Qian Wang, Hisashi Fujioka, Victor Nussenzweig
Plasmodium sporozoites develop within oocysts residing in the mosquito midgut. Mature sporozoites exit the oocysts, enter the hemolymph, and invade the salivary glands. The circumsporozoite (CS)...
Wang, Qian, Fujioka, Hisashi, Nussenzweig, Victor, Haldar, Kasturi
Plasmodium sporozoites develop within oocysts residing in the mosquito midgut. Mature sporozoites exit the oocysts, enter the hemolymph, and invade the salivary glands. The circumsporozoite (CS)...
Levels of circumsporozoite protein in the Plasmodium oocyst determine sporozoite morphology. (2002)
Thathy, Vandana, Fujioka, Hisashi, Gantt, Soren, Nussenzweig, Ruth, Nussenzweig, Victor, M Nard, Robert
The sporozoite stage of the Plasmodium parasite is formed by budding from a multinucleate oocyst in the mosquito midgut. During their life, sporozoites must infect the salivary glands of the mosquito...
RENATO A. MORTARA, FILIP VANDEKERCKHOVE, VICTOR NUSSENZWEIG
Trypanosoma cruzi trypomastigotes treated with phosphatidylinositol-specific phospholipase C (PI-PLC) in vitro are rapidly induced to differentiate into round forms. Using confocal microscopy, we...
Conservation of a gliding motility and cell invasion machinery in Apicomplexan parasites. (1999)
Kappe, Stefan, Bruderer, Thomas, Gantt, Soren, Fujioka, Hisashi, Nussenzweig, Victor, M Nard, Robert
Most Apicomplexan parasites, including the human pathogens Plasmodium, Toxoplasma, and Cryptosporidium, actively invade host cells and display gliding motility, both actions powered by parasite...
Substrate specificity of the Trypanosoma cruzi trans-sialidase (1992)
Vandekerckhove, Filip, Schenkman, Sergio, Carvalho, Lain Pontes De, Tomlinson, Stephen, Kiso, Makoto, Yoshida, Masahiro, ...
Trypanosoma cruzi trypomastigotes acquire sialic acid (SA) from host glycoconjugates by means of a plasma membrane-associated trans-sialidase (TS). Here we study the substrate specificity of TS,...
Tomlinson, Stephen, Carvalho, Lain Pontes De, Vandekerckhove, Filip, Nussenzweig, Victor
Trypanosoma cruzi trans-sialidase (TS) is a recently described enzyme which transfers α(2–3)-linked sialic acid from host-derived sialylated glycoconjugates to parasite surface molecules...
A novel membrane glycoprotein capable of inhibiting membrane attack by homologous complement (1989)
Okada, Noriko, Harada, Ryoko, Fujita, Teizo, Okada, Hidechika, Nussenzweig, Victor
Neuraminidase-treated human erythrocytes become sensitive to haemolysis by heterologous serum via activation of the alternative complement pathway (ACP), while remaining insensitive to homologous...
Exploring the transcriptome of the malaria sporozoite stage
Kappe, Stefan H. I., Gardner, Malcolm J., Brown, Stuart M., Ross, Jessica, Matuschewski, Kai, Ribeiro, Jose M., ...
Most studies of gene expression in Plasmodium have been concerned with asexual and/or sexual erythrocytic stages. Identification and cloning of genes expressed in the preerythrocytic stages lag far...
Characterization of a Novel Trypanosome Lytic Factor from Human Serum
Raper, Jayne, Fung, Ramie, Ghiso, Jorge, Nussenzweig, Victor, Tomlinson, Stephen
Natural resistance of humans to the cattle pathogen Trypanosoma brucei brucei has been attributed to the presence in human serum of nonimmune factors that lyse the parasite. Normal human serum...
Gantt, Soren, Persson, Cathrine, Rose, Keith, Birkett, Ashley J., Abagyan, Ruben, Nussenzweig, Victor
Thrombospondin-related anonymous protein (TRAP), a candidate malaria vaccine antigen, is required for Plasmodium sporozoite gliding motility and cell invasion. For the first time, the ability of...
Proteasome Inhibitors Block Development of Plasmodium spp.
Gantt, Soren M., Myung, Joon Mo, Briones, Marcelo R. S., Li, Wei Dong, Corey, E. J., Omura, Satoshi, ...
Proteasomes degrade most of the proteins inside eukaryotic cells, including transcription factors and regulators of cell cycle progression. Here we show that nanomolar concentrations of lactacystin,...
Green Fluorescent Protein as a Marker in Plasmodium berghei Transformation
Sultan, Ali A., Thathy, Vandana, Nussenzweig, Victor, Ménard, Robert
We present a new marker that confers both resistance to pyrimethamine and green fluorescent protein-based fluorescence on the malarial parasite Plasmodium berghei. A single copy of the cassette...
Matuschewski, Kai, Nunes, Alvaro C., Nussenzweig, Victor, Ménard, Robert
Plasmodium sporozoites, the transmission form of the malaria parasite, successively invade salivary glands in the mosquito vector and the liver in the mammalian host. Sporozoite capacity to invade...
Levels of circumsporozoite protein in the Plasmodium oocyst determine sporozoite morphology
Thathy, Vandana, Fujioka, Hisashi, Gantt, Soren, Nussenzweig, Ruth, Nussenzweig, Victor, Ménard, Robert
The sporozoite stage of the Plasmodium parasite is formed by budding from a multinucleate oocyst in the mosquito midgut. During their life, sporozoites must infect the salivary glands of the mosquito...
Dukor, Peter, Bianco, Celso, Nussenzweig, Victor
To determine the tissue localization of lymphocytes provisionally termed „complement-receptor lymphocytes,” which are characterized by having a membrane receptor for antigen-antibody-complement...
Sites of Interaction between Aldolase and Thrombospondin-related Anonymous Protein in Plasmodium
Buscaglia, Carlos A., Coppens, Isabelle, Hol, Wim G. J., Nussenzweig, Victor
Gliding motility and host cell invasion by apicomplexan parasites are empowered by an acto-myosin motor located underneath the parasite plasma membrane. The motor is connected to host cell receptors...
Human Lymphocytes Bear Membrane Receptors for C3b and C3d
Eden, Aline, Miller, Gary W., Nussenzweig, Victor
Human peripheral blood lymphocytes have membrane receptors for EAC43b (sheep erythrocytes sensitized with antibody and complement) and also for EAC43d, obtained by treating EAC43b with C3b...
Pappas, Michael G., Nussenzweig, Ruth S., Nussenzweig, Victor, Shear, Hannah Lustig
We investigated the ability of malaria-infected and normal mice to clear particulate immune complexes consisting of autologous erythrocytes sensitized with either IgG or complement.
Wang, Qian, Fujioka, Hisashi, Nussenzweig, Victor
Plasmodium sporozoites develop within oocysts residing in the mosquito midgut. Mature sporozoites exit the oocysts, enter the hemolymph, and invade the salivary glands. The circumsporozoite (CS)...
Bosch, Jürgen, Buscaglia, Carlos A., Krumm, Brian, Ingason, Bjarni P., Lucas, Robert, Roach, Claudia, ...
An actomyosin motor located underneath the plasma membrane drives motility and host-cell invasion of apicomplexan parasites such as Plasmodium falciparum and Plasmodium vivax, the causative agents of...
Evidence-Based Annotation of the Malaria Parasite's Genome Using Comparative Expression Profiling
Zhou, Yingyao, Ramachandran, Vandana, Kumar, Kota Arun, Westenberger, Scott, Refour, Phillippe, Zhou, Bin, ...
A fundamental problem in systems biology and whole genome sequence analysis is how to infer functions for the many uncharacterized proteins that are identified, whether they are conserved across...
Tao, Deng, Barba-Spaeth, Giovanna, Rai, Urvashi, Nussenzweig, Victor, Rice, Charles M., Nussenzweig, Ruth S.
The yellow fever vaccine 17D (17D) is safe, and after a single immunizing dose, elicits long-lasting, perhaps lifelong protective immunity. One of the major challenges facing delivery of human...
DIFFERENCES IN THE ELECTROPHORETIC MOBILITIES OF GUINEA PIG 7S ANTIBODIES OF DIFFERENT SPECIFICITIES
Nussenzweig, Victor, Benacerraf, Baruj
The electrophoretic mobilities of guinea pig γ1 and γ2 antibodies bearing different specificities were compared in agar gel at pH 8.2. Specifically purified antibodies bearing the same...
QUANTITATIVE VARIATIONS IN L CHAIN TYPES IN GUINEA PIG ANTIHAPTEN ANTIBODIES
Nussenzweig, Victor, Benacerraf, Baruj
In guinea pig purified antihapten antibodies, the proportion of molecules bearing the κ- or λ-type of L chains (K or L molecules) may diverge markedly from that found in normal γ2-globulins. This...
PRESENCE OF TWO TYPES OF L POLYPEPTIDE CHAINS IN GUINEA PIG 7S IMMUNOGLOBULINS
Nussenzweig, Victor, Lamm, Michael E., Benacerraf, Baruj
Guinea pig 7S immunoglobulins (γ1 and γ2) consist of two groups of molecules (K and L) bearing different types of L chains (κ and λ). Approximately one-third of the molecules in normal guinea pig...
Green, Ira, Vassalli, Pierre, Nussenzweig, Victor, Benacerraf, Baruj
A combination of double immunofluorescent technique and radioautographic localization of radioactive antigens was used to investigate the question whether single antibody-producing cells can make...
ANTIHAPTEN ANTIBODY SPECIFICITY AND L CHAIN TYPE
Nussenzweig, Victor, Benacerraf, Baruj
Two types of light polypeptide chain (κ and λ) are present in guinea pig immunoglobulins. The ratios of K/L molecules in anti-hapten antibodies differ sometimes markedly from that found in normal...
RECEPTORS FOR COMPLEMENT ON LEUKOCYTES
Lay, Waltraut H., Nussenzweig, Victor
Sheep red blood cells sensitized by 7S, but not by 19S rabbit anti-Forssman antibodies, adhere and form rosettes on mouse macrophages and on a few monocytes and polymorphonuclear cells (PMN). When,...
Bianco, Celso, Patrick, Richard, Nussenzweig, Victor
A population of lymphoid cells from several animal species, including man, was identified through a membrane receptor which binds sheep red blood cells treated with antibody and complement. When...
Pincus, Carolyn S., Lamm, Michael E., Nussenzweig, Victor
The ability of passively administered antibody to suppress the immune response against homologous antigenic determinants while concomitantly enhancing the response against other unrelated...
Vitetta, Ellen S., Bianco, Celso, Nussenzweig, Victor, Uhr, Jonathan W.
Thymocytes, bone marrow cells, and their derived T and B cell populations were examined for the presence of Ig by the cell surface radioiodination technique. Both IgM and IgG were identified on bone...
COMPLEMENT-DEPENDENT RELEASE OF IMMUNE COMPLEXES FROM THE LYMPHOCYTE MEMBRANE
Miller, Gary W., Saluk, Paul H., Nussenzweig, Victor
Soluble antigen-antibody-complement complexes bound to mouse B lymphocytes are rapidly released from the cell membrane in the presence of normal serum from several mammalian species. The release is...
Mapping the Active Site of CD59
Yu, Jinghua, Abagyan, Ruben, Dong, Shanghong, Gilbert, Alexander, Nussenzweig, Victor, Tomlinson, Stephen
CD59 is a widely distributed membrane-bound inhibitor of the cytolytic membrane attack complex (MAC) of complement. This small (77 amino acid) glycoprotein is a member of the Ly6 superfamily of...
Conservation of a Gliding Motility and Cell Invasion Machinery in Apicomplexan Parasites
Kappe, Stefan, Bruderer, Thomas, Gantt, Soren, Fujioka, Hisashi, Nussenzweig, Victor, Ménard, Robert
Most Apicomplexan parasites, including the human pathogens Plasmodium, Toxoplasma, and Cryptosporidium, actively invade host cells and display gliding motility, both actions powered by parasite...
Class II-Restricted Protective Immunity Induced by Malaria Sporozoites▿
Oliveira, Giane A., Kumar, Kota Arun, Calvo-Calle, J. Mauricio, Othoro, Caroline, Altszuler, David, Nussenzweig, Victor, ...
The irradiated-sporozoite vaccine elicits sterile immunity against Plasmodium parasites in experimental rodent hosts and human volunteers. Based on rodent malaria models, it has been proposed that...
Kumar, Kota Arun, Baxter, Peter, Tarun, Alice S., Kappe, Stefan H. I., Nussenzweig, Victor
Immunization with radiation attenuated Plasmodium sporozoites (RAS) elicits sterile protective immunity against sporozoite challenge in murine models and in humans. Similarly to RAS, the genetically...