T. D. Warner

Effects of non-steroidal anti-inflammatory drugs on cyclo-oxygenase and lipoxygenase activity in whole blood from aspirin-sensitive asthmatics vs healthy donors (2002)

P A Gray, T D Warner, I Vojnovic, P Del Soldato, A Parikh, G K Scadding, ...

Placentally derived prostaglandin E2 acts via the EP4 receptor to inhibit IL-2-dependent proliferation of CTLL-2 T cells (2002)

Kvirkvelia, N., Vojnovic, I., Warner, T.D., Athie-Morales, V., Free, P., Rayment, N., ...

A number of immunomodulatory molecules are present in the placenta, including cytokines, prostaglandins, progesterone and indoleamine 2,3-dioxygenase. An undefined factor capable of down-regulating...

Placentally derived prostaglandin E2 acts via the EP4 receptor to inhibit IL-2-dependent proliferation of CTLL-2 T cells. (2002)

Kirkvelia , N., Vojnovic, I., Warner, T. D., Athie-Morales, V., Free, P., Rayment, N., ...

Placentally derived prostaglandin E2 acts via the EP4 receptor to inhibit IL-2-dependent proliferation of CTLL-2 T cells. (2002)

Kvirkvelia , N., Vojnovic, I., Warner, T. D., Athie-Morales, V., Free, P., Rayment, N., ...

A number of immunomodulatory molecules are present in the placenta, including cytokines, prostaglandins, progesterone and indoleamine 2,3-dioxygenase. An undefined factor capable of down-regulating...

Placentally-derived prostoglandin E2 acts via the EP4 receptor to inhibit IL-2-dependent proliferation of cytotoxic T cells. (2002)

Kvirkvelia , N., Vojnovic, I., Warner, T. D., Athie-Morales, V., Free, P., Raymont, N., ...

A number of immunomodulatory molecules are present in the placenta, including cytokines, prostaglandins, progesterone and indoleamine 2,3-dioxygenase. An undefined factor capable of down-regulating...

Placentally derived prostaglandin E2 acts via the EP4 receptor to inhibit IL-2-dependent proliferation of CTLL-2 T cells. (2002)

Kvirkvelia, N., Vojnovic, I., Warner, T. D., Athie-Morales, V., Free, P., Rayment, N., ...

A number of immunomodulatory molecules are present in the placenta, including cytokines, prostaglandins, progesterone and indoleamine 2,3-dioxygenase. An undefined factor capable of down-regulating...

Endothelin 1 mediates ex vivo coronary vasoconstriction caused by exogenous and endogenous cytokines.

Klemm, P, Warner, T D, Hohlfeld, T, Corder, R, Vane, J R

Treatment of rats with cytokines has been associated with an increase in the circulating levels of endothelin 1 (ET-1). Here we show that administration of tumor necrosis factor alpha (TNF-alpha; 4...

Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.

Pollock, J S, Förstermann, U, Mitchell, J A, Warner, T D, Schmidt, H H, Nakane, M, ...

The particulate enzyme responsible for the synthesis of endothelium-derived relaxing factor has been purified from cultured and native (noncultured) bovine aortic endothelial cells. Purification of...

Receptor-mediated release of endothelium-derived relaxing factor and prostacyclin from bovine aortic endothelial cells is coupled.

De Nucci, G, Gryglewski, R J, Warner, T D, Vane, J R

Bovine aortic endothelial cells were grown on microcarrier beads and were perfused with Krebs-Ringer solution. Endothelium-derived relaxing factor (EDRF) was bioassayed on a cascade of four strips of...

Pressor effects of circulating endothelin are limited by its removal in the pulmonary circulation and by the release of prostacyclin and endothelium-derived relaxing factor.

De Nucci, G, Thomas, R, D'Orleans-Juste, P, Antunes, E, Walder, C, Warner, T D, ...

Endothelin releases prostacyclin and thromboxane A2 from guinea pig or rat isolated lungs and endothelium-derived relaxing factor in the perfused mesentery of the rat. Endothelin is also...

Endothelin 1 mediates ex vivo coronary vasoconstriction caused by exogenous and endogenous cytokines.

Klemm, P, Warner, T D, Hohlfeld, T, Corder, R, Vane, J R

Treatment of rats with cytokines has been associated with an increase in the circulating levels of endothelin 1 (ET-1). Here we show that administration of tumor necrosis factor alpha (TNF-alpha; 4...

Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.

Pollock, J S, Förstermann, U, Mitchell, J A, Warner, T D, Schmidt, H H, Nakane, M, ...

The particulate enzyme responsible for the synthesis of endothelium-derived relaxing factor has been purified from cultured and native (noncultured) bovine aortic endothelial cells. Purification of...

Receptor-mediated release of endothelium-derived relaxing factor and prostacyclin from bovine aortic endothelial cells is coupled.

De Nucci, G, Gryglewski, R J, Warner, T D, Vane, J R

Bovine aortic endothelial cells were grown on microcarrier beads and were perfused with Krebs-Ringer solution. Endothelium-derived relaxing factor (EDRF) was bioassayed on a cascade of four strips of...

Pressor effects of circulating endothelin are limited by its removal in the pulmonary circulation and by the release of prostacyclin and endothelium-derived relaxing factor.

De Nucci, G, Thomas, R, D'Orleans-Juste, P, Antunes, E, Walder, C, Warner, T D, ...

Endothelin releases prostacyclin and thromboxane A2 from guinea pig or rat isolated lungs and endothelium-derived relaxing factor in the perfused mesentery of the rat. Endothelin is also...

Placentally derived prostaglandin E2 acts via the EP4 receptor to inhibit IL-2-dependent proliferation of CTLL-2 T cells

KVIRKVELIA, N, VOJNOVIC, I, WARNER, T D, ATHIE-MORALES, V, FREE, P, RAYMENT, N, ...

A number of immunomodulatory molecules are present in the placenta, including cytokines, prostaglandins, progesterone and indoleamine 2,3-dioxygenase. An undefined factor capable of down-regulating...

Rapid degradation of endothelin-1 by an enzyme released by the rat isolated perfused mesentery.

Pérez-Vizcaíno, F, Cooper, A C, Corder, R, Fournier, A, Warner, T D

1. In vivo the effects of endothelin-1 (ET-1) are limited by its rapid removal from the circulation and possibly by its metabolism by enzymes such as neutral endopeptidase 24.11, deamidase or...

Co-induction of nitric oxide synthase and cyclo-oxygenase: interactions between nitric oxide and prostanoids.

Swierkosz, T A, Mitchell, J A, Warner, T D, Botting, R M, Vane, J R

1. Lipopolysaccharide (LPS) co-induces nitric oxide synthase (iNOS) and cyclo-oxygenase (COX-2) in J774.2 macrophages. Here we have used LPS-activated J774.2 macrophages to investigate the effects of...

Coronary vasoconstriction in vitro in the hearts of polyarthritic rats: effectiveness of in vivo treatment with the endothelin receptor antagonist SB 209670.

Klemm, P, Warner, T D, Willis, D, Moore, A R, Vane, J R

Here we demonstrate that perfused hearts removed from polyarthritic rats develop a pronounced coronary vasoconstriction ex vivo. This vasoconstriction is almost entirely blocked by in vivo...

Contractile activity of endothelin precursors in the isolated gallbladder of the guinea-pig: presence of an endothelin-converting enzyme.

Battistini, B, Woods, M, O'Donnell, L J, Warner, T D, Corder, R, Fournier, A, ...

1. We have compared the activities of the endothelin precursors (human big ET-1(1-38), porcine big ET-1(1-39), big ET-2(1-38) and big ET-3(1-41 amide) and their respective mature 21 amino acid...

Comparison of effects of chronic and acute administration of NG-nitro-L-arginine methyl ester to the rat on inhibition of nitric oxide-mediated responses.

Bryant, C E, Allcock, G H, Warner, T D

1. Vascular responses to acetylcholine and sodium nitroprusside in vivo and in vitro, in the isolated perfused kidney and in rings of rat thoracic aorta, were measured in rats treated chronically...

Nitric oxide, and not vasoactive intestinal peptide, as the main neurotransmitter of vagally induced relaxation of the guinea pig stomach.

Desai, K M, Warner, T D, Bishop, A E, Polak, J M, Vane, J R

1. Nitric oxide synthase (NOS) was localized in the guinea pig stomach by immunocytochemistry. In vitro experiments were carried out on the isolated stomach of the guinea pig to study any possible...

Effects of non-steroidal anti-inflammatory drugs on cyclo-oxygenase and lipoxygenase activity in whole blood from aspirin-sensitive asthmatics vs healthy donors

Gray, P A, Warner, T D, Vojnovic, I, Del Soldato, P, Parikh, A, Scadding, G K, ...

Cyclo-oxygenase (COX) and lipoxygenase (LO) share a common substrate, arachidonic acid. Aspirin and related drugs inhibit COX activity. In a subset of patients with asthma aspirin induces clinical...

Comparison of the survival of endothelium-derived relaxing factor and nitric oxide within the isolated perfused mesenteric arterial bed of the rat.

Warner, T. D., De Nucci, G., Vane, J. R.

1. The survival of the endothelium-derived relaxing factor (EDRF) released by acetylcholine (ACh) in the rat isolated perfused mesenteric vascular bed was compared to that of exogenously applied...

Human brain contains a metalloprotease that converts big endothelin-1 to endothelin-1 and is inhibited by phosphoramidon and EDTA.

Warner, T. D., Schmidt, H. H., Kuk, J., Mitchell, J. A., Murad, F.

Incubation of big endothelin-1 (bET-1) with protein derived from the detergent-extracted 100,000 g pellet prepared from human brain tissue resulted in the formation of endothelin-1 (ET-1) at a rate...

Regional differences in endothelin converting enzyme activity in rat brain: inhibition by phosphoramidon and EDTA.

Warner, T. D., Budzik, G. P., Matsumoto, T., Mitchell, J. A., Förstermann, U., Murad, F.

1. It has been demonstrated previously that conversion of big endothelin-1 (bET-1) to endothelin-1 (ET-1) is inhibited in vitro and in vivo by phosphoramidon. In addition, ET-1 binding sites and mRNA...

Nitric oxide synthase in ferret brain: localization and characterization.

Matsumoto, T., Mitchell, J. A., Schmidt, H. H., Kohlhaas, K. L., Warner, T. D., Förstermann, U., ...

1. In the present study, we have investigated the distribution of nitric oxide synthase in the ferret brain. Nitric oxide synthase was determined biochemically and immunochemically. 2. In the rat...

Mediation via different receptors of the vasoconstrictor effects of endothelins and sarafotoxins in the systemic circulation and renal vasculature of the anaesthetized rat.

Cristol, J. P., Warner, T. D., Thiemermann, C., Vane, J. R.

1. Using endothelin-1 (ET-1), endothelin-3 (ET-3), sarafotoxin 6b (SX6b) and sarafotoxin 6c (SX6c) as agonists and BQ-123 as a selective ETA receptor antagonist, we have examined the endothelin...

Alkaline buffers release EDRF from bovine cultured aortic endothelial cells.

Mitchell, J. A., De Nucci, G., Warner, T. D., Vane, J. R.

1. Release of endothelium-derived relaxing factor (EDRF) and prostacyclin (PGI2) from bovine cultured aortic endothelial cells (EC) was measured by bioassay and radioimmunoassay, respectively. 2....

Different patterns of release of endothelium-derived relaxing factor and prostacyclin.

Mitchell, J. A., De Nucci, G., Warner, T. D., Vane, J. R.

1. Release of endothelium derived relaxing factor (EDRF) and prostacyclin (PGI2) from endothelial cells (EC) cultured from bovine aortae was measured by bioassay and radioimmunoassay, respectively,...

Characterization of receptors for kinins and neurokinins in the arterial and venous mesenteric vasculatures of the guinea-pig.

Berthiaume, N., Claing, A., Regoli, D., Warner, T. D., D'Orléans-Juste, P.

1. In the present work, we have studied the microvascular reactivity of the arterial and venous mesenteric beds of the guinea-pig to bradykinin, neurokinins and other agents. 2. The vasoactive...

Roles of endothelin receptors in the regional and systemic vascular responses to ET-1 in the anaesthetized ganglion-blocked rat: use of selective antagonists.

Allcock, G. H., Warner, T. D., Vane, J. R.

1. Endothelin-1 (ET-1) produces vasoconstriction, via activation of ETA and ETB receptors on vascular smooth muscle, and vasodilatation via ETB receptors on endothelial cells. Here we have used the...

The contribution of tumour necrosis factor-alpha and endothelin-1 to the increase of coronary resistance in hearts from rats treated with endotoxin.

Hohlfeld, T., Klemm, P., Thiemermann, C., Warner, T. D., Schrör, K., Vane, J. R.

1. Inflammatory disease states predispose to myocardial infarction. Here we have investigated the effects of a systemic inflammatory response syndrome, i.e. lipopolysaccharide (LPS)-induced...

Involvement of endothelin in the pressor response following injection of NMDA to the periaqueductal gray area of rats.

D'Amico, M., Warner, T. D.

Microinjection of N-methyl-D-aspartate (NMDA) (0.068 to 6.8 nmol) into the periaqueductal gray area (PAG) of anaesthetized rats caused dose-dependent increases in blood pressure. Preinjection (10 min...

Endothelin-1 and the periaqueductal gray area of the rat: an autoradiographic and functional pharmacological study.

D'Amico, M., Dashwood, M. R., Warner, T. D.

Endothelin-1 (ET-1) injected centrally induces pressor effects and associated haemodynamic changes. Here we have evaluated the effects on systemic and regional cardiovascular parameters of injection...

Evidence from receptor antagonists of an important role for ETB receptor-mediated vasoconstrictor effects of endothelin-1 in the rat kidney.

Wellings, R. P., Corder, R., Warner, T. D., Cristol, J. P., Thiemermann, C., Vane, J. R.

1. To characterize the receptor subtype(s) mediating the renal vasoconstrictor effects of the endothelin (ET) and sarafotoxin (SX) peptides in the isolated perfused kidney of the rat, we have...

5-HT3 receptors do not mediate vagally-induced relaxation or contraction of the isolated stomach of the guinea-pig.

Desai, K. M., Warner, T. D., Vane, J. R.

1. We have tested whether 5-HT3 receptors mediate vagally-induced relaxation or contraction of the isolated stomach of the guinea-pig. 2. The antagonists of 5-HT3 receptors, ondansetron (1-10 microM)...

Characterization of ETB receptors mediating contractions induced by endothelin-1 or IRL 1620 in guinea-pig isolated airways: effects of BQ-123, FR139317 or PD 145065.

Battistini, B., Warner, T. D., Fournier, A., Vane, J. R.

1. We have characterized the receptors mediating contractions to endothelin-1 (ET-1) or IRL 1620, an ETB receptor selective agonist, in isolated strips of tissue prepared from different parts of the...

Characterization of endothelin (ET) receptors in the isolated gall bladder of the guinea-pig: evidence for an additional ET receptor subtype.

Battistini, B., O'Donnell, L. J., Warner, T. D., Fournier, A., Farthing, M. J., Vane, J. R.

1. We have characterized the receptors mediating contractions induced by endothelin-1 (ET-1), ET-2, ET-3 and the ETB-selective receptor agonists, sarafotoxin 6c (SX6c), IRL 1620, BQ-3020,...

The effect of nitric oxide synthase inhibition on the plasma fibrinolytic system in septic shock in rats.

Korbut, R., Warner, T. D., Gryglewski, R. J., Vane, J. R.

1. We have investigated the effect of pretreatment of rats with nitric oxide (NO) synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME) on the E. coli lipopolysaccharide (LPS)-induced changes...

Reversal of established responses to endothelin-1 in vivo and in vitro by the endothelin receptor antagonists, BQ-123 and PD 145065.

Warner, T. D., Allcock, G. H., Vane, J. R.

1. Endothelin-1 binds almost irreversibly to its receptors and causes prolonged vasoconstrictions. Here we have studied the reversal of established responses to ET-1 in vivo and in vitro by BQ-123,...

Simultaneous perfusion of rat isolated superior mesenteric arterial and venous beds: comparison of their vasoconstrictor and vasodilator responses to agonists.

Warner, T. D.

1. A new isolated perfused preparation is described that allows a direct comparison to be made of the responses of the perfused arterial and retrogradely perfused venous circulations of the rat...

Induction by endotoxin of nitric oxide synthase in the rat mesentery: lack of effect on action of vasoconstrictors.

Mitchell, J. A., Kohlhaas, K. L., Sorrentino, R., Warner, T. D., Murad, F., Vane, J. R.

1. Male Sprague-Dawley or Wistar rats were injected with bacterial lipopolysaccharide (LPS; 5 mg kg-1, i.p.) and killed after 1, 3, 6, 15, and 24 h. The brains, mesenteries, spleens, lungs, livers,...

Characterization of receptors for endothelins in the perfused arterial and venous mesenteric vasculatures of the rat.

D'Orléans-Juste, P., Claing, A., Warner, T. D., Yano, M., Télémaque, S.

1. Endothelin-1 and -3 induced marked arterial and venous constrictions in the perfused mesenteric vasculature of the rat with endothelin-3 being at least 20 times less active than endothelin-1, on...

Use of the endothelin antagonists BQ-123 and PD 142893 to reveal three endothelin receptors mediating smooth muscle contraction and the release of EDRF.

Warner, T. D., Allcock, G. H., Corder, R., Vane, J. R.

1. We have compared the receptors mediating the contractions of rings of rat thoracic aorta or rabbit pulmonary artery and rat stomach strips in response to the endothelin/sarafotoxin (ET/SX) family...

Characterization of endothelin receptors mediating the effects of the endothelin/sarafotoxin peptides on autonomic neurotransmission in the rat vas deferens and guinea-pig ileum.

Warner, T. D., Allcock, G. H., Mickley, E. J., Vane, J. R.

1. To characterize the receptors mediating the effects of the endothelin/sarafotoxin family of peptides on the responses to electrical stimulation of the rat vas deferens (RVD) and guinea-pig ileum...

Modulation of colony stimulating factor release and apoptosis in human colon cancer cells by anticancer drugs

Calatayud, S, Warner, T D, Mitchell, J A

Modulation of the immune response against tumour cells is emerging as a valuable approach for cancer treatment. Some experimental studies have shown that secretion of colony stimulating factors by...