Production of Neutron-Rich Lambda Hypernuclei at J-PARC (2009)
Sakaguchi, A., Ajimura, S., Bhang, H., Busso, L., Endo, M., Faso, D., ...
We discuss the usefulness of the double charge-exchange reactions (DCX) for the production of the neutron-rich Lambda-hypernuclei. We believe the (pi-,K+) reaction is one of the most promising DCX...
Direct evaporative cooling of 41K into a Bose-Einstein condensate (2008)
Kishimoto, T., Kobayashi, J., Noda, K., Aikawa, K., Ueda, M., Inouye, S.
We have investigated the collisional properties of 41K atoms at ultracold temperature. To show the possibility to use 41K as a coolant, a Bose-Einstein condensate of 41K atoms in the stretched state...
Neutrino-less Double Beta Decay of 48Ca studied by CaF2(Eu) Scintillators (2008)
Umehara, S., Kishimoto, T., Ogawa, I., Hazama, R., Miyawaki, H., Yoshida, S., ...
We searched for the neutrino-less double beta decay(0nbb) of 48Ca by using CaF2(Eu) scintillators. Analysis of their pulse shapes was effective to reduce backgrounds. No events are observed in the...
Yokota, S, Imagawa, T, Miyamae, T, Kasai, K, Mori, M, Nishimoto, N, ...
No abstract available.
XI. Neuroenergetics: Relevance for Functional Brain Imaging, eds P. J. (2008)
In Eukaryotes, R. Y. Tsien, K. Mikoshiba, J. Altman, R. Caminiti, ...
The HFSP workshops were initiated by Michel Cuénod (Secretary-General, HFSPO) with the support of the Board of Trustees and the Council of Scientists. The workshops, held in English, are organized...
H. Bhang, H. Park, J. H. Kim, Y. D. Kim, M. Youn, O. Hashimoto, ...
Through the last 7-8 years of collaboration with the SKS hypernuclear physics group at KEK, we have concentrated on the measurement of hypernuclear decays. Non-mesonic weak decay mode of hypernucleus...
Challenge on Ca-48 enrichment for CANDLES double beta decay experiment (2007)
Hazama, R., Tatewaki, Y., Kishimoto, T., Matsuoka, K., Endo, N., Kume, K., ...
Chemical isotope effects of calcium were studied by liquid-liquid extraction using a crown ether of dicyclohexano-18-crown-6 for the purpose of finding a cost-effective and efficient way of...
Supriatna, Y., Kishimoto, T., Furuya, M., Tochigi, N., Ishiguro, H., Tosh, D., ...
Alpha-fetoprotein (AFP)-producing gastric cancer (AFP-GC) is a highly malignant variant of adenocarcinoma with aberrant hepatocellular phenotype. A detailed understanding of the regulation of its...
Electrodynamic trapping of spinless neutral atoms with an atom chip (2006)
Kishimoto, T., Hachisu, H., Fujiki, J., Nagato, K., Yasuda, M., Katori, H.
Three dimensional electrodynamic trapping of neutral atoms has been demonstrated. By applying time-varying inhomogeneous electric fields with micron-sized electrodes, nearly $10^2$ strontium atoms in...
Doganci, A., Eigenbrod, T., Krug, N., Sanctis, G.T. De, Hausding, M., Erpenbeck, V.J., ...
The cytokine IL-6 acts via a specific receptor complex that consists of the membrane-bound IL-6 receptor (mIL-6R) or the soluble IL-6 receptor (sIL-6R) and glycoprotein 130 (gp130). In this study, we...
Mesonic and nonmesonic weak decay widths of medium-heavy lambda hypernuclei (2004)
Sato, Y., Ajimura, S., Aoki, K., Bhang, H., Hasegawa, T., Hashimoto, O., ...
We have measured the energy spectra of pions and protons emitted in the weak decay of 12_Lambda_C, 28_Lambda_Si, and Lambda_Fe hypernuclei produced via the (pi+, K+) reaction. The decay widths of the...
Saha, P. K., Noumi, H., Abe, D., Ajimura, S., Aoki, K., Bhang, H. C., ...
In order to study the Sigma-nucleus optical potential, we measured inclusive (pi^-,K^+) spectra on medium-to-heavy nuclear targets: CH_2, Si, Ni, In and Bi. The CH_2 target was used to calibrate the...
Pathogenic roles of IL-6 and IL-6 blocking therapy on rheumatoid arthritis (2003)
Yoshizaki, K, Nishimoto, N, Miyasaka, N, Yamamoto, K, Kawai, S, Takeuchi, T, ...
No abstract available.
Choy, E.H.S., Isenberg, D.A., Garrood, T., Farrow, S., Ioannou, Y., Bird, H., ...
Objective: To investigate the safety and efficacy of MRA, a recombinant human anti-interleukin-6 (anti-IL-6) receptor monoclonal antibody of the IgG1 subclass that inhibits the function of IL-6, in...
Scintillation efficiency of nuclear recoils in a CaF-2(Eu) crystal for dark matter search (2001)
Hazama, R., Ajimura, S., Hayakawa, H., Matsuoka, K., Miyawaki, H., Morikubo, K., ...
The scintillation efficiency (quenching factor) of fluorine and calcium nuclei recoiling in a CaF-2(Eu) crystal was measured in an energy region relevant to dark matter searches. The recoiling nuclei...
Observation of Spin-Orbit Splitting in Lambda Single-Particle States (2001)
Ajimura, S., Hayakawa, H., Kishimoto, T., Kohri, H., Matsuoka, K.
WORKSHOP VIII THE REGULATION OF SLEEP EDITORS (2000)
W. Fangman, T. Kishimoto, M. Kohiyama, C. Coath, S. Ang, R. Behringer, ...
To be published:
Spin observables in the $pn \to p \Lambda$ reaction (1998)
Nabetani, H., Ogaito, T., Sato, T., Kishimoto, T.
The T matrix of the LambdaN-> NN reaction, which is a strangeness changing weak process, is derived. The explicit formulas of the spin observables are given for s-wave p-Lambda final states which...
Polarization of $^{5}_{\Lambda}He$ produced by the $(\pi^{+}, K^{+})$ reaction (1998)
Ajimura, S, Ishikawa, M, Ikeda, K, Kishimoto, T, Okusu, A, Shinkai, N, ...
Okuda, Y, Sakoda, S, Bernard, CC, Fujimura, H, Saeki, Y, Kishimoto, T, ...
Lee, CC, Masuda, C, Yamamoto, S, Wanibuchi, H, Ikemoto, S, Sugimura, K, ...
CD Antigens 1996: announcement (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, M., Moretta, L., ...
Update of CD antigens, 1996 (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, M., Moretta, L., ...
Update: new CD antigens, 1996 (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, M., Moretta, L., ...
CD antigens 1996: announcement (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, L., Moretta, L., ...
Update: new CD antigens, 1996 (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, L., Moretta, L., ...
Update of CD antigens, 1996 (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, L., Moretta, L., ...
CD Antigens 1996: announcement (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, M., Moretta, L., ...
Update of CD antigens, 1996 (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, M., Moretta, L., ...
Update: new CD antigens, 1996 (1997)
Kishimoto, T., Goyert, S., Kikutani, H., Mason, D., Miyasaka, M., Moretta, L., ...
Self-Consistent Velocity Dependent Effective Interactions (1996)
Kubo, T., Sakamoto, H., Kammuri, T., Kishimoto, T.
The theory of self-consistent effective interactions in nuclei is extended for a system with a velocity dependent mean potential. By means of the field coupling method, we present a general...
Hypernuclear weak decay of $_{\Lambda}^{12}$C and $_{\Lambda}^{11}$B (1995)
Noumi, H, Ajimura, S, Ejiri, H, Higashi, A, Kishimoto, T, Gill, D R, ...
Diagnostic radionuclide imaging of dialysis-related amyloidosis (1995)
Kiyota, A., Sugimura, T., Yamagami, S., Kishimoto, T., Shaldon, S.
MIHARA, M., MORIYA, Y., KISHIMOTO, T., OHSUGI, Y.
A number of investigators have reported that there are detectably elevated levels of interleukin-6 (IL-6) and soluble IL-6 receptor (sIL-6R) in the synovial fluids of rheumatoid arthritis (RA)...
The superconducting kaon spectrometer: SKS (1994)
Fukuda, T, Hasegawa, T, Hashimoto, O, Higashi, A, Homma, S, Kitami, T, ...
The $\Lambda$ hypernuclear spectroscopy with the SKS spectrometer at KEK 12 GeV PS (1994)
Ajimura, S, Aoki, K, Bhang, H C, Gavrilov, Yu K, Hasegawa, T, Hashimoto, O, ...
Hasegawa, T, Hashimoto, O, Homma, S, Miyachi, T, Nagae, T, Sekimoto, M, ...
K$^+$-nucleus quasielastic scattering (1993)
Kormanyos, C. M., Peterson, R. J., Shepard, J. R., Wise, J. E., Bart, S., Chrien, R. E., ...
K$^+$--nucleus quasielastic cross sections measured for a laboratory kaon beam momentum of 705 MeV/$c$ are presented for 3--momentum transfers of 300 and 500 MeV/$c$. The measured differential cross...
Behaviour of platelets and {beta}-thromboglobulin (1993)
Ivanovich, P., Hammerschmidt, D. E., Quintanilla, A., Kishimoto, T., Tanaka, H., Levin, N., ...
Platelet count, platelet aggregation responses, and plasma β-thromboglobulin were assessed before and at intervals during each study dialysis. As expected, modest thrombocytopenia was observed...
{beta}2-Microglobulin and granulocyte elastase (1993)
Wehle, B., Bergstrom, J., Kishimoto, T., Lantz, B., Levin, N., Klinkmann, H.
Plasma β2-M was measured by radio-immunoassay in samples obtained before and after dialysis with seven different dialysers, tested according to the protocol of the International Cooperative...
Dialysis-induced hypoxaemia (1993)
Kishimoto, T., Tanaka, H., Maekawa, M., Ivanovich, P., Levin, N., Bergstrom, J., ...
A crossover study to compare the effects of seven different dialysers on blood gas conditions during dialysis using acetate-containing dialysate was carried out at five centres in four countries. A...
Behaviour of white blood cells and the complement system (1993)
Falkenhagen, D., Brown, G. S., Thomaneck, U., Levin, N. W., Ivanovich, P., Bergstrom, J., ...
Seven different types of dialysers were investigated in five dialysis centres in four countries with respect to behaviour of white blood cells and the complement system. The results of this...
EXPERIMENTAL-STUDY OF A CO2-LASER PLASMA BY COHERENT ANTI-STOKES-RAMAN SCATTERING (CARS) (1992)
Kishimoto, T., Wenzel, N., Grossewilde, H., Lupke, G., Marowsky, G.
THERMAL DISEQUILIBRIUM EFFECTS IN A MOLECULAR GLOW-DISCHARGE (1992)
Lupke, G., Marowsky, G., Sieverdes, F., Wenzel, N., Kishimoto, T., Grossewilde, H.
Kurdowska, A, Koj, A, Hirano, T, Kishimoto, T, Content, J, ...
Sasaki, K, Yagi, H, Bronson, R T, Tominaga, K, Matsunashi, T, Deguchi, K, ...
Core binding factor beta (CBF beta) is considered to be a transcriptional coactivator that dimerizes with transcription factors core binding factor alpha 1 (CBFA1), -2, and -3, and enhances DNA...
Minami, M, Inoue, M, Wei, S, Takeda, K, Matsumoto, M, Kishimoto, T, ...
Myeloid leukemia M1 cells can be induced for growth arrest and terminal differentiation into macrophages in response to interleukin 6 (IL-6) or leukemia inhibitory factor (LIF). Recently, a large...
Yoshida, K, Taga, T, Saito, M, Suematsu, S, Kumanogoh, A, Tanaka, T, ...
gp130 is a ubiquitously expressed signal-transducing receptor component shared by interleukin 6, interleukin 11, leukemia inhibitory factor, oncostatin M, ciliary neurotrophic factor, and...
Hirota, H, Yoshida, K, Kishimoto, T, Taga, T
To investigate the physiological roles of gp130 in detail and to determine the pathological consequence of abnormal activation of gp130, transgenic mice having continuously activated gp130 were...
Sui, X, Tsuji, K, Tanaka, R, Tajima, S, Muraoka, K, Ebihara, Y, ...
gp130, a signal-transducing receptor component of interleukin 6 (IL-6), associates with an IL-6 and IL-6 receptor (IL-6) complex and transduces signals. To examine the role of gp130 signaling in the...
A sauvagine/corticotropin-releasing factor receptor expressed in heart and skeletal muscle.
Kishimoto, T, Pearse, R V, Lin, C R, Rosenfeld, M G
Corticotropin-releasing factor (CRF) mediates many critical aspects of the physiological response to stress. These effects are elicited by binding to specific high-affinity receptors, which are...
Activation of JAK2 kinase mediated by the interleukin 6 signal transducer gp130.
Narazaki, M, Witthuhn, B A, Yoshida, K, Silvennoinen, O, Yasukawa, K, Ihle, J N, ...
The interleukin 6 receptor-associated signal transducer, gp130, is shared by receptor complexes for leukemia inhibitory factor, oncostatin M, ciliary neurotrophic factor, and interleukin 11. We show...
Molecular cloning and structure of a pre-B-cell growth-stimulating factor.
Nagasawa, T, Kikutani, H, Kishimoto, T
Generation and proliferation of early B-cell progenitors have been known to require stromal cell-derived molecules. A stromal cell line, PA6, was found to produce a soluble mediator, which was...
Nakajima, T, Kinoshita, S, Sasagawa, T, Sasaki, K, Naruto, M, Kishimoto, T, ...
NF-IL6, a member of the basic leucine zipper (bZIP) family transcription factors, is involved in expression of inducible genes involved in immune and inflammatory responses. We observed that...
Matsusaka, T, Fujikawa, K, Nishio, Y, Mukaida, N, Matsushima, K, Kishimoto, T, ...
Single binding sites for transcription factors NF-IL6 and NF-kappa B are present in the promoter of the interleukin (IL) 6 gene. Previous studies of internally deleted promoter mutants demonstrated...
Suematsu, S, Matsusaka, T, Matsuda, T, Ohno, S, Miyazaki, J, Yamamura, K, ...
The mechanisms through which pristane or mineral oil can induce plasmacytomas in BALB/c or NZB mice are not fully understood, but involvement of interleukin 6 (IL-6), a growth factor for...
Kinoshita, S, Akira, S, Kishimoto, T
Using a DNA probe from the DNA-binding portion of the NF-IL6 gene and an antibody against the DNA-binding domain of NF-IL6, we isolated a gene homologous to NF-IL6 in the DNA-binding and leucine...
Yokota, A, Yukawa, K, Yamamoto, A, Sugiyama, K, Suemura, M, Tashiro, Y, ...
We have previously identified two species of the low-affinity human Fc receptor for IgE, Fc epsilon RIIa and Fc epsilon RIIb, which differ only in a short stretch of amino acids at the N-terminal...
Taga, T, Narazaki, M, Yasukawa, K, Saito, T, Miki, D, Hamaguchi, M, ...
Functional pleiotropy and redundancy are characteristic features of cytokines. To understand the signaling mechanisms of such cytokines, we have proposed a two-chain interleukin (IL) 6 receptor...
Murakami, M, Narazaki, M, Hibi, M, Yawata, H, Yasukawa, K, Hamaguchi, M, ...
Interleukin 6 (IL-6) signal is transduced through gp130 that associates with a complex of IL-6 and IL-6 receptor. Truncations or amino acid substitutions offe introduced in the cytoplasmic region of...
AIDS Kaposi sarcoma-derived cells produce and respond to interleukin 6.
Miles, S A, Rezai, A R, Salazar-González, J F, Vander Meyden, M, Stevens, R H, Logan, D M, ...
Cell lines derived from Kaposi sarcoma lesions of patients with AIDS (AIDS-KS cells) produce several cytokines, including an endothelial cell growth factor, interleukin 1 beta, and basic fibroblast...
In vitro and in vivo activities of AM-1155, a new fluoroquinolone, against Chlamydia spp.
Miyashita, N, Niki, Y, Kishimoto, T, Nakajima, M, Matsushima, T
The in vitro and in vivo activities of AM-1155, a new quinolone, against Chlamydia spp. were investigated. The MIC of AM-1155 for 10 standard strains of different Chlamydia spp. and 25 wild-type...
Murakami-Mori, K, Taga, T, Kishimoto, T, Nakamura, S
Oncostatin M (OM), which shares functional similarity and structural homology to leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), functions as a potent growth factor for AIDS-associated...
Mima, T, Saeki, Y, Ohshima, S, Nishimoto, N, Matsushita, M, Shimizu, M, ...
To investigate the pathogenicity of T cells infiltrating in the rheumatoid joints, mononuclear cells (MNC), predominantly T cells, isolated from either synovial fluid or synovial tissues of the...
In vitro and in vivo antichlamydial activities of newly developed quinolone antimicrobial agents.
Kimura, M, Kishimoto, T, Niki, Y, Soejima, R
The in vitro and in vivo activities of three newly developed quinolone antimicrobial agents (sparfloxacin, tosufloxacin, and OPC-17116) were investigated. All three agents showed potent in vitro...
Degradation of c-Fos by the 26S proteasome is accelerated by c-Jun and multiple protein kinases.
Tsurumi, C, Ishida, N, Tamura, T, Kakizuka, A, Nishida, E, Okumura, E, ...
c-Fos is associated with c-Jun to increase the transcription of a number of target genes and is a nuclear proto-oncoprotein with a very short half-life. This instability of c-Fos may be important in...
NF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.
Spergel, J M, Hsu, W, Akira, S, Thimmappaya, B, Kishimoto, T, Chen-Kiang, S
A cDNA encoding NF-IL6, an interleukin-6 (IL-6)-regulated human nuclear factor of the C/EBP family, is demonstrated to complement the transactivation function of E1A. The endogenous NF-IL6 level...
Epstein-Barr virus nuclear protein 2 transactivates a cis-acting CD23 DNA element.
Wang, F, Kikutani, H, Tsang, S F, Kishimoto, T, Kieff, E
Epstein-Barr virus (EBV) nuclear protein 2 (EBNA-2) is essential for B-lymphocyte growth transformation. EBNA-2 upregulates mRNAs encoding CD23, a B-lymphocyte surface protein closely associated with...
Characterization of Chlamydia pneumoniae species-specific proteins immunodominant in humans.
Iijima, Y, Miyashita, N, Kishimoto, T, Kanamoto, Y, Soejima, R, Matsumoto, A
Proteins of Chlamydia pneumoniae immunodominant in humans were characterized with the sera of 13 patients who were not likely to have been exposed to C. trachomatis or C. psittaci. The serological...
Le, J M, Fredrickson, G, Reis, L F, Diamantstein, T, Hirano, T, Kishimoto, T, ...
Recombinant human interleukin 6 (IL-6), also termed B-cell-stimulatory factor 2 (BSF-2) or interferon-beta 2, was found to stimulate the proliferation of mouse thymocytes costimulated with...
Leary, A G, Hirai, Y, Kishimoto, T, Clark, S C, Ogawa, M
Although it is generally held that hemopoietic stem cells in steady-state marrow are dormant in the cell cycle, the direct proof for this concept has been lacking. In the present study, we have...
Fujihashi, K, McGhee, J R, Lue, C, Beagley, K W, Taga, T, Hirano, T, ...
Past studies have shown that freshly isolated human B cells from peripheral blood and tonsils do not express IL-6 receptors (IL-6R); however, mitogen or antigen activation of these B cells induces...
Yamanashi, Y, Mori, S, Yoshida, M, Kishimoto, T, Inoue, K, Yamamoto, T, ...
This paper reports the identification of the lyn gene product, a member of the src-related family of protein-tyrosine kinases, and its expression in hematopoietic cells. A lyn-specific sequence...
IgG1 plasmacytosis in interleukin 6 transgenic mice.
Suematsu, S, Matsuda, T, Aozasa, K, Akira, S, Nakano, N, Ohno, S, ...
Interleukin 6 (IL-6) has been suggested to be involved in the pathogenesis of polyclonal and monoclonal plasma cell abnormalities. To address this possibility, transgenic mice carrying the human IL-6...
Raynal, M C, Liu, Z Y, Hirano, T, Mayer, L, Kishimoto, T, Chen-Kiang, S
The molecular mechanism by which interleukin 6 (IL-6) induces terminal differentiation of B cells was investigated in a subpopulation of the clonal human B-lymphoblastoid cell line CESS selected for...
Desialation of transferrin by rat liver endothelium.
Irie, S, Kishimoto, T, Tavassoli, M
To examine the role of liver endothelium in desialation of transferrin (TF), pulse-chase studies were done by incubation of either 3H (sialic acid labeled)-, or 125I, or 59Fe (protein core...
Isolation of high-affinity memory B cells: phycoerythrin as a probe for antigen-binding cells.
Hayakawa, K, Ishii, R, Yamasaki, K, Kishimoto, T, Hardy, R R
In multiparameter fluorescence-activated cell sorter studies presented here, we use the highly fluorescent protein phycoerythrin (PE) as an immunizing antigen and as a fluorescent probe to reveal and...
Wang, F, Gregory, C D, Rowe, M, Rickinson, A B, Wang, D, Birkenbach, M, ...
Epstein-Barr virus (EBV) infection of EBV-negative Burkitt lymphoma (BL) cells induces some changes similar to those seen in normal B lymphocytes that have been growth transformed by EBV. The role of...
Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome.
Sumita, K, Makino, Y, Katoh, K, Kishimoto, T, Muramatsu, M, Mikoshiba, K, ...
Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster.
Nishida, Y, Kataoka, T, Ishida, N, Nakai, S, Kishimoto, T, Böttcher, I, ...
Mouse immunoglobulin epsilon chain gene was cloned from DNA of a hybridoma producing anti-dinitrophenyl IgE, which was constructed by fusing a spleen cell of a BALB/c mouse with a variant clone of...
The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
Yaoita, Y, Matsunami, N, Choi, C Y, Sugiyama, H, Kishimoto, T, Honjo, T
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of...
Kishimoto, T, Nagamine, M, Sasaki, T, Takakusa, N, Miwa, T, Kominami, R, ...
Point mutations are introduced into a mouse rDNA fragment containing the promoter region by a sodium bisulfite method and the mutants are tested for the ability of accurate transcription initiation...
A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.
Nakanishi, K, Sugimura, K, Yaoita, Y, Maeda, K, Kashiwamura, S, Honjo, T, ...
The T suppressive factor (TsF) released from a T15-idiotype-positive phosphocholine (PCho)-specific T hybridoma, F18-3-4, which was formed by fusion between BALB/c T cells and BW5147 thymoma, was...
Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules.
Okada, M, Yoshimura, N, Kaieda, T, Yamamura, Y, Kishimoto, T
Hybridization of human T cells with an azaguanine-resistant human T cell line gave rise to T hybrid cell lines secreting several immunoregulatory molecules. Analyses of karyotypes, HLA phenotypes,...
Nakatsuji, Y, Hidaka, K, Tsujino, S, Yamamoto, Y, Mukai, T, Yanagihara, T, ...
In order to analyze the transcriptional regulation of the muscle-specific subunit of the human phosphoglycerate mutase (PGAM-M) gene, chimeric genes composed of the upstream region of the PGAM-M gene...
Isshiki, H, Akira, S, Tanabe, O, Nakajima, T, Shimamoto, T, Hirano, T, ...
The interleukin-6 (IL-6) promoter is rapidly and transiently activated with other cytokines, including IL-1, tumor necrosis factor, and platelet-derived growth factor, as well as phorbol esters and...
Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.
Safrany, G, Tanaka, N, Kishimoto, T, Ishikawa, Y, Kato, H, Kominami, R, ...
Mammalian ribosomal DNA (rDNA) transcription has a certain species specificity such that, both in vivo and in vitro, human rDNA cannot be transcribed by mouse machinery and vice versa. This is due to...
Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.
Satoh, T, Nakamura, S, Taga, T, Matsuda, T, Hirano, T, Kishimoto, T, ...
B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is...
Nagamine, M, Kishimoto, T, Aono, J, Kato, H, Kominami, R, Muramatsu, M
We compared the ability of various deletion and substitution mutants of the mouse rRNA gene promoter to bind essential factors required for accurate transcription initiation by RNA polymerase I....
Immunoglobulin production by lymphoblast cell lines was studies using protein A-red blood cell plaque formation to detect individual secreting cells. Immunoglobulin (Ig) secretion by 6 of 12 human...
Hirano, T, Taga, T, Nakano, N, Yasukawa, K, Kashiwamura, S, Shimizu, K, ...
Human B-cell differentiation factor (BCDF) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from TCL-Na1 cells on an AcA34 gel column and then on a Mono...
Saeki, Y, Mima, T, Sakoda, S, Fujimura, H, Arita, N, Nomura, T, ...
To investigate the mode of the pathogenesis of multiple sclerosis (MS), we transferred cerebrospinal fluid (CSF) cells, predominantly mononuclear cells, from MS patients at both exacerbation and...
Yawata, H, Yasukawa, K, Natsuka, S, Murakami, M, Yamasaki, K, Hibi, M, ...
Here, we report the analysis of the structure-function relationship of the extracellular region of human interleukin 6 receptor (IL-6R). Upon binding of IL-6, IL-6R becomes associated extracellularly...
Kawamura, N, Muraguchi, A, Hori, A, Horii, Y, Mutsuura, S, Hardy, R R, ...
Production of B cell growth factor (BCGF) from B-chronic lymphocytic leukemia (B-CLL) cells was demonstrated. Freshly isolated monoclonal B-CLL cells expressed surface mu, delta, B1, and Leu 1, but...
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
Gotoh, Y, Moriyama, K, Matsuda, S, Okumura, E, Kishimoto, T, Kawasaki, H, ...
MAP kinase is activated and phosphorylated during M phase of the Xenopus oocyte cell cycle, and induces the interphase-M phase transition of microtubule dynamics in vitro. We have carried out...
Yamamoto, T, Ozono, K, Kasayama, S, Yoh, K, Hiroshima, K, Takagi, M, ...
McCune-Albright syndrome (MAS) is characterized by café-au-lait spot, multiple endocrine hyperfunction, and polyostotic fibrous dysplasia. A somatic point mutation of Gsalpha protein was reported to...
Fujio, Y, Kunisada, K, Hirota, H, Yamauchi-Takihara, K, Kishimoto, T
We described recently the activation of the Janus kinasesignal transducer and activator of transcription (JakSTAT) and mitogen-activated protein (MAP) kinase pathways by leukemia inhibitory factor...
Multimerization of the mouse TATA-binding protein (TBP) driven by its C-terminal conserved domain.
Kato, K, Makino, Y, Kishimoto, T, Yamauchi, J, Kato, S, Muramatsu, M, ...
The conformational states of the mouse TATA-binding protein (TBP) in solution were studied. A histidine tag and a factor Xa recognition site-carrying mouse TBP was expressed in E. coli, highly...
Oster, W, Cicco, N A, Klein, H, Hirano, T, Kishimoto, T, Lindemann, A, ...
Autonomous in vitro growth of myeloid leukemic colony-forming cells may in part result from autocrine production of colony-stimulating factors (CSF). Some acute myeloid leukemia (AML) samples,...
Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene.
Yasukawa, K, Hirano, T, Watanabe, Y, Muratani, K, Matsuda, T, Nakai, S, ...
The chromosomal DNA segment of human B cell stimulatory factor-2 (BSF-2/IL-6) was isolated and characterized by nucleotide sequence analysis. The human BSF-2/IL-6 gene consists of five exons and four...
Ookata, K, Hisanaga, S, Okano, T, Tachibana, K, Kishimoto, T
M phase promoting factor (MPF) is a major element controlling entry into the M phase of the eukaryotic cell cycle. MPF is composed of two subunits, p34cdc2 and cyclin B. Using indirect...
Immunoglobulin gene expression and DNA methylation in murine pre-B cell lines.
Akira, S, Sugiyama, H, Sakaguchi, N, Kishimoto, T
DNA modification accompanying immunoglobulin gene expression was examined in various Abelson murine leukemia virus (A-MuLV)-transformed cell lines, which were able to differentiate from the mu- to...
Kinetic diversity in the fusion of exocytotic vesicles.
Ninomiya, Y, Kishimoto, T, Yamazawa, T, Ikeda, H, Miyashita, Y, Kasai, H
The speed at which secretory vesicles fuse with the plasma membrane is a key parameter for neuronal and endocrine functions. We determined the precise time courses for fusion of small clear and large...
Tachibana, K, Machida, T, Nomura, Y, Kishimoto, T
The mechanism by which fertilization initiates S-phase in the zygote is examined by manipulating the activity of MAP kinase in mature starfish eggs. These unfertilized eggs, which are arrested at...
Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene.
Tanaka, T, Yoshida, N, Kishimoto, T, Akira, S
To investigate the role of C/EBP family members during adipocyte differentiation in vivo, we have generated mice lacking the C/EBPbeta and/or C/EBPdelta by gene targeting. Approximately 85% of...
Kamiya, A, Kinoshita, T, Ito, Y, Matsui, T, Morikawa, Y, Senba, E, ...
Fetal liver, the major site of hematopoiesis during embryonic development, acquires additional various metabolic functions near birth. Although liver development has been characterized biologically...
Interleukin 6 activates heat-shock protein 90 beta gene expression.
Stephanou, A, Amin, V, Isenberg, D A, Akira, S, Kishimoto, T, Latchman, D S
The levels of the cytokine interleukin-6 (IL-6) and the heat-shock protein hsp90 have both been reported to be elevated in patients with active systemic lupus erythematosus (SLE). We show that hsp90...
Stephanou, A, Isenberg, D A, Akira, S, Kishimoto, T, Latchman, D S
The levels of the 90 kDa heat-shock protein (hsp90) and the activity of the hsp90beta gene promoter are increased in response to treatment by interleukin (IL)-6. The hsp90beta gene promoter contains...
New Criteria for Immunofluorescence Assay for Q Fever Diagnosis in Japan
Setiyono, A., Ogawa, M., Cai, Y., Shiga, S., Kishimoto, T., Kurane, I.
A study was made to evaluate the cutoff value of indirect immunofluorescent-antibody (IFA) test for Q fever diagnosis in Japan. We used 346 sera, including 16 from confirmed Q fever cases, 304 from...
Nishio, Y, Isshiki, H, Kishimoto, T, Akira, S
The acute-phase reaction is accompanied by an increase in a variety of serum proteins, named acute-phase proteins. The synthesis of these proteins is synergistically controlled by glucocorticoids and...
A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family.
Akira, S, Isshiki, H, Sugita, T, Tanabe, O, Kinoshita, S, Nishio, Y, ...
NF-IL6 is a nuclear factor that specifically binds to an IL1-responsive element in the IL-6 gene. In this study the gene encoding NF-IL6 has been cloned by direct screening of a lambda gt11 library...
Schooltink, H, Stoyan, T, Lenz, D, Schmitz, H, Hirano, T, Kishimoto, T, ...
cDNAs coding for the human hepatic interleukin-6 receptor (IL-6-R) have been isolated from a library made from poly(A) RNA of dexamethasone-treated human hepatoma cells (HepG2). We found the hepatic...
Kasai, H, Takagi, H, Ninomiya, Y, Kishimoto, T, Ito, K, Yoshida, A, ...
1. Changes in membrane capacitance evoked by the rapid photolysis of a caged Ca2+ compound, DM-nitrophen or nitrophenyl-EGTA, were investigated in undifferentiated PC12 cells. They were interpreted...
Sasaki, K, Yagi, H, Bronson, R T, Tominaga, K, Matsunashi, T, Deguchi, K, ...
Core binding factor beta (CBF beta) is considered to be a transcriptional coactivator that dimerizes with transcription factors core binding factor alpha 1 (CBFA1), -2, and -3, and enhances DNA...
Minami, M, Inoue, M, Wei, S, Takeda, K, Matsumoto, M, Kishimoto, T, ...
Myeloid leukemia M1 cells can be induced for growth arrest and terminal differentiation into macrophages in response to interleukin 6 (IL-6) or leukemia inhibitory factor (LIF). Recently, a large...
Yoshida, K, Taga, T, Saito, M, Suematsu, S, Kumanogoh, A, Tanaka, T, ...
gp130 is a ubiquitously expressed signal-transducing receptor component shared by interleukin 6, interleukin 11, leukemia inhibitory factor, oncostatin M, ciliary neurotrophic factor, and...
Hirota, H, Yoshida, K, Kishimoto, T, Taga, T
To investigate the physiological roles of gp130 in detail and to determine the pathological consequence of abnormal activation of gp130, transgenic mice having continuously activated gp130 were...
Sui, X, Tsuji, K, Tanaka, R, Tajima, S, Muraoka, K, Ebihara, Y, ...
gp130, a signal-transducing receptor component of interleukin 6 (IL-6), associates with an IL-6 and IL-6 receptor (IL-6) complex and transduces signals. To examine the role of gp130 signaling in the...
A sauvagine/corticotropin-releasing factor receptor expressed in heart and skeletal muscle.
Kishimoto, T, Pearse, R V, Lin, C R, Rosenfeld, M G
Corticotropin-releasing factor (CRF) mediates many critical aspects of the physiological response to stress. These effects are elicited by binding to specific high-affinity receptors, which are...
Activation of JAK2 kinase mediated by the interleukin 6 signal transducer gp130.
Narazaki, M, Witthuhn, B A, Yoshida, K, Silvennoinen, O, Yasukawa, K, Ihle, J N, ...
The interleukin 6 receptor-associated signal transducer, gp130, is shared by receptor complexes for leukemia inhibitory factor, oncostatin M, ciliary neurotrophic factor, and interleukin 11. We show...
Molecular cloning and structure of a pre-B-cell growth-stimulating factor.
Nagasawa, T, Kikutani, H, Kishimoto, T
Generation and proliferation of early B-cell progenitors have been known to require stromal cell-derived molecules. A stromal cell line, PA6, was found to produce a soluble mediator, which was...
Nakajima, T, Kinoshita, S, Sasagawa, T, Sasaki, K, Naruto, M, Kishimoto, T, ...
NF-IL6, a member of the basic leucine zipper (bZIP) family transcription factors, is involved in expression of inducible genes involved in immune and inflammatory responses. We observed that...
Matsusaka, T, Fujikawa, K, Nishio, Y, Mukaida, N, Matsushima, K, Kishimoto, T, ...
Single binding sites for transcription factors NF-IL6 and NF-kappa B are present in the promoter of the interleukin (IL) 6 gene. Previous studies of internally deleted promoter mutants demonstrated...
Suematsu, S, Matsusaka, T, Matsuda, T, Ohno, S, Miyazaki, J, Yamamura, K, ...
The mechanisms through which pristane or mineral oil can induce plasmacytomas in BALB/c or NZB mice are not fully understood, but involvement of interleukin 6 (IL-6), a growth factor for...
Kinoshita, S, Akira, S, Kishimoto, T
Using a DNA probe from the DNA-binding portion of the NF-IL6 gene and an antibody against the DNA-binding domain of NF-IL6, we isolated a gene homologous to NF-IL6 in the DNA-binding and leucine...
Yokota, A, Yukawa, K, Yamamoto, A, Sugiyama, K, Suemura, M, Tashiro, Y, ...
We have previously identified two species of the low-affinity human Fc receptor for IgE, Fc epsilon RIIa and Fc epsilon RIIb, which differ only in a short stretch of amino acids at the N-terminal...
Taga, T, Narazaki, M, Yasukawa, K, Saito, T, Miki, D, Hamaguchi, M, ...
Functional pleiotropy and redundancy are characteristic features of cytokines. To understand the signaling mechanisms of such cytokines, we have proposed a two-chain interleukin (IL) 6 receptor...
Murakami, M, Narazaki, M, Hibi, M, Yawata, H, Yasukawa, K, Hamaguchi, M, ...
Interleukin 6 (IL-6) signal is transduced through gp130 that associates with a complex of IL-6 and IL-6 receptor. Truncations or amino acid substitutions offe introduced in the cytoplasmic region of...
AIDS Kaposi sarcoma-derived cells produce and respond to interleukin 6.
Miles, S A, Rezai, A R, Salazar-González, J F, Vander Meyden, M, Stevens, R H, Logan, D M, ...
Cell lines derived from Kaposi sarcoma lesions of patients with AIDS (AIDS-KS cells) produce several cytokines, including an endothelial cell growth factor, interleukin 1 beta, and basic fibroblast...
In vitro and in vivo activities of AM-1155, a new fluoroquinolone, against Chlamydia spp.
Miyashita, N, Niki, Y, Kishimoto, T, Nakajima, M, Matsushima, T
The in vitro and in vivo activities of AM-1155, a new quinolone, against Chlamydia spp. were investigated. The MIC of AM-1155 for 10 standard strains of different Chlamydia spp. and 25 wild-type...
Murakami-Mori, K, Taga, T, Kishimoto, T, Nakamura, S
Oncostatin M (OM), which shares functional similarity and structural homology to leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), functions as a potent growth factor for AIDS-associated...
Mima, T, Saeki, Y, Ohshima, S, Nishimoto, N, Matsushita, M, Shimizu, M, ...
To investigate the pathogenicity of T cells infiltrating in the rheumatoid joints, mononuclear cells (MNC), predominantly T cells, isolated from either synovial fluid or synovial tissues of the...
In vitro and in vivo antichlamydial activities of newly developed quinolone antimicrobial agents.
Kimura, M, Kishimoto, T, Niki, Y, Soejima, R
The in vitro and in vivo activities of three newly developed quinolone antimicrobial agents (sparfloxacin, tosufloxacin, and OPC-17116) were investigated. All three agents showed potent in vitro...
Degradation of c-Fos by the 26S proteasome is accelerated by c-Jun and multiple protein kinases.
Tsurumi, C, Ishida, N, Tamura, T, Kakizuka, A, Nishida, E, Okumura, E, ...
c-Fos is associated with c-Jun to increase the transcription of a number of target genes and is a nuclear proto-oncoprotein with a very short half-life. This instability of c-Fos may be important in...
NF-IL6, a member of the C/EBP family, regulates E1A-responsive promoters in the absence of E1A.
Spergel, J M, Hsu, W, Akira, S, Thimmappaya, B, Kishimoto, T, Chen-Kiang, S
A cDNA encoding NF-IL6, an interleukin-6 (IL-6)-regulated human nuclear factor of the C/EBP family, is demonstrated to complement the transactivation function of E1A. The endogenous NF-IL6 level...
Epstein-Barr virus nuclear protein 2 transactivates a cis-acting CD23 DNA element.
Wang, F, Kikutani, H, Tsang, S F, Kishimoto, T, Kieff, E
Epstein-Barr virus (EBV) nuclear protein 2 (EBNA-2) is essential for B-lymphocyte growth transformation. EBNA-2 upregulates mRNAs encoding CD23, a B-lymphocyte surface protein closely associated with...
Characterization of Chlamydia pneumoniae species-specific proteins immunodominant in humans.
Iijima, Y, Miyashita, N, Kishimoto, T, Kanamoto, Y, Soejima, R, Matsumoto, A
Proteins of Chlamydia pneumoniae immunodominant in humans were characterized with the sera of 13 patients who were not likely to have been exposed to C. trachomatis or C. psittaci. The serological...
Le, J M, Fredrickson, G, Reis, L F, Diamantstein, T, Hirano, T, Kishimoto, T, ...
Recombinant human interleukin 6 (IL-6), also termed B-cell-stimulatory factor 2 (BSF-2) or interferon-beta 2, was found to stimulate the proliferation of mouse thymocytes costimulated with...
Leary, A G, Hirai, Y, Kishimoto, T, Clark, S C, Ogawa, M
Although it is generally held that hemopoietic stem cells in steady-state marrow are dormant in the cell cycle, the direct proof for this concept has been lacking. In the present study, we have...
Fujihashi, K, McGhee, J R, Lue, C, Beagley, K W, Taga, T, Hirano, T, ...
Past studies have shown that freshly isolated human B cells from peripheral blood and tonsils do not express IL-6 receptors (IL-6R); however, mitogen or antigen activation of these B cells induces...
Yamanashi, Y, Mori, S, Yoshida, M, Kishimoto, T, Inoue, K, Yamamoto, T, ...
This paper reports the identification of the lyn gene product, a member of the src-related family of protein-tyrosine kinases, and its expression in hematopoietic cells. A lyn-specific sequence...
IgG1 plasmacytosis in interleukin 6 transgenic mice.
Suematsu, S, Matsuda, T, Aozasa, K, Akira, S, Nakano, N, Ohno, S, ...
Interleukin 6 (IL-6) has been suggested to be involved in the pathogenesis of polyclonal and monoclonal plasma cell abnormalities. To address this possibility, transgenic mice carrying the human IL-6...
Raynal, M C, Liu, Z Y, Hirano, T, Mayer, L, Kishimoto, T, Chen-Kiang, S
The molecular mechanism by which interleukin 6 (IL-6) induces terminal differentiation of B cells was investigated in a subpopulation of the clonal human B-lymphoblastoid cell line CESS selected for...
Desialation of transferrin by rat liver endothelium.
Irie, S, Kishimoto, T, Tavassoli, M
To examine the role of liver endothelium in desialation of transferrin (TF), pulse-chase studies were done by incubation of either 3H (sialic acid labeled)-, or 125I, or 59Fe (protein core...
Isolation of high-affinity memory B cells: phycoerythrin as a probe for antigen-binding cells.
Hayakawa, K, Ishii, R, Yamasaki, K, Kishimoto, T, Hardy, R R
In multiparameter fluorescence-activated cell sorter studies presented here, we use the highly fluorescent protein phycoerythrin (PE) as an immunizing antigen and as a fluorescent probe to reveal and...
Wang, F, Gregory, C D, Rowe, M, Rickinson, A B, Wang, D, Birkenbach, M, ...
Epstein-Barr virus (EBV) infection of EBV-negative Burkitt lymphoma (BL) cells induces some changes similar to those seen in normal B lymphocytes that have been growth transformed by EBV. The role of...
Structure of a mammalian TBP (TATA-binding protein) gene: isolation of the mouse TBP genome.
Sumita, K, Makino, Y, Katoh, K, Kishimoto, T, Muramatsu, M, Mikoshiba, K, ...
Cloning of mouse immunoglobulin epsilon gene and its location within the heavy chain gene cluster.
Nishida, Y, Kataoka, T, Ishida, N, Nakai, S, Kishimoto, T, Böttcher, I, ...
Mouse immunoglobulin epsilon chain gene was cloned from DNA of a hybridoma producing anti-dinitrophenyl IgE, which was constructed by fusing a spleen cell of a BALB/c mouse with a variant clone of...
The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.
Yaoita, Y, Matsunami, N, Choi, C Y, Sugiyama, H, Kishimoto, T, Honjo, T
We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of...
Kishimoto, T, Nagamine, M, Sasaki, T, Takakusa, N, Miwa, T, Kominami, R, ...
Point mutations are introduced into a mouse rDNA fragment containing the promoter region by a sodium bisulfite method and the mutants are tested for the ability of accurate transcription initiation...
A T15-idiotype-positive T suppressor hybridoma does not use the T15 VH gene segment.
Nakanishi, K, Sugimura, K, Yaoita, Y, Maeda, K, Kashiwamura, S, Honjo, T, ...
The T suppressive factor (TsF) released from a T15-idiotype-positive phosphocholine (PCho)-specific T hybridoma, F18-3-4, which was formed by fusion between BALB/c T cells and BW5147 thymoma, was...
Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules.
Okada, M, Yoshimura, N, Kaieda, T, Yamamura, Y, Kishimoto, T
Hybridization of human T cells with an azaguanine-resistant human T cell line gave rise to T hybrid cell lines secreting several immunoregulatory molecules. Analyses of karyotypes, HLA phenotypes,...
Nishio, Y, Isshiki, H, Kishimoto, T, Akira, S
The acute-phase reaction is accompanied by an increase in a variety of serum proteins, named acute-phase proteins. The synthesis of these proteins is synergistically controlled by glucocorticoids and...
Nakatsuji, Y, Hidaka, K, Tsujino, S, Yamamoto, Y, Mukai, T, Yanagihara, T, ...
In order to analyze the transcriptional regulation of the muscle-specific subunit of the human phosphoglycerate mutase (PGAM-M) gene, chimeric genes composed of the upstream region of the PGAM-M gene...
Isshiki, H, Akira, S, Tanabe, O, Nakajima, T, Shimamoto, T, Hirano, T, ...
The interleukin-6 (IL-6) promoter is rapidly and transiently activated with other cytokines, including IL-1, tumor necrosis factor, and platelet-derived growth factor, as well as phorbol esters and...
Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.
Safrany, G, Tanaka, N, Kishimoto, T, Ishikawa, Y, Kato, H, Kominami, R, ...
Mammalian ribosomal DNA (rDNA) transcription has a certain species specificity such that, both in vivo and in vitro, human rDNA cannot be transcribed by mouse machinery and vice versa. This is due to...
Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.
Satoh, T, Nakamura, S, Taga, T, Matsuda, T, Hirano, T, Kishimoto, T, ...
B-cell stimulatory factor 2 (BSF-2) is a lymphokine which induces the final maturation of B cells. BSF-2 acts on a variety of cells other than B cells, and moreover, expression of BSF-2 mRNA is...
Nagamine, M, Kishimoto, T, Aono, J, Kato, H, Kominami, R, Muramatsu, M
We compared the ability of various deletion and substitution mutants of the mouse rRNA gene promoter to bind essential factors required for accurate transcription initiation by RNA polymerase I....
Immunoglobulin production by lymphoblast cell lines was studies using protein A-red blood cell plaque formation to detect individual secreting cells. Immunoglobulin (Ig) secretion by 6 of 12 human...
Hirano, T, Taga, T, Nakano, N, Yasukawa, K, Kashiwamura, S, Shimizu, K, ...
Human B-cell differentiation factor (BCDF) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from TCL-Na1 cells on an AcA34 gel column and then on a Mono...
Saeki, Y, Mima, T, Sakoda, S, Fujimura, H, Arita, N, Nomura, T, ...
To investigate the mode of the pathogenesis of multiple sclerosis (MS), we transferred cerebrospinal fluid (CSF) cells, predominantly mononuclear cells, from MS patients at both exacerbation and...
Yawata, H, Yasukawa, K, Natsuka, S, Murakami, M, Yamasaki, K, Hibi, M, ...
Here, we report the analysis of the structure-function relationship of the extracellular region of human interleukin 6 receptor (IL-6R). Upon binding of IL-6, IL-6R becomes associated extracellularly...
Kawamura, N, Muraguchi, A, Hori, A, Horii, Y, Mutsuura, S, Hardy, R R, ...
Production of B cell growth factor (BCGF) from B-chronic lymphocytic leukemia (B-CLL) cells was demonstrated. Freshly isolated monoclonal B-CLL cells expressed surface mu, delta, B1, and Leu 1, but...
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
Gotoh, Y, Moriyama, K, Matsuda, S, Okumura, E, Kishimoto, T, Kawasaki, H, ...
MAP kinase is activated and phosphorylated during M phase of the Xenopus oocyte cell cycle, and induces the interphase-M phase transition of microtubule dynamics in vitro. We have carried out...
Yamamoto, T, Ozono, K, Kasayama, S, Yoh, K, Hiroshima, K, Takagi, M, ...
McCune-Albright syndrome (MAS) is characterized by café-au-lait spot, multiple endocrine hyperfunction, and polyostotic fibrous dysplasia. A somatic point mutation of Gsalpha protein was reported to...
Fujio, Y, Kunisada, K, Hirota, H, Yamauchi-Takihara, K, Kishimoto, T
We described recently the activation of the Janus kinasesignal transducer and activator of transcription (JakSTAT) and mitogen-activated protein (MAP) kinase pathways by leukemia inhibitory factor...
Multimerization of the mouse TATA-binding protein (TBP) driven by its C-terminal conserved domain.
Kato, K, Makino, Y, Kishimoto, T, Yamauchi, J, Kato, S, Muramatsu, M, ...
The conformational states of the mouse TATA-binding protein (TBP) in solution were studied. A histidine tag and a factor Xa recognition site-carrying mouse TBP was expressed in E. coli, highly...
Oster, W, Cicco, N A, Klein, H, Hirano, T, Kishimoto, T, Lindemann, A, ...
Autonomous in vitro growth of myeloid leukemic colony-forming cells may in part result from autocrine production of colony-stimulating factors (CSF). Some acute myeloid leukemia (AML) samples,...
A nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family.
Akira, S, Isshiki, H, Sugita, T, Tanabe, O, Kinoshita, S, Nishio, Y, ...
NF-IL6 is a nuclear factor that specifically binds to an IL1-responsive element in the IL-6 gene. In this study the gene encoding NF-IL6 has been cloned by direct screening of a lambda gt11 library...
Structure and expression of human B cell stimulatory factor-2 (BSF-2/IL-6) gene.
Yasukawa, K, Hirano, T, Watanabe, Y, Muratani, K, Matsuda, T, Nakai, S, ...
The chromosomal DNA segment of human B cell stimulatory factor-2 (BSF-2/IL-6) was isolated and characterized by nucleotide sequence analysis. The human BSF-2/IL-6 gene consists of five exons and four...
Ookata, K, Hisanaga, S, Okano, T, Tachibana, K, Kishimoto, T
M phase promoting factor (MPF) is a major element controlling entry into the M phase of the eukaryotic cell cycle. MPF is composed of two subunits, p34cdc2 and cyclin B. Using indirect...
Immunoglobulin gene expression and DNA methylation in murine pre-B cell lines.
Akira, S, Sugiyama, H, Sakaguchi, N, Kishimoto, T
DNA modification accompanying immunoglobulin gene expression was examined in various Abelson murine leukemia virus (A-MuLV)-transformed cell lines, which were able to differentiate from the mu- to...
Schooltink, H, Stoyan, T, Lenz, D, Schmitz, H, Hirano, T, Kishimoto, T, ...
cDNAs coding for the human hepatic interleukin-6 receptor (IL-6-R) have been isolated from a library made from poly(A) RNA of dexamethasone-treated human hepatoma cells (HepG2). We found the hepatic...
Kasai, H, Takagi, H, Ninomiya, Y, Kishimoto, T, Ito, K, Yoshida, A, ...
1. Changes in membrane capacitance evoked by the rapid photolysis of a caged Ca2+ compound, DM-nitrophen or nitrophenyl-EGTA, were investigated in undifferentiated PC12 cells. They were interpreted...
Kinetic diversity in the fusion of exocytotic vesicles.
Ninomiya, Y, Kishimoto, T, Yamazawa, T, Ikeda, H, Miyashita, Y, Kasai, H
The speed at which secretory vesicles fuse with the plasma membrane is a key parameter for neuronal and endocrine functions. We determined the precise time courses for fusion of small clear and large...
Tachibana, K, Machida, T, Nomura, Y, Kishimoto, T
The mechanism by which fertilization initiates S-phase in the zygote is examined by manipulating the activity of MAP kinase in mature starfish eggs. These unfertilized eggs, which are arrested at...
Defective adipocyte differentiation in mice lacking the C/EBPbeta and/or C/EBPdelta gene.
Tanaka, T, Yoshida, N, Kishimoto, T, Akira, S
To investigate the role of C/EBP family members during adipocyte differentiation in vivo, we have generated mice lacking the C/EBPbeta and/or C/EBPdelta by gene targeting. Approximately 85% of...
Kamiya, A, Kinoshita, T, Ito, Y, Matsui, T, Morikawa, Y, Senba, E, ...
Fetal liver, the major site of hematopoiesis during embryonic development, acquires additional various metabolic functions near birth. Although liver development has been characterized biologically...
Interleukin 6 activates heat-shock protein 90 beta gene expression.
Stephanou, A, Amin, V, Isenberg, D A, Akira, S, Kishimoto, T, Latchman, D S
The levels of the cytokine interleukin-6 (IL-6) and the heat-shock protein hsp90 have both been reported to be elevated in patients with active systemic lupus erythematosus (SLE). We show that hsp90...
Stephanou, A, Isenberg, D A, Akira, S, Kishimoto, T, Latchman, D S
The levels of the 90 kDa heat-shock protein (hsp90) and the activity of the hsp90beta gene promoter are increased in response to treatment by interleukin (IL)-6. The hsp90beta gene promoter contains...
New Criteria for Immunofluorescence Assay for Q Fever Diagnosis in Japan
Setiyono, A., Ogawa, M., Cai, Y., Shiga, S., Kishimoto, T., Kurane, I.
A study was made to evaluate the cutoff value of indirect immunofluorescent-antibody (IFA) test for Q fever diagnosis in Japan. We used 346 sera, including 16 from confirmed Q fever cases, 304 from...
Quantitative analysis of integrated Ed alpha gene expression in C57BL/6 transgenic mice.
Kashiwamura, S I, Sadakane, Y, Kikutani, H, Kishimoto, T, Kimoto, M
We performed quantitative analysis of Ed alpha gene expression in the transgenic mice, created by microinjecting cloned Ed alpha gene fragments into C57BL/6 fertilized eggs. DNA dot-blot analysis...
Anti-interleukin 6 receptor antibody treatment in rheumatic disease
Nishimoto, N., Kishimoto, T., Yoshizaki, K.
Interleukin 6 (IL6) is a pleiotropic cytokine with a wide range of biological activities. IL6 transgene into mice gives rise to the abnormalities such as hyper-γ-globulinaemia, thrombocytosis,...
Yamauchi-Takihara, K., Nakajima-Taniguchi, C., Matsui, H., Fujio, Y., Kunisada, K., Nagata, S., ...
OBJECTIVE: The disease-bearing genes for hypertrophic cardiomyopathy (HCM) in HCM families have been identified as the beta-myosin heavy chain, alpha-tropomyosin, and cardiac troponin T genes. Three...
Thymus lymphocytes in uraemic rats and the effect of thymosin fraction 5 in vitro.
Ikemoto, S, Kishimoto, T, Wada, S, Hayahara, N, Maekawa, M
Cellular immunity of thymus lymphocytes in uraemic rats was studied. Severe and moderate uraemia was induced in rats, and sham-operated and normal rats were used as the controls. As a result, the...
Kishimoto, T, Ralph, P, Good, R A
The effect of anti-immunoglobulin (anti-Ig) on cultures of established human B-cell lines was studied to develop models for the initiation and regulation of immune responses. DNA synthesis in one...
The role of basophils and mast cells in cutaneous basophil hypersensitivity reaction.
Kishimoto, T, Soda, R, Takahashi, K, Kimura, I
Basophils are the main cell component in cutaneous basophil hypersensitivity (CBH) reactions, but the role of basophils and the factors which gather them into CBH reaction sites are unknown. To...
Thymus lymphocytes in uraemic rats and the effect of thymosin fraction 5 in vivo.
Ikemoto, S, Kamizuru, M, Hayahara, N, Okamoto, S, Wada, S, Kishimoto, T, ...
Thymus lymphocyte subsets in uraemic rats were studied using monoclonal antibodies. Severe and moderate uraemia was induced in rats, and sham-operated and normal rats were used as the controls. As a...
Serum levels of soluble Fas ligand in patients with silicosis
Tomokuni, A, Otsuki, T, Isozaki, Y, Kita, S, Ueki, H, Kusaka, M, ...
Certain patients with silicosis have been reported to exhibit immunological abnormalities such as the appearance of antinuclear antibodies and the occurrence of autoimmune diseases. Fas ligand (FasL)...
Karaki, H., Kishimoto, T., Ozaki, H., Sakata, K., Umeno, H., Urakawa, N.
Effects of harmaline and other harmala alkaloids on the contractions induced in the vascular smooth muscle of rabbit aorta and intestinal smooth muscle of taenia isolated from guinea-pig caecum were...
TOMIOKA, H, KISHIMOTO, T, MAW, W W
We studied the role of phospholipids and nitric oxide in expression of the suppressor activity of splenic macrophages induced by Mycobacterium avium-intracellulare complex infection (MAIC-induced...
TOMOKUNI, A, AIKOH, T, MATSUKI, T, ISOZAKI, Y, OTSUKI, T, KITA, S, ...
Soluble Fas (sFas) is produced as translation products of alternative mRNA splicing, and antagonizes the membranous Fas molecule in Fas/Fas ligand interactions. We investigated the serum sFas levels...
Aono, A, Enomoto, H, Yoshida, N, Yoshizaki, K, Kishimoto, T, Komori, T
The repertoire of lymphocyte receptor genes encoded in a germline is further diversified by a number of processes, including the template-independent addition of nucleotides (N regions) by means of...
Mass Dependence of Lifetimes of hypernuclei
H. Bhang, H. Park, Y. D. Kim, M. Youn, O. Hashimoto, K. Maeda, ...
We have determined the lifetimes of hypernuclei over a broad mass range, A=1156, from which its characteristic mass dependence is clearly observed. The lifetime decreases slightly from that of free...
Adolescent smoking behaviour and cigarette brand preference in Japan
Osaki, Y, Tanihata, T, Ohida, T, Minowa, M, Wada, K, Suzuki, K, ...
Interleukin-6, tumour necrosis factor α and interleukin-1β in patients with renal cell carcinoma
Yoshida, N, Ikemoto, S, Narita, K, Sugimura, K, Wada, S, Yasumoto, R, ...
As regulators of malignant cell behaviour and communication with stroma, cytokines have proved useful in understanding cancer biology and developing novel therapies. In renal cell carcinoma, patients...