Two novel conjugative plasmids from Sulfolobus (2006)
Erauso, G., Stedman, K.M., Zillig, W.
Two conjugative plasmids (CPs) were isolated and characterized from the same 'Sulfolobus islandicus' strain, SOG2/4. The plasmids were separated from each other and transferred into Sulfolobus...
Two novel conjugative plasmids from a single strain of Sulfolobus (2006)
Erauso, G., Stedman, K.M., Zillig, W.
Two conjugative plasmids (CPs) were isolated and characterized from the same 'Sulfolobus islandicus' strain, SOG2/4, The plasmids were separated from each other and transferred into Sulfolobus...
Peng,X., Blum,H., She,Q. X., Mallok,S., Brugger,K., Garrett,R. A., ...
The double-stranded DNA genomes of the viruses SIRV1 and SIRV2, which infect the extremely thermophilic archaeon Sulfolobus and belong to the family Rudiviridae, were sequenced. They are linear,...
Peng, X., Blum, H., She, Q. X., Mallok, S., Brugger, K., Garrett, R. A., ...
The double-stranded DNA genomes of the viruses SIRV1 and SIRV2, which infect the extremely thermophilic archaeon Sulfolobus and belong to the family Rudiviridae, were sequenced. They are linear,...
Electron tomography of ice-embedded prokaryotic cells (1998)
Grimm, R., Singh, H., Rachel, R., Typke, D., Zillig, W., Baumeister, W.
Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm...
THE EVOLUTION OF THE TRANSCRIPTION APPARATUS (1985)
Zillig, W., Schnabel, R., Thomm, Michael, Gropp, F., Reiter, W. D.
DNA-Dependent RNA Polymerases of the Archaebacteria (1985)
Zillig, W., Stetter, Karl Otto, Schnabel, R., Thomm, Michael
PHYLOGENY OF THE ARCHAEBACTERIA AND EUKARYOTES: HOMOLOGY OF THE DNA-DEPENDENT RNA POLYMERASES (1983)
Schnabel, R., Huet, J., Thomm, Michael, Zillig, W., Sentenac, A., Stetter, Karl Otto
Schnabel, R., Thomm, Michael, Gerardy-Schahn, R., Zillig, W., Stetter, Karl Otto, Huet, J.
The archaebacterial DNA-dependent RNA polymerases have a complex structure containing eight or more components. Immunochemical analysis shows an extensive homology between the components of the...
Transcription in archaea: similarity to that in eucarya.
Langer, D, Hain, J, Thuriaux, P, Zillig, W
We present homologies between archaeal and eucaryal DNA-dependent RNA polymerase (RNAP) subunits and transcription factors. The sequences of the Sulfolobus acidocaldarius subunits D, E, and N and...
Klenk, H P, Palm, P, Lottspeich, F, Zillig, W
The gene encoding component H of the DNA-dependent RNA polymerase (RNAP, EC 2.7.7.6) of Sulfolobus acidocaldarius has been identified by comparison of the amino acid sequence with the derived amino...
Schleper, C, Kubo, K, Zillig, W
The lemon-shaped "virus-like" particle SSV1 produced by the thermophilic archaeon Sulfolobus shibatae has not previously been observed to infect any host. Using a plaque assay suitable for the...
Hüdepohl, U, Reiter, W D, Zillig, W
We describe a cell-free transcription system for the archaebacterium Sulfolobus sp. B12 that specifically initiates transcription at the 5S rRNA-encoding DNA and the 16S/23S rRNA-encoding DNA...
Reiter, W D, Hüdepohl, U, Zillig, W
By using a recently developed in vitro transcription assay, the 16S/23S rRNA-encoding DNA promoter from the archaebacterium Sulfolobus sp. B12 was dissected by deletion and linker substitution...
Schleper, C, Holz, I, Janekovic, D, Murphy, J, Zillig, W
A plasmid of 45 kb, designated pNOB8, was found in high copy number in a new heterotrophic Sulfolobus isolate, NOB8H2, from Japan. Dissemination of the plasmid occurred in six cultures of nine...
Schleper, C, Puehler, G, Holz, I, Gambacorta, A, Janekovic, D, Santarius, U, ...
Two species belonging to a novel genus of archaea, designated Picrophilus oshimae and Picrophilus torridus, have been isolated from two different solfataric locations in northern Japan. One habitat...
Hyperthermus butylicus, a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides.
Zillig, W, Holz, I, Janekovic, D, Klenk, H P, Imsel, E, Trent, J, ...
The hyperthermophilic peptide-fermenting sulfur archaebacterium Hyperthermus butylicus was isolated from the sea floor of a solfataric habitat with temperatures of up to 112 degrees C on the coast of...
Pühler, G, Leffers, H, Gropp, F, Palm, P, Klenk, H P, Lottspeich, F, ...
Genes for DNA-dependent RNA polymerase components B, A, and C from the archaebacterium Sulfolobus acidocaldarius and for components B", B', A, and C from the archaebacterium Halobacterium halobium...
Reiter, W D, Palm, P, Voos, W, Kaniecki, J, Grampp, B, Schulz, W, ...
In Sulfolobus sp. strain B12, single-copy genes encode the three ribosomal RNAs. The genes for the 16S rRNA and for the 23S rRNA are closely linked but separated from the 5S rRNA gene. Transcription...
Structure specific ds/ss-RNase activity in the extreme halophile Halobacterium salinarium.
A ds/ss-RNA processing activity involved in antisense-RNA mediated gene regulation in the extremely halophilic archaebacterium Halobacterium salinarium was investigated in vivo. H.salinarium cells...
Palm, P, Schleper, C, Arnold-Ammer, I, Holz, I, Meier, T, Lottspeich, F, ...
An improved purification procedure for Thermotoga maritima RNA-polymerase holoenzyme was developed. The enzyme is highly active with poly dAT or T7 phage DNA as template. DNA gyrase was found to be a...
Pühler, G, Lottspeich, F, Zillig, W
The genes for the three large subunits A, B and C, of the DNA-dependent RNA polymerase of the archaebacterium Sulfolobus acidocaldarius DSM 639, were identified and characterized. The three genes...
Organization of rRNA structural genes in the archaebacterium Thermoplasma acidophilum.
In the archaebacterium Thermoplasma acidophilum, each of the structural genes for 5S, 16S and 23S rRNA occur once per genome. In contrast to those of eubacteria and eukaryotes, they appear unlinked....
Although the antibiotic rifampicin inhibits the transcription of poly[d(A-T)] by E.coli RNA polymerase, a series of short oligonucleotides is produced. It is claimed that the overall inhibition of...
Elements of an archaeal promoter defined by mutational analysis.
Hain, J, Reiter, W D, Hüdepohl, U, Zillig, W
The sequence requirements for specific and efficient transcription from the 16S/23S rRNA promoter of Sulfolobus shibatae were analysed by point mutations and by cassette mutations using an in vitro...
Reiter, W D, Palm, P, Zillig, W
The 5'-termini were precisely mapped for five constitutive and one UV-inducible transcript from the Sulfolobus virus-like particle SSV1. The comparison of the DNA sequences around these...
Reiter, W D, Palm, P, Zillig, W
The precise map positions were determined for the 3'-termini of five transcripts of the Sulfolobus virus-like particle SSV1. In all cases analyzed, these 3'-termini mapped immediately downstream of a...
Zillig, W, Fujiki, H, Blum, W, Janeković, D, Schweiger, M, Rahmsdorf, H, ...
After infection with bacteriophage T7 the beta' and to a lesser extent the beta subunits of E. coli DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) are...
Archaebacteria and eukaryotes possess DNA-dependent RNA polymerases of a common type.
Huet, J, Schnabel, R, Sentenac, A, Zillig, W
DNA-dependent RNA polymerases of archaebacteria not only resemble the nuclear RNA polymerases of eukaryotes rather than the eubacterial enzymes in their complex component patterns but also show...
Electron tomography of ice-embedded prokaryotic cells.
Grimm, R, Singh, H, Rachel, R, Typke, D, Zillig, W, Baumeister, W
Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm...
Prangishvili, D, Arnold, H P, Götz, D, Ziese, U, Holz, I, Kristjansson, J K, ...
The unenveloped, stiff-rod-shaped, linear double-stranded DNA viruses SIRV1 and SIRV2 from Icelandic Sulfolobus isolates form a novel virus family, the Rudiviridae. The sizes of the genomes are 32. 3...
Stedman, K M, Schleper, C, Rumpf, E, Zillig, W
Directed open reading frame (ORF) disruption and a serial selection technique in Escherichia coli and the extremely thermophilic archaeon Sulfolobus solfataricus allowed the identification of...
Transcription in archaea: similarity to that in eucarya.
Langer, D, Hain, J, Thuriaux, P, Zillig, W
We present homologies between archaeal and eucaryal DNA-dependent RNA polymerase (RNAP) subunits and transcription factors. The sequences of the Sulfolobus acidocaldarius subunits D, E, and N and...
Klenk, H P, Palm, P, Lottspeich, F, Zillig, W
The gene encoding component H of the DNA-dependent RNA polymerase (RNAP, EC 2.7.7.6) of Sulfolobus acidocaldarius has been identified by comparison of the amino acid sequence with the derived amino...
Schleper, C, Kubo, K, Zillig, W
The lemon-shaped "virus-like" particle SSV1 produced by the thermophilic archaeon Sulfolobus shibatae has not previously been observed to infect any host. Using a plaque assay suitable for the...
Hüdepohl, U, Reiter, W D, Zillig, W
We describe a cell-free transcription system for the archaebacterium Sulfolobus sp. B12 that specifically initiates transcription at the 5S rRNA-encoding DNA and the 16S/23S rRNA-encoding DNA...
Reiter, W D, Hüdepohl, U, Zillig, W
By using a recently developed in vitro transcription assay, the 16S/23S rRNA-encoding DNA promoter from the archaebacterium Sulfolobus sp. B12 was dissected by deletion and linker substitution...
Schleper, C, Holz, I, Janekovic, D, Murphy, J, Zillig, W
A plasmid of 45 kb, designated pNOB8, was found in high copy number in a new heterotrophic Sulfolobus isolate, NOB8H2, from Japan. Dissemination of the plasmid occurred in six cultures of nine...
Schleper, C, Puehler, G, Holz, I, Gambacorta, A, Janekovic, D, Santarius, U, ...
Two species belonging to a novel genus of archaea, designated Picrophilus oshimae and Picrophilus torridus, have been isolated from two different solfataric locations in northern Japan. One habitat...
Hyperthermus butylicus, a hyperthermophilic sulfur-reducing archaebacterium that ferments peptides.
Zillig, W, Holz, I, Janekovic, D, Klenk, H P, Imsel, E, Trent, J, ...
The hyperthermophilic peptide-fermenting sulfur archaebacterium Hyperthermus butylicus was isolated from the sea floor of a solfataric habitat with temperatures of up to 112 degrees C on the coast of...
Pühler, G, Leffers, H, Gropp, F, Palm, P, Klenk, H P, Lottspeich, F, ...
Genes for DNA-dependent RNA polymerase components B, A, and C from the archaebacterium Sulfolobus acidocaldarius and for components B", B', A, and C from the archaebacterium Halobacterium halobium...
Reiter, W D, Palm, P, Voos, W, Kaniecki, J, Grampp, B, Schulz, W, ...
In Sulfolobus sp. strain B12, single-copy genes encode the three ribosomal RNAs. The genes for the 16S rRNA and for the 23S rRNA are closely linked but separated from the 5S rRNA gene. Transcription...
Structure specific ds/ss-RNase activity in the extreme halophile Halobacterium salinarium.
A ds/ss-RNA processing activity involved in antisense-RNA mediated gene regulation in the extremely halophilic archaebacterium Halobacterium salinarium was investigated in vivo. H.salinarium cells...
Palm, P, Schleper, C, Arnold-Ammer, I, Holz, I, Meier, T, Lottspeich, F, ...
An improved purification procedure for Thermotoga maritima RNA-polymerase holoenzyme was developed. The enzyme is highly active with poly dAT or T7 phage DNA as template. DNA gyrase was found to be a...
Pühler, G, Lottspeich, F, Zillig, W
The genes for the three large subunits A, B and C, of the DNA-dependent RNA polymerase of the archaebacterium Sulfolobus acidocaldarius DSM 639, were identified and characterized. The three genes...
Organization of rRNA structural genes in the archaebacterium Thermoplasma acidophilum.
In the archaebacterium Thermoplasma acidophilum, each of the structural genes for 5S, 16S and 23S rRNA occur once per genome. In contrast to those of eubacteria and eukaryotes, they appear unlinked....
Although the antibiotic rifampicin inhibits the transcription of poly[d(A-T)] by E.coli RNA polymerase, a series of short oligonucleotides is produced. It is claimed that the overall inhibition of...
Elements of an archaeal promoter defined by mutational analysis.
Hain, J, Reiter, W D, Hüdepohl, U, Zillig, W
The sequence requirements for specific and efficient transcription from the 16S/23S rRNA promoter of Sulfolobus shibatae were analysed by point mutations and by cassette mutations using an in vitro...
Reiter, W D, Palm, P, Zillig, W
The 5'-termini were precisely mapped for five constitutive and one UV-inducible transcript from the Sulfolobus virus-like particle SSV1. The comparison of the DNA sequences around these...
Reiter, W D, Palm, P, Zillig, W
The precise map positions were determined for the 3'-termini of five transcripts of the Sulfolobus virus-like particle SSV1. In all cases analyzed, these 3'-termini mapped immediately downstream of a...
Zillig, W, Fujiki, H, Blum, W, Janeković, D, Schweiger, M, Rahmsdorf, H, ...
After infection with bacteriophage T7 the beta' and to a lesser extent the beta subunits of E. coli DNA-dependent RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) are...
Archaebacteria and eukaryotes possess DNA-dependent RNA polymerases of a common type.
Huet, J, Schnabel, R, Sentenac, A, Zillig, W
DNA-dependent RNA polymerases of archaebacteria not only resemble the nuclear RNA polymerases of eukaryotes rather than the eubacterial enzymes in their complex component patterns but also show...
Electron tomography of ice-embedded prokaryotic cells.
Grimm, R, Singh, H, Rachel, R, Typke, D, Zillig, W, Baumeister, W
Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm...
Prangishvili, D, Arnold, H P, Götz, D, Ziese, U, Holz, I, Kristjansson, J K, ...
The unenveloped, stiff-rod-shaped, linear double-stranded DNA viruses SIRV1 and SIRV2 from Icelandic Sulfolobus isolates form a novel virus family, the Rudiviridae. The sizes of the genomes are 32. 3...
Stedman, K M, Schleper, C, Rumpf, E, Zillig, W
Directed open reading frame (ORF) disruption and a serial selection technique in Escherichia coli and the extremely thermophilic archaeon Sulfolobus solfataricus allowed the identification of...