T. Chakraborty

Details der Publikationsliste

Zeitraum

1998 - 2008

Anzahl

115

Co-Autoren

Broadband electrical detection of spin excitations in Ga0.98Mn0.02As using a photovoltage technique (2008)

Wirthmann, André, Hui, Xiong, Mecking, N., Gui, Y. S., Chakraborty, T., Hu, C. M., ...

We report on microwave photovoltage and simultaneous magnetotransport measurements in a Ga, Mn As film oriented normal to the magnetic field. We detect the ferromagnetic resonance over a broad...

Broadband electrical detection of spin excitations in (Ga,Mn)As using a photovoltage technique (2008)

Wirthmann, André, Hui, Xiong, Mecking, N., Gui, Y. S., Chakraborty, T., Reinwald, M., ...

We report on microwave photovoltage and simultaneous magnetotransport measurements in a (Ga,Mn)As film oriented normal to the magnetic field. We detect the ferromagnetic resonance over a broad...

The heat-shock response of Listeria monocytogenes comprises genes involved in heat shock cell division, cell wall synthesis, and the SOS response (2007)

Hain, T., Wouters, J.A., Hossain, H., Abee, T., ...

The food-borne pathogen Listeria monocytogenes has the ability to survive extreme environmental conditions due to an extensive interacting network of stress responses. It is able to grow and survive...

GECO-linear visualization for comparative genomics (2007)

Kuenne, C. T., Ghai, R., Chakraborty, T., Hain, T.

Summary: In order to understand and interpret phylogenetic and functional relationships between multiple prokaryotic species, qualitative and quantitative data must be correlated and displayed. GECO...

Breaking of Larmor's theorem in quantum Hall states with spin-orbit coupling (2006)

Califano, M., Chakraborty, T., Pietiläinen, P., Hu, C.M.

We investigate the effect of spin-orbit (SO) interaction on the long-wavelength collective spin excitation in a two-dimensional electron gas in the fractional quantum Hall regime. The many-body...

Spin transitions in an incompressible liquid Coulomb-coupled to a quantum dot (2006)

Apalkov, V. M., Chakraborty, T., Schüller, Christian

We report on our investigation of the low- lying energy spectra and charge density of a two-dimensional quantum Hall liquid at v=2/5 that is Coulomb-coupled to a quantum dot. The dot contains a hole...

Active genetic elements present in the locus of enterocyte effacement in Escherichia coli O26 and their role in mobility (2006)

Muniesa, M., Schembri, M. A., Hauf, N., Chakraborty, T.

The locus of enterocyte effacement (LEE) is a large multigene chromosomal segment encoding gene products responsible for the generation of attaching and effacing lesions in many diarrheagenic...

Active genetic elements present in the locus of enterocyte effacement in Escherichia coli O26 and their role in mobility (2006)

Muniesa, M., Schembri, M. A., Hauf, N., Chakraborty, T.

The locus of enterocyte effacement (LEE) is a large multigene chromosomal segment encoding gene products responsible for the generation of attaching and effacing lesions in many diarrheagenic...

Augur--a computational pipeline for whole genome microbial surface protein prediction and classification (2006)

Billion, A., Ghai, R., Chakraborty, T., Hain, T.

Summary: The analysis of protein function is a challenge and a major bottleneck towards well-annotated and analysed microbial genomes. In particular, bacterial surface proteins present an opportunity...

Influence of spin-orbit interaction on quantum cascade transitions (2005)

Apalkov, V., Bagga, A., Chakraborty, T.

We have investigated the effect of spin-orbit (SO) coupling on the emission spectra of a quantum cascade laser. In an externally applied magnetic field parallel to the electron plane, the SO coupling...

Transverse tunneling current through guanine traps in DNA (2005)

Apalkov, V., Chakraborty, T.

The current - voltage dependence of the transverse tunneling current through the electron or hole traps in a DNA is investigated. The hopping of the charge between the sites of the trap and the...

Identification and characterization of di- and tripeptide transporter DtpT of Listeria monocytogenes EGD-e (2005)

Wouters, J.A., Hain, T., Darji, A., Hüfner, E., Wemekamp-Kamphuis, H.H., Chakraborty, T., ...

Listeria monocytogenes is a gram-positive intracellular pathogen responsible for opportunistic infections in humans and animals. Here we identified and characterized the dtpT gene (lmo0555) of L....

Identification of sigma factor sigma(B)-controlled genes and their impact on acid stress, high hydrostatic pressure and freeze survival in Listeria monocytogenes EGD-e (2004)

Wemekamp-Kamphuis, H.H., Wouters, J.A., Hain, T., Chakraborty, T., Abee, T.

The gene encoding the alternative sigma factor B in Listeria monocytogenes is induced upon exposure of cells to several stresses. In this study, we investigated the impact of a sigB null mutation on...

Intersubband magnetophonon resonances in quantum cascade structures (2001)

Smirnov, D., Drachenko, O., Leotin, J., Page, H., Becker, C., Sirtori, C., ...

We report on our magnetotransport measurements of GaAs/GaAlAs quantum cascade structures in a magnetic field of up to 62 T. We observe novel quantum oscillations in tunneling current that are...

Magnetic field induced luminescence spectra in a quantum cascade laser (2000)

Apalkov, V. M., Chakraborty, T.

We report on our study of the luminescence spectra of a quantum cascade laser in the presence of an external magnetic field tilted from the direction perpendicular to the electron plane. The effect...

Theory of the Half-Polarized Quantum Hall States (2000)

Apalkov, V. M., Chakraborty, T., Pietilainen, P., Niemela, K.

We report a theoretical analysis of the half-polarized quantum Hall states observed in a recent experiment. Our numerical results indicate that the ground state energy of the quantum Hall $\nu= 2/3$...

Comment on ``Composite Fermion Hofstadter Problem: Partially Polarized Density Wave States in the $\nu=2/5$ ...'' (2000)

Apalkov, V. M., Chakraborty, T., Pietilainen, P., Niemela, K.

In a recent paper [Phys.Rev.Lett. 82, 3665 (1999)], magnetic field driven spin transitions in fractional quantum Hall (FQH) states were reported, and in particular, at filling factors 2/3 and 2/5,...

Wave Propagation in Laminated Composite Plates, (1998)

Datta, Subhendu K., Shah, Arvind H., Bratton, R. L., Chakraborty, T.

A stiffness method has been used in this paper to study dispersive wave propagation in a laminated anisotropic plate. The advantage of this method is in its usefulness in obtaining numerical results...

Surface and Interface Waves in a Sandwich Plate with Interface Soft Layers, (1998)

Datta, S. K., Shah, A. H., Chakraborty, T., Bratton, R. L.

Dispersion of guided waves in a fiber-reinforced laminated plate with or without soft interface layers between the laminae was studied using an exact method of solution and an approximate stiffness...

Mutagenesis of the myogenin basic region identifies an ancient protein motif critical for activation of myogenesis.

Brennan, T J, Chakraborty, T, Olson, E N

Myogenin is a muscle-specific nuclear factor that acts as a genetic switch to activate myogenesis. Myogenin, MyoD, and a growing number of proteins implicated in transcriptional control share...

A myogenic factor from sea urchin embryos capable of programming muscle differentiation in mammalian cells.

Venuti, J M, Goldberg, L, Chakraborty, T, Olson, E N, Klein, W H

Using the basic helix-loop-helix domain of the myogenic factor myogenin as a probe, we identified a clone from a sea urchin cDNA library with considerable sequence similarity to the vertebrate...

Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes.

Leimeister-Wächter, M, Haffner, C, Domann, E, Goebel, W, Chakraborty, T

We have isolated, by molecular cloning and genetic complementation of a listeriolysin-negative mutant, a gene required for the expression of this virulence factor in Listeria monocytogenes. The...

Interaction of Listeria monocytogenes with mouse dendritic cells.

Guzman, C A, Rohde, M, Chakraborty, T, Domann, E, Hudel, M, Wehland, J, ...

In this study, the interaction of murine dendritic cells with Listeria monocytogenes was investigated. Dendritic cells are efficient antigen-presenting cells, play a key role in the immune response,...

Expression of the Listeria monocytogenes EGD inlA and inlB genes, whose products mediate bacterial entry into tissue culture cell lines, by PrfA-dependent and -independent mechanisms.

Lingnau, A, Domann, E, Hudel, M, Bock, M, Nichterlein, T, Wehland, J, ...

Internalization of Listeria monocytogenes into nonphagocytic cell lines in vitro requires the products of the inlAB locus (J.-L. Gaillard, P. Berche, C. Frehel, E. Gouin, and P. Cossart, Cell...

Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells.

Sibelius, U, Rose, F, Chakraborty, T, Darji, A, Wehland, J, Weiss, S, ...

The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human...

Identification and purification of novel internalin-related proteins in Listeria monocytogenes and Listeria ivanovii.

Lingnau, A, Chakraborty, T, Niebuhr, K, Domann, E, Wehland, J

Monoclonal antibodies were generated against a 30-kDa protein fraction derived from culture supernatants of a Listeria monocytogenes strain complemented with additional copies of the prfA regulator...

The ActA polypeptides of Listeria ivanovii and Listeria monocytogenes harbor related binding sites for host microfilament proteins.

Gerstel, B, Gröbe, L, Pistor, S, Chakraborty, T, Wehland, J

The surface-bound ActA polypeptide of the intracellular bacterial pathogen Listeria monocytogenes acts as a nucleator protein, generating the actin cytoskeleton around intracellularly motile...

Neutralizing monoclonal antibodies against listeriolysin: mapping of epitopes involved in pore formation.

Darji, A, Niebuhr, K, Hense, M, Wehland, J, Chakraborty, T, Weiss, S

Six different mouse monoclonal antibodies (MAbs) and a specific rabbit polygonal antibody were raised against listeriolysin. Four of the MAbs also recognized seeligeriolysin, and five cross-reacted...

Temperature- and medium-dependent secretion of proteins by Shiga toxin-producing Escherichia coli.

Ebel, F, Deibel, C, Kresse, A U, Guzmán, C A, Chakraborty, T

Infections due to Shiga toxin-producing Escherichia coli (STEC) are responsible for severe diarrheal disease in humans and livestock, and these bacteria have recently emerged as a leading cause of...

Identification and characterization of a novel PrfA-regulated gene in Listeria monocytogenes whose product, IrpA, is highly homologous to internalin proteins, which contain leucine-rich repeats.

Domann, E, Zechel, S, Lingnau, A, Hain, T, Darji, A, Nichterlein, T, ...

The expression of all virulence factors in Listeria monocytogenes characterized to date is controlled by the virulence regulator protein, PrfA. To identify further PrfA-regulated proteins, we...

Listeriolysin and IrpA are major protein targets of the human humoral response against Listeria monocytogenes.

Grenningloh, R, Darji, A, Wehland, J, Chakraborty, T, Weiss, S

We have examined the human humoral immune response directed against proteins of Listeria monocytogenes in both healthy individuals and listeriosis patients. Two major targets for an antibody response...

Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.

Schäferkordt, S, Chakraborty, T

The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz,...

Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species.

Notermans, S H, Dufrenne, J, Leimeister-Wächter, M, Domann, E, Chakraborty, T

In this study, 468 Listeria strains were checked for the presence of phosphatidylinositol-specific phospholipase C (PI-PLC) activity by using a simple assay that consisted of overlaying colonies...

Specific gene probe for detection of biotyped and serotyped Listeria strains.

Notermans, S, Chakraborty, T, Leimeister-Wächter, M, Dufrenne, J, Heuvelman, K J, Maas, H, ...

A total of 284 strains of Listeria, including all known serovars and biovars together with Listeria grayi and Listeria murrayi, were biotyped and serotyped. Biotyping and serotyping could be done in...

Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp.

Wernars, K, Heuvelman, K, Notermans, S, Domann, E, Leimeister-Wächter, M, Chakraborty, T

The pathogenesis of listerial infections is complex and involves a number of virulence factors expressed by virulent Listeria species. We have recently described a regulator gene, prfA, that...

Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene.

Chakraborty, T, Leimeister-Wächter, M, Domann, E, Hartl, M, Goebel, W, Nichterlein, T, ...

The prfA gene of Listeria monocytogenes encodes a protein that activates transcription of the listeriolysin gene (lisA). In order to explore the role of the prfA gene product in the pathogenesis of...

The expression of virulence genes in Listeria monocytogenes is thermoregulated.

Leimeister-Wächter, M, Domann, E, Chakraborty, T

The expression of listeriolysin, a major virulence factor of the gram-positive facultative intracellular pathogen Listeria monocytogenes, is positively regulated by a transcriptional activator, the...

Cloning, expression, and mapping of the Aeromonas hydrophila aerolysin gene determinant in Escherichia coli K-12.

Chakraborty, T, Huhle, B, Bergbauer, H, Goebel, W

DNA sequences corresponding to the aerolysin gene (aer) of Aeromonas hydrophila AH2 DNA were identified by screening a cosmid gene library for hemolytic and cytotoxic activities. A plasmid containing...

Molecular cloning, sequencing, and identification of a metalloprotease gene from Listeria monocytogenes that is species specific and physically linked to the listeriolysin gene.

Domann, E, Leimeister-Wächter, M, Goebel, W, Chakraborty, T

The entire nucleotide sequence of an open reading frame located immediately downstream of the listeriolysin gene from a virulent Listeria monocytogenes serotype 1/2a strain was determined. The...

The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes.

Köhler, S, Leimeister-Wächter, M, Chakraborty, T, Lottspeich, F, Goebel, W

The gene of Listeria monocytogenes that encodes a major extracellular protein (p60) was cloned in Escherichia coli. The gene was designated iap, as p60 was previously shown to represent an...

Aerolysin of Aeromonas sobria: evidence for formation of ion-permeable channels and comparison with alpha-toxin of Staphylococcus aureus.

Chakraborty, T, Schmid, A, Notermans, S, Benz, R

Aerolysin from Aeromonas sobria AB3 was isolated and purified. The pure toxin formed sodium dodecyl sulfate-insoluble oligomers in a lipidic environment. The addition of aerolysin to the aqueous...

Marker exchange mutagenesis of the aerolysin determinant in Aeromonas hydrophila demonstrates the role of aerolysin in A. hydrophila-associated systemic infections.

Chakraborty, T, Huhle, B, Hof, H, Bergbauer, H, Goebel, W

We report here on the isolation of isogenic strains of Aeromonas hydrophila AB3 deleted for a segment of the aerolysin gene. All aer mutants obtained lacked the 49-kilodalton aerolysin gene product...

Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.

Chakraborty, T, Montenegro, M A, Sanyal, S C, Helmuth, R, Bulling, E, Timmis, K N

Culture filtrates of two Aeromonas hydrophila strains which were isolated from patients with diarrhea and assumed to be causative agents of the infections were shown to contain enterotoxic,...

Pseudoappendicitis caused by Plesiomonas shigelloides.

Fischer, K, Chakraborty, T, Hof, H, Kirchner, T, Wamsler, O

A 20-year-old patient was hospitalized with clinical signs of acute appendicitis. After surgery, the histological findings in the appendix and a lymphatic node suggested the diagnosis of...

Aeromonas trota sp. nov., an ampicillin-susceptible species isolated from clinical specimens.

Carnahan, A M, Chakraborty, T, Fanning, G R, Verma, D, Ali, A, Janda, J M, ...

Previous DNA hybridization studies established 12 Aeromonas genospecies, from which nine phenotypic species have been proposed: Aeromonas hydrophila, A. sobria, A. caviae, A. media, A. veronii, A....

Localization of the ActA polypeptide of Listeria monocytogenes in infected tissue culture cell lines: ActA is not associated with actin "comets".

Niebuhr, K, Chakraborty, T, Rohde, M, Gazlig, T, Jansen, B, Köllner, P, ...

The ActA protein of the gram-positive pathogen Listeria monocytogenes is a 90-kDa polypeptide required for interaction of the bacteria with components of the host cell microfilament system to...

Detection of a prfA-independent promoter responsible for listeriolysin gene expression in mutant Listeria monocytogenes strains lacking the PrfA regulator.

Domann, E, Wehland, J, Niebuhr, K, Haffner, C, Leimeister-Wächter, M, Chakraborty, T

Expression of listeriolysin, a major virulence factor of pathogenic Listeria monocytogenes, is positively regulated by the pleiotropic virulence regulator PrfA. In this study, we demonstrate that L....

Mammalian cells transfected with the listeriolysin gene exhibit enhanced proliferation and focus formation.

Demuth, A, Chakraborty, T, Krohne, G, Goebel, W

Mouse 3T6 and 3T3 fibroblasts and rat epithelial L2 cells were transfected with recombinant plasmids containing the listeriolysin gene (hly) of Listeria monocytogenes. This bacterial gene (with and...

Detection of listeriolysin, the thiol-dependent hemolysin in Listeria monocytogenes, Listeria ivanovii, and Listeria seeligeri.

Leimeister-Wächter, M, Chakraborty, T

The listeriolysin gene from a weakly hemolytic but virulent strain of Listeria monocytogenes serotype 1/2a was cloned in Escherichia coli K-12. Recombinants were identified on the basis of their...

Analysis of the myogenin promoter reveals an indirect pathway for positive autoregulation mediated by the muscle-specific enhancer factor MEF-2.

Edmondson, D G, Cheng, T C, Cserjesi, P, Chakraborty, T, Olson, E N

Transcriptional cascades that specify cell fate have been well described in invertebrates. In mammalian development, however, gene hierarchies involved in determination of cell lineage are not...

Inefficient homooligomerization contributes to the dependence of myogenin on E2A products for efficient DNA binding.

Chakraborty, T, Brennan, T J, Li, L, Edmondson, D, Olson, E N

Myogenin is a muscle-specific transcription factor that can activate myogenesis; it belongs to a family of transcription factors that share homology within a basic region and an adjacent...

Domains outside of the DNA-binding domain impart target gene specificity to myogenin and MRF4.

Chakraborty, T, Olson, E N

Myogenin and MRF4 belong to the MyoD family of muscle-specific transcription factors, which can activate myogenesis when introduced into nonmyogenic cells. These proteins share homology within a...

Identification of HeLa cell nuclear factors that bind to and activate the early promoter of human polyomavirus BK in vitro.

Chakraborty, T, Das, G C

Human polyomavirus BK (BKV), an oncogenic DNA virus, differs from other papovaviruses in the organization of the regulatory region and in tissue tropism for kidney cells. The noncoding regulatory...

The basic region of myogenin cooperates with two transcription activation domains to induce muscle-specific transcription.

Schwarz, J J, Chakraborty, T, Martin, J, Zhou, J M, Olson, E N

Myogenin is a skeletal muscle-specific transcription factor that can activate myogenesis when introduced into a variety of nonmuscle cell types. Activation of the myogenic program by myogenin is...

The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton.

Pistor, S, Chakraborty, T, Niebuhr, K, Domann, E, Wehland, J

Listeria monocytogenes, a facultative intracellular pathogen, employs actin and other microfilament-associated proteins to move through the host cell cytoplasm. Isogenic mutants of L. monocytogenes...

A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells.

Chakraborty, T, Ebel, F, Domann, E, Niebuhr, K, Gerstel, B, Pistor, S, ...

The surface-bound ActA polypeptide of the intracellular bacterial pathogen Listeria monocytogenes is the sole listerial factor needed for recruitment of host actin filaments by intracellularly motile...

Aeromonas hydrophila Beta-Hemolysin Induces Active Chloride Secretion in Colon Epithelial Cells (HT-29/B6)

Epple, H. J., Mankertz, J., Ignatius, R., Liesenfeld, O., Fromm, M., Zeitz, M., ...

The diarrheal mechanisms in Aeromonas enteritis are not completely understood. In this study we investigated the effect of aeromonads and of their secretory products on ion secretion and barrier...

Purification of the E. coli dnaA gene product.

Chakraborty, T, Yoshinaga, K, Lother, H, Messer, W

The product of the dnaA gene of Escherichia coli was isolated in a highly enriched form. The purification product binds specifically to DNA containing the E. coli chromosomal origin of replication,...

A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin.

Domann, E, Wehland, J, Rohde, M, Pistor, S, Hartl, M, Goebel, W, ...

The ability of Listeria monocytogenes to move within the cytosol of infected cells and their ability to infect adjacent cells is important in the development of infection foci leading to systemic...

A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family.

Niebuhr, K, Ebel, F, Frank, R, Reinhard, M, Domann, E, Carl, U D, ...

The ActA protein of the intracellular pathogen Listeria monocytogenes induces a dramatic reorganization of the actin-based cytoskeleton. Two profilin binding proteins, VASP and Mena, are the only...

Mutagenesis of the myogenin basic region identifies an ancient protein motif critical for activation of myogenesis.

Brennan, T J, Chakraborty, T, Olson, E N

Myogenin is a muscle-specific nuclear factor that acts as a genetic switch to activate myogenesis. Myogenin, MyoD, and a growing number of proteins implicated in transcriptional control share...

A myogenic factor from sea urchin embryos capable of programming muscle differentiation in mammalian cells.

Venuti, J M, Goldberg, L, Chakraborty, T, Olson, E N, Klein, W H

Using the basic helix-loop-helix domain of the myogenic factor myogenin as a probe, we identified a clone from a sea urchin cDNA library with considerable sequence similarity to the vertebrate...

Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes.

Leimeister-Wächter, M, Haffner, C, Domann, E, Goebel, W, Chakraborty, T

We have isolated, by molecular cloning and genetic complementation of a listeriolysin-negative mutant, a gene required for the expression of this virulence factor in Listeria monocytogenes. The...

Interaction of Listeria monocytogenes with mouse dendritic cells.

Guzman, C A, Rohde, M, Chakraborty, T, Domann, E, Hudel, M, Wehland, J, ...

In this study, the interaction of murine dendritic cells with Listeria monocytogenes was investigated. Dendritic cells are efficient antigen-presenting cells, play a key role in the immune response,...

Expression of the Listeria monocytogenes EGD inlA and inlB genes, whose products mediate bacterial entry into tissue culture cell lines, by PrfA-dependent and -independent mechanisms.

Lingnau, A, Domann, E, Hudel, M, Bock, M, Nichterlein, T, Wehland, J, ...

Internalization of Listeria monocytogenes into nonphagocytic cell lines in vitro requires the products of the inlAB locus (J.-L. Gaillard, P. Berche, C. Frehel, E. Gouin, and P. Cossart, Cell...

Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells.

Sibelius, U, Rose, F, Chakraborty, T, Darji, A, Wehland, J, Weiss, S, ...

The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human...

Identification and purification of novel internalin-related proteins in Listeria monocytogenes and Listeria ivanovii.

Lingnau, A, Chakraborty, T, Niebuhr, K, Domann, E, Wehland, J

Monoclonal antibodies were generated against a 30-kDa protein fraction derived from culture supernatants of a Listeria monocytogenes strain complemented with additional copies of the prfA regulator...

The ActA polypeptides of Listeria ivanovii and Listeria monocytogenes harbor related binding sites for host microfilament proteins.

Gerstel, B, Gröbe, L, Pistor, S, Chakraborty, T, Wehland, J

The surface-bound ActA polypeptide of the intracellular bacterial pathogen Listeria monocytogenes acts as a nucleator protein, generating the actin cytoskeleton around intracellularly motile...

Neutralizing monoclonal antibodies against listeriolysin: mapping of epitopes involved in pore formation.

Darji, A, Niebuhr, K, Hense, M, Wehland, J, Chakraborty, T, Weiss, S

Six different mouse monoclonal antibodies (MAbs) and a specific rabbit polygonal antibody were raised against listeriolysin. Four of the MAbs also recognized seeligeriolysin, and five cross-reacted...

Temperature- and medium-dependent secretion of proteins by Shiga toxin-producing Escherichia coli.

Ebel, F, Deibel, C, Kresse, A U, Guzmán, C A, Chakraborty, T

Infections due to Shiga toxin-producing Escherichia coli (STEC) are responsible for severe diarrheal disease in humans and livestock, and these bacteria have recently emerged as a leading cause of...

Identification and characterization of a novel PrfA-regulated gene in Listeria monocytogenes whose product, IrpA, is highly homologous to internalin proteins, which contain leucine-rich repeats.

Domann, E, Zechel, S, Lingnau, A, Hain, T, Darji, A, Nichterlein, T, ...

The expression of all virulence factors in Listeria monocytogenes characterized to date is controlled by the virulence regulator protein, PrfA. To identify further PrfA-regulated proteins, we...

Listeriolysin and IrpA are major protein targets of the human humoral response against Listeria monocytogenes.

Grenningloh, R, Darji, A, Wehland, J, Chakraborty, T, Weiss, S

We have examined the human humoral immune response directed against proteins of Listeria monocytogenes in both healthy individuals and listeriosis patients. Two major targets for an antibody response...

Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.

Schäferkordt, S, Chakraborty, T

The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz,...

Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species.

Notermans, S H, Dufrenne, J, Leimeister-Wächter, M, Domann, E, Chakraborty, T

In this study, 468 Listeria strains were checked for the presence of phosphatidylinositol-specific phospholipase C (PI-PLC) activity by using a simple assay that consisted of overlaying colonies...

Specific gene probe for detection of biotyped and serotyped Listeria strains.

Notermans, S, Chakraborty, T, Leimeister-Wächter, M, Dufrenne, J, Heuvelman, K J, Maas, H, ...

A total of 284 strains of Listeria, including all known serovars and biovars together with Listeria grayi and Listeria murrayi, were biotyped and serotyped. Biotyping and serotyping could be done in...

Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp.

Wernars, K, Heuvelman, K, Notermans, S, Domann, E, Leimeister-Wächter, M, Chakraborty, T

The pathogenesis of listerial infections is complex and involves a number of virulence factors expressed by virulent Listeria species. We have recently described a regulator gene, prfA, that...

Coordinate regulation of virulence genes in Listeria monocytogenes requires the product of the prfA gene.

Chakraborty, T, Leimeister-Wächter, M, Domann, E, Hartl, M, Goebel, W, Nichterlein, T, ...

The prfA gene of Listeria monocytogenes encodes a protein that activates transcription of the listeriolysin gene (lisA). In order to explore the role of the prfA gene product in the pathogenesis of...

The expression of virulence genes in Listeria monocytogenes is thermoregulated.

Leimeister-Wächter, M, Domann, E, Chakraborty, T

The expression of listeriolysin, a major virulence factor of the gram-positive facultative intracellular pathogen Listeria monocytogenes, is positively regulated by a transcriptional activator, the...

Cloning, expression, and mapping of the Aeromonas hydrophila aerolysin gene determinant in Escherichia coli K-12.

Chakraborty, T, Huhle, B, Bergbauer, H, Goebel, W

DNA sequences corresponding to the aerolysin gene (aer) of Aeromonas hydrophila AH2 DNA were identified by screening a cosmid gene library for hemolytic and cytotoxic activities. A plasmid containing...

Molecular cloning, sequencing, and identification of a metalloprotease gene from Listeria monocytogenes that is species specific and physically linked to the listeriolysin gene.

Domann, E, Leimeister-Wächter, M, Goebel, W, Chakraborty, T

The entire nucleotide sequence of an open reading frame located immediately downstream of the listeriolysin gene from a virulent Listeria monocytogenes serotype 1/2a strain was determined. The...

The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes.

Köhler, S, Leimeister-Wächter, M, Chakraborty, T, Lottspeich, F, Goebel, W

The gene of Listeria monocytogenes that encodes a major extracellular protein (p60) was cloned in Escherichia coli. The gene was designated iap, as p60 was previously shown to represent an...

Aerolysin of Aeromonas sobria: evidence for formation of ion-permeable channels and comparison with alpha-toxin of Staphylococcus aureus.

Chakraborty, T, Schmid, A, Notermans, S, Benz, R

Aerolysin from Aeromonas sobria AB3 was isolated and purified. The pure toxin formed sodium dodecyl sulfate-insoluble oligomers in a lipidic environment. The addition of aerolysin to the aqueous...

Marker exchange mutagenesis of the aerolysin determinant in Aeromonas hydrophila demonstrates the role of aerolysin in A. hydrophila-associated systemic infections.

Chakraborty, T, Huhle, B, Hof, H, Bergbauer, H, Goebel, W

We report here on the isolation of isogenic strains of Aeromonas hydrophila AB3 deleted for a segment of the aerolysin gene. All aer mutants obtained lacked the 49-kilodalton aerolysin gene product...

Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin.

Chakraborty, T, Montenegro, M A, Sanyal, S C, Helmuth, R, Bulling, E, Timmis, K N

Culture filtrates of two Aeromonas hydrophila strains which were isolated from patients with diarrhea and assumed to be causative agents of the infections were shown to contain enterotoxic,...

Pseudoappendicitis caused by Plesiomonas shigelloides.

Fischer, K, Chakraborty, T, Hof, H, Kirchner, T, Wamsler, O

A 20-year-old patient was hospitalized with clinical signs of acute appendicitis. After surgery, the histological findings in the appendix and a lymphatic node suggested the diagnosis of...

Aeromonas trota sp. nov., an ampicillin-susceptible species isolated from clinical specimens.

Carnahan, A M, Chakraborty, T, Fanning, G R, Verma, D, Ali, A, Janda, J M, ...

Previous DNA hybridization studies established 12 Aeromonas genospecies, from which nine phenotypic species have been proposed: Aeromonas hydrophila, A. sobria, A. caviae, A. media, A. veronii, A....

Localization of the ActA polypeptide of Listeria monocytogenes in infected tissue culture cell lines: ActA is not associated with actin "comets".

Niebuhr, K, Chakraborty, T, Rohde, M, Gazlig, T, Jansen, B, Köllner, P, ...

The ActA protein of the gram-positive pathogen Listeria monocytogenes is a 90-kDa polypeptide required for interaction of the bacteria with components of the host cell microfilament system to...

Detection of a prfA-independent promoter responsible for listeriolysin gene expression in mutant Listeria monocytogenes strains lacking the PrfA regulator.

Domann, E, Wehland, J, Niebuhr, K, Haffner, C, Leimeister-Wächter, M, Chakraborty, T

Expression of listeriolysin, a major virulence factor of pathogenic Listeria monocytogenes, is positively regulated by the pleiotropic virulence regulator PrfA. In this study, we demonstrate that L....

Mammalian cells transfected with the listeriolysin gene exhibit enhanced proliferation and focus formation.

Demuth, A, Chakraborty, T, Krohne, G, Goebel, W

Mouse 3T6 and 3T3 fibroblasts and rat epithelial L2 cells were transfected with recombinant plasmids containing the listeriolysin gene (hly) of Listeria monocytogenes. This bacterial gene (with and...

Detection of listeriolysin, the thiol-dependent hemolysin in Listeria monocytogenes, Listeria ivanovii, and Listeria seeligeri.

Leimeister-Wächter, M, Chakraborty, T

The listeriolysin gene from a weakly hemolytic but virulent strain of Listeria monocytogenes serotype 1/2a was cloned in Escherichia coli K-12. Recombinants were identified on the basis of their...

Analysis of the myogenin promoter reveals an indirect pathway for positive autoregulation mediated by the muscle-specific enhancer factor MEF-2.

Edmondson, D G, Cheng, T C, Cserjesi, P, Chakraborty, T, Olson, E N

Transcriptional cascades that specify cell fate have been well described in invertebrates. In mammalian development, however, gene hierarchies involved in determination of cell lineage are not...

Inefficient homooligomerization contributes to the dependence of myogenin on E2A products for efficient DNA binding.

Chakraborty, T, Brennan, T J, Li, L, Edmondson, D, Olson, E N

Myogenin is a muscle-specific transcription factor that can activate myogenesis; it belongs to a family of transcription factors that share homology within a basic region and an adjacent...

Domains outside of the DNA-binding domain impart target gene specificity to myogenin and MRF4.

Chakraborty, T, Olson, E N

Myogenin and MRF4 belong to the MyoD family of muscle-specific transcription factors, which can activate myogenesis when introduced into nonmyogenic cells. These proteins share homology within a...

Identification of HeLa cell nuclear factors that bind to and activate the early promoter of human polyomavirus BK in vitro.

Chakraborty, T, Das, G C

Human polyomavirus BK (BKV), an oncogenic DNA virus, differs from other papovaviruses in the organization of the regulatory region and in tissue tropism for kidney cells. The noncoding regulatory...

The basic region of myogenin cooperates with two transcription activation domains to induce muscle-specific transcription.

Schwarz, J J, Chakraborty, T, Martin, J, Zhou, J M, Olson, E N

Myogenin is a skeletal muscle-specific transcription factor that can activate myogenesis when introduced into a variety of nonmuscle cell types. Activation of the myogenic program by myogenin is...

The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton.

Pistor, S, Chakraborty, T, Niebuhr, K, Domann, E, Wehland, J

Listeria monocytogenes, a facultative intracellular pathogen, employs actin and other microfilament-associated proteins to move through the host cell cytoplasm. Isogenic mutants of L. monocytogenes...

A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells.

Chakraborty, T, Ebel, F, Domann, E, Niebuhr, K, Gerstel, B, Pistor, S, ...

The surface-bound ActA polypeptide of the intracellular bacterial pathogen Listeria monocytogenes is the sole listerial factor needed for recruitment of host actin filaments by intracellularly motile...

Aeromonas hydrophila Beta-Hemolysin Induces Active Chloride Secretion in Colon Epithelial Cells (HT-29/B6)

Epple, H. J., Mankertz, J., Ignatius, R., Liesenfeld, O., Fromm, M., Zeitz, M., ...

The diarrheal mechanisms in Aeromonas enteritis are not completely understood. In this study we investigated the effect of aeromonads and of their secretory products on ion secretion and barrier...

Purification of the E. coli dnaA gene product.

Chakraborty, T, Yoshinaga, K, Lother, H, Messer, W

The product of the dnaA gene of Escherichia coli was isolated in a highly enriched form. The purification product binds specifically to DNA containing the E. coli chromosomal origin of replication,...

A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin.

Domann, E, Wehland, J, Rohde, M, Pistor, S, Hartl, M, Goebel, W, ...

The ability of Listeria monocytogenes to move within the cytosol of infected cells and their ability to infect adjacent cells is important in the development of infection foci leading to systemic...

A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family.

Niebuhr, K, Ebel, F, Frank, R, Reinhard, M, Domann, E, Carl, U D, ...

The ActA protein of the intracellular pathogen Listeria monocytogenes induces a dramatic reorganization of the actin-based cytoskeleton. Two profilin binding proteins, VASP and Mena, are the only...

Active genetic elements present in the locus of enterocyte effacement in Escherichia coli O26 and their role in mobility

Muniesa, M, Schembri, MA, Hauf, N, Chakraborty, T

The locus of enterocyte effacement (LEE) is a large multigene chromosomal segment encoding gene products responsible for the generation of attaching and effacing lesions in many diarrheagenic...