Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology (2009)
Jacobs, Pieter P., Geysens, Steven, Vervecken, Wouter, Contreras, Roland, Callewaert, Nico
Here we provide a protocol for engineering the N-glycosylation pathway of the yeast Pichia pastoris. The general strategy consists of the disruption of an endogenous glycosyltransferase gene (OCH1)...
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88 (2007)
Coutinho, Pedro M., Cornell, Michael, Contreras, Roland, Caddick, Mark X., Breestraat, Stefaan, Berg, Marco Van Den, ...
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase...
Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88 (2007)
PEL, HJ, DE WINDE, JH, ARCHER, DB, DYER, PS, HOFMANN, G, SCHAAP, PJ, ...
From laboratory to Phase I/II cancer trials with recombinant biotherapeutics (2007)
TOLNER, B, SMITH, L, HILLYER, T, BHATIA, J, BECKETT, P, ROBSON, L, ...
Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris (2007)
Lannoo, Nausicaä, Vervecken, Wouter, PROOST, P, ROUGE, P, Van Damme, Els
Rating of CCl4-induced rat liver fibrosis by blood serum glycomics (2007)
Desmyter, Liesbeth, Fan, Yedong, Praet, Marleen, JAWORSKI, T, Vervecken, Wouter, De Hemptinne, Bernard, ...
Kogelberg, Heide, Tolner, Berend, Sharma, Surinder K., Lowdell, Mark W, Qureshi, Uzma, Robson, Mathew, ...
MFECP1 is a mannosylated antibody–enzyme fusion protein used in antibody-directed enzyme prodrug therapy (ADEPT). The antibody selectively targets tumor cells and the targeted enzyme converts a...
Kogelberg, Heide, Tolner, Berend, Sharma, Surinder K., Lowdell, Mark W., Qureshi, Uzma, Robson, Mathew, ...
MFECP1 is a mannosylated antibody-enzyme fusion protein used in antibody-directed enzyme prodrug therapy (ADEPT). The antibody selectively targets tumour cells and the targeted enzyme converts a...
Kogelberg, Heide, Tolner, Berend, Sharma, Surinder K., Lowdell, Mark W., Qureshi, Uzma, Robson, Mathew, ...
MFECP1 is a mannosylated antibody-enzyme fusion protein used in antibody-directed enzyme prodrug therapy (ADEPT). The antibody selectively targets tumour cells and the targeted enzyme converts a...
N-glycan engineering in yeasts and fungi: finally human-like glycosylation (2005)
Callewaert, Nico, Vervecken, Wouter, Geysens, Steven, Contreras, Roland
In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris (2004)
Vervecken, Wouter, Kaigorodov, Vladimir, Callewaert, Nico, Geysens, Steven, De Vusser, Kristof, Contreras, Roland
HSAbodies: A new class of engineered antibody-based molecules for targeting cancer (2004)
HUHALOV, A, GRAFF, C, WITTRUP, KD, TOLNER, B, PEDLEY, RB, Vervecken, Wouter, ...
In Vivo Synthesis of Mammalian-Like, Hybrid-Type N-Glycans in Pichia pastoris
Vervecken, Wouter, Kaigorodov, Vladimir, Callewaert, Nico, Geysens, Steven, De Vusser, Kristof, Contreras, Roland
The Pichia pastoris N-glycosylation pathway is only partially homologous to the pathway in human cells. In the Golgi apparatus, human cells synthesize complex oligosaccharides, whereas Pichia cells...
In Vivo Synthesis of Mammalian-Like, Hybrid-Type N-Glycans in Pichia pastoris
Vervecken, Wouter, Kaigorodov, Vladimir, Callewaert, Nico, Geysens, Steven, De Vusser, Kristof, Contreras, Roland
The Pichia pastoris N-glycosylation pathway is only partially homologous to the pathway in human cells. In the Golgi apparatus, human cells synthesize complex oligosaccharides, whereas Pichia cells...
Schmalhorst, Philipp S., Krappmann, Sven, Vervecken, Wouter, Rohde, Manfred, Müller, Meike, Braus, Gerhard H., ...
The filamentous fungus Aspergillus fumigatus is responsible for a lethal disease called invasive aspergillosis that affects immunocompromised patients. This disease, like other human fungal diseases,...