Megabits secure key rate quantum key distribution (2008)
Zhang, Q., Takesue, H., Honjo, T., Wen, K., Hirohata, T., Suyama, M., ...
Quantum cryptography (QC) can provide unconditional secure communication between two authorized parties based on the basic principles of quantum mechanics. However, imperfect practical conditions...
The monolayer formation of Bergmann glial cells is regulated by Notch/RBP-J signaling (2007)
Komine, O., Nagaoka, M., Watase, K., Gutmann, D. H., Tanigaki, K., Honjo, T., ...
The Bergmann glia is a unipolar astrocyte in the cerebellar cortex, displaying a tight association with Purkinje cells. The cell bodies of Bergmann glia are located in a row around Purkinje cell...
Differential phase shift quantum key distribution experiment over 105 km fibre (2005)
Takesue, H., Diamanti, E., Honjo, T., Langrock, C., Fejer, M. M., Inoue, K., ...
Since several papers appeared in 2000, the quantum key distribution (QKD) community has been well aware that photon number splitting (PNS) attack by Eve severely limits the secure key distribution...
Watanabe, N, Ikuta, K, Okazaki, K, Nakase, H, Tabata, Y, Matsuura, M, ...
Shinohara, T., Inoue, K., Ogonuki, N., Kanatsu-Shinohara, M., Miki, H., Nakata, K., ...
BACKGROUND: Fertility protection is an urgent clinical problem for prepubertal male oncology patients who undergo either chemotherapy or radiotherapy. As these patients do not have mature sperm to be...
Nakabayashi, Y., Ikeda, S., Kohno, M., Honjo, T., Tsuchiyama, T.
There are some technical difficulties in manufacturing a large A286 alloy rotor forging, increases in freckling tendency and notch sensitivity, but decrease in tensile and creep rupture strength; the...
Manufacturing and Mechanical Properties of a Large Size Alloy 706 Disk by ESR Process. (1998)
Honjo, T., Matsumoto, O., Morita, K., Hirose, K., Okamura, M.
This paper concerned metals in some important way and contained information about, utilized, or discussed these subjects: basic topics: experimental materials and material types:...
Sauzet, Jean-Pierre, Gras-Masse, Helene, Guillet, Jean-Gerard, Gomard, Elisabeth, Honjo, T
Free unmodified peptides are poor immunogens for cytotoxic T lymphocytes (CTL) in mice, unless they are injected with adjuvant. Although it is generally accepted that CD4+ Th cells are essential...
Mizuta, Ryushin, Taccioli, Guillermo E., Alt, Frederick W., Honjo, T.
Defective expression of the Ku80 gene has been implicated as underlying the V(D)J recombination and DNA double-strand break repair defects in the xrs-6 Chinese hamster ovary cell line. We now show...
Nilsson, Lars, Grant, Patrick, Larsson, Irene, Pettersson, Sven, Sideras, Paschalis, Honjo, T.
It appears that the switch recombination machinery of a B lymphocyte targets preferentially unrearranged heavy chain genes that have been rendered transcriptionally active. Transcriptional activation...
Lars, Nllsson, Paschalis, Sideras, Honjo, T.
Treatment of human splenic B lymphocytes with the mitogen Branhamella catarrhalis (BC) and transforming growth factor-β (TGF-β) induces expression of germline Ig Cα transcripts and class switching...
Kannagi, Mari, Harada, Shinji, Maruyama, Ikuro, Inoko, Hidetoshi, Igarashi, Hisanaga, Kuwashima, Gorou, ...
We established long-term cell lines of cytotoxic T lymphocytes (CTL) specific for human T cell leukemia virus type I (HTLV-I) from peripheral blood lymphocytes (PBL) of a patient with...
Isolation of a T cell specific cDNA clone possibly involved in the T cell activation pathway (1989)
Takihara, Yoshlhiro, Caccia, Nicolette, Yanagi, Yusuke, Mak, Tak W., Honjo, T.
A T cell specific cDNA clone, YT15, has been isolated using the technique of subtractive hybrldlzation. This gene is expressed predominantly in thymocytes and encodes a newly identified...
Preferential usage of JH2 in D-J joinings with DQ52 in murine lymphocytes (1989)
Suzuki, Haruhiko, Abe, Masumi, Nishikawa, Shi-ichi, Nakayama, Eiichi, Shiku, Hiroshi, Honjo, T
Southern blot analysis of genomic DNA of normal mouse thymocytes with a JH4 probe, a probe for JH4 of Igh genes, consistently showed a second band in addition to a germiine band. The same band was...
Ohno, H, Furukawa, T, Fukuhara, S, Zong, S Q, Kamesaki, H, Shows, T B, ...
We have studied a translocation, t(9;14)(p13;q32), in a diffuse large-cell lymphoma cell line, KIS-1, that expresses the Ki-1 (CD30) antigen. Molecular cloning of the immunoglobulin heavy-chain locus...
Sugai, M, Kondo, S, Shimizu, A, Honjo, T
Immunoglobulin class switch recombination enables B lymphocytes to sequentially express antibodies that have identical specificities but that differ in class and effector function. Although several...
Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region.
Kurooka, H, Kuroda, K, Honjo, T
The Notch intracellular region (RAMIC) interacts with a DNA binding protein RBP-J to activate transcription of genes that inhibit cell differentiation. The RAM domain and ankyrin (ANK) repeats of...
RBP-L, a transcription factor related to RBP-Jkappa.
Minoguchi, S, Taniguchi, Y, Kato, H, Okazaki, T, Strobl, L J, Zimber-Strobl, U, ...
RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at...
Occurrence of respiratory syncytial virus subgroup A and B strains in Japan, 1980 to 1987.
Tsutsumi, H, Onuma, M, Suga, K, Honjo, T, Chiba, Y, Chiba, S, ...
The subgroup characteristics of 71 strains of respiratory syncytial virus (RSV) isolated in Sapporo, Japan, during 5 epidemic years from 1980 to 1987 were determined by the use of 17 monoclonal...
Tsutsumi, H, Honjo, T, Nagai, K, Chiba, Y, Chiba, S, Tsugawa, S
Immunoglobulin A (IgA) antibody response to respiratory syncytial virus (RSV) structural proteins in colostrum and milk was investigated by a radioimmunoprecipitation assay. By using...
Kinashi, T, Inaba, K, Tsubata, T, Tashiro, K, Palacios, R, Honjo, T
Precursors to B-cell lines with immunoglobulin genes in the germ-line context have been shown to be capable of generating mature B cells in vivo. We report here that an interleukin 3-dependent...
Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines.
Mita, S, Tominaga, A, Hitoshi, Y, Sakamoto, K, Honjo, T, Akagi, M, ...
Interleukin 5 (IL-5) is a glycosylated polypeptide that acts as a key factor for B-cell growth and differentiation. We demonstrated previously that there are two classes (high and low affinity) of...
Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse.
Shimizu, A, Nussenzweig, M C, Mizuta, T R, Leder, P, Honjo, T
We have studied immunoglobulin double-isotype expression in a transgenic mouse (TG.SA) in which expression of the endogenous immunoglobulin heavy chain locus is almost completely excluded by a...
Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins.
Ikuta, K, Takami, M, Kim, C W, Honjo, T, Miyoshi, T, Tagaya, Y, ...
We have purified the human lymphocyte Fc receptor specific for IgE (Fc epsilon receptor) and its soluble form by using the anti-Fc epsilon receptor monoclonal antibody H107. Using an oligonucleotide...
Sakoyama, Y, Hong, K J, Byun, S M, Hisajima, H, Ueda, S, Yaoita, Y, ...
To determine the phylogenetic relationships among hominoids and the dates of their divergence, the complete nucleotide sequences of the constant region of the immunoglobulin epsilon-chain (C epsilon...
Interleukin 5, a T-cell-derived B-cell differentiation factor also induces cytotoxic T lymphocytes.
Takatsu, K, Kikuchi, Y, Takahashi, T, Honjo, T, Matsumoto, M, Harada, N, ...
We describe an interleukin, termed interleukin 5, that is the recombinant product previously referred to as T-cell-replacing factor (TRF), B-cell growth factor II (BCGF II), or killer-helper factor...
Harada, N, Takahashi, T, Matsumoto, M, Kinashi, T, Ohara, J, Kikuchi, Y, ...
T-cell-replacing factor (TRF) is a T-cell-derived factor required for terminal differentiation of activated B cells to immunoglobulin-secreting cells. Previous studies have shown that a murine T-cell...
Recognition sequence of a highly conserved DNA binding protein RBP-J kappa.
Tun, T, Hamaguchi, Y, Matsunami, N, Furukawa, T, Honjo, T, Kawaichi, M
DNA binding specificity of the RBP-J kappa protein was extensively examined. The mouse RBP-J kappa protein was originally isolated as a nuclear protein binding to the J kappa type V(D)J recombination...
Mizuta, T R, Fukita, Y, Miyoshi, T, Shimizu, A, Honjo, T
We have isolated the cDNA encoding a binding protein to the sequence motif of the immunoglobulin S mu region by the southwestern method. The binding protein designated S mu bp-2 specifically binds to...
Chung, C N, Hamaguchi, Y, Honjo, T, Kawaichi, M
To map regions important for DNA binding of the mouse homologue of Suppressor of Hairless or RBP-J kappa protein, mutated mouse RBP-J kappa cDNAs were made by insertion of oligonucleotide linkers or...
Matsunami, N, Siomi, H, Hatanaka, M, Yaoita, Y, Honjo, T
The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their...
Ikuta, K, Ogura, T, Shimizu, A, Honjo, T
We have analysed an inactive allele of the beta-chain gene of the T-cell receptor in a human T-cell line HPB-ALL. Comparison with germline sequences showed that HPB-ALL has a joining (J)-diversity...
Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster.
Nishida, Y, Kataoka, T, Ishida, N, Nakai, S, Kishimoto, T, Böttcher, I, ...
Mouse immunoglobulin epsilon chain gene was cloned from DNA of a hybridoma producing anti-dinitrophenyl IgE, which was constructed by fusing a spleen cell of a BALB/c mouse with a variant clone of...
Obata, M, Kataoka, T, Nakai, S, Yamagishi, H, Takahashi, N, Yamawaki-Kataoka, Y, ...
An expressed gene for gamma 1 chain of MC 101 myeloma was cloned from a phage library containing partial EcoRI digests of MC 101 DNA. The cloned DNA was analyzed by restriction enzyme cleavage,...
Kataoka, T, Kondo, S, Nishi, M, Kodaira, M, Honjo, T
An endonuclease activity which cleaves close to the recombination sites of the immunoglobulin JK segments was found in extracts of chicken bursa of Fabricius and characterized after partial...
Immunoglobulin switch region-like sequences in Drosophila melanogaster.
Sakoyama, Y, Yaoita, Y, Honjo, T
We found immunoglobulin switch (S) region-like sequences in DNAs of wide variety of organisms including sea urchin, yeast and Drosophila that do not produce immunoglobulins. DNA fragments carrying...
Molecular cloning and structure of the human interleukin 2 receptor gene.
Ishida, N, Kanamori, H, Noma, T, Nikaido, T, Sabe, H, Suzuki, N, ...
We have cloned the IL-2 receptor gene from human genomic DNA libraries using IL-2 receptor cDNA as probe. The genomic DNA segments that hybridized with cDNA were subcloned in M13 phages and their...
Kawakami, T, Takahashi, N, Honjo, T
We have determined the complete nucleotides sequence (2168 bases) of the immunoglobulin mu gene cloned from newborn mouse DNA. The cloned 13kb fragment contained the entire constant region gene...
Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain cDNA.
Seno, M, Kurokawa, T, Ono, Y, Onda, H, Sasada, R, Igarashi, K, ...
DNA complementary to mRNA of human immunoglobulin E heavy chain (epsilon chain) isolated and purified from U266 cells has been synthesized and inserted into the PstI site of pBR322 by G-C tailing....
Expression of human immunoglobulin E epsilon chain cDNA in E. coli.
Kurokawa, T, Seno, M, Sasada, R, Ono, Y, Onda, H, Igarashi, K, ...
Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C...
Comparison of nucleotide sequences of a macronuclear DNA and its micronuclear counterpart of a hypotrichous ciliate, Stylonychia pustulata, demonstrates that common terminal repeats (C4A4) of the...
The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
Yaoita, Y, Matsunami, N, Choi, C Y, Sugiyama, H, Kishimoto, T, Honjo, T
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of...
Yamawaki-Kataoka, Y, Miyata, T, Honjo, T
We have determined the complete nucleotide sequence (1990 base pairs) of mouse immunoglobulin gamma 2a gene, and compared it with the sequences of other gamma subclass genes so far sequenced, i.e....
Cloning of human immunoglobulin mu gene and comparison with mouse mu gene.
Takahashi, N, Nakai, S, Honjo, T
We have cloned a 12 kb DNA segment containing human mu gene and its flanking sequence from human fetal liver DNA library using mouse mu gene as a probe. Partial nucleotide sequence determination...
Nakatani, T, Horigome, K, Nomura, N, Kondo, T, Ohtsuka, H, Noguchi, H, ...
Mapping and partial sequencing of the productive K chain genomic DNA of FK-001 demonstrated a 1.8-kb deletion including the JK2, JK3, JK4, and JK5 segments. This deletion occurred between the...
Hamaguchi, Y, Matsunami, N, Yamamoto, Y, Honjo, T
A protein that binds to the recombination signal sequence (RS) of the immunoglobulin J kappa segment was purified almost to homogeneity from the nuclear extract of a murine pre-B cell line 38B9. A...
Shimuzu, A, Kondo, S, Takeda, S, Yodoi, J, Ishida, N, Sabe, H, ...
We have cloned cDNA encoding the mouse interleukin-2 (IL-2) receptor from a murine T cell line, CTLL using human IL-2 receptor cDNA as probe. COS 7 cells transfected with the cDNA expressed the...
Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin gamma chains.
Yamawaki-Kataoka, Y, Nakai, S, Miyata, T, Honjo, T
The nucleotide sequences of the exons encoding membrane-bound IgG1, IgG2a, and IgG2b of mouse were determined and compared with the sequence of membrane-bound IgM. The sequences indicate that...
Cloning of human immunoglobulin epsilon chain genes: evidence for multiple C epsilon genes.
Nishida, Y, Miki, T, Hisajima, H, Honjo, T
An active human epsilon chain gene was cloned from a phage library containing partial EcoRI digests of IgE-producing myeloma DNA, using the human JH (joining) gene fragment as a probe. The epsilon...
A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.
Nakanishi, K, Sugimura, K, Yaoita, Y, Maeda, K, Kashiwamura, S, Honjo, T, ...
The T suppressive factor (TsF) released from a T15-idiotype-positive phosphocholine (PCho)-specific T hybridoma, F18-3-4, which was formed by fusion between BALB/c T cells and BW5147 thymoma, was...
Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
Kataoka, T, Kawakami, T, Takahashi, N, Honjo, T
Cloning and nucleotide sequence determination suggest that the rearranged gamma 1-chain gene in a gamma 1-chain-producing myeloma a-pears to be formed by the recombination between the 5' flanking...
Miyata, T, Yasunaga, T, Yamawaki-Kataoka, Y, Obata, M, Honjo, T
The nucleotide sequences of the constant portions of mouse immunoglobulin gamma 1 and gamma 2b genes were compared. A remarkable homology was found in a long (about 500 nucleotides) continuous...
The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.
Dou, S, Zeng, X, Cortes, P, Erdjument-Bromage, H, Tempst, P, Honjo, T, ...
We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was...
Otsu, M, Katamine, S, Uno, M, Yamaki, M, Ono, Y, Klein, G, ...
An in vitro culture of FLEB14 cells, an Epstein-Barr virus-transformed B cell precursor containing the germ line immunoglobulin genes, gave rise to a uniclonally expanded variant, FLEB14 delta 3,...
Kondo, S, Shimizu, A, Saito, Y, Kinoshita, M, Honjo, T
Two affinity species of the interleukin 2 (IL-2) receptor are different states of a single receptor molecule. We assumed that a binary complex between the IL-2 receptor and another...
Nishi, M, Kataoka, T, Honjo, T
The V kappa-KpnI family, which constitutes approximately equal to 36% of mouse kappa chain variable region gene (V kappa) segments, conserves the Kpn I site (G-G-T-A-C-C) at the position...
Low frequency of somatic mutation in beta-chain variable region genes of human T-cell receptors.
Ikuta, K, Ogura, T, Shimizu, A, Honjo, T
We have cloned three pairs of rearranged and germ-line variable region (V beta) genes of the beta chain of the human T-cell receptor from the cell lines ATL2, ATL12, and MT-1 of patients with adult...
Takahashi, N, Noma, T, Honjo, T
We have cloned two rearranged heavy chain variable region (VH) genes from the IgG-producing human cell line CESS. The VH gene, which is linked to the mu chain constant region (C mu) gene, has two...
Nonaka, M, Takahashi, M, Natsuume-Sakai, S, Nonaka, M, Tanaka, S, Shimizu, A, ...
cDNA clones specific for the fourth component of mouse complement (C4) and its hormonally regulated isotype, sex-linked protein (Slp), were isolated using as a probe a 20-mer synthetic...
Polymorphisms of a human variable heavy chain gene show linkage with constant heavy chain genes.
Johnson, M J, Natali, A M, Cann, H M, Honjo, T, Cavalli-Sforza, L L
In this study, restriction fragment length polymorphisms (RFLPs) were identified with an immunoglobulin variable heavy chain region (Ig VH) probe and the inheritance of the polymorphisms was analyzed...
Organization of immunoglobulin heavy chain genes and allelic deletion model.
We have assessed the number of times the gene sequence encoding constant regions of mouse immunoglobulin heavy chains gamma1, gamma2a, and gamma3 are represented in the mouse genome by hybridization...
Migone, N, Feder, J, Cann, H, Van West, B, Hwang, J, Takahashi, N, ...
DNA probes containing the switch region (S) associated with the human immunoglobulin heavy chain mu gene were used to investigate polymorphisms in the germ-line human DNA. Six polymorphisms, detected...
Escherichia coli extract-catalyzed recombination in switch regions of mouse immunoglobulin genes.
Kataoka, T, Takeda, S, Honjo, T
We have shown that Escherichia coli extracts catalyze recombination between mouse immunoglobulin mu and alpha genes inserted separately in lambda phage vectors carrying different genetic markers....
Hisajima, H, Nishida, Y, Nakai, S, Takahashi, N, Ueda, S, Honjo, T
Cloning of the overlapping DNA fragments together with Southern hybridization experiments showed the organization of the human C epsilon and C alpha gene cluster as 5'-C epsilon 2-14 kilobases-C...
Zimber-Strobl, U, Strobl, L J, Meitinger, C, Hinrichs, R, Sakai, T, Furukawa, T, ...
Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays a crucial role in B cell immortalization by Epstein-Barr virus (EBV), most probably by its ability to transactivate several cellular and viral...
A truncated immunoglobulin epsilon pseudogene is found in gorilla and man but not in chimpanzee.
Ueda, S, Takenaka, O, Honjo, T
Molecular genetic analyses of the young pseudogenes of the immunoglobulin C epsilon genes were carried out to obtain qualitative evidence for the phylogenetic branching pattern of hominoid primates....
Kataoka, T, Yamawaki-Kataoka, Y, Yamagishi, H, Honjo, T
DNA from newborn mice was digested with restriction endonuclease EcoRI, and a 6.6-kilobase fragment encoding immunoglobulin gamma 2b chain mRNA derived from MPC 11 myeloma was enriched about 100-fold...
Honjo, T., Packman, S., Swan, D., Nau, M., Leder, P.
Hybridization kinetic analyses with synthetic DNA indicate that there are only two to three copies of the κ constant region gene per haploid genome. This result lends weight to the argument that the...
Nosaka, T, Ohno, H, Doi, S, Fukuhara, S, Miwa, H, Kita, K, ...
Acute biphenotypic leukemia composed of lymphoblasts and myeloblasts developed in a patient with T lymphoblastic lymphoma (T-LBL) who had an anterior mediastinal mass. A novel myeloid cell line,...
Era, T, Ogawa, M, Nishikawa, S, Okamoto, M, Honjo, T, Akagi, K, ...
During B cell differentiation, at least three stages can be defined in terms of their growth signal requirement by using two different growth signals, which are recombinant interleukin 7 (IL-7) and a...
Shin, E K, Matsuda, F, Nagaoka, H, Fukita, Y, Imai, T, Yokoyama, K, ...
We have constructed the physical map of the 3' region of the human immunoglobulin heavy chain variable region (VH) genes. DNA segments extending to 200 kb upstream of the JH segment were isolated in...
Dispersed localization of D segments in the human immunoglobulin heavy-chain locus.
Matsuda, F, Lee, K H, Nakai, S, Sato, T, Kodaira, M, Zong, S Q, ...
We have studied the organization of the human immunoglobulin heavy-chain genes by pulse field gel electrophoresis as well as by isolation of cosmid clones. The total length of the heavy-chain...
Aoki, T, Tashiro, K, Miyatake, S, Kinashi, T, Nakano, T, Oda, Y, ...
We have examined the immunoregulatory effect of local and continuous secretion of interleukin 7 (IL-7) from murine glioma cells (203-glioma) engineered by murine IL-7 gene transfection. Secretion of...
HTLV-1 p27rex stabilizes human interleukin-2 receptor alpha chain mRNA.
Kanamori, H, Suzuki, N, Siomi, H, Nosaka, T, Sato, A, Sabe, H, ...
Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces...
Matsuda, F, Shin, E K, Hirabayashi, Y, Nagaoka, H, Yoshida, M C, Zong, S Q, ...
We have studied the organization of variable region (V) genes of the human immunoglobulin heavy chain (H) by cosmid cloning. We isolated two independent immunoglobulin D5 clusters (D5-a and D5-b)...
Ishida, N, Ueda, S, Hayashida, H, Miyata, T, Honjo, T
We have determined the nucleotide sequence of the immunoglobulin epsilon gene cloned from newborn mouse DNA. The epsilon gene sequence allows prediction of the amino acid sequence of the constant...
Ueda, S, Nakai, S, Nishida, Y, Hisajima, H, Honjo, T
There are at least three immunoglobulin epsilon genes (C epsilon 1, C epsilon 2, and C epsilon 3) in the human genome. The nucleotide sequences of the expressed epsilon gene (C epsilon 1) and one (C...
Ishida, Y, Agata, Y, Shibahara, K, Honjo, T
The classical type of programmed cell death is characterized by its dependence on de novo RNA and protein synthesis and morphological features of apoptosis. We confirmed that stimulated 2B4.11 (a...
Kobayashi, N, Konishi, H, Sabe, H, Shigesada, K, Noma, T, Honjo, T, ...
We studied the genomic structure of human T-cell leukemia virus (HTLV) in the HTLV producer cell line MT-2. Southern blotting revealed that at least eight HTLV proviruses were integrated in the...
Concerted evolution of the mouse immunoglobulin gamma chain genes.
Hayashida, H, Miyata, T, Yamawaki-Kataoka, Y, Honjo, T, Wels, J, Blattner, F
The nucleotide sequences of the immunoglobulin heavy-chain constant region genes of mouse, C gamma 3, C gamma 1, C gamma 2b and C gamma 2a, together with that of a human equivalent C gamma 4 were...
Tohyama, K, Lee, K H, Tashiro, K, Kinashi, T, Honjo, T
An interleukin-5 (IL-5)-dependent subclone, K-5, was established from an IL-3-dependent murine hemopoietic progenitor cell line by co-culturing with bone marrow stroma cells. K-5 cells were induced...
Ohno, H, Furukawa, T, Fukuhara, S, Zong, S Q, Kamesaki, H, Shows, T B, ...
We have studied a translocation, t(9;14)(p13;q32), in a diffuse large-cell lymphoma cell line, KIS-1, that expresses the Ki-1 (CD30) antigen. Molecular cloning of the immunoglobulin heavy-chain locus...
Sugai, M, Kondo, S, Shimizu, A, Honjo, T
Immunoglobulin class switch recombination enables B lymphocytes to sequentially express antibodies that have identical specificities but that differ in class and effector function. Although several...
Roles of the ankyrin repeats and C-terminal region of the mouse notch1 intracellular region.
Kurooka, H, Kuroda, K, Honjo, T
The Notch intracellular region (RAMIC) interacts with a DNA binding protein RBP-J to activate transcription of genes that inhibit cell differentiation. The RAM domain and ankyrin (ANK) repeats of...
RBP-L, a transcription factor related to RBP-Jkappa.
Minoguchi, S, Taniguchi, Y, Kato, H, Okazaki, T, Strobl, L J, Zimber-Strobl, U, ...
RBP-Jkappa is a sequence-specific DNA binding protein which plays a central role in signalling downstream of the Notch receptor by physically interacting with its intracellular region. Although at...
Occurrence of respiratory syncytial virus subgroup A and B strains in Japan, 1980 to 1987.
Tsutsumi, H, Onuma, M, Suga, K, Honjo, T, Chiba, Y, Chiba, S, ...
The subgroup characteristics of 71 strains of respiratory syncytial virus (RSV) isolated in Sapporo, Japan, during 5 epidemic years from 1980 to 1987 were determined by the use of 17 monoclonal...
Tsutsumi, H, Honjo, T, Nagai, K, Chiba, Y, Chiba, S, Tsugawa, S
Immunoglobulin A (IgA) antibody response to respiratory syncytial virus (RSV) structural proteins in colostrum and milk was investigated by a radioimmunoprecipitation assay. By using...
Kinashi, T, Inaba, K, Tsubata, T, Tashiro, K, Palacios, R, Honjo, T
Precursors to B-cell lines with immunoglobulin genes in the germ-line context have been shown to be capable of generating mature B cells in vivo. We report here that an interleukin 3-dependent...
Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines.
Mita, S, Tominaga, A, Hitoshi, Y, Sakamoto, K, Honjo, T, Akagi, M, ...
Interleukin 5 (IL-5) is a glycosylated polypeptide that acts as a key factor for B-cell growth and differentiation. We demonstrated previously that there are two classes (high and low affinity) of...
Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse.
Shimizu, A, Nussenzweig, M C, Mizuta, T R, Leder, P, Honjo, T
We have studied immunoglobulin double-isotype expression in a transgenic mouse (TG.SA) in which expression of the endogenous immunoglobulin heavy chain locus is almost completely excluded by a...
Human lymphocyte Fc receptor for IgE: sequence homology of its cloned cDNA with animal lectins.
Ikuta, K, Takami, M, Kim, C W, Honjo, T, Miyoshi, T, Tagaya, Y, ...
We have purified the human lymphocyte Fc receptor specific for IgE (Fc epsilon receptor) and its soluble form by using the anti-Fc epsilon receptor monoclonal antibody H107. Using an oligonucleotide...
Sakoyama, Y, Hong, K J, Byun, S M, Hisajima, H, Ueda, S, Yaoita, Y, ...
To determine the phylogenetic relationships among hominoids and the dates of their divergence, the complete nucleotide sequences of the constant region of the immunoglobulin epsilon-chain (C epsilon...
Interleukin 5, a T-cell-derived B-cell differentiation factor also induces cytotoxic T lymphocytes.
Takatsu, K, Kikuchi, Y, Takahashi, T, Honjo, T, Matsumoto, M, Harada, N, ...
We describe an interleukin, termed interleukin 5, that is the recombinant product previously referred to as T-cell-replacing factor (TRF), B-cell growth factor II (BCGF II), or killer-helper factor...
Harada, N, Takahashi, T, Matsumoto, M, Kinashi, T, Ohara, J, Kikuchi, Y, ...
T-cell-replacing factor (TRF) is a T-cell-derived factor required for terminal differentiation of activated B cells to immunoglobulin-secreting cells. Previous studies have shown that a murine T-cell...
Recognition sequence of a highly conserved DNA binding protein RBP-J kappa.
Tun, T, Hamaguchi, Y, Matsunami, N, Furukawa, T, Honjo, T, Kawaichi, M
DNA binding specificity of the RBP-J kappa protein was extensively examined. The mouse RBP-J kappa protein was originally isolated as a nuclear protein binding to the J kappa type V(D)J recombination...
Mizuta, T R, Fukita, Y, Miyoshi, T, Shimizu, A, Honjo, T
We have isolated the cDNA encoding a binding protein to the sequence motif of the immunoglobulin S mu region by the southwestern method. The binding protein designated S mu bp-2 specifically binds to...
Chung, C N, Hamaguchi, Y, Honjo, T, Kawaichi, M
To map regions important for DNA binding of the mouse homologue of Suppressor of Hairless or RBP-J kappa protein, mutated mouse RBP-J kappa cDNAs were made by insertion of oligonucleotide linkers or...
Matsunami, N, Siomi, H, Hatanaka, M, Yaoita, Y, Honjo, T
The promoters of the adenovirus 2 major late gene, the mouse beta-globin gene, the mouse immunoglobulin VH gene and the LTR of the human T-lymphotropic retrovirus type I were tested for their...
Ikuta, K, Ogura, T, Shimizu, A, Honjo, T
We have analysed an inactive allele of the beta-chain gene of the T-cell receptor in a human T-cell line HPB-ALL. Comparison with germline sequences showed that HPB-ALL has a joining (J)-diversity...
Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster.
Nishida, Y, Kataoka, T, Ishida, N, Nakai, S, Kishimoto, T, Böttcher, I, ...
Mouse immunoglobulin epsilon chain gene was cloned from DNA of a hybridoma producing anti-dinitrophenyl IgE, which was constructed by fusing a spleen cell of a BALB/c mouse with a variant clone of...
Obata, M, Kataoka, T, Nakai, S, Yamagishi, H, Takahashi, N, Yamawaki-Kataoka, Y, ...
An expressed gene for gamma 1 chain of MC 101 myeloma was cloned from a phage library containing partial EcoRI digests of MC 101 DNA. The cloned DNA was analyzed by restriction enzyme cleavage,...
Kataoka, T, Kondo, S, Nishi, M, Kodaira, M, Honjo, T
An endonuclease activity which cleaves close to the recombination sites of the immunoglobulin JK segments was found in extracts of chicken bursa of Fabricius and characterized after partial...
Immunoglobulin switch region-like sequences in Drosophila melanogaster.
Sakoyama, Y, Yaoita, Y, Honjo, T
We found immunoglobulin switch (S) region-like sequences in DNAs of wide variety of organisms including sea urchin, yeast and Drosophila that do not produce immunoglobulins. DNA fragments carrying...
Molecular cloning and structure of the human interleukin 2 receptor gene.
Ishida, N, Kanamori, H, Noma, T, Nikaido, T, Sabe, H, Suzuki, N, ...
We have cloned the IL-2 receptor gene from human genomic DNA libraries using IL-2 receptor cDNA as probe. The genomic DNA segments that hybridized with cDNA were subcloned in M13 phages and their...
Kawakami, T, Takahashi, N, Honjo, T
We have determined the complete nucleotides sequence (2168 bases) of the immunoglobulin mu gene cloned from newborn mouse DNA. The cloned 13kb fragment contained the entire constant region gene...
Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain cDNA.
Seno, M, Kurokawa, T, Ono, Y, Onda, H, Sasada, R, Igarashi, K, ...
DNA complementary to mRNA of human immunoglobulin E heavy chain (epsilon chain) isolated and purified from U266 cells has been synthesized and inserted into the PstI site of pBR322 by G-C tailing....
Expression of human immunoglobulin E epsilon chain cDNA in E. coli.
Kurokawa, T, Seno, M, Sasada, R, Ono, Y, Onda, H, Igarashi, K, ...
Using the cDNA of human epsilon chain, three expression plasmids that code directly the constant portion of the epsilon chain (C epsilon 1-C epsilon 4, C epsilon 2-C epsilon 4 and C epsilon 3-C...
Comparison of nucleotide sequences of a macronuclear DNA and its micronuclear counterpart of a hypotrichous ciliate, Stylonychia pustulata, demonstrates that common terminal repeats (C4A4) of the...
The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
Yaoita, Y, Matsunami, N, Choi, C Y, Sugiyama, H, Kishimoto, T, Honjo, T
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of...
Yamawaki-Kataoka, Y, Miyata, T, Honjo, T
We have determined the complete nucleotide sequence (1990 base pairs) of mouse immunoglobulin gamma 2a gene, and compared it with the sequences of other gamma subclass genes so far sequenced, i.e....
Cloning of human immunoglobulin mu gene and comparison with mouse mu gene.
Takahashi, N, Nakai, S, Honjo, T
We have cloned a 12 kb DNA segment containing human mu gene and its flanking sequence from human fetal liver DNA library using mouse mu gene as a probe. Partial nucleotide sequence determination...
Nakatani, T, Horigome, K, Nomura, N, Kondo, T, Ohtsuka, H, Noguchi, H, ...
Mapping and partial sequencing of the productive K chain genomic DNA of FK-001 demonstrated a 1.8-kb deletion including the JK2, JK3, JK4, and JK5 segments. This deletion occurred between the...
Hamaguchi, Y, Matsunami, N, Yamamoto, Y, Honjo, T
A protein that binds to the recombination signal sequence (RS) of the immunoglobulin J kappa segment was purified almost to homogeneity from the nuclear extract of a murine pre-B cell line 38B9. A...
Shimuzu, A, Kondo, S, Takeda, S, Yodoi, J, Ishida, N, Sabe, H, ...
We have cloned cDNA encoding the mouse interleukin-2 (IL-2) receptor from a murine T cell line, CTLL using human IL-2 receptor cDNA as probe. COS 7 cells transfected with the cDNA expressed the...
Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin gamma chains.
Yamawaki-Kataoka, Y, Nakai, S, Miyata, T, Honjo, T
The nucleotide sequences of the exons encoding membrane-bound IgG1, IgG2a, and IgG2b of mouse were determined and compared with the sequence of membrane-bound IgM. The sequences indicate that...
Cloning of human immunoglobulin epsilon chain genes: evidence for multiple C epsilon genes.
Nishida, Y, Miki, T, Hisajima, H, Honjo, T
An active human epsilon chain gene was cloned from a phage library containing partial EcoRI digests of IgE-producing myeloma DNA, using the human JH (joining) gene fragment as a probe. The epsilon...
A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.
Nakanishi, K, Sugimura, K, Yaoita, Y, Maeda, K, Kashiwamura, S, Honjo, T, ...
The T suppressive factor (TsF) released from a T15-idiotype-positive phosphocholine (PCho)-specific T hybridoma, F18-3-4, which was formed by fusion between BALB/c T cells and BW5147 thymoma, was...
Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.
Kataoka, T, Kawakami, T, Takahashi, N, Honjo, T
Cloning and nucleotide sequence determination suggest that the rearranged gamma 1-chain gene in a gamma 1-chain-producing myeloma a-pears to be formed by the recombination between the 5' flanking...
Miyata, T, Yasunaga, T, Yamawaki-Kataoka, Y, Obata, M, Honjo, T
The nucleotide sequences of the constant portions of mouse immunoglobulin gamma 1 and gamma 2b genes were compared. A remarkable homology was found in a long (about 500 nucleotides) continuous...
The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.
Dou, S, Zeng, X, Cortes, P, Erdjument-Bromage, H, Tempst, P, Honjo, T, ...
We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was...
Otsu, M, Katamine, S, Uno, M, Yamaki, M, Ono, Y, Klein, G, ...
An in vitro culture of FLEB14 cells, an Epstein-Barr virus-transformed B cell precursor containing the germ line immunoglobulin genes, gave rise to a uniclonally expanded variant, FLEB14 delta 3,...
Kondo, S, Shimizu, A, Saito, Y, Kinoshita, M, Honjo, T
Two affinity species of the interleukin 2 (IL-2) receptor are different states of a single receptor molecule. We assumed that a binary complex between the IL-2 receptor and another...
Nishi, M, Kataoka, T, Honjo, T
The V kappa-KpnI family, which constitutes approximately equal to 36% of mouse kappa chain variable region gene (V kappa) segments, conserves the Kpn I site (G-G-T-A-C-C) at the position...
Low frequency of somatic mutation in beta-chain variable region genes of human T-cell receptors.
Ikuta, K, Ogura, T, Shimizu, A, Honjo, T
We have cloned three pairs of rearranged and germ-line variable region (V beta) genes of the beta chain of the human T-cell receptor from the cell lines ATL2, ATL12, and MT-1 of patients with adult...
Takahashi, N, Noma, T, Honjo, T
We have cloned two rearranged heavy chain variable region (VH) genes from the IgG-producing human cell line CESS. The VH gene, which is linked to the mu chain constant region (C mu) gene, has two...
Nonaka, M, Takahashi, M, Natsuume-Sakai, S, Nonaka, M, Tanaka, S, Shimizu, A, ...
cDNA clones specific for the fourth component of mouse complement (C4) and its hormonally regulated isotype, sex-linked protein (Slp), were isolated using as a probe a 20-mer synthetic...
Polymorphisms of a human variable heavy chain gene show linkage with constant heavy chain genes.
Johnson, M J, Natali, A M, Cann, H M, Honjo, T, Cavalli-Sforza, L L
In this study, restriction fragment length polymorphisms (RFLPs) were identified with an immunoglobulin variable heavy chain region (Ig VH) probe and the inheritance of the polymorphisms was analyzed...
Organization of immunoglobulin heavy chain genes and allelic deletion model.
We have assessed the number of times the gene sequence encoding constant regions of mouse immunoglobulin heavy chains gamma1, gamma2a, and gamma3 are represented in the mouse genome by hybridization...
Migone, N, Feder, J, Cann, H, Van West, B, Hwang, J, Takahashi, N, ...
DNA probes containing the switch region (S) associated with the human immunoglobulin heavy chain mu gene were used to investigate polymorphisms in the germ-line human DNA. Six polymorphisms, detected...
Escherichia coli extract-catalyzed recombination in switch regions of mouse immunoglobulin genes.
Kataoka, T, Takeda, S, Honjo, T
We have shown that Escherichia coli extracts catalyze recombination between mouse immunoglobulin mu and alpha genes inserted separately in lambda phage vectors carrying different genetic markers....
Hisajima, H, Nishida, Y, Nakai, S, Takahashi, N, Ueda, S, Honjo, T
Cloning of the overlapping DNA fragments together with Southern hybridization experiments showed the organization of the human C epsilon and C alpha gene cluster as 5'-C epsilon 2-14 kilobases-C...
Zimber-Strobl, U, Strobl, L J, Meitinger, C, Hinrichs, R, Sakai, T, Furukawa, T, ...
Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays a crucial role in B cell immortalization by Epstein-Barr virus (EBV), most probably by its ability to transactivate several cellular and viral...
A truncated immunoglobulin epsilon pseudogene is found in gorilla and man but not in chimpanzee.
Ueda, S, Takenaka, O, Honjo, T
Molecular genetic analyses of the young pseudogenes of the immunoglobulin C epsilon genes were carried out to obtain qualitative evidence for the phylogenetic branching pattern of hominoid primates....
Kataoka, T, Yamawaki-Kataoka, Y, Yamagishi, H, Honjo, T
DNA from newborn mice was digested with restriction endonuclease EcoRI, and a 6.6-kilobase fragment encoding immunoglobulin gamma 2b chain mRNA derived from MPC 11 myeloma was enriched about 100-fold...
Honjo, T., Packman, S., Swan, D., Nau, M., Leder, P.
Hybridization kinetic analyses with synthetic DNA indicate that there are only two to three copies of the κ constant region gene per haploid genome. This result lends weight to the argument that the...
Nosaka, T, Ohno, H, Doi, S, Fukuhara, S, Miwa, H, Kita, K, ...
Acute biphenotypic leukemia composed of lymphoblasts and myeloblasts developed in a patient with T lymphoblastic lymphoma (T-LBL) who had an anterior mediastinal mass. A novel myeloid cell line,...
Era, T, Ogawa, M, Nishikawa, S, Okamoto, M, Honjo, T, Akagi, K, ...
During B cell differentiation, at least three stages can be defined in terms of their growth signal requirement by using two different growth signals, which are recombinant interleukin 7 (IL-7) and a...
Shin, E K, Matsuda, F, Nagaoka, H, Fukita, Y, Imai, T, Yokoyama, K, ...
We have constructed the physical map of the 3' region of the human immunoglobulin heavy chain variable region (VH) genes. DNA segments extending to 200 kb upstream of the JH segment were isolated in...
Dispersed localization of D segments in the human immunoglobulin heavy-chain locus.
Matsuda, F, Lee, K H, Nakai, S, Sato, T, Kodaira, M, Zong, S Q, ...
We have studied the organization of the human immunoglobulin heavy-chain genes by pulse field gel electrophoresis as well as by isolation of cosmid clones. The total length of the heavy-chain...
Aoki, T, Tashiro, K, Miyatake, S, Kinashi, T, Nakano, T, Oda, Y, ...
We have examined the immunoregulatory effect of local and continuous secretion of interleukin 7 (IL-7) from murine glioma cells (203-glioma) engineered by murine IL-7 gene transfection. Secretion of...
Tohyama, K, Lee, K H, Tashiro, K, Kinashi, T, Honjo, T
An interleukin-5 (IL-5)-dependent subclone, K-5, was established from an IL-3-dependent murine hemopoietic progenitor cell line by co-culturing with bone marrow stroma cells. K-5 cells were induced...
HTLV-1 p27rex stabilizes human interleukin-2 receptor alpha chain mRNA.
Kanamori, H, Suzuki, N, Siomi, H, Nosaka, T, Sato, A, Sabe, H, ...
Expression of the pX gene products (p40tax, p27rex and p21X-III) of human T cell leukemia virus type 1 (HTLV-1), which is known to be a causative agent of adult T cell lymphoma/leukemia, induces...
Matsuda, F, Shin, E K, Hirabayashi, Y, Nagaoka, H, Yoshida, M C, Zong, S Q, ...
We have studied the organization of variable region (V) genes of the human immunoglobulin heavy chain (H) by cosmid cloning. We isolated two independent immunoglobulin D5 clusters (D5-a and D5-b)...
Ishida, N, Ueda, S, Hayashida, H, Miyata, T, Honjo, T
We have determined the nucleotide sequence of the immunoglobulin epsilon gene cloned from newborn mouse DNA. The epsilon gene sequence allows prediction of the amino acid sequence of the constant...
Ueda, S, Nakai, S, Nishida, Y, Hisajima, H, Honjo, T
There are at least three immunoglobulin epsilon genes (C epsilon 1, C epsilon 2, and C epsilon 3) in the human genome. The nucleotide sequences of the expressed epsilon gene (C epsilon 1) and one (C...
Ishida, Y, Agata, Y, Shibahara, K, Honjo, T
The classical type of programmed cell death is characterized by its dependence on de novo RNA and protein synthesis and morphological features of apoptosis. We confirmed that stimulated 2B4.11 (a...
Kobayashi, N, Konishi, H, Sabe, H, Shigesada, K, Noma, T, Honjo, T, ...
We studied the genomic structure of human T-cell leukemia virus (HTLV) in the HTLV producer cell line MT-2. Southern blotting revealed that at least eight HTLV proviruses were integrated in the...
Concerted evolution of the mouse immunoglobulin gamma chain genes.
Hayashida, H, Miyata, T, Yamawaki-Kataoka, Y, Honjo, T, Wels, J, Blattner, F
The nucleotide sequences of the immunoglobulin heavy-chain constant region genes of mouse, C gamma 3, C gamma 1, C gamma 2b and C gamma 2a, together with that of a human equivalent C gamma 4 were...
Induction of skin fibrosis in mice expressing a mutated fibrillin-1 gene.
Saito, S., Nishimura, H., Phelps, R. G., Wolf, I., Suzuki, M., Honjo, T., ...
BACKGROUND: Tight skin mice (TSK) bear a mutated Fibrillin-1 (Fbn-1) gene. Genetic studies show that the TSK mutation is closely associated with the Fbn-1 locus (0-0.7 cM). A previous study showed...
Ochi, H, Takeshita, H, Suda, T, Nisitani, S, Honjo, T, Watanabe, T
The src‐family protein tyrosine kinase, Lyn, has been reported to play a crucial role in the regulation of B‐cell antigen receptor (BCR)‐mediated signalling. To elucidate the role of Lyn in the...