popowicz,G. M., Joy,J. K., Majumdar,S., Holak,T. A., Hermeking,H.

Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain (2005)

Ghosh,M., Shanker,S., Siwanowicz,I., Mann,K., Machleidt,W., Holak,T. A.

Structural basis for the regulation of insulin-like growth factors by IGF binding proteins (2005)

Siwanowicz,I., Popowicz,G. M., Wisniewska,M., Huber,R., Kuenkele,K. P., Lang,K., ...

Structural basis for the regulation of insulin-like growth factors by IGF binding proteins (2005)

Siwanowicz, I., Popowicz, G. M., Wisniewska, M., Huber, R., Kuenkele, K. P., Lang, K., ...

Molecular determinants for the complex formation between the retinoblastoma protein and LXCXE sequences (2005)

Singh, M., Krajewski, M., Mikolajka, A., Holak, T. A.

Monitoring the effects of antagonists on protein-protein interactions with NMR spectroscopy (2005)

D'Silva, L., Ozdowy, P., Krajewski, M., Rothweiler, U., Singh, M., Holak, T. A.

NMR and mass spectrometry studies of putative interactions of cell cycle proteins pRb and CDK6 with cell differentiation proteins MyoD and ID-2 (2005)

Smialowski, P., Singh, M., Mikolajka, A., Majumdar, S., Joy, J. K., Nalabothula, N., ...

NMR indicates that the small molecule RITA does not block p53-MDM2 binding in vitro (2005)

Krajewski, M., Ozdowy, P., D'Silva, L., Rothweiler, U., Holak, T. A.

NMR structural characterization and computational predictions of the major intermediate in oxidative folding of leech carboxypeptidase inhibitor (2005)

Arolas, J. L., D'Silva, L., Popowicz, G. M., Aviles, F. X., Holak, T. A., Ventura, S.

Structural and functional characterization of a novel carboxypeptidase inhibitor from ticks (2005)

Arolas, J. L., Popowicz, G., Lorenzo, J., Holak, T. A., Sommerhoff, C. P., Aviles, F. X.

Study of a major intermediate in the oxidative folding of leech carboxypeptidase inhibitor: Contribution of the fourth disulfide bond (2005)

Arolas, J. L., Popowicz, G. M., Bronsoms, S., Aviles, F. X., Huber, R., Holak, T. A., ...

The crystal structure of the non-liganded 14-3-3 sigma protein: insights into determinants of isoform specific ligand binding and dimerization (2005)

Benzinger, A., Popowicz, G. M., Joy, J. K., Majumdar, S., Holak, T. A., Hermeking, H.

The three-dimensional structures of tick carboxypeptidase inhibitor in complex with A/B carboxypeptidases reveal a novel double-headed binding mode (2005)

Arolas, J. L., Popowicz, G. M., Lorenzo, J., Sornmerhoff, C. P., Huber, R., Aviles, F. X., ...

Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain (2005)

Ghosh, M., Shanker, S., Siwanowicz, I., Mann, K., Machleidt, W., Holak, T. A.

Molecular structure of the rod domain of Dictyostelium filamin (2004)

Popowicz,G. M., Muller,R., Noegel,A. A., Schleicher,M., Huber,R., Holak,T. A.

NMR structural characterization of the N-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum (2004)

Mavoungou,C., Israel,L., Rehm,T., Ksiazek,D., Krajewski,M., Popowicz,G., ...

Letter to the Editor: H-1, N-15 and C-13 NMR resonance assignments of staphostatin A, a specific Staphylococcus aureus cysteine proteinase inhibitor (2004)

Dubin,G., Popowicz,G., Krajewski,M., Potempa,J., Dubin,A., Holak,T. A.

Efforts towards the design of 'Teflon' proteins: In vivo translation with trifluorinated leucine and methionine analogues (2004)

Budisa,N., Pipitone,O., Siwanowicz,I., Rubini,M., Pal,P. P., Holak,T. A., ...

Letter to the Editor: H-1, N-15 and C-13 NMR resonance assignments of staphostatin A, a specific Staphylococcus aureus cysteine proteinase inhibitor (2004)

Dubin, G., Popowicz, G., Krajewski, M., Potempa, J., Dubin, A., Holak, T. A.

Efforts towards the design of 'Teflon' proteins: In vivo translation with trifluorinated leucine and methionine analogues (2004)

Budisa, N., Pipitone, O., Siwanowicz, I., Rubini, M., Pal, P. P., Holak, T. A., ...

Molecular structure of the rod domain of Dictyostelium filamin (2004)

Popowicz, G. M., Muller, R., Noegel, A. A., Schleicher, M., Huber, R., Holak, T. A.

NMR structural characterization of the N-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum (2004)

Mavoungou, C., Israel, L., Rehm, T., Ksiazek, D., Krajewski, M., Popowicz, G., ...

A novel class of cysteine protease inhibitors: Solution structure of staphostatin A from Staphylococcus aureus (2003)

Dubin,G., Krajewski,M., Popowicz,G., Stec-Niemczyk,J., Bochtler,M., Potempa,J., ...

Active detachment involves inhibition of cell-matrix contacts of malignant melanoma cells by secretion of melanoma inhibitory activity (2003)

Bosserhoff,A. K., Stoll,R., Sleeman,J. P., Bataille,F., Buettner,R., Holak,T. A.

Structure of the n-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum (2003)

Ksiazek,D., Brandstetter,H., Israel,L., Bourenkov,G. P., Katchalova,G., Janssen,K. P., ...

Expansion of the genetic code enables design of a novel "gold'' class of green fluorescent proteins (2003)

Bae,J. H., Rubini,M., Jung,G., Wiegand,G., Seifert,M. H. J., Azim,M. K., ...

Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical...

A novel medium for expression of proteins selectively labeled with N-15-amino acids in Spodoptera frugiperda (Sf9) insect cells (2003)

Bruggert,M., Rehm,T., Shanker,S., Georgescu,J., Holak,T. A.

Whereas bacterial expression systems are widely used for production of uniformly or selectively N-15-labeled proteins the usage of the baculovirus expression system for labeling is limited to very...

Backbone dynamics of green fluorescent protein and the effect of histidine 148 substitution (2003)

Seifert,M. H. J., Georgescu,J., Ksiazek,D., Smialowski,P., Rehm,T., Steipe,B., ...

Green fluorescent protein (GFP) and its mutants have become valuable tools in molecular biology. GFP has been regarded as a very stable and rigid protein with the beta-barrel shielding the...

Backbone dynamics of the human MIA protein studied by N-15 NMR relaxation: Implications for extended interactions of SH3 domains (2003)

Stoll,R., Renner,C., Buettner,R., Voelter,W., Bosserhoff,A. K., Holak,T. A.

The melanoma inhibitory activity (MIA) protein is a clinically valuable marker in patients with malignant melanoma as enhanced values diagnose metastatic melanoma stages III and IV. Here, we report...

Backbone dynamics of green fluorescent protein and the effect of histidine 148 substitution (2003)

Seifert, M. H. J., Georgescu, J., Ksiazek, D., Smialowski, P., Rehm, T., Steipe, B., ...

Green fluorescent protein (GFP) and its mutants have become valuable tools in molecular biology. GFP has been regarded as a very stable and rigid protein with the beta-barrel shielding the...

Backbone dynamics of the human MIA protein studied by N-15 NMR relaxation: Implications for extended interactions of SH3 domains (2003)

Stoll, R., Renner, C., Buettner, R., Voelter, W., Bosserhoff, A. K., Holak, T. A.

The melanoma inhibitory activity (MIA) protein is a clinically valuable marker in patients with malignant melanoma as enhanced values diagnose metastatic melanoma stages III and IV. Here, we report...

Expansion of the genetic code enables design of a novel "gold'' class of green fluorescent proteins (2003)

Bae, J. H., Rubini, M., Jung, G., Wiegand, G., Seifert, M. H. J., Azim, M. K., ...

Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical...

A novel medium for expression of proteins selectively labeled with N-15-amino acids in Spodoptera frugiperda (Sf9) insect cells (2003)

Bruggert, M., Rehm, T., Shanker, S., Georgescu, J., Holak, T. A.

Whereas bacterial expression systems are widely used for production of uniformly or selectively N-15-labeled proteins the usage of the baculovirus expression system for labeling is limited to very...

A novel class of cysteine protease inhibitors: Solution structure of staphostatin A from Staphylococcus aureus (2003)

Dubin, G., Krajewski, M., Popowicz, G., Stec-Niemczyk, J., Bochtler, M., Potempa, J., ...

Active detachment involves inhibition of cell-matrix contacts of malignant melanoma cells by secretion of melanoma inhibitory activity (2003)

Bosserhoff, A. K., Stoll, R., Sleeman, J. P., Bataille, F., Buettner, R., Holak, T. A.

Structure of the n-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum (2003)

Ksiazek, D., Brandstetter, H., Israel, L., Bourenkov, G. P., Katchalova, G., Janssen, K. P., ...

Structure of the N-terminal domain of the adenylyl cyclase-associated protein (CAP) from Dictyostelium discoideum (2003)

Ksiazek, D., Brandstetter, H., Israel, L., Bourenkov, G. P., Kachalova, G., Janssen, K. P., ...

Active detachment involves inhibition of cell-matrix contacts of malignant melanoma cells by secretion of melanoma inhibitory activity. (2003)

Bosserhoff, A.K., Stoll, R., Sleeman, J.P., Bataille, F., Buettner, R., Holak, T.A.

Laboratory Investigation, 83(2003) S.1583-94

In silico and NMR identification of inhibitors of the IGF-I and IGF-binding protein-5 interaction (2002)

Kamionka,M., Rehm,T., Beisel,H. G., Lang,K., Engh,R. A., Holak,T. A.

Recently we have determined the crystal structure of the insulin-like growth factor-I (IGF-I) in complex with the N- terminal domain of the IGF-binding protein-5 (IGFBP-5). Here we report results of...

Application of NMR in structural proteomics: Screening for proteins amenable to structural analysis (2002)

Rehm,T., Huber,R., Holak,T. A.

In the time of structural proteomics when protein structures are targeted on a genome-wide scale, the detection of "well- behaved" proteins that would yield good quality NMR spectra or X-ray images...

Purification, crystallization, NMR spectroscopy and biochemical analyses of alpha-phycoerythrocyanin peptides (2002)

Wiegand,G., Parbel,A., Seifert,M. H. J., Holak,T. A., Reuter,W.

The alpha-phycoerythrocyanin subunits of the different phycoerythrocyanin complexes of the phycobilisomes from the cyanobacterium Mastigocladus laminosus perform a remarkable photochemistry. Similar...

Letter to the editor: Sequence-specific (H-1, N-15, C-13) resonance assignment of the N-terminal domain of the cyclase- associated protein (CAP) from Dictyostelium discoideum (2002)

Rehm,T., Mavoungou,C., Israel,L., Schleicher,M., Holak,T. A.

Slow exchange in the chromophore of a green fluorescent protein variant (2002)

Seifert,M. H., Ksiazek,D., Azim,M. K., Smialowski,P., Budisa,N., Holak,T. A.

Green fluorescent protein and its mutants have become valuable tools in molecular biology. They also provide systems rich in photophysical and photochemical phenomena of which an understanding is...

Phosphorylation and flexibility of cyclic-AMP-dependent protein kinase (PKA) using P-31 NMR Spectroscopy (2002)

Seifert,M. H. J., Breitenlechner,C. B., Bossemeyer,D., Huber,R., Holak,T. A., Engh,R. A.

Cell signaling pathways rely on phosphotransfer reactions that are catalyzed by protein kinases. The protein kinases themselves are typically regulated by phosphorylation and concurrent structural...

Practical aspects of the 2D N-15-{H-1}-NOE experiment (2002)

Renner,C., Schleicher,M., Moroder,L., Holak,T. A.

The heteronuclear N-15-NOE experiment was extensively tested with respect to statistical and systematic experimental error. The dependence of signal intensity in the NOE experiment and in the...

Protein folding and stability of human CDK inhibitor p19(INK4d) (2002)

Zeeb,M., Rosner,H., Zeslawski,W., Canet,D., Holak,T. A., Balbach,J.

P19(INK4d) is a tumor suppressing protein and belongs to a family of cyclin D-dependent kinase inhibitors of CDK4 and CDK6, which play a key role in human cell cycle control. P19 comprises ten...

Slow exchange in the chromophore of a green fluorescent protein variant (2002)

Seifert, M. H., Ksiazek, D., Azim, M. K., Smialowski, P., Budisa, N., Holak, T. A.

Green fluorescent protein and its mutants have become valuable tools in molecular biology. They also provide systems rich in photophysical and photochemical phenomena of which an understanding is...

Practical aspects of the 2D N-15-{H-1}-NOE experiment (2002)

Renner, C., Schleicher, M., Moroder, L., Holak, T. A.

The heteronuclear N-15-NOE experiment was extensively tested with respect to statistical and systematic experimental error. The dependence of signal intensity in the NOE experiment and in the...

Protein folding and stability of human CDK inhibitor p19(INK4d) (2002)

Zeeb, M., Rosner, H., Zeslawski, W., Canet, D., Holak, T. A., Balbach, J.

P19(INK4d) is a tumor suppressing protein and belongs to a family of cyclin D-dependent kinase inhibitors of CDK4 and CDK6, which play a key role in human cell cycle control. P19 comprises ten...

Purification, crystallization, NMR spectroscopy and biochemical analyses of alpha-phycoerythrocyanin peptides (2002)

Wiegand, G., Parbel, A., Seifert, M. H. J., Holak, T. A., Reuter, W.

The alpha-phycoerythrocyanin subunits of the different phycoerythrocyanin complexes of the phycobilisomes from the cyanobacterium Mastigocladus laminosus perform a remarkable photochemistry. Similar...

Phosphorylation and flexibility of cyclic-AMP-dependent protein kinase (PKA) using P-31 NMR Spectroscopy (2002)

Seifert, M. H. J., Breitenlechner, C. B., Bossemeyer, D., Huber, R., Holak, T. A., Engh, R. A.

Cell signaling pathways rely on phosphotransfer reactions that are catalyzed by protein kinases. The protein kinases themselves are typically regulated by phosphorylation and concurrent structural...

Application of NMR in structural proteomics: Screening for proteins amenable to structural analysis (2002)

Rehm, T., Huber, R., Holak, T. A.

In the time of structural proteomics when protein structures are targeted on a genome-wide scale, the detection of "well- behaved" proteins that would yield good quality NMR spectra or X-ray images...

Letter to the editor: Sequence-specific (H-1, N-15, C-13) resonance assignment of the N-terminal domain of the cyclase- associated protein (CAP) from Dictyostelium discoideum (2002)

Rehm, T., Mavoungou, C., Israel, L., Schleicher, M., Holak, T. A.

In silico and NMR identification of inhibitors of the IGF-I and IGF-binding protein-5 interaction (2002)

Kamionka, M., Rehm, T., Beisel, H. G., Lang, K., Engh, R. A., Holak, T. A.

Recently we have determined the crystal structure of the insulin-like growth factor-I (IGF-I) in complex with the N- terminal domain of the IGF-binding protein-5 (IGFBP-5). Here we report results of...

NMR characterization of structure, backbone dynamics, and glutathione binding of the human macrophage migration inhibitory factor (MIF) (1996)

MUHLHAHN, P., BERNHAGEN, J., CZISCH, M., GEORGESCU, J., RENNER, C., ROSS, A., ...

Backbone H-N,N, C alpha and C beta assignment of the GFPuv mutant

Georgescu,J., Rehm,T., Wiehler,J., Steipe,B., Holak,T. A.

31P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3 and (dC-dG)4 in high salt solution.

Holak, T A, Borer, P N, Levy, G C, Van Boom, J H, Wang, A H

In 4M NaCl solutions (dC-dG)n (n = 3,4; approximately 9 mM) exist as a mixture o +/- B and Z forms. The low and high field components of two 31P NMR resonances originating from internal...

Site-directed mutagenesis and structural interpretation of the nidogen binding site of the laminin gamma1 chain.

Pöschl, E, Mayer, U, Stetefeld, J, Baumgartner, R, Holak, T A, Huber, R, ...

A precise molecular map of the nidogen binding site of laminins was obtained by site-directed mutagenesis and structural analysis of the 56 residue LE module gamma1III4 of their gamma1 chain. This...

Structure of the IGF-binding domain of the insulin-like growth factor-binding protein-5 (IGFBP-5): implications for IGF and IGF-I receptor interactions.

Kalus, W, Zweckstetter, M, Renner, C, Sanchez, Y, Georgescu, J, Grol, M, ...

Binding proteins for insulin-like growth factors (IGFs) IGF-I and IGF-II, known as IGFBPs, control the distribution, function and activity of IGFs in various cell tissues and body fluids....

31P-NMR analysis of the B to Z transition in double-stranded (dC-dG)3 and (dC-dG)4 in high salt solution.

Holak, T A, Borer, P N, Levy, G C, Van Boom, J H, Wang, A H

In 4M NaCl solutions (dC-dG)n (n = 3,4; approximately 9 mM) exist as a mixture o +/- B and Z forms. The low and high field components of two 31P NMR resonances originating from internal...

Site-directed mutagenesis and structural interpretation of the nidogen binding site of the laminin gamma1 chain.

Pöschl, E, Mayer, U, Stetefeld, J, Baumgartner, R, Holak, T A, Huber, R, ...

A precise molecular map of the nidogen binding site of laminins was obtained by site-directed mutagenesis and structural analysis of the 56 residue LE module gamma1III4 of their gamma1 chain. This...

Structure of the IGF-binding domain of the insulin-like growth factor-binding protein-5 (IGFBP-5): implications for IGF and IGF-I receptor interactions.

Kalus, W, Zweckstetter, M, Renner, C, Sanchez, Y, Georgescu, J, Grol, M, ...

Binding proteins for insulin-like growth factors (IGFs) IGF-I and IGF-II, known as IGFBPs, control the distribution, function and activity of IGFs in various cell tissues and body fluids....

Backbone H-N,N, C alpha and C beta assignment of the GFPuv mutant

Georgescu, J., Rehm, T., Wiehler, J., Steipe, B., Holak, T. A.

NMR characterization of structure, backbone dynamics, and glutathione binding of the human macrophage migration inhibitory factor (MIF).

Mühlhahn, P., Bernhagen, J., Czisch, M., Georgescu, J., Renner, C., Ross, A., ...

Human macrophage migration inhibitory factor is a 114 amino acid protein that belongs to the family of immunologic cytokines. Assignments of 1H, 15N, and 13C resonances have enabled the determination...

T. A. Holak

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