In vivo gene expression: DNA electrotransfer (2003)
Trezise, A. E., Palazon, L., Davies, W. L., Colledge, W. H.
The use of electric pulses to deliver therapeutic molecules to tissues and organs in vivo is a rapidly growing field of research. Electrotransfer can be used to deliver a wide range of potentially...
In vivo gene expression: DNA electrotransfer (2003)
Trezise, A. E., Palazon, L., Davies, W. L., Colledge, W. H.
The use of electric pulses to deliver therapeutic molecules to tissues and organs in vivo is a rapidly growing field of research. Electrotransfer can be used to deliver a wide range of potentially...
Mucus altering agents as adjuncts for nonviral gene transfer to airway epithelium (2001)
Ferrari, S., Kitson, C., Farley, R., Steel, R., Marriott, C., Parkins, D. A., ...
Nonviral vectors have been shown to be a safe and valid alternative to recombinant viruses for gene therapy of cystic fibrosis (CF). Nevertheless, gene transfer efficiency needs to be increased...
Mucus altering agents as adjuncts for nonviral gene transfer to airway epithelium (2001)
Ferrari, S., Kitson, C., Farley, R., Steel, R., Marriott, C., Parkins, D. A., ...
Nonviral vectors have been shown to be a safe and valid alternative to recombinant viruses for gene therapy of cystic fibrosis (CF). Nevertheless, gene transfer efficiency needs to be increased...
Cystic fibrosis gene therapy (1995)
Cystic fibrosis is a common severe autosomal recessive genetic disease which is caused by dysfunction of an epithelial cell surface cAMP activated CI– channel. The effects of this dysfunction are...
Impaired cell volume regulation in intestinal crypt epithelia of cystic fibrosis mice.
Valverde, M A, O'Brien, J A, Sepúlveda, F V, Ratcliff, R A, Evans, M J, Colledge, W H
Cystic fibrosis is a disease characterized by abnormalities in the epithelia of the lungs, intestine, salivary and sweat glands, liver, and reproductive systems, often as a result of inadequate...
Markland, W, Oostra, B A, Harvey, R, Markham, A F, Colledge, W H, Smith, A E
Tyrosine residues of middle-T and tyrosine phosphorylation are thought to be important in the transformation of cultured rodent cells by polyomavirus. Of the potential tyrosine sites in the...
Tse, E., Smith, A. J. H., Hunt, S., Lavenir, I., Forster, A., Warren, A. J., ...
The LIM-only family of proteins comprises four members; two of these (LMO1 and LMO2) are involved in human T-cell leukemia via chromosomal translocations, and LMO2 is a master regulator of...
Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen.
Colledge, W H, Richardson, W D, Edge, M D, Smith, A E
Site-directed mutagenesis was used to change Lys-128 of the simian virus 40 large-T nuclear location signal to Met, Ile, Arg, Gln, Asn, Leu, or His. Except for the large-T antigen of the Arg...
Challis, B. G., Coll, A. P., Yeo, G. S. H., Pinnock, S. B., Dickson, S. L., Thresher, R. R., ...
Inactivating mutations of the pro-opiomelanocortin (POMC) gene in both mice and humans leads to hyperphagia and obesity. To further examine the mechanisms whereby POMC-deficiency leads to disordered...
Complementation of null CF mice with a human CFTR YAC transgene.
Manson, A L, Trezise, A E, MacVinish, L J, Kasschau, K D, Birchall, N, Episkopou, V, ...
We have made transgenic mice carrying a 320 kb YAC with the intact human cystic fibrosis transmembrane regulator (CFTR) gene. Mice that only express the human transgene were obtained by breeding with...
Gilmour, D T, Lyon, G J, Carlton, M B, Sanes, J R, Cunningham, J M, Anderson, J R, ...
SPARC (secreted protein acidic and rich in cysteine, also known as osteonectin/BM40) is a secreted Ca2+-binding glycoprotein that interacts with a range of extracellular matrix molecules, including...
The genetic advantage hypothesis in cystic fibrosis heterozygotes: a murine study.
Cuthbert, A W, Halstead, J, Ratcliff, R, Colledge, W H, Evans, M J
1. The delta F508 mutation of the cystic fibrosis (CF) gene is of high frequency in man (1 in 25) and in homozygotes causes cystic fibrosis. It is suggested that cystic fibrosis heterozygotes...
MacVinish, L J, Gill, D R, Hyde, S C, Mofford, K A, Evans, M J, Higgins, C F, ...
1. An improved novel plasmid backbone, pTrial10, has been developed. We have used this vector to deliver the cDNA for the cystic fibrosis transmembrane conductance regulator (CFTR) to cells, both in...
Seidler, U, Blumenstein, I, Kretz, A, Viellard-Baron, D, Rossmann, H, Colledge, W H, ...
1. Most segments of the gastrointestinal tract secrete HCO3-, but the molecular nature of the secretory mechanisms has not been identified. We had previously speculated that the regulator for...
Impaired cell volume regulation in intestinal crypt epithelia of cystic fibrosis mice.
Valverde, M A, O'Brien, J A, Sepúlveda, F V, Ratcliff, R A, Evans, M J, Colledge, W H
Cystic fibrosis is a disease characterized by abnormalities in the epithelia of the lungs, intestine, salivary and sweat glands, liver, and reproductive systems, often as a result of inadequate...
Markland, W, Oostra, B A, Harvey, R, Markham, A F, Colledge, W H, Smith, A E
Tyrosine residues of middle-T and tyrosine phosphorylation are thought to be important in the transformation of cultured rodent cells by polyomavirus. Of the potential tyrosine sites in the...
Tse, E., Smith, A. J. H., Hunt, S., Lavenir, I., Forster, A., Warren, A. J., ...
The LIM-only family of proteins comprises four members; two of these (LMO1 and LMO2) are involved in human T-cell leukemia via chromosomal translocations, and LMO2 is a master regulator of...
Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen.
Colledge, W H, Richardson, W D, Edge, M D, Smith, A E
Site-directed mutagenesis was used to change Lys-128 of the simian virus 40 large-T nuclear location signal to Met, Ile, Arg, Gln, Asn, Leu, or His. Except for the large-T antigen of the Arg...
Challis, B. G., Coll, A. P., Yeo, G. S. H., Pinnock, S. B., Dickson, S. L., Thresher, R. R., ...
Inactivating mutations of the pro-opiomelanocortin (POMC) gene in both mice and humans leads to hyperphagia and obesity. To further examine the mechanisms whereby POMC-deficiency leads to disordered...
The genetic advantage hypothesis in cystic fibrosis heterozygotes: a murine study.
Cuthbert, A W, Halstead, J, Ratcliff, R, Colledge, W H, Evans, M J
1. The delta F508 mutation of the cystic fibrosis (CF) gene is of high frequency in man (1 in 25) and in homozygotes causes cystic fibrosis. It is suggested that cystic fibrosis heterozygotes...
MacVinish, L J, Gill, D R, Hyde, S C, Mofford, K A, Evans, M J, Higgins, C F, ...
1. An improved novel plasmid backbone, pTrial10, has been developed. We have used this vector to deliver the cDNA for the cystic fibrosis transmembrane conductance regulator (CFTR) to cells, both in...
Seidler, U, Blumenstein, I, Kretz, A, Viellard-Baron, D, Rossmann, H, Colledge, W H, ...
1. Most segments of the gastrointestinal tract secrete HCO3-, but the molecular nature of the secretory mechanisms has not been identified. We had previously speculated that the regulator for...
Complementation of null CF mice with a human CFTR YAC transgene.
Manson, A L, Trezise, A E, MacVinish, L J, Kasschau, K D, Birchall, N, Episkopou, V, ...
We have made transgenic mice carrying a 320 kb YAC with the intact human cystic fibrosis transmembrane regulator (CFTR) gene. Mice that only express the human transgene were obtained by breeding with...
Gilmour, D T, Lyon, G J, Carlton, M B, Sanes, J R, Cunningham, J M, Anderson, J R, ...
SPARC (secreted protein acidic and rich in cysteine, also known as osteonectin/BM40) is a secreted Ca2+-binding glycoprotein that interacts with a range of extracellular matrix molecules, including...
Arrhythmogenic mechanisms in the isolated perfused hypokalaemic murine heart
Killeen, M J, Thomas, G, Gurung, I S, Goddard, C A, Fraser, J A, Mahaut-Smith, M P, ...
Joseph, S S, Lynham, J A, Grace, A A, Colledge, W H, Kaumann, A J
Substitution of arginine by glycine at position 389, a frequent β1-adrenoceptor polymorphism, reduces adenylyl cyclase stimulation by (−)-isoprenaline. β1-Adrenoceptors mediate the effects of...
Cuthbert, A. W., Hickman, M. E., MacVinish, L. J., Evans, M. J., Colledge, W. H., Ratcliff, R., ...
1. Guanylin, a 15 amino acid endogenous gut peptide, increased the short circuit current (SCC) in the epithelium of the mouse colon, but only when applied to the apical and not the basolateral...
Kibble, J D, Neal, A M, Colledge, W H, Green, R, Taylor, C J
The aims of this study were to investigate (a) if renal Na+ handling was normal in Cftrtm2camΔF508 cystic fibrosis mice, (b) whether adaptation to dietary salt depletion was preserved and (c)...
Bachmann, O, Wüchner, K, Rossmann, H, Leipziger, J, Osikowska, B, Colledge, W H, ...
Defective regulation and/or reduced expression of the Na+-K+-2Cl− cotransporter NKCC1 may contribute to the severe secretory defect that is observed in cystic fibrosis, but data concerning the...
Mutation analysis of the c-mos proto-oncogene in human ovarian teratomas.
De Foy, K. A., Gayther, S. A., Colledge, W. H., Crockett, S., Scott, I. V., Evans, M. J., ...
Female transgenic mice lacking a functional c-mos proto-oncogene develop ovarian teratomas, indicating that c-mos may behave as a tumour-suppressor gene for this type of tumour. We have analysed the...