Wanderley de Souza

Structural Organization of Trypanosoma Cruzi   (2009)

De Souza, Wanderley

Since the initial description of Trypanosoma cruzi   by Carlos Chagas in 1909, several research groups have used different microscopic techniques to obtain detailed information about the various...

Toxoplasma gondii : further studies on the subpellicular network (2009)

Lemgruber, Leandro, Kloetzel, John A, De Souza, Wanderley, Vommaro, Rossiane C

The association of the pellicle with cytoskeletal elements in Toxoplasma gondii allows this parasite to maintain its mechanical integrity and makes possible its gliding motility and cell invasion....

Brazilian contribution for a better knowledge on the biology of Toxoplasmagondii   (2009)

De Souza, Wanderley, DaMatta, Renato A., Attias, Márcia

Historically, scientists in Brazil has significantly contributed to the biology, cultivation and structural organization of the pathogenic protozoan Toxoplasmagondii   and its interaction with host...

Expression and subcellular localization of kinetoplast-associated proteins in the different developmental stages of Trypanosoma cruzi (2009)

Cavalcanti, Danielle, Shimada, Márcia, Probst, Christian, Souto-Padrón, Thais, De Souza, Wanderley, Goldenberg, Samuel, ...

Abstract Background The kinetoplast DNA (kDNA) of trypanosomatids consists of an unusual arrangement of circular molecules catenated into a single network. The diameter of the isolated kDNA network...

Growth inhibition and ultrastructural alterations induced by Δ24(25)-sterol methyltransferase inhibitors in Candidaspp. isolates, including non-albicansorganisms (2009)

Ishida, Kelly, Rodrigues, Juliany, Ribeiro, Marcos, Vila, Taíssa, De Souza, Wanderley, Urbina, Julio A, ...

Abstract Background Although Candida species are commensal microorganisms, they can cause many invasive fungal infections. In addition, antifungal resistance can contribute to failure of treatment....

Prostaglandin E2-EP1 and EP2 receptor signaling promotes apical junctional complex disassembly of Caco-2 human colorectal cancer cells (2008)

Tanaka, Marcelo N, Diaz, Bruno L, De Souza, Wanderley, Morgado-Diaz, Jose A

Abstract Background The apical junctional complex (AJC) is a dynamic structure responsible to maintain epithelial cell-cell adhesions and it plays important functions such as, polarity, mechanical...

Electron microscopy of trypanosomes - A historical view (2008)

De Souza, Wanderley

Since the discovery of the electron microscope and the development of the initial techniques for the processing of biological samples for electron microscopy, the protozoan Trypanosoma cruzi   has...

Antiproliferative activities of two novel quinuclidine inhibitors against Toxoplasma gondii tachyzoites in vitro (2006)

Martins-Duarte, Érica S., Urbina, Julio A., De Souza, Wanderley, Vommaro, Rossiane C.

Objectives: To study the antiproliferative effects of ER119884 and E5700, two quinuclidine-based inhibitors of squalene synthase (SQS), against Toxoplasma gondii tachyzoites in epithelial cells....

Antiproliferative activities of two novel quinuclidine inhibitors against Toxoplasma gondii tachyzoites in vitro (2006)

Martins-Duarte, Érica S., Urbina, Julio A., De Souza, Wanderley, Vommaro, Rossiane C.

Objectives: To study the antiproliferative effects of ER119884 and E5700, two quinuclidine-based inhibitors of squalene synthase (SQS), against Toxoplasma gondii tachyzoites in epithelial cells....

Programmed cell death in Trypanosoma cruzi induced by Bothrops jararaca venom (2005)

Deolindo, Poliana, Teixeira-Ferreira, André S., Melo, Edésio J.T., Arnholdt, Andrea Cristina Veto, De Souza, Wanderley, Alves, Elias W., ...

Cells die through a programmed process or accidental death, know as apoptosis or necrosis, respectively. Bothrops jararaca is a snake whose venom inhibits the growth of Trypanosoma cruzi epimastigote...

From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality. (2002)

De Souza, Wanderley

Abstract Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These...

Human Antibodies against a Purified Glucosylceramide from Cryptococcus neoformans Inhibit Cell Budding and Fungal Growth

Rodrigues, Marcio L., Travassos, Luiz R., Miranda, Kildare R., Franzen, Anderson J., Rozental, Sonia, De Souza, Wanderley, ...

A major ceramide monohexoside (CMH) was purified from lipidic extracts of Cryptococcus neoformans. This molecule was analyzed by high-performance thin-layer chromatography (HPTLC), gas chromatography...

From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza, Wanderley

Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms...

Ca2+ Content and Expression of an Acidocalcisomal Calcium Pump Are Elevated in Intracellular Forms of Trypanosoma cruzi

Lu, Hong-Gang, Zhong, Li, De Souza, Wanderley, Benchimol, Marlene, Moreno, Silvia, Docampo, Roberto

The survival of a eukaryotic protozoan as an obligate parasite in the interior of a eukaryotic host cell implies its adaptation to an environment with a very different ionic composition from that of...

Chromosome Localization Changes in the Trypanosoma cruzi Nucleus

Faria, Marcella, Mortara, Renato A., Motta, Maria Cristina M., De Souza, Wanderley, Thiry, Marc, ...

Chromosome localization in the interphase nuclei of eukaryotes depends on gene replication and transcription. Little is known about chromosome localization in protozoan parasites such as...

Soluble Factors Released by Toxoplasma gondii-Infected Astrocytes Down-Modulate Nitric Oxide Production by Gamma Interferon-Activated Microglia and Prevent Neuronal Degeneration

Rozenfeld, Claudia, Martinez, Rodrigo, Figueiredo, Rodrigo T., Bozza, Marcelo T., Lima, Flávia R. S., Pires, Ana Lúcia, ...

The maintenance of a benign chronic Toxoplasma gondii infection is mainly dependent on the persistent presence of gamma interferon (IFN-γ) in the central nervous system (CNS). However,...

Mitochondrial Localization of the Mevalonate Pathway Enzyme 3-Hydroxy-3-methyl-glutaryl-CoA Reductase in the Trypanosomatidae

Peña-Diaz, Javier, Montalvetti, Andrea, Flores, Carmen-Lisset, Constán, Aurora, Hurtado-Guerrero, Ramon, De Souza, Wanderley, ...

3-Hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) is a key enzyme in the sterol biosynthesis pathway, but its subcellular distribution in the Trypanosomatidae family is somewhat controversial....

Novel Azasterols as Potential Agents for Treatment of Leishmaniasis and Trypanosomiasis

Lorente, Silvia Orenes, Rodrigues, Juliany C. F., Jiménez, Carmen Jiménez, Joyce-Menekse, Miranda, Rodrigues, Carlos, Croft, Simon L., ...

This paper describes the design and evaluation of novel azasterols as potential compounds for the treatment of leishmaniasis and other diseases caused by trypanosomatid parasites. Azasterols are a...

Antileishmanial Activity of Parthenolide, a Sesquiterpene Lactone Isolated from Tanacetum parthenium

Tiuman, Tatiana Shioji, Ueda-Nakamura, Tânia, Garcia Cortez, Diógenes Aparício, Dias Filho, Benedito Prado, Morgado-Díaz, José Andrés, De Souza, Wanderley, ...

The in vitro activity of parthenolide against Leishmania amazonensis was investigated. Parthenolide is a sesquiterpene lactone purified from the hydroalcoholic extract of aerial parts of Tanacetum...

Cryptic Paraflagellar Rod in Endosymbiont-Containing Kinetoplastid Protozoa

Gadelha, Catarina, Wickstead, Bill, De Souza, Wanderley, Gull, Keith

Cilia and flagella are central to many biological processes in a diverse range of organisms. The kinetoplastid protozoa are very appealing models for the study of flagellar function, particularly in...

Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruzi

Vieira, Mauricio, Rohloff, Peter, Luo, Shuhong, De Souza, Wanderley, Docampo, Roberto

Previous studies in Trypanosoma cruzi, the etiologic agent of Chagas disease, have resulted in the cloning and sequencing of a pair of tandemly linked genes (TcHA1 and TcHA2) that encode P...

Human Antibodies against a Purified Glucosylceramide from Cryptococcus neoformans Inhibit Cell Budding and Fungal Growth

Rodrigues, Marcio L., Travassos, Luiz R., Miranda, Kildare R., Franzen, Anderson J., Rozental, Sonia, De Souza, Wanderley, ...

A major ceramide monohexoside (CMH) was purified from lipidic extracts of Cryptococcus neoformans. This molecule was analyzed by high-performance thin-layer chromatography (HPTLC), gas chromatography...

From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza, Wanderley

Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms...

Ca2+ Content and Expression of an Acidocalcisomal Calcium Pump Are Elevated in Intracellular Forms of Trypanosoma cruzi

Lu, Hong-Gang, Zhong, Li, De Souza, Wanderley, Benchimol, Marlene, Moreno, Silvia, Docampo, Roberto

The survival of a eukaryotic protozoan as an obligate parasite in the interior of a eukaryotic host cell implies its adaptation to an environment with a very different ionic composition from that of...

Chromosome Localization Changes in the Trypanosoma cruzi Nucleus

Faria, Marcella, Mortara, Renato A., Motta, Maria Cristina M., De Souza, Wanderley, Thiry, Marc, ...

Chromosome localization in the interphase nuclei of eukaryotes depends on gene replication and transcription. Little is known about chromosome localization in protozoan parasites such as...

Soluble Factors Released by Toxoplasma gondii-Infected Astrocytes Down-Modulate Nitric Oxide Production by Gamma Interferon-Activated Microglia and Prevent Neuronal Degeneration

Rozenfeld, Claudia, Martinez, Rodrigo, Figueiredo, Rodrigo T., Bozza, Marcelo T., Lima, Flávia R. S., Pires, Ana Lúcia, ...

The maintenance of a benign chronic Toxoplasma gondii infection is mainly dependent on the persistent presence of gamma interferon (IFN-γ) in the central nervous system (CNS). However,...

Mitochondrial Localization of the Mevalonate Pathway Enzyme 3-Hydroxy-3-methyl-glutaryl-CoA Reductase in the Trypanosomatidae

Peña-Diaz, Javier, Montalvetti, Andrea, Flores, Carmen-Lisset, Constán, Aurora, Hurtado-Guerrero, Ramon, De Souza, Wanderley, ...

3-Hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) is a key enzyme in the sterol biosynthesis pathway, but its subcellular distribution in the Trypanosomatidae family is somewhat controversial....

Novel Azasterols as Potential Agents for Treatment of Leishmaniasis and Trypanosomiasis

Lorente, Silvia Orenes, Rodrigues, Juliany C. F., Jiménez, Carmen Jiménez, Joyce-Menekse, Miranda, Rodrigues, Carlos, Croft, Simon L., ...

This paper describes the design and evaluation of novel azasterols as potential compounds for the treatment of leishmaniasis and other diseases caused by trypanosomatid parasites. Azasterols are a...

Antileishmanial Activity of Parthenolide, a Sesquiterpene Lactone Isolated from Tanacetum parthenium

Tiuman, Tatiana Shioji, Ueda-Nakamura, Tânia, Garcia Cortez, Diógenes Aparício, Dias Filho, Benedito Prado, Morgado-Díaz, José Andrés, De Souza, Wanderley, ...

The in vitro activity of parthenolide against Leishmania amazonensis was investigated. Parthenolide is a sesquiterpene lactone purified from the hydroalcoholic extract of aerial parts of Tanacetum...

Cryptic Paraflagellar Rod in Endosymbiont-Containing Kinetoplastid Protozoa

Gadelha, Catarina, Wickstead, Bill, De Souza, Wanderley, Gull, Keith

Cilia and flagella are central to many biological processes in a diverse range of organisms. The kinetoplastid protozoa are very appealing models for the study of flagellar function, particularly in...

Role for a P-type H+-ATPase in the acidification of the endocytic pathway of Trypanosoma cruzi

Vieira, Mauricio, Rohloff, Peter, Luo, Shuhong, De Souza, Wanderley, Docampo, Roberto

Previous studies in Trypanosoma cruzi, the etiologic agent of Chagas disease, have resulted in the cloning and sequencing of a pair of tandemly linked genes (TcHA1 and TcHA2) that encode P...

Early and Late Pathogenic Events of Newborn Mice Encephalitis Experimentally Induced by Itacaiunas and Curionópolis Bracorhabdoviruses Infection

Diniz, José Antonio Picanço, Dos Santos, Zaire Alves, Braga, Marcio Augusto Galvão, Dias, Ádila Liliane Barros, De Almeida Medeiros, Daniele Barbosa, ...

In previous reports we proposed a new genus for Rhabdoviridae and described neurotropic preference and gross neuropathology in newborn albino Swiss mice after Curionopolis and Itacaiunas infections....

In Vitro Activities of ER-119884 and E5700, Two Potent Squalene Synthase Inhibitors, against Leishmania amazonensis: Antiproliferative, Biochemical, and Ultrastructural Effects▿

Fernandes Rodrigues, Juliany Cola, Concepcion, Juan Luis, Rodrigues, Carlos, Caldera, Aura, Urbina, Julio A., De Souza, Wanderley

ER-119884 and E5700, novel arylquinuclidine derivatives developed as cholesterol-lowering agents, were potent in vitro growth inhibitors of both proliferative stages of Leishmania amazonensis, the...

Sterol Biosynthesis Pathway as Target for Anti-trypanosomatid Drugs

De Souza, Wanderley, Rodrigues, Juliany Cola Fernandes

Sterols are constituents of the cellular membranes that are essential for their normal structure and function. In mammalian cells, cholesterol is the main sterol found in the various membranes....

A Trypanosoma cruzi Phosphatidylinositol 3-Kinase (TcVps34) Is Involved in Osmoregulation and Receptor-mediated Endocytosis*S⃞

Schoijet, Alejandra C., Miranda, Kildare, Girard-Dias, Wendell, De Souza, Wanderley, Flawiá, Mirtha M., Torres, Héctor N., ...

Trypanosoma cruzi, the etiological agent of Chagas disease, has the ability to respond to a variety of environmental changes during its life cycle both in the insect vector and in the vertebrate...