Wulf, M., Sørum, M., Nes, A. Van, Skov, R., Melchers, W.J., Klaassen, C.H., ...
Pig farmers and veterinarians in contact with livestock in The Netherlands have a higher risk of methicillin-resistant Staphylococcus aureus (MRSA) carriage than the general population. The objective...
Belkum, A. Van, Melchers, W.J., Ijsseldijk, C., Nohlmans, L., Verbrugh, H., Meis, J.F.
An outbreak caused by amoxicillin-resistant Haemophilus influenzae type b was noted among patients suffering from chronic obstructive pulmonary disease. Since infections were clustered in time and...
Structural requirements of the higher order RNA kissing element in the enteroviral 3'UTR.
Wang, J, Bakkers, J M, Galama, J M, Bruins Slot, H J, Pilipenko, E V, Agol, V I, ...
The origin of replication ( oriR ) involved in the initiation of (-) strand enterovirus RNA synthesis is a quasi-globular multi-domain RNA structure which is maintained by a tertiary kissing...
Van Kuppeveld, F J, Galama, J M, Zoll, J, Melchers, W J
Coxsackie B virus protein 2B contains near its C terminus a hydrophobic domain with an amino acid composition that is characteristic for transmembrane regions. A molecular genetic approach was...
Van Kuppeveld, F J, Galama, J M, Zoll, J, Van Den Hurk, P J, Melchers, W J
Enterovirus protein 2B has been shown to increase plasma membrane permeability. We have identified a conserved putative amphipathic alpha-helix with a narrow hydrophilic face and an arrangement of...
Mengovirus leader is involved in the inhibition of host cell protein synthesis.
Zoll, J, Galama, J M, Van Kuppeveld, F J, Melchers, W J
The presence of a leader peptide in picornaviruses is restricted to the Cardiovirus and Aphthovirus genera. However, the leader peptides of these two genera are structurally and functionally...
Van Kuppeveld, F J, Van Den Hurk, P J, Zoll, J, Galama, J M, Melchers, W J
The enterovirus 2B/2C cleavage site differs from the common cleavage site motif AxxQ/G by the occurrence of either polar residues at the P1' position or large aliphatic residues at the P4 position....
Melchers, W J, Hoenderop, J G, Bruins Slot, H J, Pleij, C W, Pilipenko, E V, Agol, V I, ...
Higher-order RNA structures in the 3' untranslated region (3'UTR) of enteroviruses are thought to play a pivotal role in viral negative-strand RNA synthesis. The structure of the 3'UTR was predicted...
Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.
Van Kuppeveld, F J, Van Der Logt, J T, Angulo, A F, Van Zoest, M J, Quint, W G, Niesters, H G, ...
Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the identification of variable regions with both genus- and species-specific sequences. Species-specific sequences of...
Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.
Van Kuppeveld, F J, Johansson, K E, Galama, J M, Kissing, J, Bölske, G, Van Der Logt, J T, ...
The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using...
Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.
Van Kuppeveld, F J, Van Der Logt, J T, Angulo, A F, Van Zoest, M J, Quint, W G, Niesters, H G, ...
Hoogkamp-Korstanje, J A, Meis, J F, Kissing, J, Melchers, W J
The risk of cross-colonization and subsequent infection by Pseudomonas aeruginosa in holiday camps for cystic fibrosis patients was studied in 91 children by culturing sputum at their arrival, at...
Verweij, P E, Latgé, J P, Rijs, A J, Melchers, W J, De Pauw, B E, Hoogkamp-Korstanje, J A, ...
The performance of a sandwich enzyme-linked immunosorbent assay (ELISA) which detects Aspergillus galactomannan (GM) was evaluated in bronchoalveolar lavage (BAL) fluid samples from 19 patients who...
Verweij, P E, Meis, J F, Sarfati, J, Hoogkamp-Korstanje, J A, Latgé, J P, Melchers, W J
Twenty-three sequential Aspergillus fumigatus sputum isolates, which had been collected over a period of 2 years, from two patients with cystic fibrosis were genotyped by random amplified polymorphic...
Van Belkum, A, Melchers, W J, Ijsseldijk, C, Nohlmans, L, Verbrugh, H, Meis, J F
An outbreak caused by amoxicillin-resistant Haemophilus influenzae type b was noted among patients suffering from chronic obstructive pulmonary disease. Since infections were clustered in time and...
Van Kuppeveld, F J, Melchers, W J, Willemse, H F, Kissing, J, Galama, J M, Van Der Logt, J T
Recently, an rRNA-based polymerase chain reaction (PCR) has been developed for the detection of murine mycoplasmas at both the genus and species level (F. J. M. van Kuppeveld, J. T. M. van der Logt,...
Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.
Tjhie, J H, Van Kuppeveld, F J, Roosendaal, R, Melchers, W J, Gordijn, R, MacLaren, D M, ...
The sensitivities of three methods of detection of Mycoplasma pneumoniae by a 16S rDNA PCR were compared by using a serial dilution of M. pneumoniae. These methods consisted of a PCR performed...
General primer-mediated PCR for detection of Aspergillus species.
Melchers, W J, Verweij, P E, Van Belkum, A, De Pauw, B E, Hoogkamp-Korstanje, J A, ...
A PCR assay was developed for the diagnosis of invasive aspergillosis in immunocompromised patients. For this purpose, the complete nucleotide sequences of the genes encoding the 18S rRNA of...
Enzymatic amplification of lactate dehydrogenase-elevating virus.
Van Der Logt, J T, Kissing, J, Melchers, W J
To improve the detection of lactate dehydrogenase-elevating virus (LDV), we developed a PCR assay. Primers were selected from ORF7, encoding nucleocapsid protein VP1. No specific amplification was...
Zoll, G J, Melchers, W J, Kopecka, H, Jambroes, G, Van Der Poel, H J, Galama, J M
The aim of this study was to determine the applicability of the polymerase chain reaction (PCR) for routine diagnostic use and for the detection of persistent enteroviral infections. To this end,...
Van Belkum, A, Quint, W G, De Pauw, B E, Melchers, W J, Meis, J F
Polymerase chain reaction amplification of repetitive DNA motifs allows discrimination of Aspergillus species. In a preliminary survey, the DNA fingerprints appear to be identical when isolates of...
Melchers, W J, Herbrink, P, Walboomers, J M, Meijer, C J, Vd Drift, H, Lindeman, J, ...
Optimal conditions for the screening of cervical scrapes for human papillomavirus (HPV) were investigated by using filter in situ hybridization. Since integrated and episomal HPV can be found, cell...
Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction.
Melchers, W J, Schift, R, Stolz, E, Lindeman, J, Quint, W G
Human papillomavirus (HPV) detection was performed using the polymerase chain reaction technique on urine samples from 17 male patients with condylomata acuminata in the meatus urethrae. Urine...
Pilipenko, E V, Poperechny, K V, Maslova, S V, Melchers, W J, Slot, H J, Agol, V I
The key steps in the replication of the poliovirus genome, initiation of (-) and (+) strands, require two different cis-acting elements, oriR and oriL, respectively. It has been proposed that the...
Odinot, P T, Meis, J F, Hoogkamp-Korstanje, J A, Melchers, W J
AIM: The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH). METHODS: Y enterocolitica infected cell lines, rat spleens, and...
Autoimmune markers are undetectable in end stage idiopathic dilated cardiomyopathy.
De Leeuw, N, Melchers, W J, Ruiter, D J, Caforio, A L, Balk, A H, De Jonge, N, ...
BACKGROUND: Autoreactive humoral and cellular immune responses may be involved in the pathogenesis of idiopathic dilated cardiomyopathy (IDC). Certain human leucocyte antigens (HLA) could also be...
Van Kuppeveld, F J, Hoenderop, J G, Smeets, R L, Willems, P H, Dijkman, H B, Galama, J M, ...
Digital-imaging microscopy was performed to study the effect of Coxsackie B3 virus infection on the cytosolic free Ca2+ concentration and the Ca2+ content of the endoplasmic reticulum (ER). During...
Melchers, W J, Bakkers, J M, Bruins Slot, H J, Galama, J M, Agol, V I, Pilipenko, E V
The coxsackie B3 virus oriR is an element of viral RNA thought to promote the assembly of a ribonucleoprotein complex involved in the initiation of genome replication. The mutual orientation of its...
Structural requirements of the higher order RNA kissing element in the enteroviral 3'UTR.
Wang, J, Bakkers, J M, Galama, J M, Bruins Slot, H J, Pilipenko, E V, Agol, V I, ...
The origin of replication ( oriR ) involved in the initiation of (-) strand enterovirus RNA synthesis is a quasi-globular multi-domain RNA structure which is maintained by a tertiary kissing...
Van Kuppeveld, F J, Galama, J M, Zoll, J, Melchers, W J
Coxsackie B virus protein 2B contains near its C terminus a hydrophobic domain with an amino acid composition that is characteristic for transmembrane regions. A molecular genetic approach was...
Van Kuppeveld, F J, Galama, J M, Zoll, J, Van Den Hurk, P J, Melchers, W J
Enterovirus protein 2B has been shown to increase plasma membrane permeability. We have identified a conserved putative amphipathic alpha-helix with a narrow hydrophilic face and an arrangement of...
Mengovirus leader is involved in the inhibition of host cell protein synthesis.
Zoll, J, Galama, J M, Van Kuppeveld, F J, Melchers, W J
The presence of a leader peptide in picornaviruses is restricted to the Cardiovirus and Aphthovirus genera. However, the leader peptides of these two genera are structurally and functionally...
Van Kuppeveld, F J, Van Den Hurk, P J, Zoll, J, Galama, J M, Melchers, W J
The enterovirus 2B/2C cleavage site differs from the common cleavage site motif AxxQ/G by the occurrence of either polar residues at the P1' position or large aliphatic residues at the P4 position....
Melchers, W J, Hoenderop, J G, Bruins Slot, H J, Pleij, C W, Pilipenko, E V, Agol, V I, ...
Higher-order RNA structures in the 3' untranslated region (3'UTR) of enteroviruses are thought to play a pivotal role in viral negative-strand RNA synthesis. The structure of the 3'UTR was predicted...
Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.
Van Kuppeveld, F J, Van Der Logt, J T, Angulo, A F, Van Zoest, M J, Quint, W G, Niesters, H G, ...
Systematic computer alignment of mycoplasmal 16S rRNA sequences allowed the identification of variable regions with both genus- and species-specific sequences. Species-specific sequences of...
Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR.
Van Kuppeveld, F J, Johansson, K E, Galama, J M, Kissing, J, Bölske, G, Van Der Logt, J T, ...
The suitability of a 16S rRNA-based mycoplasma group-specific PCR for the detection of mycoplasma contamination in cell cultures was investigated. A total of 104 cell cultures were tested by using...
Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.
Van Kuppeveld, F J, Van Der Logt, J T, Angulo, A F, Van Zoest, M J, Quint, W G, Niesters, H G, ...
Hoogkamp-Korstanje, J A, Meis, J F, Kissing, J, Melchers, W J
The risk of cross-colonization and subsequent infection by Pseudomonas aeruginosa in holiday camps for cystic fibrosis patients was studied in 91 children by culturing sputum at their arrival, at...
Verweij, P E, Latgé, J P, Rijs, A J, Melchers, W J, De Pauw, B E, Hoogkamp-Korstanje, J A, ...
The performance of a sandwich enzyme-linked immunosorbent assay (ELISA) which detects Aspergillus galactomannan (GM) was evaluated in bronchoalveolar lavage (BAL) fluid samples from 19 patients who...
Verweij, P E, Meis, J F, Sarfati, J, Hoogkamp-Korstanje, J A, Latgé, J P, Melchers, W J
Twenty-three sequential Aspergillus fumigatus sputum isolates, which had been collected over a period of 2 years, from two patients with cystic fibrosis were genotyped by random amplified polymorphic...
Van Belkum, A, Melchers, W J, Ijsseldijk, C, Nohlmans, L, Verbrugh, H, Meis, J F
An outbreak caused by amoxicillin-resistant Haemophilus influenzae type b was noted among patients suffering from chronic obstructive pulmonary disease. Since infections were clustered in time and...
Van Kuppeveld, F J, Melchers, W J, Willemse, H F, Kissing, J, Galama, J M, Van Der Logt, J T
Recently, an rRNA-based polymerase chain reaction (PCR) has been developed for the detection of murine mycoplasmas at both the genus and species level (F. J. M. van Kuppeveld, J. T. M. van der Logt,...
Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.
Tjhie, J H, Van Kuppeveld, F J, Roosendaal, R, Melchers, W J, Gordijn, R, MacLaren, D M, ...
The sensitivities of three methods of detection of Mycoplasma pneumoniae by a 16S rDNA PCR were compared by using a serial dilution of M. pneumoniae. These methods consisted of a PCR performed...
General primer-mediated PCR for detection of Aspergillus species.
Melchers, W J, Verweij, P E, Van Belkum, A, De Pauw, B E, Hoogkamp-Korstanje, J A, ...
A PCR assay was developed for the diagnosis of invasive aspergillosis in immunocompromised patients. For this purpose, the complete nucleotide sequences of the genes encoding the 18S rRNA of...
Enzymatic amplification of lactate dehydrogenase-elevating virus.
Van Der Logt, J T, Kissing, J, Melchers, W J
To improve the detection of lactate dehydrogenase-elevating virus (LDV), we developed a PCR assay. Primers were selected from ORF7, encoding nucleocapsid protein VP1. No specific amplification was...
Zoll, G J, Melchers, W J, Kopecka, H, Jambroes, G, Van Der Poel, H J, Galama, J M
The aim of this study was to determine the applicability of the polymerase chain reaction (PCR) for routine diagnostic use and for the detection of persistent enteroviral infections. To this end,...
Van Belkum, A, Quint, W G, De Pauw, B E, Melchers, W J, Meis, J F
Polymerase chain reaction amplification of repetitive DNA motifs allows discrimination of Aspergillus species. In a preliminary survey, the DNA fingerprints appear to be identical when isolates of...
Melchers, W J, Herbrink, P, Walboomers, J M, Meijer, C J, Vd Drift, H, Lindeman, J, ...
Optimal conditions for the screening of cervical scrapes for human papillomavirus (HPV) were investigated by using filter in situ hybridization. Since integrated and episomal HPV can be found, cell...
Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction.
Melchers, W J, Schift, R, Stolz, E, Lindeman, J, Quint, W G
Human papillomavirus (HPV) detection was performed using the polymerase chain reaction technique on urine samples from 17 male patients with condylomata acuminata in the meatus urethrae. Urine...
Pilipenko, E V, Poperechny, K V, Maslova, S V, Melchers, W J, Slot, H J, Agol, V I
The key steps in the replication of the poliovirus genome, initiation of (-) and (+) strands, require two different cis-acting elements, oriR and oriL, respectively. It has been proposed that the...
Odinot, P T, Meis, J F, Hoogkamp-Korstanje, J A, Melchers, W J
AIM: The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH). METHODS: Y enterocolitica infected cell lines, rat spleens, and...
Autoimmune markers are undetectable in end stage idiopathic dilated cardiomyopathy.
De Leeuw, N, Melchers, W J, Ruiter, D J, Caforio, A L, Balk, A H, De Jonge, N, ...
BACKGROUND: Autoreactive humoral and cellular immune responses may be involved in the pathogenesis of idiopathic dilated cardiomyopathy (IDC). Certain human leucocyte antigens (HLA) could also be...
Van Kuppeveld, F J, Hoenderop, J G, Smeets, R L, Willems, P H, Dijkman, H B, Galama, J M, ...
Digital-imaging microscopy was performed to study the effect of Coxsackie B3 virus infection on the cytosolic free Ca2+ concentration and the Ca2+ content of the endoplasmic reticulum (ER). During...
Melchers, W J, Bakkers, J M, Bruins Slot, H J, Galama, J M, Agol, V I, Pilipenko, E V
The coxsackie B3 virus oriR is an element of viral RNA thought to promote the assembly of a ribonucleoprotein complex involved in the initiation of genome replication. The mutual orientation of its...
Jongen, P J, Zoll, G J, Beaumont, M, Melchers, W J, Van De Putte, L B, Galama, J M
OBJECTIVES--A persistent infection of enteroviruses and cardioviruses has been implicated in polymyositis and dermatomyositis, but conventional hybridisation studies of the presence of enterovirus...
Claas, E. C., Melchers, W. J., Van Der Linden, H. C., Lindeman, J., Quint, W. G.
The polymerase chain reaction (PCR) is used for human papillomavirus (HPV) detection in paraffin-embedded tissue. The specificity of the reaction is unaffected by the method of fixation used before...
Walboomers, J. M., Melchers, W. J., Mullink, H., Meijer, C. J., Struyk, A., Quint, W. G., ...
The sensitivity of human papilloma virus type 16 (HPV-16) DNA detection by DNA in situ hybridization using biotinylated probes (bio-DISH) was estimated by performing this technique on snap-frozen...