Deciphering the diversity of small RNAs in plants: the long and short of it (2009)
Schwach, Frank, Moxon, Simon, Moulton, Vincent, Dalmay, Tamas
RNA silencing is a complex and highly conserved regulatory mechanism that is now known to be involved in such diverse processes as development, pathogen control, genome maintenance and response to...
Szittya, Gyorgy, Moxon, Simon, Santos, Dulce M, Jing, Runchun, Fevereiro, Manuel PS, Moulton, Vincent, ...
Abstract Background High-throughput sequencing technology is capable to identify novel short RNAs in plant species. We used Solexa sequencing to find new microRNAs in one of the model legume species,...
A toolkit for analysing large-scale plant small RNA datasets (2008)
Moxon, Simon, Schwach, Frank, Dalmay, Tamas, MacLean, Dan, Studholme, David J., Moulton, Vincent
Summary: Recent developments in high-throughput sequencing technologies have generated considerable demand for tools to analyse large datasets of small RNA sequences. Here, we describe a suite of...
Experimental identification of microRNA-140 targets by silencing and overexpressing miR-140 (2008)
Nicolas, Francisco Esteban, Pais, Helio, Schwach, Frank, Lindow, Morten, Kauppinen, Sakari, Moulton, Vincent, ...
MicroRNAs (miRNAs) are short noncoding RNA molecules regulating the expression of mRNAs. Target identification of miRNAs is computationally difficult due to the relatively low homology between miRNAs...
SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis
Dalmay, Tamas, Horsefield, Rob, Braunstein, Thomas Hartig, Baulcombe, David C.
Post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes. Arabidopsis plants carrying mutations at the SDE3 locus are...
Potato Virus X Amplicons in Arabidopsis Mediate Genetic and Epigenetic Gene Silencing
Dalmay, Tamas, Hamilton, Andrew, Mueller, Elisabeth, Baulcombe, David C.
Amplicon transgenes from potato virus X (PVX) are based on a modified version of the viral genome and are efficient activators of post-transcriptional gene silencing (PTGS). To determine whether PVX...
SDE3 encodes an RNA helicase required for post-transcriptional gene silencing in Arabidopsis
Dalmay, Tamas, Horsefield, Rob, Braunstein, Thomas Hartig, Baulcombe, David C.
Post-transcriptional gene silencing (PTGS) provides protection in plants against virus infection and can suppress expression of transgenes. Arabidopsis plants carrying mutations at the SDE3 locus are...
Potato Virus X Amplicons in Arabidopsis Mediate Genetic and Epigenetic Gene Silencing
Dalmay, Tamas, Hamilton, Andrew, Mueller, Elisabeth, Baulcombe, David C.
Amplicon transgenes from potato virus X (PVX) are based on a modified version of the viral genome and are efficient activators of post-transcriptional gene silencing (PTGS). To determine whether PVX...
Short RNAs in environmental adaptation
Non-coding small RNAs (19–24 nucleotide long) have recently been recognized as the important regulator of gene expression in both plants and animals. Several classes of endogenous short RNAs have...
High-throughput sequencing of Medicago truncatula short RNAs identifies eight new miRNA families
Szittya, Gyorgy, Moxon, Simon, Santos, Dulce M, Jing, Runchun, Fevereiro, Manuel PS, Moulton, Vincent, ...
Deep sequencing of tomato short RNAs identifies microRNAs targeting genes involved in fruit ripening
Moxon, Simon, Jing, Runchun, Szittya, Gyorgy, Schwach, Frank, Rusholme Pilcher, Rachel L., Moulton, Vincent, ...
In plants there are several classes of 21–24-nt short RNAs that regulate gene expression. The most conserved class is the microRNAs (miRNAs), although some miRNAs are found only in specific...
Experimental identification of microRNA-140 targets by silencing and overexpressing miR-140
Nicolas, Francisco Esteban, Pais, Helio, Schwach, Frank, Lindow, Morten, Kauppinen, Sakari, Moulton, Vincent, ...
MicroRNAs (miRNAs) are short noncoding RNA molecules regulating the expression of mRNAs. Target identification of miRNAs is computationally difficult due to the relatively low homology between miRNAs...